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The map label for this gene is psdht [H]

Identifier: 154245453

GI number: 154245453

Start: 1713773

End: 1714795

Strand: Reverse

Name: psdht [H]

Synonym: Xaut_1506

Alternate gene names: 154245453

Gene position: 1714795-1713773 (Counterclockwise)

Preceding gene: 154245454

Following gene: 154245452

Centisome position: 32.3

GC content: 69.79

Gene sequence:

>1023_bases
ATGCCATCTCAATCCGCACTGTTCTCCGTCATTCCCGACACTTCTCATGTATTCGCCGCCACGATCGCTGACGTTGAGGC
AGCATATGATCGTATTTCCGGGCAGGTCATCCGCACCCCGCTCTTCGAGAATGCGGCCATCAACGCGGCCTGCGGGGCAC
GAATTCTCATCAAGCCGGAAATGTTGCAGCATACCGGCTCGTTCAAGTTCCGCGGTGCTTCCAATCGCGTGGCCCTGATG
AGCGCCGCCGAGCGGGCCGGCGGCATCGTCGCCTGGTCCTCGGGCAACCATGCCCTCGCCATCTCGGCGGTGGCCGCGCG
CCACGGCATCAAAGCCACCATCCTGATGCCCTCCGACGCCCCGCGCGCCAAGGTCGAGGGCGCCGAACGGCTCGGCGGCA
CCGTGCGCCTCTATGACCGCGCCACCGAAGTGCGCGAGGAGATCGGCGCCGACATCGCTGCCCGCACCGGCGCCGTCATC
ATCCCGCCTTATGACGACCCCCACGTCATCGCCGGCCAGGGCACGGTGGGCCTGGAACTGGCCGAGCAGGCGGCAGCCCT
CGGCGCGCAGCTCGACATGGCCCTGTTCGCCTGTTCCGGCGGCGGCCTGGTGGCGGGCGCCGCCACTGCCCTCAAGGCCC
GCTCGCCGGCAACCCTGGTCTATAGCGCCGAGCCCGCCGGCTTCGACGACACCGCCCGCTCGCTGGCCTCCGGCAAGCCG
GAGCGCAACGCGCCGGGCGCCACGACGCTGTGCGACGCCCTGATGGTGCCCACTCCCGGCCTGCTCACCTTCCCCATCAA
CCGCGCGCTGCTGGCGGGCGGCGTGGCGGTGACCGACGACGAAGTGAAGGCCGCCATGCGCCTCGTCTATGACACGCTGA
AGCTGGTGGTGGAGCCCGGCGGCGTCACCCCGCTCGCCGCCGTGCTGGCGGGAAAGGTGGACGCCAAGGGCAAGACCGTG
GGCGTCGTGCTCTCCGGTGGCAACGTGGACCCCGACACCTACGCCGCCTGCATCGGCGCCTGA

Upstream 100 bases:

>100_bases
TGGCCAATAAGCGGGCATGATTAAAGTTCATACAGGATCATAATTTGTATGCATGTATGGAGCGTGATGACATGGCGACC
TCTCCCGCCGAGGCCCGACC

Downstream 100 bases:

>100_bases
ACCCTTTCTTCTCGCTTCCAACCCACAGGGTTCACCATGCTCGCCAAGGACACGGCCCTTTATGCGCGGGTGGCGCGCAC
CATCGACGACTTCATGTGCC

Product: pyridoxal-5'-phosphate-dependent protein subunit beta

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MPSQSALFSVIPDTSHVFAATIADVEAAYDRISGQVIRTPLFENAAINAACGARILIKPEMLQHTGSFKFRGASNRVALM
SAAERAGGIVAWSSGNHALAISAVAARHGIKATILMPSDAPRAKVEGAERLGGTVRLYDRATEVREEIGADIAARTGAVI
IPPYDDPHVIAGQGTVGLELAEQAAALGAQLDMALFACSGGGLVAGAATALKARSPATLVYSAEPAGFDDTARSLASGKP
ERNAPGATTLCDALMVPTPGLLTFPINRALLAGGVAVTDDEVKAAMRLVYDTLKLVVEPGGVTPLAAVLAGKVDAKGKTV
GVVLSGGNVDPDTYAACIGA

Sequences:

