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The map label for this gene is pbpD [H]

Identifier: 154245597

GI number: 154245597

Start: 1871168

End: 1873474

Strand: Reverse

Name: pbpD [H]

Synonym: Xaut_1653

Alternate gene names: 154245597

Gene position: 1873474-1871168 (Counterclockwise)

Preceding gene: 154245598

Following gene: 154245595

Centisome position: 35.29

GC content: 68.36

Gene sequence:

>2307_bases
GTGACCGAGCGCGAACCGACCGACCCCAAGGCCCGCGACGAGAACGCCCGCGACGACAAGCGCCCCGACGACCTGCGCCC
CGATTCGAGGCGTCCCGCCCCGGAGCGCTCCTCGTTCGGCACGCGCCTGCGCGGGCTGATGCTCGATTTCGACGCCCTGC
TCGACAGCGGGCTGTTCAGCCTCGGCGCGCGCACCCGCCGGGCGGTTTCCGCCTATGCGGCCTTCACCGAGCGGTTCAAC
CTCACCGGCGTGTCGCGCTTCGCCGCCGGCATGGCCTCCGAAGGCCTGACCCTGACGCTGGCCGGCGCGCTGGTGATGCT
GGCCCTGGCCATCCCGGCCTTCCGCGAAACCTCCGACGACTGGCTCAAGCGCACCGAACTCGCCGTCACCTTCCTCGATC
GCTACGGCAATACCATCGGCGAGCGGGGCGTGAAGCAGAACGACACGGTGCCGCTGGAGGACATCCCCGACAACCTCATC
AAGGCGACGCTCGCCACCGAGGACCGGCGCTTCTACGAGCACTGGGGCATCGATGTGGCCGGCACGTTCCGCGCCGTGGT
CACCAATGCGCGGGCCGGCGGCGTGCGGCAGGGCGGCTCCTCGCTCTCCCAGCAGTTGGCCAAGAACCTGTTCCTGTCCA
ACGAGCGCACCATCGAGCGCAAGATCAAGGAAGCCTTCCTTGCCATCTGGCTGGAGGCGCGCCTCACCAAGAGCCAGATC
CTGAAGCTCTACCTCGACCGCGCCTATCTCGGCGGCGGCGCTTACGGGGTGGATGCGGCGGCGCGCTATTATTTCGGCAA
GTCGGTGCGCGAGGTGAACCTCGCCGAGGCCGCCATGATGGCCGGCCTGTTCAAGGCGCCGTCCAAATACGCCCCCCACG
TGAACCTGCCCGCCGCCCGTGCCCGCGCCTCCACCGTGCTCGACAATCTGGTGGATGCCGGCTTCATGACCGAGGGCCAG
GTGTTCGGCGCCCGCCGCAACCCGGCCACCCCGGTGGACCGTCGCGACGACGACGTGCCGGATTTCTATCTCGATTACGC
CTTCGGCGAGGTGAAGAAGCTGGTGGACCAGCTGCCGCCCACGGTGGTGGAGCGCAACTTCATCGTGCGCACCGGCCTCG
ACCCCAACATCCAGAAGGTGGCGGACGCGGCGCTGGAGACCTCGCTCGCCGAGTTCGGCCGCGATTACAAGGTGAAGCAG
GGCGGCATCGTGGTGATGGAGCCCAACGGCACCCTGCGCACCATGGTGGGCGGGCGCGACTATGGCGAAAGCCAGTTCAA
CCGCGCCACCGATGCCCTGCGCCAGCCCGGCTCCTCCTTCAAGCCCTTCGTCTACACGGTGGCGCTGATGAACGGCTTCA
AGCCGTCCACCATCGTGCAGGACGCGCCCATCTGCCTCGGCAACTGGTGCCCGCAGAATTACGGCCGCTCCTACATGGGC
TCCATCACCCTGAAGACGGCGCTCCAGCATTCGCTGAACACGGTGGCGGTGCGCCTCGCGGAGAAGTTCGGCCGCAAGAA
GATCGTGGATCTCGCCCACCAGATGGGCATCCGCACCCAGCTGCCCATCAGCGCGCCGCTGCCGCTGGGCGCCGCCGAGG
TGACCCTGTTCGACATGACCACCGCCTATTCGGTGTTCGCCAACGGCGGCAAGAGCGTCTATCCCCACGCGGTGGTGGAG
ATGCGCGTCGGCAATGGCGAGGTGGTGTGGCGCTTCGACCGCGACGCGCCCAAGAGCGTGCAGATCATCCCCACCCAGCA
GCTCTATGACATCAACGACATGCTGAACAACGTGGTGGAGAACGGCACCGGCCGCCGCGCCCGCCTCGTCAATTCCAAGG
TGGCGGGCAAGACCGGCACCACCAATGCCTATCGCGATGCCTGGTTCATGGGCTTCACCGGCAATTACGTGGCCGGGGTG
TGGTTCGGCAACGACGACTATGCCGTGATGAACAAGATGACCGGCGGCACCCTGCCCGCCATGACCTGGCAGAAGGTGAT
GGCCTATGCCCACCAGGGCATCGAGATCAAGCCGATCCCCGGCGTCACCTCGCCGATCCCGAAGCTGGACGAGACACCCG
CGCCCATGAACGTGCCGGGCCTCGACACCGAGCGGCCGGTCACCCTCTCCCCGCGCGCGGCCGAACGGCTCGCGGCGCTG
GAGCGCATGCTGAAGGAGGCCGAGGTCCGCCTCACCGATGCCACCCCGGCCGCCCCGCCCCAACCCGCCGCCCGTTCCGC
CGGCCCTGCAGTTCCGAGTGCGCCCCCCGGTGCGGCCCCGATGCCCGCACCGGTGCAGCGCAACTGA

