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The map label for this gene is fixL [H]

Identifier: 154245498

GI number: 154245498

Start: 1763327

End: 1764916

Strand: Direct

Name: fixL [H]

Synonym: Xaut_1551

Alternate gene names: 154245498

Gene position: 1763327-1764916 (Clockwise)

Preceding gene: 154245496

Following gene: 154245499

Centisome position: 33.21

GC content: 71.51

Gene sequence:

>1590_bases
GTGCGCCACCGCATCTTTGCCTTCAGGTCGATCGCGGTTCTGGTCCTGGTCTGGATCGGGGCGGTGGGTGGCTTTGCGCT
GCTGTCCGCCCGTTCAGCCACGCAGCAGGCCGGCGAGGAGCTGAATGCCGCCGGCGTGGCACTGCATCGCATCGTCTCCC
AGCGTGTGGCGCAGCACGATGCCCACCTCACCAGCCTTGCGGCGCTGGTCCAGTCCGCCGATCCGCCGCCGCGCGAGGCG
GTGCGGCAGGTGGCGCTCGCCATCATGCGCTTTTATCCGCGCATCGTTTCGGTGCGCATCGTGGCGCCGGGCGCGGGCGG
GGGCGTCGAAATCCTCGTGGCAGCCCCGGACGGGCCGATGGCGACGCCCCTGCCGGCCTTCACCGCCGGCGTATTCGCCC
AGGCCCCGGGCGAGGCCCGCGCCTATGCCGAACCTGCGGTGCCCGGGCGTTATTTCCTCGGCAAGCGGACAGGAGGGCCG
GGTCGCCCGTTGGCCATGATCGTGGAGATCGACCCCGCTCTCCTGGTGGAAGGGGAGGAGCGCCCGGCCGCGACCCGCCT
GCGGCTGGATCTCGGCGGACACAGCCTGGTGGACCGCACCGCTACCGCCGGTCCGCAGGGGAGCGCGACGCTCGAGACGC
TGCACTTCGAACGCGCCATCGACAGCCAGACCCAGCCCCTGAAGCTGGCGCTGGACCGCAGCCTTGCGTTCGCGCAGATG
GTGCCCATGAGGCCGGTGCTGCTGTTCGCCGTCCTGTCCCTGATCGGCCTTCTCGCCCTGCGCTACACCCTGAAGCAGCG
CCGGGAGGCCAGTCGCTCGCGTGCGGCGGCCCGGGAGGCGCAGGAGCGCAGCCTTTTGCTGGAGCGCGAGACGCGCCTTG
CCCATGCCTCGCGGGTCAATGCGCTGGGCGAGCTCGCCTCCGGTATCGCCCATGAGCTGACCCAGCCGCTGACCGCGCTG
CTCAGCCAGAGCCAGGCGGCGCTGCGGCTGGCGGTGCCGGGGGGCGATGCCGTGCGGCTCGACGAGGCGCTGCGGGCCAA
TGTGCGCGAGGCCAAGCGCGCCGGCGAGATGCTCCAGCGCATGCGCGACTATATCAGCAACCGCGCTACCTCCCGCATGC
CCTCCGACATCAATGCCGTGATTGTCGATGCGGCCGCGCTGGCGCGCACCGATCTCGCCCAGCGGGGCATCGGCCTGGAG
CTGGATCTGGACCGCAAGCTGCCGCAGGCGGTGGTGGACCCGATCGAGCTGGAACAGGTGCTGCACAATCTCATCCGCAA
CGCGGTGGACAGCCTCGACGGCGCGGCGCAGCCCGAGAAGAAGATCGTCATCCGCACCGGGACCGGTGGCGGGCAAGTGG
AAATCCGCGTCGCCGACACCGGCCCGGGCATTGCGCCGGCAGTGCTGCCGCGCCTGTTCGAGCCCTTCTTCACCACCAAG
GCGGACGGCATGGGGCTGGGCCTGTCCCTGTGCGCGACGCTGGTGGAGCGGGCCGGCGGCTCCATCCGTGCGGATGCCGG
GGCGACCACGGGTGCGGCCTTCGTCATCATCCTGCCCGCCGCAGCGGCGCGCATGGAGGCGGCGCAATGA

