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The map label for this gene is nitA [H]

Identifier: 154245513

GI number: 154245513

Start: 1777715

End: 1778641

Strand: Direct

Name: nitA [H]

Synonym: Xaut_1567

Alternate gene names: 154245513

Gene position: 1777715-1778641 (Clockwise)

Preceding gene: 154245512

Following gene: 154245514

Centisome position: 33.49

GC content: 65.48

Gene sequence:

>927_bases
ATGAAAGTGAAAGCCGGCGTCGCCCAGGTGGGCGCGGTGCCCTTCGATGTGGAGGCGTCCGTCGCCAAGGCCGAGGCGTG
GATCGCCAAGGCGGGGGCGGAAGGCTGCGAGATCGTGGTGTTTCCCGAGGCCTTCCTCTCGGTCTATCCGAAGGGCTCGG
ACTTCGACATCGCCATCGGCGTGCGCACCGTGCGCGGGCGCGACGATTTCCGCCGCTATCTCGATGCCGCCATCACCGTG
CCGGGACCTGAGACGGACCGCATCGGCAAGGCGGCGGGCGAGGCCAAGACCTATGTGGTCATCGGCGTGATGGAGCGTGA
ACTCGGCACCATGTATTGCACGGTGCTCTATTTCGGCCCGGACGGGTCCCTGCTGGGCAAGCACCGCAAGCTGATGCCCA
CCGCCGGCGAGCGTCTCGCCTGGGGCTTCGGCGACGGCTCGACCCTGCCGGTGTTCGACACGCCCATCGGCAAGATCGGC
GCGGTGATCTGCTGGGAAAACTACATGCCCATGCTGCGCATGACCATGTACGCCAAGGGTGTCTCGCTCTATTGCGCCCC
CACCGCCGACGATCGTGAGACCTGGCTGCCCACCATGCGCCACATCGCGCTGGAAGGCCGCTGCTTCGTGCTCACCACCT
GCCAGGTGGTCAAGCGCGGTGATTTCCCGGACGACTACCGTTGCTCCATCACCGACGATCCCGAGGCGATCGTGATGCAC
GGCGGGGCGGCCATCATCGACCCCCTCGGCAAGGTGCTGGCCGGCCCGGTCTTCGATCAGGAAACCCTCTTGACCGCCGA
GCTCGACATGGACGATCTGGGCCGCGCCAAGTTCGACTTCGACGTGGCCGGCAATTATGCCCGGCCGGACGTTTTCAACC
TGACGGTCAATGAAGCCCCGCAGCAGGCTGTTCGTTTGAAAGCTTGA

Upstream 100 bases:

>100_bases
TGGTGCTTTCGGGCACCGCGCAGGCCCTCTCCGACGATCCGGGCGTGCGTCGCGCCTATCTCGGCGTGTGACGCCCCATC
AAATCCCAGGGAGAAGAACG

Downstream 100 bases:

>100_bases
AGACCTCACACGGCGGGGCATGCAATCCGCCGTGAATTCCGCCATTCTCACGGCGCGGCTCACCTGCGCCGCGCCGTGAG
ACCAACACGTCATGGATATG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKVKAGVAQVGAVPFDVEASVAKAEAWIAKAGAEGCEIVVFPEAFLSVYPKGSDFDIAIGVRTVRGRDDFRRYLDAAITV
PGPETDRIGKAAGEAKTYVVIGVMERELGTMYCTVLYFGPDGSLLGKHRKLMPTAGERLAWGFGDGSTLPVFDTPIGKIG
AVICWENYMPMLRMTMYAKGVSLYCAPTADDRETWLPTMRHIALEGRCFVLTTCQVVKRGDFPDDYRCSITDDPEAIVMH
GGAAIIDPLGKVLAGPVFDQETLLTAELDMDDLGRAKFDFDVAGNYARPDVFNLTVNEAPQQAVRLKA

Sequences:

>Translated_308_residues
MKVKAGVAQVGAVPFDVEASVAKAEAWIAKAGAEGCEIVVFPEAFLSVYPKGSDFDIAIGVRTVRGRDDFRRYLDAAITV
PGPETDRIGKAAGEAKTYVVIGVMERELGTMYCTVLYFGPDGSLLGKHRKLMPTAGERLAWGFGDGSTLPVFDTPIGKIG
AVICWENYMPMLRMTMYAKGVSLYCAPTADDRETWLPTMRHIALEGRCFVLTTCQVVKRGDFPDDYRCSITDDPEAIVMH
GGAAIIDPLGKVLAGPVFDQETLLTAELDMDDLGRAKFDFDVAGNYARPDVFNLTVNEAPQQAVRLKA
>Mature_308_residues
MKVKAGVAQVGAVPFDVEASVAKAEAWIAKAGAEGCEIVVFPEAFLSVYPKGSDFDIAIGVRTVRGRDDFRRYLDAAITV
PGPETDRIGKAAGEAKTYVVIGVMERELGTMYCTVLYFGPDGSLLGKHRKLMPTAGERLAWGFGDGSTLPVFDTPIGKIG
AVICWENYMPMLRMTMYAKGVSLYCAPTADDRETWLPTMRHIALEGRCFVLTTCQVVKRGDFPDDYRCSITDDPEAIVMH
GGAAIIDPLGKVLAGPVFDQETLLTAELDMDDLGRAKFDFDVAGNYARPDVFNLTVNEAPQQAVRLKA

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=281, Percent_Identity=25.2669039145907, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17557111, Length=301, Percent_Identity=49.1694352159468, Blast_Score=320, Evalue=4e-88,
Organism=Saccharomyces cerevisiae, GI6322027, Length=188, Percent_Identity=45.7446808510638, Blast_Score=178, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6322026, Length=110, Percent_Identity=41.8181818181818, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 33475; Mature: 33475

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00921 NITRIL_CHT_2 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKAGVAQVGAVPFDVEASVAKAEAWIAKAGAEGCEIVVFPEAFLSVYPKGSDFDIAIG
CCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHHEECCCCCCEEEEEE
VRTVRGRDDFRRYLDAAITVPGPETDRIGKAAGEAKTYVVIGVMERELGTMYCTVLYFGP
EEEECCHHHHHHHHHHEEECCCCCCCHHHHCCCCCEEEEEEEEEECCCCEEEEEEEEECC
DGSLLGKHRKLMPTAGERLAWGFGDGSTLPVFDTPIGKIGAVICWENYMPMLRMTMYAKG
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCEECCCHHHHEEEEEECCCHHHHHHHHHHCC
VSLYCAPTADDRETWLPTMRHIALEGRCFVLTTCQVVKRGDFPDDYRCSITDDPEAIVMH
EEEEEECCCCCCHHHHHHHHHEEECCCEEEEEEEHHHHCCCCCCCCEEEECCCCCEEEEE
GGAAIIDPLGKVLAGPVFDQETLLTAELDMDDLGRAKFDFDVAGNYARPDVFNLTVNEAP
CCCHHHHHHHHHHCCCCCCCCEEEEEECCHHHCCCCEEEEEECCCCCCCEEEEEEECCCC
QQAVRLKA
CCHHEECC
>Mature Secondary Structure
MKVKAGVAQVGAVPFDVEASVAKAEAWIAKAGAEGCEIVVFPEAFLSVYPKGSDFDIAIG
CCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHHEECCCCCCEEEEEE
VRTVRGRDDFRRYLDAAITVPGPETDRIGKAAGEAKTYVVIGVMERELGTMYCTVLYFGP
EEEECCHHHHHHHHHHEEECCCCCCCHHHHCCCCCEEEEEEEEEECCCCEEEEEEEEECC
DGSLLGKHRKLMPTAGERLAWGFGDGSTLPVFDTPIGKIGAVICWENYMPMLRMTMYAKG
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCEECCCHHHHEEEEEECCCHHHHHHHHHHCC
VSLYCAPTADDRETWLPTMRHIALEGRCFVLTTCQVVKRGDFPDDYRCSITDDPEAIVMH
EEEEEECCCCCCHHHHHHHHHEEECCCEEEEEEEHHHHCCCCCCCCEEEECCCCCEEEEE
GGAAIIDPLGKVLAGPVFDQETLLTAELDMDDLGRAKFDFDVAGNYARPDVFNLTVNEAP
CCCHHHHHHHHHHCCCCCCCCEEEEEECCHHHCCCCEEEEEECCCCCCCEEEEEEECCCC
QQAVRLKA
CCHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]