Click here to switch to the map view.

The map label for this gene is otsA [H]

Identifier: 154245665

GI number: 154245665

Start: 1936217

End: 1937695

Strand: Reverse

Name: otsA [H]

Synonym: Xaut_1721

Alternate gene names: 154245665

Gene position: 1937695-1936217 (Counterclockwise)

Preceding gene: 154245666

Following gene: 154245664

Centisome position: 36.5

GC content: 67.68

Gene sequence:

>1479_bases
GTGTCTCGCATCGTGGTCGTATCCAATCGTGTGGCGGTCCCCCAGCGCTCGGGGGTCACGGCCGGCGGACTGGCGGTGGC
GGTCAACGCCGCGCTCAAGGACAAGGGCGGGATCTGGTTCGGCTGGAGCGGGCGCATCTGCCCGGAACCGTCGGAAATCC
CGGAAGTGACGCGCAAGGCCGGCATCACCTATGCGGTGGTGGACCTGAAGGCCGAGGACCATCAGGAATATTACAACGGT
TTCGCCAACCGGGTGCTGTGGCCGGTGCTGCACTACCGGGTGGATCTCTCCGAATTCTTCCGCTCGGATCTGTCGGGCTA
CATCCGGGTGAACCGCATGTTCGCGGATCACCTGGAGAAGATCATCCGCCCCGACGACATCCTGTGGATCCACGACTACC
ACATGATGCCGCTCGCCAAATATCTGCGCGAGCGCGGCCACAAGAACCGCATCGGCTTCTTCCTGCACATTCCCATGCCG
CCGTCGGATATCGTCCAGGCCCTGCCGCAGCACGGCGAGACCGTGGGCGCGCTGGCCTATTACGATCTCGTCGGCTTCCA
GACCGAGGGCGACCGGGACAATTTCGCCCATTACCTGGAGATCCGCGGCGGCCAGCTGACGCCCGACGGCACCGGCGTCG
ATATCGGCGGGCGGCGGGTCAGGCTCGGCACCTTCCCGGTGGGCATCGAGACCGCGGCCTTTGCCCGGCGGGCGCGGCAT
GCCAGCCGCGCCGCCTTCGTGAAATCGTTCCGCGACAGTCTCGTGGGCAAGAAGCTCATCATCGGCGTGGACCGGCTGGA
TTATTCCAAGGGCATCCCCAACCGGCTCGAGGCCTACGAGCAATTCCTCAAGACCCAGCCCGACTGGCACGAGCGCGTCA
CCTTCGTGCAGATCACCCCCAAGAGCCGCACCGACGTGCCGGAATATCAGGAGATGGACCGCTACGTCTCCCACAAGGCC
GGGCAGATCAACGGCGCCTATTCCGACGTGTCGTGGACCCCGCTGCGCTACGTGAACCGCACCTATTCGCGCTCGGCGCT
CGCGGGCTTCTACCGGGCCGCCCACGTGGCCATGGTGACGCCCCTGCGCGACGGCATGAACCTTGTCGCCAAGGAGTTTG
TGGCGGCGCAGGACCCCGCCGATCCCGGCGTGCTCATCCTGTCCCAGTTCGCCGGGGCGGCGGCCGAGATGGGCACGGGC
GCGCTCATCGTGAACCCGCACGATACGGAAGGCATGGCCAATGCCCTGCGCCGGGCGCTGGAGATGCCGCTGGCGGAGCG
GCGCGAGCGCTATGCCCGGATGATGCAGGTGCTGGAGGCTCGCGACATCGACCGCTGGGCGGCGGACTATCTCGCCGCGC
TGGCGCCGGAAGCCGCGACGGTGGAGCGGCGCGGATTGAGCCTGCCGCGGGCGGCCATCCGCAGCGTGGTGCGCCTGCCC
GGCGCCTGGCGCTCCGATTTCGTGCCACGGGGGGCGTGA

Upstream 100 bases:

>100_bases
CGCGACCGGGCACCCAGGGGGTGCTTGCCGATCCCCGGTCGGTCCGGGCTTTCCTCACCCACCTCGCGGCGGTGCCGCAG
CAACGACGGAGGTGACCCTC

Downstream 100 bases:

>100_bases
GCCGGCGGTAGATGGCCCCCGGCGCCCGGTTGCCGGCCGAGCGCCTTGCCGCTATAGCAACGCAAGCGGCGAAGCCTGCC
GCGAAGGCCCCTGAGCCTTC

Product: alpha,alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSRIVVVSNRVAVPQRSGVTAGGLAVAVNAALKDKGGIWFGWSGRICPEPSEIPEVTRKAGITYAVVDLKAEDHQEYYNG
FANRVLWPVLHYRVDLSEFFRSDLSGYIRVNRMFADHLEKIIRPDDILWIHDYHMMPLAKYLRERGHKNRIGFFLHIPMP
PSDIVQALPQHGETVGALAYYDLVGFQTEGDRDNFAHYLEIRGGQLTPDGTGVDIGGRRVRLGTFPVGIETAAFARRARH
ASRAAFVKSFRDSLVGKKLIIGVDRLDYSKGIPNRLEAYEQFLKTQPDWHERVTFVQITPKSRTDVPEYQEMDRYVSHKA
GQINGAYSDVSWTPLRYVNRTYSRSALAGFYRAAHVAMVTPLRDGMNLVAKEFVAAQDPADPGVLILSQFAGAAAEMGTG
ALIVNPHDTEGMANALRRALEMPLAERRERYARMMQVLEARDIDRWAADYLAALAPEAATVERRGLSLPRAAIRSVVRLP
GAWRSDFVPRGA

Sequences:

>Translated_492_residues
MSRIVVVSNRVAVPQRSGVTAGGLAVAVNAALKDKGGIWFGWSGRICPEPSEIPEVTRKAGITYAVVDLKAEDHQEYYNG
FANRVLWPVLHYRVDLSEFFRSDLSGYIRVNRMFADHLEKIIRPDDILWIHDYHMMPLAKYLRERGHKNRIGFFLHIPMP
PSDIVQALPQHGETVGALAYYDLVGFQTEGDRDNFAHYLEIRGGQLTPDGTGVDIGGRRVRLGTFPVGIETAAFARRARH
ASRAAFVKSFRDSLVGKKLIIGVDRLDYSKGIPNRLEAYEQFLKTQPDWHERVTFVQITPKSRTDVPEYQEMDRYVSHKA
GQINGAYSDVSWTPLRYVNRTYSRSALAGFYRAAHVAMVTPLRDGMNLVAKEFVAAQDPADPGVLILSQFAGAAAEMGTG
ALIVNPHDTEGMANALRRALEMPLAERRERYARMMQVLEARDIDRWAADYLAALAPEAATVERRGLSLPRAAIRSVVRLP
GAWRSDFVPRGA
>Mature_491_residues
SRIVVVSNRVAVPQRSGVTAGGLAVAVNAALKDKGGIWFGWSGRICPEPSEIPEVTRKAGITYAVVDLKAEDHQEYYNGF
ANRVLWPVLHYRVDLSEFFRSDLSGYIRVNRMFADHLEKIIRPDDILWIHDYHMMPLAKYLRERGHKNRIGFFLHIPMPP
SDIVQALPQHGETVGALAYYDLVGFQTEGDRDNFAHYLEIRGGQLTPDGTGVDIGGRRVRLGTFPVGIETAAFARRARHA
SRAAFVKSFRDSLVGKKLIIGVDRLDYSKGIPNRLEAYEQFLKTQPDWHERVTFVQITPKSRTDVPEYQEMDRYVSHKAG
QINGAYSDVSWTPLRYVNRTYSRSALAGFYRAAHVAMVTPLRDGMNLVAKEFVAAQDPADPGVLILSQFAGAAAEMGTGA
LIVNPHDTEGMANALRRALEMPLAERRERYARMMQVLEARDIDRWAADYLAALAPEAATVERRGLSLPRAAIRSVVRLPG
AWRSDFVPRGA