Upstream 100 bases:

>100_bases
CCTGGCGGGAGGATCTTCCGTCGGCAGCGAAAATGGCCCCATATGGAGGCATTGCCGTCGTCATCCGGGGCGGCGAACGC
TTTCAGCAGGGGGAGATGGG

Downstream 100 bases:

>100_bases
AGCCCCTGCGCGTCTATATCGTGGACGATGACGCGGCGGTGCGGCAGGCGCTGTCGCTGCTGCTGTCCACCTGCGGCATG
ACGGTGGAGACCTTCGCCGA

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MRHRIFAFRSIAVLVLVWIGAVGGFALLSARSATQQAGEELNAAGVALHRIVSQRVAQHDAHLTSLAALVQSADPPPREA
VRQVALAIMRFYPRIVSVRIVAPGAGGGVEILVAAPDGPMATPLPAFTAGVFAQAPGEARAYAEPAVPGRYFLGKRTGGP
GRPLAMIVEIDPALLVEGEERPAATRLRLDLGGHSLVDRTATAGPQGSATLETLHFERAIDSQTQPLKLALDRSLAFAQM
VPMRPVLLFAVLSLIGLLALRYTLKQRREASRSRAAAREAQERSLLLERETRLAHASRVNALGELASGIAHELTQPLTAL
LSQSQAALRLAVPGGDAVRLDEALRANVREAKRAGEMLQRMRDYISNRATSRMPSDINAVIVDAAALARTDLAQRGIGLE
LDLDRKLPQAVVDPIELEQVLHNLIRNAVDSLDGAAQPEKKIVIRTGTGGGQVEIRVADTGPGIAPAVLPRLFEPFFTTK
ADGMGLGLSLCATLVERAGGSIRADAGATTGAAFVIILPAAAARMEAAQ

Sequences:

>Translated_529_residues
MRHRIFAFRSIAVLVLVWIGAVGGFALLSARSATQQAGEELNAAGVALHRIVSQRVAQHDAHLTSLAALVQSADPPPREA
VRQVALAIMRFYPRIVSVRIVAPGAGGGVEILVAAPDGPMATPLPAFTAGVFAQAPGEARAYAEPAVPGRYFLGKRTGGP
GRPLAMIVEIDPALLVEGEERPAATRLRLDLGGHSLVDRTATAGPQGSATLETLHFERAIDSQTQPLKLALDRSLAFAQM
VPMRPVLLFAVLSLIGLLALRYTLKQRREASRSRAAAREAQERSLLLERETRLAHASRVNALGELASGIAHELTQPLTAL
LSQSQAALRLAVPGGDAVRLDEALRANVREAKRAGEMLQRMRDYISNRATSRMPSDINAVIVDAAALARTDLAQRGIGLE
LDLDRKLPQAVVDPIELEQVLHNLIRNAVDSLDGAAQPEKKIVIRTGTGGGQVEIRVADTGPGIAPAVLPRLFEPFFTTK
ADGMGLGLSLCATLVERAGGSIRADAGATTGAAFVIILPAAAARMEAAQ
>Mature_529_residues
MRHRIFAFRSIAVLVLVWIGAVGGFALLSARSATQQAGEELNAAGVALHRIVSQRVAQHDAHLTSLAALVQSADPPPREA
VRQVALAIMRFYPRIVSVRIVAPGAGGGVEILVAAPDGPMATPLPAFTAGVFAQAPGEARAYAEPAVPGRYFLGKRTGGP
GRPLAMIVEIDPALLVEGEERPAATRLRLDLGGHSLVDRTATAGPQGSATLETLHFERAIDSQTQPLKLALDRSLAFAQM
VPMRPVLLFAVLSLIGLLALRYTLKQRREASRSRAAAREAQERSLLLERETRLAHASRVNALGELASGIAHELTQPLTAL
LSQSQAALRLAVPGGDAVRLDEALRANVREAKRAGEMLQRMRDYISNRATSRMPSDINAVIVDAAALARTDLAQRGIGLE
LDLDRKLPQAVVDPIELEQVLHNLIRNAVDSLDGAAQPEKKIVIRTGTGGGQVEIRVADTGPGIAPAVLPRLFEPFFTTK
ADGMGLGLSLCATLVERAGGSIRADAGATTGAAFVIILPAAAARMEAAQ