Upstream 100 bases:

>100_bases
TTTCGGCGACGCGTTATAGAGTGGGCCCAAGCAACACAGACATGAGCGGCGATGCGCGGGCCCGGGGGCGCCGCCGGAGC
CGCTGAACCGAGCCCGAACC

Downstream 100 bases:

>100_bases
GCACCGGCAGAACGGCGCATCGCGTCCCGGACCAGCGACGGCGCAGCCGGAGCGCCGAGCCGGCATGGCAGAAACTCCTG
CGCAGCAGGCAATCGGGATG

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP 4 [H]

Number of amino acids: Translated: 768; Mature: 767

Protein sequence:

>768_residues
MTEREPTDPKARDENARDDKRPDDLRPDSRRPAPERSSFGTRLRGLMLDFDALLDSGLFSLGARTRRAVSAYAAFTERFN
LTGVSRFAAGMASEGLTLTLAGALVMLALAIPAFRETSDDWLKRTELAVTFLDRYGNTIGERGVKQNDTVPLEDIPDNLI
KATLATEDRRFYEHWGIDVAGTFRAVVTNARAGGVRQGGSSLSQQLAKNLFLSNERTIERKIKEAFLAIWLEARLTKSQI
LKLYLDRAYLGGGAYGVDAAARYYFGKSVREVNLAEAAMMAGLFKAPSKYAPHVNLPAARARASTVLDNLVDAGFMTEGQ
VFGARRNPATPVDRRDDDVPDFYLDYAFGEVKKLVDQLPPTVVERNFIVRTGLDPNIQKVADAALETSLAEFGRDYKVKQ
GGIVVMEPNGTLRTMVGGRDYGESQFNRATDALRQPGSSFKPFVYTVALMNGFKPSTIVQDAPICLGNWCPQNYGRSYMG
SITLKTALQHSLNTVAVRLAEKFGRKKIVDLAHQMGIRTQLPISAPLPLGAAEVTLFDMTTAYSVFANGGKSVYPHAVVE
MRVGNGEVVWRFDRDAPKSVQIIPTQQLYDINDMLNNVVENGTGRRARLVNSKVAGKTGTTNAYRDAWFMGFTGNYVAGV
WFGNDDYAVMNKMTGGTLPAMTWQKVMAYAHQGIEIKPIPGVTSPIPKLDETPAPMNVPGLDTERPVTLSPRAAERLAAL
ERMLKEAEVRLTDATPAAPPQPAARSAGPAVPSAPPGAAPMPAPVQRN

Sequences:

>Translated_768_residues
MTEREPTDPKARDENARDDKRPDDLRPDSRRPAPERSSFGTRLRGLMLDFDALLDSGLFSLGARTRRAVSAYAAFTERFN
LTGVSRFAAGMASEGLTLTLAGALVMLALAIPAFRETSDDWLKRTELAVTFLDRYGNTIGERGVKQNDTVPLEDIPDNLI
KATLATEDRRFYEHWGIDVAGTFRAVVTNARAGGVRQGGSSLSQQLAKNLFLSNERTIERKIKEAFLAIWLEARLTKSQI
LKLYLDRAYLGGGAYGVDAAARYYFGKSVREVNLAEAAMMAGLFKAPSKYAPHVNLPAARARASTVLDNLVDAGFMTEGQ
VFGARRNPATPVDRRDDDVPDFYLDYAFGEVKKLVDQLPPTVVERNFIVRTGLDPNIQKVADAALETSLAEFGRDYKVKQ
GGIVVMEPNGTLRTMVGGRDYGESQFNRATDALRQPGSSFKPFVYTVALMNGFKPSTIVQDAPICLGNWCPQNYGRSYMG
SITLKTALQHSLNTVAVRLAEKFGRKKIVDLAHQMGIRTQLPISAPLPLGAAEVTLFDMTTAYSVFANGGKSVYPHAVVE
MRVGNGEVVWRFDRDAPKSVQIIPTQQLYDINDMLNNVVENGTGRRARLVNSKVAGKTGTTNAYRDAWFMGFTGNYVAGV
WFGNDDYAVMNKMTGGTLPAMTWQKVMAYAHQGIEIKPIPGVTSPIPKLDETPAPMNVPGLDTERPVTLSPRAAERLAAL
ERMLKEAEVRLTDATPAAPPQPAARSAGPAVPSAPPGAAPMPAPVQRN
>Mature_767_residues
TEREPTDPKARDENARDDKRPDDLRPDSRRPAPERSSFGTRLRGLMLDFDALLDSGLFSLGARTRRAVSAYAAFTERFNL
TGVSRFAAGMASEGLTLTLAGALVMLALAIPAFRETSDDWLKRTELAVTFLDRYGNTIGERGVKQNDTVPLEDIPDNLIK
ATLATEDRRFYEHWGIDVAGTFRAVVTNARAGGVRQGGSSLSQQLAKNLFLSNERTIERKIKEAFLAIWLEARLTKSQIL
KLYLDRAYLGGGAYGVDAAARYYFGKSVREVNLAEAAMMAGLFKAPSKYAPHVNLPAARARASTVLDNLVDAGFMTEGQV
FGARRNPATPVDRRDDDVPDFYLDYAFGEVKKLVDQLPPTVVERNFIVRTGLDPNIQKVADAALETSLAEFGRDYKVKQG
GIVVMEPNGTLRTMVGGRDYGESQFNRATDALRQPGSSFKPFVYTVALMNGFKPSTIVQDAPICLGNWCPQNYGRSYMGS
ITLKTALQHSLNTVAVRLAEKFGRKKIVDLAHQMGIRTQLPISAPLPLGAAEVTLFDMTTAYSVFANGGKSVYPHAVVEM
RVGNGEVVWRFDRDAPKSVQIIPTQQLYDINDMLNNVVENGTGRRARLVNSKVAGKTGTTNAYRDAWFMGFTGNYVAGVW
FGNDDYAVMNKMTGGTLPAMTWQKVMAYAHQGIEIKPIPGVTSPIPKLDETPAPMNVPGLDTERPVTLSPRAAERLAALE
RMLKEAEVRLTDATPAAPPQPAARSAGPAVPSAPPGAAPMPAPVQRN

Specific function: Cell Wall Formation. Synthesis Of Cross-Linked Peptidoglycan From The Lipid Intermediates. The Enzyme Has A Penicillin-Insensitive Transglycosylase N-Terminal Domain (Formation Of Linear Glycan Strands) And A Penicillin-Sensitive Transpeptidase C-Terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=547, Percent_Identity=33.0895795246801, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1788867, Length=516, Percent_Identity=30.2325581395349, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI87082258, Length=279, Percent_Identity=38.7096774193548, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1789601, Length=183, Percent_Identity=36.0655737704918, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 83935; Mature: 83803

