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The map label for this gene is kipI [H]

Identifier: 154245443

GI number: 154245443

Start: 1704812

End: 1705570

Strand: Reverse

Name: kipI [H]

Synonym: Xaut_1496

Alternate gene names: 154245443

Gene position: 1705570-1704812 (Counterclockwise)

Preceding gene: 154245444

Following gene: 154245442

Centisome position: 32.13

GC content: 71.01

Gene sequence:

>759_bases
TTGTCCCCTTCGCCTGAAAGTCCTGCGCCAGCCCATTCTGGCCCGCGCCTGCTGGATGCCGGGGAAACCGCCCTCGTCGC
CGAGTTCGGCGATGTGGTGGACCCCGTCCTCAACGACCGGGTGCTGGCCCTCGATGCGGCCGTCACCGCGCGCGCCCTGC
CCGGCGTGCGCGAGAGCGTGCCCACCTATCGCTCGCTCATGATTCACTACGATCCGCTGGTGATCGACCGCGCCACGCTC
GCCGCCGAGGTGACAGCGCTGGCGCAGGCAGCGCCTGCCGAGGCCCGGCACGGCGCGCTCTGGACCATCCCCTGCTGCTA
TGATCCCGAATTCGGCGAGGACATCGCCACCATCGCCGCCTCCACCGGCCTCACGCCGGACGAGGTGGTGAGGCTGCATT
CCGGCGCGCGCTACCGGCTCTACATGTACGGCTTCGCCCCCGGCTACTGCTATCTGGGCGGCCTGCCGGAGGCGTTCGCC
ACGGTCTCGCGCCGCTTGAAGCCGCGCCCGCCCCATCCGCCCAACGTGGTGATGATGGCGGGCGGGCTCGCCAGCATCGC
GACCGTCTCCATGCCCACCGGCTGGTTCCTCGTCGGCCGCACGCCGGAGCGCATGTTCTCCCTCACCCGCACCCCGACGG
TGCTGCTGGACGTGGGCGACGAACTCATGTTCGAGCCCATCGACCGCGCCACCTTCGACGCGCTGGAAGCCCGCGCGACG
GCAGGCGAAGTGGTTGCGCGCCGGGAGCCGCAGCCATGA

Upstream 100 bases:

>100_bases
CCCACCGAGATCGGCTCCATCTGCGTGCATGGCGATTCAGCCCATGCGGTGGAGACCGCGAGGCGGGTGCGCCGACGGCT
CGAAGACGAGGGCATTTCCA

Downstream 100 bases:

>100_bases
GCACTCCCGCCCTGCGCATCGTCGCCGCCGGTCCCGGCACCACCATCCAGGACAGCGGCCGGCACGGCTACCTCCGCTTC
GGCGTCACCGGGGCCGGCCC

Product: allophanate hydrolase subunit 1

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSPSPESPAPAHSGPRLLDAGETALVAEFGDVVDPVLNDRVLALDAAVTARALPGVRESVPTYRSLMIHYDPLVIDRATL
AAEVTALAQAAPAEARHGALWTIPCCYDPEFGEDIATIAASTGLTPDEVVRLHSGARYRLYMYGFAPGYCYLGGLPEAFA
TVSRRLKPRPPHPPNVVMMAGGLASIATVSMPTGWFLVGRTPERMFSLTRTPTVLLDVGDELMFEPIDRATFDALEARAT
AGEVVARREPQP

Sequences:

>Translated_252_residues
MSPSPESPAPAHSGPRLLDAGETALVAEFGDVVDPVLNDRVLALDAAVTARALPGVRESVPTYRSLMIHYDPLVIDRATL
AAEVTALAQAAPAEARHGALWTIPCCYDPEFGEDIATIAASTGLTPDEVVRLHSGARYRLYMYGFAPGYCYLGGLPEAFA
TVSRRLKPRPPHPPNVVMMAGGLASIATVSMPTGWFLVGRTPERMFSLTRTPTVLLDVGDELMFEPIDRATFDALEARAT
AGEVVARREPQP
>Mature_251_residues
SPSPESPAPAHSGPRLLDAGETALVAEFGDVVDPVLNDRVLALDAAVTARALPGVRESVPTYRSLMIHYDPLVIDRATLA
AEVTALAQAAPAEARHGALWTIPCCYDPEFGEDIATIAASTGLTPDEVVRLHSGARYRLYMYGFAPGYCYLGGLPEAFAT
VSRRLKPRPPHPPNVVMMAGGLASIATVSMPTGWFLVGRTPERMFSLTRTPTVLLDVGDELMFEPIDRATFDALEARATA
GEVVARREPQP

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=209, Percent_Identity=31.5789473684211, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 27010; Mature: 26879

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPSPESPAPAHSGPRLLDAGETALVAEFGDVVDPVLNDRVLALDAAVTARALPGVRESV
CCCCCCCCCCCCCCCCEECCCCEEEHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCHHHHC
PTYRSLMIHYDPLVIDRATLAAEVTALAQAAPAEARHGALWTIPCCYDPEFGEDIATIAA
CHHHHHEEECCCEEEEHHHHHHHHHHHHHHCCCCHHCCCEEEEEECCCCCCCHHHHHHHH
STGLTPDEVVRLHSGARYRLYMYGFAPGYCYLGGLPEAFATVSRRLKPRPPHPPNVVMMA
HCCCCHHHHHHHCCCCEEEEEEEECCCCEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEE
GGLASIATVSMPTGWFLVGRTPERMFSLTRTPTVLLDVGDELMFEPIDRATFDALEARAT
CCHHHHEEEECCCCEEEECCCHHHHHHHCCCCEEEEECCCHHHHCCCCHHHHHHHHHHHH
AGEVVARREPQP
HHHHHHCCCCCC
>Mature Secondary Structure 
SPSPESPAPAHSGPRLLDAGETALVAEFGDVVDPVLNDRVLALDAAVTARALPGVRESV
CCCCCCCCCCCCCCCEECCCCEEEHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCHHHHC
PTYRSLMIHYDPLVIDRATLAAEVTALAQAAPAEARHGALWTIPCCYDPEFGEDIATIAA
CHHHHHEEECCCEEEEHHHHHHHHHHHHHHCCCCHHCCCEEEEEECCCCCCCHHHHHHHH
STGLTPDEVVRLHSGARYRLYMYGFAPGYCYLGGLPEAFATVSRRLKPRPPHPPNVVMMA
HCCCCHHHHHHHCCCCEEEEEEEECCCCEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEE
GGLASIATVSMPTGWFLVGRTPERMFSLTRTPTVLLDVGDELMFEPIDRATFDALEARAT
CCHHHHEEEECCCCEEEECCCHHHHHHHCCCCEEEEECCCHHHHCCCCHHHHHHHHHHHH
AGEVVARREPQP
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]