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The map label for this gene is gyrB [H]

Identifier: 154245817

GI number: 154245817

Start: 2110146

End: 2112587

Strand: Reverse

Name: gyrB [H]

Synonym: Xaut_1873

Alternate gene names: 154245817

Gene position: 2112587-2110146 (Counterclockwise)

Preceding gene: 154245818

Following gene: 154245814

Centisome position: 39.79

GC content: 68.02

Gene sequence:

>2442_bases
ATGGCCGAACCCGTGCCTGCCGCGTCCCCCGACGATTACGGCGCCCAGTCCATCCAGGTCCTCAAGGGCCTCGATGCCGT
GCGCAAGCGACCCGGCATGTATATCGGTGATACGGATGACGGCTCGGGCCTCCACCACATGGTCTACGAGGTGGTGGACA
ACGCCATCGACGAGGCGCTGGCCGGCTGGGCCAAGGAAGTCACGGTCACCCTGAACGCCGACGGCTCGGTGACGGTGACC
GACGACGGGCGCGGCATCCCCACCGACATCCACAAGGAAGAGGGCGTCTCGGCGGCCGAGGTCATCATGACCCAGCTGCA
CGCGGGCGGTAAGTTCAACCAGAACTCGTACAAGGTCTCCGGCGGCCTGCACGGGGTTGGCGTCTCGGTGGTGAACGCCC
TGTCCACCACCCTGGACCTGACCATCTGGCGCGATGGCAAGGAGCACTTCATGCGCTTCCGCCACGGCGACGCGGAAGCC
CCGCTGAAGGTGGTGGGCGACGCCCCCGGCGACAAGCGCGGCACCCGGGTGACCTTCACCCCCAGCCCCGAGACCTTCAC
CAAGATCGATTTCGACTACGCCACGCTGGAGCACCGGCTGCGCGAGCTGGCGTTCCTGAATTCCGGCGTGCTCATCATCC
TCACCGACGCGCGCACCGCCGACGTGAAGCGCGAGGAGCTGCATTACGAGGGCGGCGTGGAAGCCTTCGTGAAGTACCTC
GACCGCTCCAAGTCCGCCTTGCTGCAGAAGCCGGTGGTGATCCGCGCCGAGAAGGACGGCATGACCGTGGAAGCCGCCCT
GTCGTGGAACGACGGCTACCACGAAAATATCCTGTGCTTCACCAACAACATTCCCCAGCGGGATCGCGGCACCCACTTCA
CCGGGTTCGCGGCGGCGCTGACCCGGCAGGTGATCGGCTATGCCAACACCTCCGGCATCGCCAAGAAGGAGAAGGTGGAC
CCCACCGGCGAGGATTGCCGCGAAGGCCTGACCGCCGTGCTCTCGGTGAAGGTGCCGGACCCCAAGTTCTCATCCCAGAC
CAAGGACAAGCTGGTGTCCTCCGAGGTCCGCCCGGTGGTGGAATCCCTGGTGAGCGAGGCGCTGTCGGTGTGGTTCGAGG
AGAACCCGGCGGAAGCCAAGAACGTCATCGGCAAGGTGGTGGAAGCCGCCGCCGCACGCGAGGCCGCCCGCAAGGCCCGC
GACCTCACCCGGCGCAAGAGCCCGCTGGACGTTGCCTCCCTGCCCGGCAAGCTAGCCGATTGCCAGGAGCGCGACCCGGC
CAAGTCCGAGATCTTCATCGTCGAGGGTGACTCGGCCGGCGGCTCCGCCAAGCAGGGCCGCGACCGCGCCTTCCAGGCGG
TGCTGCCGTTGCGCGGCAAGATCCTGAACGTGGAGCGCGCGCGCTTCGACAAGATGCTGTCGTCCGAGCAGATCGGCACC
CTGATTACCGCGCTCGGCACCGGCATCGGCCGGGACGACGGCCATTCGGGCGGCTTCGACCTCGCCAAGCTGCGCTACCA
CAAGATCATCATCATGACCGACGCCGACGTGGACGGCGCCCATATCCGCACCCTGCTGCTCACCTTCTTCTTCCGCCAGA
TGCCGGAGCTGGTGGACGCGGGCCACCTCTTCATCGCCCAGCCGCCGCTCTACAAGGTGACGCGCGGCAAGTCCGAGACC
TATCTCAAGGACGAGAAGGCGCTGGAGGACTACCTCATCACCGCGGGGCTCGACGAAGCCTCCCTGCACCTGGCCACGGG
CGAGGTGCGCGCCGGCTCGGACCTCGCCCACGTGGTGGAGGTGTCGCGCCAGATGCGCGCGGCGCTCGGCGCCATGCACC
CGCGCTACAACCGTGCGGTGGCGGAGCAGGCGGCGCTCGCCGGCGCATTCGGCGCCCGGACGCTGCAGGATGAAGCCGCC
GGCATCGCCGCCGCCGCCGAGGTGGCGCTGCGGCTCGACGCCTTGGCGGACGAGACCGAGCGGGGCTGGACCGGCCTGTT
CGACGACGCCGGTTACACCTTCTCCCGCACTGTGCGCGGCGTCGCCGAGGTGGCGCGCATCGACCATGCCTTCGTCGCCT
CCCCTGAGGCGCGGCGGCTCGACAATCTGGCACGGGACCTTGAGGGCGTGTTCACCGCCGCCTGCGTGCTCAAGCGCAAG
AATGACGAGCAGGTGCTGTTCGGTGCCGTGGACCTGTTCGACGCGGTCACCGACACCGGCCGCAAGGGCCTCTCGCTGCA
GCGCTACAAAGGCCTGGGCGAGATGAACCCGGAGCAGCTCTGGGAAACCACGCTGGACGTCAACGCCCGCTCCCTCCTGC
AGGTGCGGGTGAAGGAAGTGGATGCCGCCGACGACCTGTTCAACCGCCTCATGGGCGACACGGTGGAGCCGCGCCGCGAG
TTCATCCAGGAAAACGCCCTGCGCGCCAGCGTGGATGTGTGA

