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The map label for this gene is livJ [C]

Identifier: 154245565

GI number: 154245565

Start: 1834257

End: 1835483

Strand: Reverse

Name: livJ [C]

Synonym: Xaut_1620

Alternate gene names: 154245565

Gene position: 1835483-1834257 (Counterclockwise)

Preceding gene: 154245570

Following gene: 154245564

Centisome position: 34.57

GC content: 66.75

Gene sequence:

>1227_bases
ATGAAGAGTTTCGCGCGGGCGGCGTTGGCCGCCGCACTGCTTGTTGCCGCCTCCCCCTTCGCGGGACCCGCGGCGGCCCA
GGGCACGGTCAAGTTCGGCGCCCTCTATCCCTTCTCCGGCGGCCTCGCCCTTCTGGGCGACGAGAGCTTCCGCGGCTTCG
AGCTGGCGGTGGAGGAGCGCAACGCGGCCGGCGGCATCGACGGCAAGAAGATCGAGTTCATCAAGGGCGACGCGGTCGAT
CCCAACCAGGCCGTTGGCGAGGCCCGGCGCCTGACCTCGGTGGACAAGGTCTCGGCCATCTTCGGCACCTACTCCTCGTC
CCTGTCGCTGGCAGCGACGCAGGTGACTGAGCTTGCCGGCGTGCCCTATTTCGAGCTTGGCGCGGTGTCCGATCCCATCA
CTGAGCGCGGCTTCAAATACGTGTTCCGCACCAACGCCACGGCCCGTGACTTCGCCAACCGCATGGTGGATTCGGTGATC
GAGGCGGTGGCCCCGGCCCTCGGCGTAAAGGCGGCTGACCTCAAGGTCGGCATCATCCACGAGGACAGCCTCTACGGCCA
GACGGTCTCCGGCTATCAGAAGACCCGCGCCAAGGAGAAGGGCCTCAACGTGGTCGAGGTCCTGCCCTATTCGGCCAAGT
CCGTCGACCTCACCCCGGTCATCCTGCGCCTCAAGTCCGCAGGCGTGGACGTAGTGCTGCAGACCTCCTACCAGAACGAC
ACCGTGCTGTTCTTCCGCCAAATGAAGGACCAGGCCTACAAGCCCAAGGCGGTGATGGGCGCCGGCGGCGGCTATTCCAC
CCGTGACACCATGACTGCGGTGGGCGCGGAGAACATGGAAGGCGCCCTCGACGTGGACTTCACCCAGTTCAACACGAACC
CCAAGGCCACCCCCGGCCTCGCGGAATTCGTGACCAAGTACAAGGCGAAGTACGGCATCGATCCGCGCTCCGGCCATTCG
CTGGCCAACTACATGGGCGCCAAGGTGTTCTTCGACGCCATCGCCAGCGCCAAGAGCACCGACGCCGACAAGGTGCGCGC
CGCCGTCCTGAAGGTGGACATCCCCGTGGGCGAGACCGCCACCGGCTGGGGCGCCAAGTTCTCCGAGCAGGGCCAGAACA
CCCGCGCCCAGCCGTTCCTCATGCAGTGGCAGGGCGGCAACCTCGTCACCGTGTTCCCCGAGTCGGCCGCGGTCGCCAAG
CTGAAGACCGGCGTCGGCGCCAACTGA

Upstream 100 bases:

>100_bases
GCGGCAATGTCCGACCGTCCGCCATCAGGCGCTTAAAAATCGTTCCCGATTAACGGGTTACAGGGGCCGGCCGCAGACGC
GCCGGACGAGGGAGGAGACA

Downstream 100 bases:

>100_bases
CCCGTCCCGACTGCCCCGCGGCGCCCGGAAGGGCCGCCGCCCGGCCCCCGCGCGATCTGGCCTCGCGCGGGGGCCGTAGT
TCCCGACAATTCGCTTCCCG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MKSFARAALAAALLVAASPFAGPAAAQGTVKFGALYPFSGGLALLGDESFRGFELAVEERNAAGGIDGKKIEFIKGDAVD
PNQAVGEARRLTSVDKVSAIFGTYSSSLSLAATQVTELAGVPYFELGAVSDPITERGFKYVFRTNATARDFANRMVDSVI
EAVAPALGVKAADLKVGIIHEDSLYGQTVSGYQKTRAKEKGLNVVEVLPYSAKSVDLTPVILRLKSAGVDVVLQTSYQND
TVLFFRQMKDQAYKPKAVMGAGGGYSTRDTMTAVGAENMEGALDVDFTQFNTNPKATPGLAEFVTKYKAKYGIDPRSGHS
LANYMGAKVFFDAIASAKSTDADKVRAAVLKVDIPVGETATGWGAKFSEQGQNTRAQPFLMQWQGGNLVTVFPESAAVAK
LKTGVGAN