>Translated_340_residues
MPSQSALFSVIPDTSHVFAATIADVEAAYDRISGQVIRTPLFENAAINAACGARILIKPEMLQHTGSFKFRGASNRVALM
SAAERAGGIVAWSSGNHALAISAVAARHGIKATILMPSDAPRAKVEGAERLGGTVRLYDRATEVREEIGADIAARTGAVI
IPPYDDPHVIAGQGTVGLELAEQAAALGAQLDMALFACSGGGLVAGAATALKARSPATLVYSAEPAGFDDTARSLASGKP
ERNAPGATTLCDALMVPTPGLLTFPINRALLAGGVAVTDDEVKAAMRLVYDTLKLVVEPGGVTPLAAVLAGKVDAKGKTV
GVVLSGGNVDPDTYAACIGA
>Mature_339_residues
PSQSALFSVIPDTSHVFAATIADVEAAYDRISGQVIRTPLFENAAINAACGARILIKPEMLQHTGSFKFRGASNRVALMS
AAERAGGIVAWSSGNHALAISAVAARHGIKATILMPSDAPRAKVEGAERLGGTVRLYDRATEVREEIGADIAARTGAVII
PPYDDPHVIAGQGTVGLELAEQAAALGAQLDMALFACSGGGLVAGAATALKARSPATLVYSAEPAGFDDTARSLASGKPE
RNAPGATTLCDALMVPTPGLLTFPINRALLAGGVAVTDDEVKAAMRLVYDTLKLVVEPGGVTPLAAVLAGKVDAKGKTVG
VVLSGGNVDPDTYAACIGA

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=319, Percent_Identity=36.0501567398119, Blast_Score=182, Evalue=4e-46,
Organism=Escherichia coli, GI1789505, Length=313, Percent_Identity=36.1022364217252, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1790207, Length=294, Percent_Identity=31.2925170068027, Blast_Score=114, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17508781, Length=317, Percent_Identity=31.8611987381703, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI71991565, Length=310, Percent_Identity=34.1935483870968, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17537387, Length=317, Percent_Identity=33.1230283911672, Blast_Score=127, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6322631, Length=319, Percent_Identity=40.4388714733542, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6320930, Length=320, Percent_Identity=31.5625, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21355833, Length=321, Percent_Identity=33.9563862928349, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24645328, Length=224, Percent_Identity=33.0357142857143, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 34563; Mature: 34432

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSQSALFSVIPDTSHVFAATIADVEAAYDRISGQVIRTPLFENAAINAACGARILIKPE
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEEECHH
MLQHTGSFKFRGASNRVALMSAAERAGGIVAWSSGNHALAISAVAARHGIKATILMPSDA
HHHCCCCEEEECCCCCEEEEEHHHHCCCEEEECCCCCEEEEEEEHHHCCCEEEEEECCCC
PRAKVEGAERLGGTVRLYDRATEVREEIGADIAARTGAVIIPPYDDPHVIAGQGTVGLEL
CCHHCCCHHHCCCEEEEEHHHHHHHHHHCCCHHHCCCEEEECCCCCCEEEECCCCCCHHH
AEQAAALGAQLDMALFACSGGGLVAGAATALKARSPATLVYSAEPAGFDDTARSLASGKP
HHHHHHHCCCEEEEEEEECCCCEEECHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCC
ERNAPGATTLCDALMVPTPGLLTFPINRALLAGGVAVTDDEVKAAMRLVYDTLKLVVEPG
CCCCCCHHHHHHHHHCCCCCEEEECCCHHHHHCCEEEEHHHHHHHHHHHHHHHHHEECCC
GVTPLAAVLAGKVDAKGKTVGVVLSGGNVDPDTYAACIGA
CCCHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHCCCC
>Mature Secondary Structure 
PSQSALFSVIPDTSHVFAATIADVEAAYDRISGQVIRTPLFENAAINAACGARILIKPE
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEEECHH
MLQHTGSFKFRGASNRVALMSAAERAGGIVAWSSGNHALAISAVAARHGIKATILMPSDA
HHHCCCCEEEECCCCCEEEEEHHHHCCCEEEECCCCCEEEEEEEHHHCCCEEEEEECCCC
PRAKVEGAERLGGTVRLYDRATEVREEIGADIAARTGAVIIPPYDDPHVIAGQGTVGLEL
CCHHCCCHHHCCCEEEEEHHHHHHHHHHCCCHHHCCCEEEECCCCCCEEEECCCCCCHHH
AEQAAALGAQLDMALFACSGGGLVAGAATALKARSPATLVYSAEPAGFDDTARSLASGKP
HHHHHHHCCCEEEEEEEECCCCEEECHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCC
ERNAPGATTLCDALMVPTPGLLTFPINRALLAGGVAVTDDEVKAAMRLVYDTLKLVVEPG
CCCCCCHHHHHHHHHCCCCCEEEECCCHHHHHCCEEEEHHHHHHHHHHHHHHHHHEECCC
GVTPLAAVLAGKVDAKGKTVGVVLSGGNVDPDTYAACIGA
CCCHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]