Upstream 100 bases:

>100_bases
ACCGCGCCGGCCAAGGCGTGGAAAGACGCGCGGCCGCCACCAAATAACCGCCACTTTGCGCTAGATTTGCAACAAGTCGC
TCAACGATTCGAGATCAATC

Downstream 100 bases:

>100_bases
TCCGGGTAAGGCGCCCCGCTGGGCGCCTTAATGCCGCGAGAACAGGTCGCGCACCTTGCACAGGGTGGTGGTGACATCGA
AGCGCTGGTCCGGCAGCGCC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 813; Mature: 812

Protein sequence:

>813_residues
MAEPVPAASPDDYGAQSIQVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGWAKEVTVTLNADGSVTVT
DDGRGIPTDIHKEEGVSAAEVIMTQLHAGGKFNQNSYKVSGGLHGVGVSVVNALSTTLDLTIWRDGKEHFMRFRHGDAEA
PLKVVGDAPGDKRGTRVTFTPSPETFTKIDFDYATLEHRLRELAFLNSGVLIILTDARTADVKREELHYEGGVEAFVKYL
DRSKSALLQKPVVIRAEKDGMTVEAALSWNDGYHENILCFTNNIPQRDRGTHFTGFAAALTRQVIGYANTSGIAKKEKVD
PTGEDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVESLVSEALSVWFEENPAEAKNVIGKVVEAAAAREAARKAR
DLTRRKSPLDVASLPGKLADCQERDPAKSEIFIVEGDSAGGSAKQGRDRAFQAVLPLRGKILNVERARFDKMLSSEQIGT
LITALGTGIGRDDGHSGGFDLAKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELVDAGHLFIAQPPLYKVTRGKSET
YLKDEKALEDYLITAGLDEASLHLATGEVRAGSDLAHVVEVSRQMRAALGAMHPRYNRAVAEQAALAGAFGARTLQDEAA
GIAAAAEVALRLDALADETERGWTGLFDDAGYTFSRTVRGVAEVARIDHAFVASPEARRLDNLARDLEGVFTAACVLKRK
NDEQVLFGAVDLFDAVTDTGRKGLSLQRYKGLGEMNPEQLWETTLDVNARSLLQVRVKEVDAADDLFNRLMGDTVEPRRE
FIQENALRASVDV

Sequences:

>Translated_813_residues
MAEPVPAASPDDYGAQSIQVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGWAKEVTVTLNADGSVTVT
DDGRGIPTDIHKEEGVSAAEVIMTQLHAGGKFNQNSYKVSGGLHGVGVSVVNALSTTLDLTIWRDGKEHFMRFRHGDAEA
PLKVVGDAPGDKRGTRVTFTPSPETFTKIDFDYATLEHRLRELAFLNSGVLIILTDARTADVKREELHYEGGVEAFVKYL
DRSKSALLQKPVVIRAEKDGMTVEAALSWNDGYHENILCFTNNIPQRDRGTHFTGFAAALTRQVIGYANTSGIAKKEKVD
PTGEDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVESLVSEALSVWFEENPAEAKNVIGKVVEAAAAREAARKAR
DLTRRKSPLDVASLPGKLADCQERDPAKSEIFIVEGDSAGGSAKQGRDRAFQAVLPLRGKILNVERARFDKMLSSEQIGT
LITALGTGIGRDDGHSGGFDLAKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELVDAGHLFIAQPPLYKVTRGKSET
YLKDEKALEDYLITAGLDEASLHLATGEVRAGSDLAHVVEVSRQMRAALGAMHPRYNRAVAEQAALAGAFGARTLQDEAA
GIAAAAEVALRLDALADETERGWTGLFDDAGYTFSRTVRGVAEVARIDHAFVASPEARRLDNLARDLEGVFTAACVLKRK
NDEQVLFGAVDLFDAVTDTGRKGLSLQRYKGLGEMNPEQLWETTLDVNARSLLQVRVKEVDAADDLFNRLMGDTVEPRRE
FIQENALRASVDV
>Mature_812_residues
AEPVPAASPDDYGAQSIQVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGWAKEVTVTLNADGSVTVTD
DGRGIPTDIHKEEGVSAAEVIMTQLHAGGKFNQNSYKVSGGLHGVGVSVVNALSTTLDLTIWRDGKEHFMRFRHGDAEAP
LKVVGDAPGDKRGTRVTFTPSPETFTKIDFDYATLEHRLRELAFLNSGVLIILTDARTADVKREELHYEGGVEAFVKYLD
RSKSALLQKPVVIRAEKDGMTVEAALSWNDGYHENILCFTNNIPQRDRGTHFTGFAAALTRQVIGYANTSGIAKKEKVDP
TGEDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVESLVSEALSVWFEENPAEAKNVIGKVVEAAAAREAARKARD
LTRRKSPLDVASLPGKLADCQERDPAKSEIFIVEGDSAGGSAKQGRDRAFQAVLPLRGKILNVERARFDKMLSSEQIGTL
ITALGTGIGRDDGHSGGFDLAKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELVDAGHLFIAQPPLYKVTRGKSETY
LKDEKALEDYLITAGLDEASLHLATGEVRAGSDLAHVVEVSRQMRAALGAMHPRYNRAVAEQAALAGAFGARTLQDEAAG
IAAAAEVALRLDALADETERGWTGLFDDAGYTFSRTVRGVAEVARIDHAFVASPEARRLDNLARDLEGVFTAACVLKRKN
DEQVLFGAVDLFDAVTDTGRKGLSLQRYKGLGEMNPEQLWETTLDVNARSLLQVRVKEVDAADDLFNRLMGDTVEPRREF
IQENALRASVDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=563, Percent_Identity=26.4653641207815, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI19913406, Length=566, Percent_Identity=25.0883392226148, Blast_Score=114, Evalue=5e-25,
Organism=Escherichia coli, GI48994957, Length=814, Percent_Identity=57.1253071253071, Blast_Score=869, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=561, Percent_Identity=39.9286987522282, Blast_Score=378, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17535065, Length=538, Percent_Identity=24.5353159851301, Blast_Score=133, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=521, Percent_Identity=23.8003838771593, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324241, Length=609, Percent_Identity=26.2725779967159, Blast_Score=134, Evalue=6e-32,
Organism=Drosophila melanogaster, GI17136538, Length=541, Percent_Identity=27.1719038817006, Blast_Score=138, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 88507; Mature: 88376

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPVPAASPDDYGAQSIQVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEAL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHH
AGWAKEVTVTLNADGSVTVTDDGRGIPTDIHKEEGVSAAEVIMTQLHAGGKFNQNSYKVS
HCCCEEEEEEEECCCCEEECCCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCCCEEEC
GGLHGVGVSVVNALSTTLDLTIWRDGKEHFMRFRHGDAEAPLKVVGDAPGDKRGTRVTFT
CCCCHHHHHHHHHHHHHEEEEEEECCHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEEC
PSPETFTKIDFDYATLEHRLRELAFLNSGVLIILTDARTADVKREELHYEGGVEAFVKYL
CCCCCEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHCCCCCHHHHHHHH
DRSKSALLQKPVVIRAEKDGMTVEAALSWNDGYHENILCFTNNIPQRDRGTHFTGFAAAL
HHHHHHHHCCCEEEEECCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHHHH
TRQVIGYANTSGIAKKEKVDPTGEDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVV
HHHHHHCCCCCCCCCCCCCCCCCHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
ESLVSEALSVWFEENPAEAKNVIGKVVEAAAAREAARKARDLTRRKSPLDVASLPGKLAD
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHC
CQERDPAKSEIFIVEGDSAGGSAKQGRDRAFQAVLPLRGKILNVERARFDKMLSSEQIGT
CCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHH
LITALGTGIGRDDGHSGGFDLAKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELVDA
HHHHHHCCCCCCCCCCCCCCHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
GHLFIAQPPLYKVTRGKSETYLKDEKALEDYLITAGLDEASLHLATGEVRAGSDLAHVVE
CCEEEECCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHH
VSRQMRAALGAMHPRYNRAVAEQAALAGAFGARTLQDEAAGIAAAAEVALRLDALADETE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RGWTGLFDDAGYTFSRTVRGVAEVARIDHAFVASPEARRLDNLARDLEGVFTAACVLKRK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
NDEQVLFGAVDLFDAVTDTGRKGLSLQRYKGLGEMNPEQLWETTLDVNARSLLQVRVKEV
CCCEEEEHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHC
DAADDLFNRLMGDTVEPRREFIQENALRASVDV
CHHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure 
AEPVPAASPDDYGAQSIQVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEAL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHH
AGWAKEVTVTLNADGSVTVTDDGRGIPTDIHKEEGVSAAEVIMTQLHAGGKFNQNSYKVS
HCCCEEEEEEEECCCCEEECCCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCCCEEEC
GGLHGVGVSVVNALSTTLDLTIWRDGKEHFMRFRHGDAEAPLKVVGDAPGDKRGTRVTFT
CCCCHHHHHHHHHHHHHEEEEEEECCHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEEC
PSPETFTKIDFDYATLEHRLRELAFLNSGVLIILTDARTADVKREELHYEGGVEAFVKYL
CCCCCEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHCCCCCHHHHHHHH
DRSKSALLQKPVVIRAEKDGMTVEAALSWNDGYHENILCFTNNIPQRDRGTHFTGFAAAL
HHHHHHHHCCCEEEEECCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHHHH
TRQVIGYANTSGIAKKEKVDPTGEDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVV
HHHHHHCCCCCCCCCCCCCCCCCHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
ESLVSEALSVWFEENPAEAKNVIGKVVEAAAAREAARKARDLTRRKSPLDVASLPGKLAD
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHC
CQERDPAKSEIFIVEGDSAGGSAKQGRDRAFQAVLPLRGKILNVERARFDKMLSSEQIGT
CCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHH
LITALGTGIGRDDGHSGGFDLAKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELVDA
HHHHHHCCCCCCCCCCCCCCHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
GHLFIAQPPLYKVTRGKSETYLKDEKALEDYLITAGLDEASLHLATGEVRAGSDLAHVVE
CCEEEECCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHH
VSRQMRAALGAMHPRYNRAVAEQAALAGAFGARTLQDEAAGIAAAAEVALRLDALADETE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RGWTGLFDDAGYTFSRTVRGVAEVARIDHAFVASPEARRLDNLARDLEGVFTAACVLKRK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
NDEQVLFGAVDLFDAVTDTGRKGLSLQRYKGLGEMNPEQLWETTLDVNARSLLQVRVKEV
CCCEEEEHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHC
DAADDLFNRLMGDTVEPRREFIQENALRASVDV
CHHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226640 [H]