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The map label for this gene is miaB [H]

Identifier: 154245754

GI number: 154245754

Start: 2037848

End: 2039338

Strand: Direct

Name: miaB [H]

Synonym: Xaut_1810

Alternate gene names: 154245754

Gene position: 2037848-2039338 (Clockwise)

Preceding gene: 154245753

Following gene: 154245755

Centisome position: 38.39

GC content: 67.87

Gene sequence:

>1491_bases
ATGAAGGGGGCGACAAGAGCTGGCGTCACGATCGCGTCGCGCCCACGCGCGATGATCGACGGCGACAGAACAGGCAACAT
GCACGAGCCCCGCAAGCTTTATGTGAAGTCATTCGGCTGCCAGATGAACGTCTACGATTCCCATCGTATGGCGGACACGC
TGGCACGCGAGGGCTTCGTGGAGACGCAGGACCCGGCCGAGGCCGATCTTGTCATCCTGAACACCTGCCATATCCGCGAG
AAGGCCGCCGAGAAGGTCTATTCGGAGCTGGGACGCCTGCGCAAACAGAAGCAGGAAGCCGGTTCCGACACCATGATCGC
GGTGGCCGGCTGCGTTGCCCAGGCCGAAGGCGCCGAGATCATGCGCCGCGCGCCGGTGGTGGACCTCGTGGTCGGCCCCC
AGAGCTACCACCGCCTGCCCGAGCTTCTGGCCGAGGCCAAGGCTGGCAAGCGGGTGGTGGACACCGAATTTCCCGCCGAG
GACAAGTTCGACCACCTGCCCGCCCCGACGCGGGCGGCCACACGTTCACGCGGGCCGGCGGCCTTCGTCACCGTGCAGGA
AGGCTGCGACAAGTTCTGCACCTTCTGCGTGGTGCCCTATACCCGCGGCGCCGAAGTCTCCCGCCCGGTCGCGAAGATCA
TGGACGAGGCGGCGCGGCTGGTGGACCAGGGGGTCCGCGAGATCAGCCTCATCGGCCAGAACGTCAACGCCTTCCATGGC
GCGGGACCGGACGGCACGACCTGGAGCCTCGCCCGACTCATGGAGCACATGGCGCGCCTCGATGGCCTTGCCCGGCTGCG
CTACACCACCTCCCATCCCCGCGACATGGACGACGACCTCATCGCCGCTCACCGCGACCTGCCGCAGCTGATGCCCTACC
TGCACCTGCCGGTGCAGTCGGGCTCCGACCGCATCCTCGCCGCCATGAACCGCAAGCACGGCCGGGACGACTTCTTCGCC
ATCATCGAGAAGATGCGGCAGGCGCGGCCGGACATGGCCCTGTCGTCCGACTTCATCGTCGGCTTCCCCGGCGAGAGCGA
GAAGGATTTCGAGGACACCCTCGACCTGGTGCGCCGGGTGGGCTTCGCCAGCGCCTATTCGTTCAAGTACAGCCCCCGGC
CCGGTACGCCTGCGGCCGATCATGACGCGCAGGTGCCGGAGGAGGTGAAGGCCGAGCGCCTCGCCGAGCTTCAGCGGCTG
CTGGAAGCCTCCAAGGCCGCCTTTGACGAGAGCTGCCGCGGCCGCACCTTCGATATCCTGCTGGAGAAGCCCGGCCGCCA
GGCCGGCCAGCTCATCGGCCGCTCGCCCTATCTGCAAAGCGTGGTGGTGAACGATCCGCCCGCCGCCATCGGCGACCTCC
TCACCGTGACCATCACCGATGTGGGGCCCAACAGCCTCGCGGGCGTGCCCGCCGAGGCGAGCGAACCTTCCGTTTCCCAG
TCCCCAGTGTCTTCCGCCCGATCCCGGCCGCTTGCGGCCATGGAGGCTTGA

Upstream 100 bases:

>100_bases
GGCCGCCGCATCGGTGGAACAGCCCTAAGTTCCGCAGCTGGTCGCGCCGGCGCTGTTTCCAACCGGCGCGTTTGCCGGTA
GGATTGCCACCTCACGAACC

Downstream 100 bases:

>100_bases
GCTTGCGCAGATCCGGCAGCGACCGCGACCGTCCCCTCGAACGTTCCTCGGAACGCCCCTCCGAACGCCCGTTGCCGCCC
TCCGGCCCGGCCCGCGCGGC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MKGATRAGVTIASRPRAMIDGDRTGNMHEPRKLYVKSFGCQMNVYDSHRMADTLAREGFVETQDPAEADLVILNTCHIRE
KAAEKVYSELGRLRKQKQEAGSDTMIAVAGCVAQAEGAEIMRRAPVVDLVVGPQSYHRLPELLAEAKAGKRVVDTEFPAE
DKFDHLPAPTRAATRSRGPAAFVTVQEGCDKFCTFCVVPYTRGAEVSRPVAKIMDEAARLVDQGVREISLIGQNVNAFHG
AGPDGTTWSLARLMEHMARLDGLARLRYTTSHPRDMDDDLIAAHRDLPQLMPYLHLPVQSGSDRILAAMNRKHGRDDFFA
IIEKMRQARPDMALSSDFIVGFPGESEKDFEDTLDLVRRVGFASAYSFKYSPRPGTPAADHDAQVPEEVKAERLAELQRL
LEASKAAFDESCRGRTFDILLEKPGRQAGQLIGRSPYLQSVVVNDPPAAIGDLLTVTITDVGPNSLAGVPAEASEPSVSQ
SPVSSARSRPLAAMEA

Sequences:

>Translated_496_residues
MKGATRAGVTIASRPRAMIDGDRTGNMHEPRKLYVKSFGCQMNVYDSHRMADTLAREGFVETQDPAEADLVILNTCHIRE
KAAEKVYSELGRLRKQKQEAGSDTMIAVAGCVAQAEGAEIMRRAPVVDLVVGPQSYHRLPELLAEAKAGKRVVDTEFPAE
DKFDHLPAPTRAATRSRGPAAFVTVQEGCDKFCTFCVVPYTRGAEVSRPVAKIMDEAARLVDQGVREISLIGQNVNAFHG
AGPDGTTWSLARLMEHMARLDGLARLRYTTSHPRDMDDDLIAAHRDLPQLMPYLHLPVQSGSDRILAAMNRKHGRDDFFA
IIEKMRQARPDMALSSDFIVGFPGESEKDFEDTLDLVRRVGFASAYSFKYSPRPGTPAADHDAQVPEEVKAERLAELQRL
LEASKAAFDESCRGRTFDILLEKPGRQAGQLIGRSPYLQSVVVNDPPAAIGDLLTVTITDVGPNSLAGVPAEASEPSVSQ
SPVSSARSRPLAAMEA
>Mature_496_residues
MKGATRAGVTIASRPRAMIDGDRTGNMHEPRKLYVKSFGCQMNVYDSHRMADTLAREGFVETQDPAEADLVILNTCHIRE
KAAEKVYSELGRLRKQKQEAGSDTMIAVAGCVAQAEGAEIMRRAPVVDLVVGPQSYHRLPELLAEAKAGKRVVDTEFPAE
DKFDHLPAPTRAATRSRGPAAFVTVQEGCDKFCTFCVVPYTRGAEVSRPVAKIMDEAARLVDQGVREISLIGQNVNAFHG
AGPDGTTWSLARLMEHMARLDGLARLRYTTSHPRDMDDDLIAAHRDLPQLMPYLHLPVQSGSDRILAAMNRKHGRDDFFA
IIEKMRQARPDMALSSDFIVGFPGESEKDFEDTLDLVRRVGFASAYSFKYSPRPGTPAADHDAQVPEEVKAERLAELQRL
LEASKAAFDESCRGRTFDILLEKPGRQAGQLIGRSPYLQSVVVNDPPAAIGDLLTVTITDVGPNSLAGVPAEASEPSVSQ
SPVSSARSRPLAAMEA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=477, Percent_Identity=33.9622641509434, Blast_Score=285, Evalue=6e-77,
Organism=Homo sapiens, GI28872782, Length=477, Percent_Identity=33.9622641509434, Blast_Score=285, Evalue=8e-77,
Organism=Homo sapiens, GI93277076, Length=465, Percent_Identity=27.3118279569892, Blast_Score=192, Evalue=8e-49,
Organism=Escherichia coli, GI1786882, Length=441, Percent_Identity=51.0204081632653, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1787057, Length=454, Percent_Identity=31.2775330396476, Blast_Score=200, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17553146, Length=465, Percent_Identity=32.258064516129, Blast_Score=252, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI71996771, Length=392, Percent_Identity=28.0612244897959, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI21356207, Length=483, Percent_Identity=32.0910973084886, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI19922432, Length=454, Percent_Identity=27.5330396475771, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 54177; Mature: 54177