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=281, Percent_Identity=29.5373665480427, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1788549, Length=233, Percent_Identity=32.1888412017167, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI87081816, Length=268, Percent_Identity=28.7313432835821, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786783, Length=232, Percent_Identity=28.448275862069, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI145693157, Length=140, Percent_Identity=32.1428571428571, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790300, Length=224, Percent_Identity=30.8035714285714, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786912, Length=242, Percent_Identity=28.5123966942149, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789149, Length=265, Percent_Identity=22.6415094339623, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55975; Mature: 55975

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHRIFAFRSIAVLVLVWIGAVGGFALLSARSATQQAGEELNAAGVALHRIVSQRVAQHD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHLTSLAALVQSADPPPREAVRQVALAIMRFYPRIVSVRIVAPGAGGGVEILVAAPDGPM
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCCCCC
ATPLPAFTAGVFAQAPGEARAYAEPAVPGRYFLGKRTGGPGRPLAMIVEIDPALLVEGEE
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEEECCEEEEECCC
RPAATRLRLDLGGHSLVDRTATAGPQGSATLETLHFERAIDSQTQPLKLALDRSLAFAQM
CCCCEEEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VPMRPVLLFAVLSLIGLLALRYTLKQRREASRSRAAAREAQERSLLLERETRLAHASRVN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGELASGIAHELTQPLTALLSQSQAALRLAVPGGDAVRLDEALRANVREAKRAGEMLQR
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MRDYISNRATSRMPSDINAVIVDAAALARTDLAQRGIGLELDLDRKLPQAVVDPIELEQV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHCHHHHHHH
LHNLIRNAVDSLDGAAQPEKKIVIRTGTGGGQVEIRVADTGPGIAPAVLPRLFEPFFTTK
HHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCC
ADGMGLGLSLCATLVERAGGSIRADAGATTGAAFVIILPAAAARMEAAQ
CCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEECHHHHHHHCCC
>Mature Secondary Structure
MRHRIFAFRSIAVLVLVWIGAVGGFALLSARSATQQAGEELNAAGVALHRIVSQRVAQHD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHLTSLAALVQSADPPPREAVRQVALAIMRFYPRIVSVRIVAPGAGGGVEILVAAPDGPM
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCCCCC
ATPLPAFTAGVFAQAPGEARAYAEPAVPGRYFLGKRTGGPGRPLAMIVEIDPALLVEGEE
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEEECCEEEEECCC
RPAATRLRLDLGGHSLVDRTATAGPQGSATLETLHFERAIDSQTQPLKLALDRSLAFAQM
CCCCEEEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VPMRPVLLFAVLSLIGLLALRYTLKQRREASRSRAAAREAQERSLLLERETRLAHASRVN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGELASGIAHELTQPLTALLSQSQAALRLAVPGGDAVRLDEALRANVREAKRAGEMLQR
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MRDYISNRATSRMPSDINAVIVDAAALARTDLAQRGIGLELDLDRKLPQAVVDPIELEQV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHCHHHHHHH
LHNLIRNAVDSLDGAAQPEKKIVIRTGTGGGQVEIRVADTGPGIAPAVLPRLFEPFFTTK
HHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCC
ADGMGLGLSLCATLVERAGGSIRADAGATTGAAFVIILPAAAARMEAAQ
CCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEECHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]