Click here to switch to the map view.

The map label for this gene is fixJ [H]

Identifier: 154245499

GI number: 154245499

Start: 1764913

End: 1765512

Strand: Direct

Name: fixJ [H]

Synonym: Xaut_1552

Alternate gene names: 154245499

Gene position: 1764913-1765512 (Clockwise)

Preceding gene: 154245498

Following gene: 154245500

Centisome position: 33.24

GC content: 68.67

Gene sequence:

>600_bases
ATGAAGCCCCTGCGCGTCTATATCGTGGACGATGACGCGGCGGTGCGGCAGGCGCTGTCGCTGCTGCTGTCCACCTGCGG
CATGACGGTGGAGACCTTCGCCGACCCCGAGACCTTCCTGGCCCATGTGGACACGGCGCGCCCCGGCTGTCTGCTTATCG
ATCTGCGCATGCCGCTGGTCAGCGGGCTCCAGCTCAAGGCGGCGCTGACCGCGCGCGGCGTCCATTGGCCGGCCATCATG
ATCACCGGCCATGGCGACGTGAACGCCTGCCGGCGCGCCTTCAAAGCCGGCATCGTGGATTTCCTGACCAAGCCGGTGGA
CGAGCAGGTGCTGCTGGATGCCCTCGCCGCCGCCGCCAAGGTGCTGGAGGAGGTGCTGGAACGGCAGGAGACTCATGCCC
TGCTCGCCAACCTCACGGAGCGCGAGCGGGAGGTGATGGACCTGGTGTGCCGGGGCTGGGCCAGCAAGGAGATCGCGGCA
GCGCTCGCCGTCTCGCCGCGCACCATCGATGCCCACCGCGCCAACATCGCCGAGAAGCTCGGCACCAGTTCGGTGGCGGA
CCTGGTGCGCATGTCCATTGCTGCACCGCCGTCTCGGTAG

Upstream 100 bases:

>100_bases
AGCGGGCCGGCGGCTCCATCCGTGCGGATGCCGGGGCGACCACGGGTGCGGCCTTCGTCATCATCCTGCCCGCCGCAGCG
GCGCGCATGGAGGCGGCGCA

Downstream 100 bases:

>100_bases
TCCTACCTAGCCGCCCGTGTGAAGGCGCGGATTAAGCGGCCGCCCGTCGCGCGCTAAAGCATGGGCCGTGGCGGCGCGGT
TCCGCCACCCTGATCCGCTT

Product: response regulator receiver protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MKPLRVYIVDDDAAVRQALSLLLSTCGMTVETFADPETFLAHVDTARPGCLLIDLRMPLVSGLQLKAALTARGVHWPAIM
ITGHGDVNACRRAFKAGIVDFLTKPVDEQVLLDALAAAAKVLEEVLERQETHALLANLTEREREVMDLVCRGWASKEIAA
ALAVSPRTIDAHRANIAEKLGTSSVADLVRMSIAAPPSR

Sequences:

>Translated_199_residues
MKPLRVYIVDDDAAVRQALSLLLSTCGMTVETFADPETFLAHVDTARPGCLLIDLRMPLVSGLQLKAALTARGVHWPAIM
ITGHGDVNACRRAFKAGIVDFLTKPVDEQVLLDALAAAAKVLEEVLERQETHALLANLTEREREVMDLVCRGWASKEIAA
ALAVSPRTIDAHRANIAEKLGTSSVADLVRMSIAAPPSR
>Mature_199_residues
MKPLRVYIVDDDAAVRQALSLLLSTCGMTVETFADPETFLAHVDTARPGCLLIDLRMPLVSGLQLKAALTARGVHWPAIM
ITGHGDVNACRRAFKAGIVDFLTKPVDEQVLLDALAAAAKVLEEVLERQETHALLANLTEREREVMDLVCRGWASKEIAA
ALAVSPRTIDAHRANIAEKLGTSSVADLVRMSIAAPPSR

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=132, Percent_Identity=31.8181818181818, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1790102, Length=192, Percent_Identity=31.7708333333333, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788521, Length=199, Percent_Identity=31.6582914572864, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 21501; Mature: 21501

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLRVYIVDDDAAVRQALSLLLSTCGMTVETFADPETFLAHVDTARPGCLLIDLRMPLV
CCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHCCCCCCEEEEEEHHHHH
SGLQLKAALTARGVHWPAIMITGHGDVNACRRAFKAGIVDFLTKPVDEQVLLDALAAAAK
CCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VLEEVLERQETHALLANLTEREREVMDLVCRGWASKEIAAALAVSPRTIDAHRANIAEKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
GTSSVADLVRMSIAAPPSR
CCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKPLRVYIVDDDAAVRQALSLLLSTCGMTVETFADPETFLAHVDTARPGCLLIDLRMPLV
CCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHCCCCCCEEEEEEHHHHH
SGLQLKAALTARGVHWPAIMITGHGDVNACRRAFKAGIVDFLTKPVDEQVLLDALAAAAK
CCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VLEEVLERQETHALLANLTEREREVMDLVCRGWASKEIAAALAVSPRTIDAHRANIAEKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
GTSSVADLVRMSIAAPPSR
CCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 2663474; 1857213; 10647182 [H]