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEREPTDPKARDENARDDKRPDDLRPDSRRPAPERSSFGTRLRGLMLDFDALLDSGLFS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGARTRRAVSAYAAFTERFNLTGVSRFAAGMASEGLTLTLAGALVMLALAIPAFRETSDD
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCHH
WLKRTELAVTFLDRYGNTIGERGVKQNDTVPLEDIPDNLIKATLATEDRRFYEHWGIDVA
HHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCHH
GTFRAVVTNARAGGVRQGGSSLSQQLAKNLFLSNERTIERKIKEAFLAIWLEARLTKSQI
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LKLYLDRAYLGGGAYGVDAAARYYFGKSVREVNLAEAAMMAGLFKAPSKYAPHVNLPAAR
HHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
ARASTVLDNLVDAGFMTEGQVFGARRNPATPVDRRDDDVPDFYLDYAFGEVKKLVDQLPP
HHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCH
TVVERNFIVRTGLDPNIQKVADAALETSLAEFGRDYKVKQGGIVVMEPNGTLRTMVGGRD
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCEEEECCCCCEEEEECCCC
YGESQFNRATDALRQPGSSFKPFVYTVALMNGFKPSTIVQDAPICLGNWCPQNYGRSYMG
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCHHHCCHHHH
SITLKTALQHSLNTVAVRLAEKFGRKKIVDLAHQMGIRTQLPISAPLPLGAAEVTLFDMT
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHH
TAYSVFANGGKSVYPHAVVEMRVGNGEVVWRFDRDAPKSVQIIPTQQLYDINDMLNNVVE
HHHHHHHCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHH
NGTGRRARLVNSKVAGKTGTTNAYRDAWFMGFTGNYVAGVWFGNDDYAVMNKMTGGTLPA
CCCCCEEEHHCCHHCCCCCCCCCHHCEEEECCCCCEEEEEEECCCCHHHHHHCCCCCCCH
MTWQKVMAYAHQGIEIKPIPGVTSPIPKLDETPAPMNVPGLDTERPVTLSPRAAERLAAL
HHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHH
ERMLKEAEVRLTDATPAAPPQPAARSAGPAVPSAPPGAAPMPAPVQRN
HHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEREPTDPKARDENARDDKRPDDLRPDSRRPAPERSSFGTRLRGLMLDFDALLDSGLFS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGARTRRAVSAYAAFTERFNLTGVSRFAAGMASEGLTLTLAGALVMLALAIPAFRETSDD
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCHH
WLKRTELAVTFLDRYGNTIGERGVKQNDTVPLEDIPDNLIKATLATEDRRFYEHWGIDVA
HHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCHH
GTFRAVVTNARAGGVRQGGSSLSQQLAKNLFLSNERTIERKIKEAFLAIWLEARLTKSQI
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LKLYLDRAYLGGGAYGVDAAARYYFGKSVREVNLAEAAMMAGLFKAPSKYAPHVNLPAAR
HHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
ARASTVLDNLVDAGFMTEGQVFGARRNPATPVDRRDDDVPDFYLDYAFGEVKKLVDQLPP
HHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCH
TVVERNFIVRTGLDPNIQKVADAALETSLAEFGRDYKVKQGGIVVMEPNGTLRTMVGGRD
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCEEEECCCCCEEEEECCCC
YGESQFNRATDALRQPGSSFKPFVYTVALMNGFKPSTIVQDAPICLGNWCPQNYGRSYMG
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCHHHCCHHHH
SITLKTALQHSLNTVAVRLAEKFGRKKIVDLAHQMGIRTQLPISAPLPLGAAEVTLFDMT
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHH
TAYSVFANGGKSVYPHAVVEMRVGNGEVVWRFDRDAPKSVQIIPTQQLYDINDMLNNVVE
HHHHHHHCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHH
NGTGRRARLVNSKVAGKTGTTNAYRDAWFMGFTGNYVAGVWFGNDDYAVMNKMTGGTLPA
CCCCCEEEHHCCHHCCCCCCCCCHHCEEEECCCCCEEEEEEECCCCHHHHHHCCCCCCCH
MTWQKVMAYAHQGIEIKPIPGVTSPIPKLDETPAPMNVPGLDTERPVTLSPRAAERLAAL
HHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHH
ERMLKEAEVRLTDATPAAPPQPAARSAGPAVPSAPPGAAPMPAPVQRN
HHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961491; 9274030; 9384377 [H]