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The map label for this gene is tktB [C]

Identifier: 154245656

GI number: 154245656

Start: 1927552

End: 1928388

Strand: Direct

Name: tktB [C]

Synonym: Xaut_1712

Alternate gene names: 154245656

Gene position: 1927552-1928388 (Clockwise)

Preceding gene: 154245655

Following gene: 154245657

Centisome position: 36.31

GC content: 66.31

Gene sequence:

>837_bases
ATGCCATCGCCCAATGAAACGCCGTCCTTCTCCCATCTGCGGGACAAAGCCCTGCAGCTGCGCCGCAACATGCTGATCCA
AGCCGCCGGCAAGGGGCAGGGTTATCTCGGACAGGGGTTCGGCCTCGCCGAATTCTTTGCCGCCCTGTATTTCCACGAAC
TGAACTTCGATCCCAAGTACCCGCAGGCGCCGGACCGCGACCGTTTCTACATGTCCACCGGCCATTACTCGATCGCCCTG
TGGGCGGTGTTCGCCGAGATCGGCCTCATCCCGAAGGACACGCTCACCACCTACGGCGCCGACGAGAGCCCGCTGGAAAT
GAGCACCATCGAAGGCCACGTGCCGGGGGTGGAGATGACCGGCGGGTCCCTCGGTCACGGCCTCGGCATCGCGGCGGGGG
CGGCGCTAGGCCTGCGGATCCAGAACAATCCGGCGCGGGTGTTCGTGGAGATTTCCGACGGCGAACTGCAGGAAGGCTCC
ACCTGGGAAGCCGCCACGGCGGCCAGCGCCTTCCGCCTCGATAACCTCGTCTGCTTCAACGACTGCAACGGCATCCAGGC
CGACGGCGCGCTGGTGGTGCCCATGGAGCCGGTGGCCGACAAATGGCGCGCCTTCGGCTGGGACGTGCAGGAGGTGGACG
GCAACGCGCTGGAGGAACTCATCGGAGCCATGCACTGGGCCCGCGCCCGCCGCGGCAAGCCGAAAGTCATCGTCATGCGC
ACCCGGCCGGGCCGGGGCATCGCCCGCCTCGAAGCCCGCGAGCAGGCCCATTTCATCCGGGTCGAAGGCAACGAATGGGA
CACGCTCGGGCGTGAACTGGAGCTGGAAAATGCTTAA

Upstream 100 bases:

>100_bases
CAGCTTCCTCGTCTCCCCCGAGGCCCGCTACGTCACCGGCGCCATGCTTCCGGTGGACGGCGGCTATTCCATCTCCTGAA
AAATCCGCGAGAAGCCCGCC

Downstream 100 bases:

>100_bases
GGGCCGCACTCTGGGCATGGGTGAGCTGGAAGCGGTGGAGAACCGCGCCTCAAAGCTCGCCCCCTTCGCCACCGGCGCCA
TCGCCGCACAGGAGGCGCAC

Product: transketolase domain-containing protein

Products: D-Fructose 6-phosphate; Glyceraldehyde 3-phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MPSPNETPSFSHLRDKALQLRRNMLIQAAGKGQGYLGQGFGLAEFFAALYFHELNFDPKYPQAPDRDRFYMSTGHYSIAL
WAVFAEIGLIPKDTLTTYGADESPLEMSTIEGHVPGVEMTGGSLGHGLGIAAGAALGLRIQNNPARVFVEISDGELQEGS
TWEAATAASAFRLDNLVCFNDCNGIQADGALVVPMEPVADKWRAFGWDVQEVDGNALEELIGAMHWARARRGKPKVIVMR
TRPGRGIARLEAREQAHFIRVEGNEWDTLGRELELENA

Sequences:

>Translated_278_residues
MPSPNETPSFSHLRDKALQLRRNMLIQAAGKGQGYLGQGFGLAEFFAALYFHELNFDPKYPQAPDRDRFYMSTGHYSIAL
WAVFAEIGLIPKDTLTTYGADESPLEMSTIEGHVPGVEMTGGSLGHGLGIAAGAALGLRIQNNPARVFVEISDGELQEGS
TWEAATAASAFRLDNLVCFNDCNGIQADGALVVPMEPVADKWRAFGWDVQEVDGNALEELIGAMHWARARRGKPKVIVMR
TRPGRGIARLEAREQAHFIRVEGNEWDTLGRELELENA
>Mature_277_residues
PSPNETPSFSHLRDKALQLRRNMLIQAAGKGQGYLGQGFGLAEFFAALYFHELNFDPKYPQAPDRDRFYMSTGHYSIALW
AVFAEIGLIPKDTLTTYGADESPLEMSTIEGHVPGVEMTGGSLGHGLGIAAGAALGLRIQNNPARVFVEISDGELQEGST
WEAATAASAFRLDNLVCFNDCNGIQADGALVVPMEPVADKWRAFGWDVQEVDGNALEELIGAMHWARARRGKPKVIVMRT
RPGRGIARLEAREQAHFIRVEGNEWDTLGRELELENA

Specific function: Unknown

COG id: COG3959

COG function: function code G; Transketolase, N-terminal subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family [H]

Homologues:

Organism=Homo sapiens, GI133778974, Length=267, Percent_Identity=36.3295880149813, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI205277463, Length=252, Percent_Identity=36.9047619047619, Blast_Score=134, Evalue=7e-32,
Organism=Homo sapiens, GI4507521, Length=252, Percent_Identity=36.9047619047619, Blast_Score=134, Evalue=7e-32,
Organism=Homo sapiens, GI225637459, Length=250, Percent_Identity=29.2, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI225637461, Length=219, Percent_Identity=30.5936073059361, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI225637463, Length=217, Percent_Identity=30.4147465437788, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1788808, Length=257, Percent_Identity=30.3501945525292, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI48994911, Length=239, Percent_Identity=29.2887029288703, Blast_Score=90, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17539652, Length=252, Percent_Identity=33.7301587301587, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6325331, Length=263, Percent_Identity=30.7984790874525, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319593, Length=254, Percent_Identity=29.9212598425197, Blast_Score=91, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45551847, Length=259, Percent_Identity=32.046332046332, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI45550715, Length=259, Percent_Identity=32.046332046332, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24666278, Length=257, Percent_Identity=31.1284046692607, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24645119, Length=219, Percent_Identity=33.7899543378995, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005474 [H]

Pfam domain/function: PF00456 Transketolase_N [H]

EC number: 2.2.1.1 [C]

Molecular weight: Translated: 30514; Mature: 30383

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPNETPSFSHLRDKALQLRRNMLIQAAGKGQGYLGQGFGLAEFFAALYFHELNFDPKY
CCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
PQAPDRDRFYMSTGHYSIALWAVFAEIGLIPKDTLTTYGADESPLEMSTIEGHVPGVEMT
CCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHEECCCCCCCCEEEEECCCCCCEEEC
GGSLGHGLGIAAGAALGLRIQNNPARVFVEISDGELQEGSTWEAATAASAFRLDNLVCFN
CCCCCCCHHHHHHCEEEEEEECCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCEEEEC
DCNGIQADGALVVPMEPVADKWRAFGWDVQEVDGNALEELIGAMHWARARRGKPKVIVMR
CCCCCCCCCEEEEECCHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
TRPGRGIARLEAREQAHFIRVEGNEWDTLGRELELENA
CCCCCCHHHHHHCCCEEEEEEECCCHHHCCCEEEECCC
>Mature Secondary Structure 
PSPNETPSFSHLRDKALQLRRNMLIQAAGKGQGYLGQGFGLAEFFAALYFHELNFDPKY
CCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
PQAPDRDRFYMSTGHYSIALWAVFAEIGLIPKDTLTTYGADESPLEMSTIEGHVPGVEMT
CCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHEECCCCCCCCEEEEECCCCCCEEEC
GGSLGHGLGIAAGAALGLRIQNNPARVFVEISDGELQEGSTWEAATAASAFRLDNLVCFN
CCCCCCCHHHHHHCEEEEEEECCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCEEEEC
DCNGIQADGALVVPMEPVADKWRAFGWDVQEVDGNALEELIGAMHWARARRGKPKVIVMR
CCCCCCCCCEEEEECCHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
TRPGRGIARLEAREQAHFIRVEGNEWDTLGRELELENA
CCCCCCHHHHHHCCCEEEEEEECCCHHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Thiamine diphosphate, mono-or triphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Erythrose 4-phosphate; D-Xylulose 5-phosphate [C]

Specific reaction: D-Erythrose 4-phosphate + D-Xylulose 5-phosphate <==> D-Fructose 6-phosphate + Glyceraldehyde 3-phosphate [C]

General reaction: Keto group transfer [C]

Inhibitor: EDTA; Oxythiamine diphosphate; Phosphate; Rabbit Antibodies; Sulfate [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]