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=467, Percent_Identity=47.1092077087794, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71987012, Length=419, Percent_Identity=30.0715990453461, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI115533560, Length=419, Percent_Identity=28.6396181384248, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI115533558, Length=419, Percent_Identity=28.6396181384248, Blast_Score=171, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6319602, Length=470, Percent_Identity=32.7659574468085, Blast_Score=275, Evalue=8e-75,
Organism=Saccharomyces cerevisiae, GI6323537, Length=422, Percent_Identity=31.042654028436, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6323917, Length=439, Percent_Identity=27.3348519362187, Blast_Score=189, Evalue=8e-49,
Organism=Saccharomyces cerevisiae, GI6320279, Length=406, Percent_Identity=25.615763546798, Blast_Score=172, Evalue=7e-44,
Organism=Drosophila melanogaster, GI19920676, Length=487, Percent_Identity=37.1663244353183, Blast_Score=292, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 55089; Mature: 54957

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIVVVSNRVAVPQRSGVTAGGLAVAVNAALKDKGGIWFGWSGRICPEPSEIPEVTRKA
CCEEEEEECCCCCCCCCCCCCCCHHEEEEHHHCCCCCEEECCCCCCCCCCHHHHHHHHHC
GITYAVVDLKAEDHQEYYNGFANRVLWPVLHYRVDLSEFFRSDLSGYIRVNRMFADHLEK
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHEEHHHHHHHHHHH
IIRPDDILWIHDYHMMPLAKYLRERGHKNRIGFFLHIPMPPSDIVQALPQHGETVGALAY
HCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHH
YDLVGFQTEGDRDNFAHYLEIRGGQLTPDGTGVDIGGRRVRLGTFPVGIETAAFARRARH
HHHHCCCCCCCCCCCEEEEEEECCEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHH
ASRAAFVKSFRDSLVGKKLIIGVDRLDYSKGIPNRLEAYEQFLKTQPDWHERVTFVQITP
HHHHHHHHHHHHHHCCCEEEEEECHHCCCCCCCHHHHHHHHHHCCCCCHHHCEEEEEECC
KSRTDVPEYQEMDRYVSHKAGQINGAYSDVSWTPLRYVNRTYSRSALAGFYRAAHVAMVT
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PLRDGMNLVAKEFVAAQDPADPGVLILSQFAGAAAEMGTGALIVNPHDTEGMANALRRAL
CHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCCHHHHCCCCEEEECCCCCHHHHHHHHHHH
EMPLAERRERYARMMQVLEARDIDRWAADYLAALAPEAATVERRGLSLPRAAIRSVVRLP
HCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHCC
GAWRSDFVPRGA
CCCHHCCCCCCC
>Mature Secondary Structure 
SRIVVVSNRVAVPQRSGVTAGGLAVAVNAALKDKGGIWFGWSGRICPEPSEIPEVTRKA
CEEEEEECCCCCCCCCCCCCCCHHEEEEHHHCCCCCEEECCCCCCCCCCHHHHHHHHHC
GITYAVVDLKAEDHQEYYNGFANRVLWPVLHYRVDLSEFFRSDLSGYIRVNRMFADHLEK
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHEEHHHHHHHHHHH
IIRPDDILWIHDYHMMPLAKYLRERGHKNRIGFFLHIPMPPSDIVQALPQHGETVGALAY
HCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHH
YDLVGFQTEGDRDNFAHYLEIRGGQLTPDGTGVDIGGRRVRLGTFPVGIETAAFARRARH
HHHHCCCCCCCCCCCEEEEEEECCEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHH
ASRAAFVKSFRDSLVGKKLIIGVDRLDYSKGIPNRLEAYEQFLKTQPDWHERVTFVQITP
HHHHHHHHHHHHHHCCCEEEEEECHHCCCCCCCHHHHHHHHHHCCCCCHHHCEEEEEECC
KSRTDVPEYQEMDRYVSHKAGQINGAYSDVSWTPLRYVNRTYSRSALAGFYRAAHVAMVT
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PLRDGMNLVAKEFVAAQDPADPGVLILSQFAGAAAEMGTGALIVNPHDTEGMANALRRAL
CHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCCHHHHCCCCEEEECCCCCHHHHHHHHHHH
EMPLAERRERYARMMQVLEARDIDRWAADYLAALAPEAATVERRGLSLPRAAIRSVVRLP
HCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHCC
GAWRSDFVPRGA
CCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]