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The map label for this gene is fixK [H]

Identifier: 154245690

GI number: 154245690

Start: 1965990

End: 1966700

Strand: Direct

Name: fixK [H]

Synonym: Xaut_1746

Alternate gene names: 154245690

Gene position: 1965990-1966700 (Clockwise)

Preceding gene: 154245684

Following gene: 154245703

Centisome position: 37.03

GC content: 68.78

Gene sequence:

>711_bases
ATGCCGAGCCCCGCTGCAGCGATCGCCCAGAAGGCTTACGCTCACCCCATGACCGCTCCGCGCTGGGGCGACGAGGCCGA
GAGCCGCTCCCCCATCGATGTGGTGGCCCGCCTCGGTATCGTGGCTTCATATGCCCGCAACGCTGAGATCTTCGGCGAAG
ATCAGCCCGCCGACCATCTCTACATCGTTCTGTCGGGCGCCGTGCGCATCTGCAAGCTGCTCGGCGACGGACGCCGCCAG
ATCGAGACCTTCTGCCTCGCCGGCGACGTGTTCGGCTGGGAGATGGAGCCCCGCCACCGCTTCTCCGCCGAGGCCGTGAG
CGAATGCAAGGTGGTGCGCGTCAAGCGGAGCATCCTGTTCTCCCGCGCCGCCGATGACGCCGAGCTGGCCCATGCCCTGT
GGGCGCTGACCGCCTCCGAGCTTGGCCGGGCGCAGTCCCATCTTCTGGTGCTCGGCCGCAAGACCGCGCAGGAGCGGGTC
GCCACCTTCCTGCTCGACATGGCGGCGCGCGCCGGCTCCGGCCAGGGCACGAGCGGAATCGAGGTGACGCTGCCCATGTC
GCGTCAGGACATCGCCGACTTCCTCGGCCTCACCATCGAGACGGTGTCGCGGACCCTGACCCATCTCGAGGAGATTTCGG
CCATCGCCCTTCCCTCTTCCCGCCGCGTCGTGTTGCGCGACGGAGCAGCGCTGCGGCGCCTGAATTCCTGA

Upstream 100 bases:

>100_bases
GTAAAACTACTTAAGGGCACCGACTTAAGTGTTCGATCCGAATTTTGCGATGCGTCAATGCCGGTTATCCCTTTCTGCAA
CGTGATGGAAGGGTAAACCG

Downstream 100 bases:

>100_bases
CCGGTTTCCCCCGGCGTGCCGCATCATCTGCGGCATACCTCCTTCGGCACGCGCTTCCGGGCGCGTGCCTTTTCTTTTTT
GGGGTGAAGTAAGGCGAGCC

Product: CRP/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MPSPAAAIAQKAYAHPMTAPRWGDEAESRSPIDVVARLGIVASYARNAEIFGEDQPADHLYIVLSGAVRICKLLGDGRRQ
IETFCLAGDVFGWEMEPRHRFSAEAVSECKVVRVKRSILFSRAADDAELAHALWALTASELGRAQSHLLVLGRKTAQERV
ATFLLDMAARAGSGQGTSGIEVTLPMSRQDIADFLGLTIETVSRTLTHLEEISAIALPSSRRVVLRDGAALRRLNS

Sequences:

>Translated_236_residues
MPSPAAAIAQKAYAHPMTAPRWGDEAESRSPIDVVARLGIVASYARNAEIFGEDQPADHLYIVLSGAVRICKLLGDGRRQ
IETFCLAGDVFGWEMEPRHRFSAEAVSECKVVRVKRSILFSRAADDAELAHALWALTASELGRAQSHLLVLGRKTAQERV
ATFLLDMAARAGSGQGTSGIEVTLPMSRQDIADFLGLTIETVSRTLTHLEEISAIALPSSRRVVLRDGAALRRLNS
>Mature_235_residues
PSPAAAIAQKAYAHPMTAPRWGDEAESRSPIDVVARLGIVASYARNAEIFGEDQPADHLYIVLSGAVRICKLLGDGRRQI
ETFCLAGDVFGWEMEPRHRFSAEAVSECKVVRVKRSILFSRAADDAELAHALWALTASELGRAQSHLLVLGRKTAQERVA
TFLLDMAARAGSGQGTSGIEVTLPMSRQDIADFLGLTIETVSRTLTHLEEISAIALPSSRRVVLRDGAALRRLNS

Specific function: FixK is a protein that regulates nitrogen fixation genes both positively and negatively. FixK is a positive regulator of nifA expression. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=177, Percent_Identity=29.3785310734463, Blast_Score=78, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25618; Mature: 25486

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPAAAIAQKAYAHPMTAPRWGDEAESRSPIDVVARLGIVASYARNAEIFGEDQPADHL
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
YIVLSGAVRICKLLGDGRRQIETFCLAGDVFGWEMEPRHRFSAEAVSECKVVRVKRSILF
EEEHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHH
SRAADDAELAHALWALTASELGRAQSHLLVLGRKTAQERVATFLLDMAARAGSGQGTSGI
HHCCCHHHHHHHHHHHHHHHHCCHHHHEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCE
EVTLPMSRQDIADFLGLTIETVSRTLTHLEEISAIALPSSRRVVLRDGAALRRLNS
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHCCC
>Mature Secondary Structure 
PSPAAAIAQKAYAHPMTAPRWGDEAESRSPIDVVARLGIVASYARNAEIFGEDQPADHL
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
YIVLSGAVRICKLLGDGRRQIETFCLAGDVFGWEMEPRHRFSAEAVSECKVVRVKRSILF
EEEHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHH
SRAADDAELAHALWALTASELGRAQSHLLVLGRKTAQERVATFLLDMAARAGSGQGTSGI
HHCCCHHHHHHHHHHHHHHHHCCHHHHEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCE
EVTLPMSRQDIADFLGLTIETVSRTLTHLEEISAIALPSSRRVVLRDGAALRRLNS
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1766374 [H]