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The map label for this gene is braE [H]

Identifier: 154245510

GI number: 154245510

Start: 1775226

End: 1776245

Strand: Direct

Name: braE [H]

Synonym: Xaut_1564

Alternate gene names: 154245510

Gene position: 1775226-1776245 (Clockwise)

Preceding gene: 154245509

Following gene: 154245511

Centisome position: 33.44

GC content: 68.04

Gene sequence:

>1020_bases
ATGCGACAGCTTTTCTCGCCGGGGCGCGTGGTGCTGCTCCTGTTCTTTGCCGCGGCGGCGTTGCTGCCGCTGGTGGTGGA
TCCGCGCGGACATCTGCTGCGCGTCGCCGCCATGGTGCTGCTGTTCGCGGCCATGGCGCAGTCGTGGAACATCGTGGGCG
GACTTGCCAACCAGATCTCGCTGGGCCATGCCGCCTTCTTCGGCCTTGGCGCCTATACGTCCACCCTGCTGTTCCTGAAG
CTCGGCGTCTCGCCCTGGCTCGGCATGTTCGTCGGGGCCGGGGTGGCGGGGGCGGCAGCCGCGCTGCTCTCCATTCCGAC
CTTCCGGCTGAAGGGGCATTACTTCGCGCTGGCCACCCTCGCCTTCGCCGAGGTGATGCGGGTGATCGCCAACAGCTGGG
CCAGCCTCACCGGTGGCCCGGTGGGCCTCTCCATCCCCTTCATGGGCACCCAGCCGGCCTATTTCGCCTTCCGCGCGGTG
TCCTCCTATTACTGGGCCATCCTCGGGCTGCTGGTGTTTGTCTGCCTCGTCTTCCACCTGTTGGCGCGCGGCGCCATCGG
CTATCGCCTGCGGGCGGTGCGGGAGAACGAGAATGCGGCCGAGGTGGCGGGCGTCGACACGTTCCGGGTGAAACTCACCG
CCAGCGTCATATCCGCCGCGCTCACCGCCATGTGCGGCACGGTGTTTGCGCAGTTCACCTTCTTCTTCGATCCGGATTCC
ATCTTCTCGCTGGCGGGCATCTCGGTGCGCATGGCCATGATCGCCATCGTCGGCGGGCTCGGCACCTTGGGAGGGCCCCT
CATCGGCGCCCTGTTCCTGATCCCGCTGGAAGAGGTGGCGAACGCCTATCTCTCCTCCAAGGCGGCGGGCCTGTCGCAAC
TGGTCTTCGGGCTGATCCTGATCGCCGCCATCCTCATTCAGCCGCGCGGCGTCATGGCCTTCTGGCCGAAGAAGCGCCGC
GCTGCCAAGGCCGTGCCACCGGTGGTGGCGCCGGCCCAGGCCGCCGCGAAGGGGGCCTGA

Upstream 100 bases:

>100_bases
CCTCGTCTTCCCCATCCAGCTGCAGAACCTCTGCCTGTTCGTGATCTTCATCGCCGTTCTCGCCCTGCGCCCGCAGGGCC
TCATCAAGGGCGCCTGAGCC

Downstream 100 bases:

>100_bases
CAATGCACGCGCTGCTTCAAGCCGACGCCATCGCCGTTACCTTCGGCGGCCTCAAGGCGGTGGATGGCGTCTCCTTCTCC
CTGAAGGCGGGCGAGATCAT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MRQLFSPGRVVLLLFFAAAALLPLVVDPRGHLLRVAAMVLLFAAMAQSWNIVGGLANQISLGHAAFFGLGAYTSTLLFLK
LGVSPWLGMFVGAGVAGAAAALLSIPTFRLKGHYFALATLAFAEVMRVIANSWASLTGGPVGLSIPFMGTQPAYFAFRAV
SSYYWAILGLLVFVCLVFHLLARGAIGYRLRAVRENENAAEVAGVDTFRVKLTASVISAALTAMCGTVFAQFTFFFDPDS
IFSLAGISVRMAMIAIVGGLGTLGGPLIGALFLIPLEEVANAYLSSKAAGLSQLVFGLILIAAILIQPRGVMAFWPKKRR
AAKAVPPVVAPAQAAAKGA