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGATRAGVTIASRPRAMIDGDRTGNMHEPRKLYVKSFGCQMNVYDSHRMADTLAREGFV
CCCCCCCCEEEECCCCCEECCCCCCCCCCCHHHHHHHCCCEEECCCHHHHHHHHHHCCCC
ETQDPAEADLVILNTCHIREKAAEKVYSELGRLRKQKQEAGSDTMIAVAGCVAQAEGAEI
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCHHHH
MRRAPVVDLVVGPQSYHRLPELLAEAKAGKRVVDTEFPAEDKFDHLPAPTRAATRSRGPA
HHHCCEEEEEECCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHCCCCCE
AFVTVQEGCDKFCTFCVVPYTRGAEVSRPVAKIMDEAARLVDQGVREISLIGQNVNAFHG
EEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AGPDGTTWSLARLMEHMARLDGLARLRYTTSHPRDMDDDLIAAHRDLPQLMPYLHLPVQS
CCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHCHHHHHHHHCCCCCC
GSDRILAAMNRKHGRDDFFAIIEKMRQARPDMALSSDFIVGFPGESEKDFEDTLDLVRRV
CCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEECCCEEEECCCCCCCCHHHHHHHHHHH
GFASAYSFKYSPRPGTPAADHDAQVPEEVKAERLAELQRLLEASKAAFDESCRGRTFDIL
CCHHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
LEKPGRQAGQLIGRSPYLQSVVVNDPPAAIGDLLTVTITDVGPNSLAGVPAEASEPSVSQ
ECCCCHHHHHHHCCCCCCCEEEECCCCHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCC
SPVSSARSRPLAAMEA
CHHHHHHCCCCHHCCC
>Mature Secondary Structure
MKGATRAGVTIASRPRAMIDGDRTGNMHEPRKLYVKSFGCQMNVYDSHRMADTLAREGFV
CCCCCCCCEEEECCCCCEECCCCCCCCCCCHHHHHHHCCCEEECCCHHHHHHHHHHCCCC
ETQDPAEADLVILNTCHIREKAAEKVYSELGRLRKQKQEAGSDTMIAVAGCVAQAEGAEI
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCHHHH
MRRAPVVDLVVGPQSYHRLPELLAEAKAGKRVVDTEFPAEDKFDHLPAPTRAATRSRGPA
HHHCCEEEEEECCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHCCCCCE
AFVTVQEGCDKFCTFCVVPYTRGAEVSRPVAKIMDEAARLVDQGVREISLIGQNVNAFHG
EEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AGPDGTTWSLARLMEHMARLDGLARLRYTTSHPRDMDDDLIAAHRDLPQLMPYLHLPVQS
CCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHCHHHHHHHHCCCCCC
GSDRILAAMNRKHGRDDFFAIIEKMRQARPDMALSSDFIVGFPGESEKDFEDTLDLVRRV
CCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEECCCEEEECCCCCCCCHHHHHHHHHHH
GFASAYSFKYSPRPGTPAADHDAQVPEEVKAERLAELQRLLEASKAAFDESCRGRTFDIL
CCHHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
LEKPGRQAGQLIGRSPYLQSVVVNDPPAAIGDLLTVTITDVGPNSLAGVPAEASEPSVSQ
ECCCCHHHHHHHCCCCCCCEEEECCCCHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCC
SPVSSARSRPLAAMEA
CHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA