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The map label for this gene is waaA [H]

Identifier: 154245429

GI number: 154245429

Start: 1691791

End: 1693086

Strand: Reverse

Name: waaA [H]

Synonym: Xaut_1482

Alternate gene names: 154245429

Gene position: 1693086-1691791 (Counterclockwise)

Preceding gene: 154245430

Following gene: 154245428

Centisome position: 31.89

GC content: 71.99

Gene sequence:

>1296_bases
ATGAAGGGTCGCGCCCTGCCCCTGACCCTGCGCCTCTACCGGGGCGCGAGCGCCGCGGCCTCGCTTCTCGCGCCGGCCTG
GCTCTCCTACCGGGTCCGCAAGGGCAAGGAAGACCCGACCCGCCTCGCCGAGCGGCGCGGGCACCCCAGCGCCGAGCGCC
CGGACGGGCCGCTGGTGTGGGTGCACGGGGCCAGCGTCGGCGAGGTGATCTCGGTGCTGCCGCTGATCGAGCGGCTCGAC
GCGCGCGGCTTCAAGGTGCTGTTGACCTCGGGCACCCTCACCTCCAGCCGGGTGGCCGCGGCCCGCACCCCGCCCGGGGT
GATCCACCAGTTCGTGCCCCTGGACGCGCGCGGCTTCATCGCCCGCTTCCTCGACTATTGGGCGCCCGACCTCGTGCTTC
TGGCCGAATCGGAGCTGTGGCCCAACCTGATCGCGGAGCTCGGCCGGCGCGGCACGCCGGTGGTGCTGGTGAATGCCCGG
CTGTCCGACCGCTCGGCCAAGCGCTGGGCACGCCTGCCCAAGAGCGCCCGCGCCTTGCTCTCGCGCATCGACCTGTGCCT
CGCCCAGAGCGAGGAGGACGCGGAGCGCTACCGCTCGCTGGGAACACCGCGGGTCGAAATCTGCGGCAATCTCAAGTTCG
ACGTGCCGCCGCCTGGCGTCGATCCCGCCGAGCTGAAGCGCTTCGGCGTCGCCGTGGGCAAGCGCCCGGTGCTGCTTGCC
GCCTCCACCCACGAGGGGGAGGAAGGCGCGATCATCGAGGCCCATCGCATCATCACCTCCCGAATTCCCGACCTGCTCAC
CATCATCGCTCCGCGCCATCCCGAGCGCGGTACCGAGGTGGCGGAGCTGGCCGAGGCCGCAGGCCTTGCCCCGCGCCAGC
GCGCCTTCGACGAGTGGCCCGACGCGGATACCGGCATCTATGTGGCCGACACCATCGGCGAGCTGGGCCTGTTCTACCGC
CTCGCGCAGGTGGCCTTCCTGGGCGGCTCGCTGGTGGAGCACGGCGGGCAGAATCCCATCGAGCCGGCCAAGCTTGGCAC
GGTGGTGCTCCACGGCCCCCACGTGTGGAACTTCGCCGCCGTCTATGACGCCCTCGACGCCGGCGAGGGCGCGGCGGAAG
TGGCCGACGCCATGGGCATCGCCCGCGCCGCCTATGCCCTGATCAAGGACAGCCAGCTCCAGGGCCACATGGCCGACGCA
GCCTTCGCCACGGTCACGGGGCTGGGTGGCGCGTTGGATCGCACCCTCTCGGCCATCGAGCCCTATCTCATCCAGATCCG
CCTCGAGGCCCGCTGA

Upstream 100 bases:

>100_bases
TGAAGGCGGCCCGCGACCTGGTGCAGGCCCGGCTCGAAGCCGCCACCGCCCGCGCCGAGGCCCTGGTCCTGGAAACCTCG
GCCGCCGGAGCCGACTCCGT

Downstream 100 bases:

>100_bases
GGATGCGCGCGCCCGCCTTCTGGTGGCGCCGCCCGGGTGTTGCGAGTGCCCTGCTCTCCCCCATCGGCGCCGTGGTGGGC
GCCGTCGCGCTGGCCCGCAT

Product: 3-deoxy-D-manno-octulosonic-acid transferase domain-containing protein

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKGRALPLTLRLYRGASAAASLLAPAWLSYRVRKGKEDPTRLAERRGHPSAERPDGPLVWVHGASVGEVISVLPLIERLD
ARGFKVLLTSGTLTSSRVAAARTPPGVIHQFVPLDARGFIARFLDYWAPDLVLLAESELWPNLIAELGRRGTPVVLVNAR
LSDRSAKRWARLPKSARALLSRIDLCLAQSEEDAERYRSLGTPRVEICGNLKFDVPPPGVDPAELKRFGVAVGKRPVLLA
ASTHEGEEGAIIEAHRIITSRIPDLLTIIAPRHPERGTEVAELAEAAGLAPRQRAFDEWPDADTGIYVADTIGELGLFYR
LAQVAFLGGSLVEHGGQNPIEPAKLGTVVLHGPHVWNFAAVYDALDAGEGAAEVADAMGIARAAYALIKDSQLQGHMADA
AFATVTGLGGALDRTLSAIEPYLIQIRLEAR

Sequences:

>Translated_431_residues
MKGRALPLTLRLYRGASAAASLLAPAWLSYRVRKGKEDPTRLAERRGHPSAERPDGPLVWVHGASVGEVISVLPLIERLD
ARGFKVLLTSGTLTSSRVAAARTPPGVIHQFVPLDARGFIARFLDYWAPDLVLLAESELWPNLIAELGRRGTPVVLVNAR
LSDRSAKRWARLPKSARALLSRIDLCLAQSEEDAERYRSLGTPRVEICGNLKFDVPPPGVDPAELKRFGVAVGKRPVLLA
ASTHEGEEGAIIEAHRIITSRIPDLLTIIAPRHPERGTEVAELAEAAGLAPRQRAFDEWPDADTGIYVADTIGELGLFYR
LAQVAFLGGSLVEHGGQNPIEPAKLGTVVLHGPHVWNFAAVYDALDAGEGAAEVADAMGIARAAYALIKDSQLQGHMADA
AFATVTGLGGALDRTLSAIEPYLIQIRLEAR
>Mature_431_residues
MKGRALPLTLRLYRGASAAASLLAPAWLSYRVRKGKEDPTRLAERRGHPSAERPDGPLVWVHGASVGEVISVLPLIERLD
ARGFKVLLTSGTLTSSRVAAARTPPGVIHQFVPLDARGFIARFLDYWAPDLVLLAESELWPNLIAELGRRGTPVVLVNAR
LSDRSAKRWARLPKSARALLSRIDLCLAQSEEDAERYRSLGTPRVEICGNLKFDVPPPGVDPAELKRFGVAVGKRPVLLA
ASTHEGEEGAIIEAHRIITSRIPDLLTIIAPRHPERGTEVAELAEAAGLAPRQRAFDEWPDADTGIYVADTIGELGLFYR
LAQVAFLGGSLVEHGGQNPIEPAKLGTVVLHGPHVWNFAAVYDALDAGEGAAEVADAMGIARAAYALIKDSQLQGHMADA
AFATVTGLGGALDRTLSAIEPYLIQIRLEAR

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=411, Percent_Identity=40.632603406326, Blast_Score=259, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 46439; Mature: 46439

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGRALPLTLRLYRGASAAASLLAPAWLSYRVRKGKEDPTRLAERRGHPSAERPDGPLVW
CCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCEEE
VHGASVGEVISVLPLIERLDARGFKVLLTSGTLTSSRVAAARTPPGVIHQFVPLDARGFI
EECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCHHHHH
ARFLDYWAPDLVLLAESELWPNLIAELGRRGTPVVLVNARLSDRSAKRWARLPKSARALL
HHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHH
SRIDLCLAQSEEDAERYRSLGTPRVEICGNLKFDVPPPGVDPAELKRFGVAVGKRPVLLA
HHHHHHHHCCHHHHHHHHHCCCCEEEEECCCEECCCCCCCCHHHHHHHHHHCCCCCEEEE
ASTHEGEEGAIIEAHRIITSRIPDLLTIIAPRHPERGTEVAELAEAAGLAPRQRAFDEWP
ECCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCC
DADTGIYVADTIGELGLFYRLAQVAFLGGSLVEHGGQNPIEPAKLGTVVLHGPHVWNFAA
CCCCCEEEEHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHCEEEEECCCHHHHHH
VYDALDAGEGAAEVADAMGIARAAYALIKDSQLQGHMADAAFATVTGLGGALDRTLSAIE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCC
PYLIQIRLEAR
CEEEEEEEECC
>Mature Secondary Structure
MKGRALPLTLRLYRGASAAASLLAPAWLSYRVRKGKEDPTRLAERRGHPSAERPDGPLVW
CCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCEEE
VHGASVGEVISVLPLIERLDARGFKVLLTSGTLTSSRVAAARTPPGVIHQFVPLDARGFI
EECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCHHHHH
ARFLDYWAPDLVLLAESELWPNLIAELGRRGTPVVLVNARLSDRSAKRWARLPKSARALL
HHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHH
SRIDLCLAQSEEDAERYRSLGTPRVEICGNLKFDVPPPGVDPAELKRFGVAVGKRPVLLA
HHHHHHHHCCHHHHHHHHHCCCCEEEEECCCEECCCCCCCCHHHHHHHHHHCCCCCEEEE
ASTHEGEEGAIIEAHRIITSRIPDLLTIIAPRHPERGTEVAELAEAAGLAPRQRAFDEWP
ECCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCC
DADTGIYVADTIGELGLFYRLAQVAFLGGSLVEHGGQNPIEPAKLGTVVLHGPHVWNFAA
CCCCCEEEEHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHCEEEEECCCHHHHHH
VYDALDAGEGAAEVADAMGIARAAYALIKDSQLQGHMADAAFATVTGLGGALDRTLSAIE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCC
PYLIQIRLEAR
CEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]