Sequences:

>Translated_339_residues
MRQLFSPGRVVLLLFFAAAALLPLVVDPRGHLLRVAAMVLLFAAMAQSWNIVGGLANQISLGHAAFFGLGAYTSTLLFLK
LGVSPWLGMFVGAGVAGAAAALLSIPTFRLKGHYFALATLAFAEVMRVIANSWASLTGGPVGLSIPFMGTQPAYFAFRAV
SSYYWAILGLLVFVCLVFHLLARGAIGYRLRAVRENENAAEVAGVDTFRVKLTASVISAALTAMCGTVFAQFTFFFDPDS
IFSLAGISVRMAMIAIVGGLGTLGGPLIGALFLIPLEEVANAYLSSKAAGLSQLVFGLILIAAILIQPRGVMAFWPKKRR
AAKAVPPVVAPAQAAAKGA
>Mature_339_residues
MRQLFSPGRVVLLLFFAAAALLPLVVDPRGHLLRVAAMVLLFAAMAQSWNIVGGLANQISLGHAAFFGLGAYTSTLLFLK
LGVSPWLGMFVGAGVAGAAAALLSIPTFRLKGHYFALATLAFAEVMRVIANSWASLTGGPVGLSIPFMGTQPAYFAFRAV
SSYYWAILGLLVFVCLVFHLLARGAIGYRLRAVRENENAAEVAGVDTFRVKLTASVISAALTAMCGTVFAQFTFFFDPDS
IFSLAGISVRMAMIAIVGGLGTLGGPLIGALFLIPLEEVANAYLSSKAAGLSQLVFGLILIAAILIQPRGVMAFWPKKRR
AAKAVPPVVAPAQAAAKGA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=330, Percent_Identity=28.7878787878788, Blast_Score=101, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 35565; Mature: 35565

Theoretical pI: Translated: 10.92; Mature: 10.92

Prosite motif: PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQLFSPGRVVLLLFFAAAALLPLVVDPRGHLLRVAAMVLLFAAMAQSWNIVGGLANQIS
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LGHAAFFGLGAYTSTLLFLKLGVSPWLGMFVGAGVAGAAAALLSIPTFRLKGHYFALATL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCHHCCCHHHHHHHH
AFAEVMRVIANSWASLTGGPVGLSIPFMGTQPAYFAFRAVSSYYWAILGLLVFVCLVFHL
HHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LARGAIGYRLRAVRENENAAEVAGVDTFRVKLTASVISAALTAMCGTVFAQFTFFFDPDS
HHCCCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHH
IFSLAGISVRMAMIAIVGGLGTLGGPLIGALFLIPLEEVANAYLSSKAAGLSQLVFGLIL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
IAAILIQPRGVMAFWPKKRRAAKAVPPVVAPAQAAAKGA
HHHHHHCCCCCEEECCCHHHHHHCCCCCCCCHHHHCCCC
>Mature Secondary Structure
MRQLFSPGRVVLLLFFAAAALLPLVVDPRGHLLRVAAMVLLFAAMAQSWNIVGGLANQIS
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LGHAAFFGLGAYTSTLLFLKLGVSPWLGMFVGAGVAGAAAALLSIPTFRLKGHYFALATL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCHHCCCHHHHHHHH
AFAEVMRVIANSWASLTGGPVGLSIPFMGTQPAYFAFRAVSSYYWAILGLLVFVCLVFHL
HHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LARGAIGYRLRAVRENENAAEVAGVDTFRVKLTASVISAALTAMCGTVFAQFTFFFDPDS
HHCCCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHH
IFSLAGISVRMAMIAIVGGLGTLGGPLIGALFLIPLEEVANAYLSSKAAGLSQLVFGLIL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
IAAILIQPRGVMAFWPKKRRAAKAVPPVVAPAQAAAKGA
HHHHHHCCCCCEEECCCHHHHHHCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]