Sequences:

>Translated_408_residues
MKSFARAALAAALLVAASPFAGPAAAQGTVKFGALYPFSGGLALLGDESFRGFELAVEERNAAGGIDGKKIEFIKGDAVD
PNQAVGEARRLTSVDKVSAIFGTYSSSLSLAATQVTELAGVPYFELGAVSDPITERGFKYVFRTNATARDFANRMVDSVI
EAVAPALGVKAADLKVGIIHEDSLYGQTVSGYQKTRAKEKGLNVVEVLPYSAKSVDLTPVILRLKSAGVDVVLQTSYQND
TVLFFRQMKDQAYKPKAVMGAGGGYSTRDTMTAVGAENMEGALDVDFTQFNTNPKATPGLAEFVTKYKAKYGIDPRSGHS
LANYMGAKVFFDAIASAKSTDADKVRAAVLKVDIPVGETATGWGAKFSEQGQNTRAQPFLMQWQGGNLVTVFPESAAVAK
LKTGVGAN
>Mature_408_residues
MKSFARAALAAALLVAASPFAGPAAAQGTVKFGALYPFSGGLALLGDESFRGFELAVEERNAAGGIDGKKIEFIKGDAVD
PNQAVGEARRLTSVDKVSAIFGTYSSSLSLAATQVTELAGVPYFELGAVSDPITERGFKYVFRTNATARDFANRMVDSVI
EAVAPALGVKAADLKVGIIHEDSLYGQTVSGYQKTRAKEKGLNVVEVLPYSAKSVDLTPVILRLKSAGVDVVLQTSYQND
TVLFFRQMKDQAYKPKAVMGAGGGYSTRDTMTAVGAENMEGALDVDFTQFNTNPKATPGLAEFVTKYKAKYGIDPRSGHS
LANYMGAKVFFDAIASAKSTDADKVRAAVLKVDIPVGETATGWGAKFSEQGQNTRAQPFLMQWQGGNLVTVFPESAAVAK
LKTGVGAN

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=299, Percent_Identity=26.7558528428094, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789867, Length=280, Percent_Identity=26.4285714285714, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 43077; Mature: 43077

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFARAALAAALLVAASPFAGPAAAQGTVKFGALYPFSGGLALLGDESFRGFELAVEER
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCEEEEEECC
NAAGGIDGKKIEFIKGDAVDPNQAVGEARRLTSVDKVSAIFGTYSSSLSLAATQVTELAG
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
VPYFELGAVSDPITERGFKYVFRTNATARDFANRMVDSVIEAVAPALGVKAADLKVGIIH
CCCEECCCCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCEECCEEEEEEE
EDSLYGQTVSGYQKTRAKEKGLNVVEVLPYSAKSVDLTPVILRLKSAGVDVVLQTSYQND
CCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHEEHHCCCCEEEEEECCCCC
TVLFFRQMKDQAYKPKAVMGAGGGYSTRDTMTAVGAENMEGALDVDFTQFNTNPKATPGL
EEEEEEHHHHHCCCCCEEEECCCCCCCCHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCH
AEFVTKYKAKYGIDPRSGHSLANYMGAKVFFDAIASAKSTDADKVRAAVLKVDIPVGETA
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEEEECCCCCCC
TGWGAKFSEQGQNTRAQPFLMQWQGGNLVTVFPESAAVAKLKTGVGAN
CCCCCCCHHCCCCCCCCCEEEEECCCCEEEECCCCHHHHHHHCCCCCC
>Mature Secondary Structure
MKSFARAALAAALLVAASPFAGPAAAQGTVKFGALYPFSGGLALLGDESFRGFELAVEER
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCEEEEEECC
NAAGGIDGKKIEFIKGDAVDPNQAVGEARRLTSVDKVSAIFGTYSSSLSLAATQVTELAG
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
VPYFELGAVSDPITERGFKYVFRTNATARDFANRMVDSVIEAVAPALGVKAADLKVGIIH
CCCEECCCCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCEECCEEEEEEE
EDSLYGQTVSGYQKTRAKEKGLNVVEVLPYSAKSVDLTPVILRLKSAGVDVVLQTSYQND
CCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHEEHHCCCCEEEEEECCCCC
TVLFFRQMKDQAYKPKAVMGAGGGYSTRDTMTAVGAENMEGALDVDFTQFNTNPKATPGL
EEEEEEHHHHHCCCCCEEEECCCCCCCCHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCH
AEFVTKYKAKYGIDPRSGHSLANYMGAKVFFDAIASAKSTDADKVRAAVLKVDIPVGETA
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEEEECCCCCCC
TGWGAKFSEQGQNTRAQPFLMQWQGGNLVTVFPESAAVAKLKTGVGAN
CCCCCCCHHCCCCCCCCCEEEEECCCCEEEECCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA