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The map label for this gene is otsB [H]

Identifier: 154245666

GI number: 154245666

Start: 1937701

End: 1938528

Strand: Reverse

Name: otsB [H]

Synonym: Xaut_1722

Alternate gene names: 154245666

Gene position: 1938528-1937701 (Counterclockwise)

Preceding gene: 154245669

Following gene: 154245665

Centisome position: 36.51

GC content: 71.98

Gene sequence:

>828_bases
GTGCTGACCCAACTGCACCCGTCCGCGACCCGTGAAGCTTCGGCCGGACAGGCGTTCGAGGGGCACCGGCCCGACGCGCC
GTTCCCGGCGGCCTGCGCGGGCGTACGCGCCGACACCCATGCTTTCTTCCTCGACGTGGACGGCAGCCTGATCGACATCG
CGGACCACCCCGACGCCGTGGAGGTTCCCGAGGATCTCCCGCAGCTTCTGGCCGCGCTGGCGGAGCAGGCCGACGGCGCG
CTGGCCCTCGTCAGCGGGCGGACCATCCAGGCCCTCGACATTCTGCTGCACCCGTTCGGCTTCGCGGCGGCGGGTAGCCA
TGGCGCCCAGATGCGGCTTGAGCCCGACGGCAGGATCCTCTCCGCGCCGGCGCTGGACACCGCGCTTGCCGACGCGGTGA
AAGAGGTGGCGGACGGGTTCGACGGCGTGTTCGCCGAGGACAAGGGCACCGCCATCGCGGTTCATTACCGTGCCGTGCCC
GATGCTGCGCCCTCCCTCAGGCGCGCGCTGCTGGAGCTCCTCCGCGCGCGGCAGGATGTGCGCCTGATGCCTGGCCATTG
CGTGTTCGAGGTGAAGCCCGCCAGCCTCGACAAGGGCACGGCGGTGGCGGATTTCATGGCCCGCGCCCCGTTTGCCGGGC
GTGTTCCCGTCTTCATCGGCGACGATGTCACCGACGAGGCCGGTTTTGGCGCCGTCGCCGCGCGCGGGGGCCTTGCCCTT
GCGGTCGGTTCGCCGCGACCGGGCACCCAGGGGGTGCTTGCCGATCCCCGGTCGGTCCGGGCTTTCCTCACCCACCTCGC
GGCGGTGCCGCAGCAACGACGGAGGTGA

Upstream 100 bases:

>100_bases
GCAGCTTGCGCAGGCATGGCGGCATTGCGTCAAACATGCGGGGGAACCATGGCGAGGGCTTGAGCGTTGAGATGACTGTA
ATGGGATAAGGAGATGGTTT

Downstream 100 bases:

>100_bases
CCCTCGTGTCTCGCATCGTGGTCGTATCCAATCGTGTGGCGGTCCCCCAGCGCTCGGGGGTCACGGCCGGCGGACTGGCG
GTGGCGGTCAACGCCGCGCT

Product: HAD family hydrolase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MLTQLHPSATREASAGQAFEGHRPDAPFPAACAGVRADTHAFFLDVDGSLIDIADHPDAVEVPEDLPQLLAALAEQADGA
LALVSGRTIQALDILLHPFGFAAAGSHGAQMRLEPDGRILSAPALDTALADAVKEVADGFDGVFAEDKGTAIAVHYRAVP
DAAPSLRRALLELLRARQDVRLMPGHCVFEVKPASLDKGTAVADFMARAPFAGRVPVFIGDDVTDEAGFGAVAARGGLAL
AVGSPRPGTQGVLADPRSVRAFLTHLAAVPQQRRR

Sequences:

>Translated_275_residues
MLTQLHPSATREASAGQAFEGHRPDAPFPAACAGVRADTHAFFLDVDGSLIDIADHPDAVEVPEDLPQLLAALAEQADGA
LALVSGRTIQALDILLHPFGFAAAGSHGAQMRLEPDGRILSAPALDTALADAVKEVADGFDGVFAEDKGTAIAVHYRAVP
DAAPSLRRALLELLRARQDVRLMPGHCVFEVKPASLDKGTAVADFMARAPFAGRVPVFIGDDVTDEAGFGAVAARGGLAL
AVGSPRPGTQGVLADPRSVRAFLTHLAAVPQQRRR
>Mature_275_residues
MLTQLHPSATREASAGQAFEGHRPDAPFPAACAGVRADTHAFFLDVDGSLIDIADHPDAVEVPEDLPQLLAALAEQADGA
LALVSGRTIQALDILLHPFGFAAAGSHGAQMRLEPDGRILSAPALDTALADAVKEVADGFDGVFAEDKGTAIAVHYRAVP
DAAPSLRRALLELLRARQDVRLMPGHCVFEVKPASLDKGTAVADFMARAPFAGRVPVFIGDDVTDEAGFGAVAARGGLAL
AVGSPRPGTQGVLADPRSVRAFLTHLAAVPQQRRR

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=238, Percent_Identity=38.655462184874, Blast_Score=160, Evalue=1e-40,
Organism=Drosophila melanogaster, GI19920676, Length=213, Percent_Identity=30.0469483568075, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI221473368, Length=197, Percent_Identity=29.4416243654822, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20129307, Length=197, Percent_Identity=29.4416243654822, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582490, Length=197, Percent_Identity=29.4416243654822, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 28679; Mature: 28679

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTQLHPSATREASAGQAFEGHRPDAPFPAACAGVRADTHAFFLDVDGSLIDIADHPDAV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEEECCCCEEEECCCCCCC
EVPEDLPQLLAALAEQADGALALVSGRTIQALDILLHPFGFAAAGSHGAQMRLEPDGRIL
CCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCEEEECCCCCEE
SAPALDTALADAVKEVADGFDGVFAEDKGTAIAVHYRAVPDAAPSLRRALLELLRARQDV
ECCHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCC
RLMPGHCVFEVKPASLDKGTAVADFMARAPFAGRVPVFIGDDVTDEAGFGAVAARGGLAL
EECCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCEEEECCCEEE
AVGSPRPGTQGVLADPRSVRAFLTHLAAVPQQRRR
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTQLHPSATREASAGQAFEGHRPDAPFPAACAGVRADTHAFFLDVDGSLIDIADHPDAV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEEECCCCEEEECCCCCCC
EVPEDLPQLLAALAEQADGALALVSGRTIQALDILLHPFGFAAAGSHGAQMRLEPDGRIL
CCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCEEEECCCCCEE
SAPALDTALADAVKEVADGFDGVFAEDKGTAIAVHYRAVPDAAPSLRRALLELLRARQDV
ECCHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCC
RLMPGHCVFEVKPASLDKGTAVADFMARAPFAGRVPVFIGDDVTDEAGFGAVAARGGLAL
EECCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCEEEECCCEEE
AVGSPRPGTQGVLADPRSVRAFLTHLAAVPQQRRR
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]