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The map label for this gene is ygfZ [C]

Identifier: 154245363

GI number: 154245363

Start: 1619987

End: 1620865

Strand: Direct

Name: ygfZ [C]

Synonym: Xaut_1416

Alternate gene names: 154245363

Gene position: 1619987-1620865 (Clockwise)

Preceding gene: 154245361

Following gene: 154245364

Centisome position: 30.51

GC content: 66.44

Gene sequence:

>879_bases
ATGCCCGTCGTTTATTTGACCGATCGTGTGCTCATTCGTGCCACCGGCCCCGAAGCCTCCAAGTTTCTCCATGGCGTGAT
CACCTGCAACGTGCAGACCATGGCGACGGGCGATGCGCGCTACGGCGCGCTTTTGACCCCGCAGGGCAAGATCATCTCCG
ACTTCCTGTTCTACGCGGAAGGCGACGACGCCTTCCTGTTCGACGTTCCCGCCGAGCGCGCCGAGGACCTGCTGAAGCGC
CTCACCTTCCATCGCCTGCGTGCGAAGGTCACCTTCACCAAGGCGGATGATCTCGCCGTCGCCGCTGTGTTCGGCGATGC
GGCGGAGGTGCCCGAGGGCGCGCTTTATCCCGACCCGCGCCTTGCCGCCCTGGGCCAGCGTCTGGTGCTGCCGCTGACGG
CGGCTCAGGCCCTGTCGTCGGATCCTGCGCTCTATGAGGCGCACCGCATCGCCCTCGGCATTCCCAAGGGCGGGCCCGAT
TTCACCTATGGCGACACCTTCCCCCACGAGGCCGATATGGACCAGCTGGGCGGGGTGGACTTCAAGAAGGGCTGCTATGT
GGGCCAGGAGGTGGTGAGCCGTATGGAGCATCGCTCCACCCCCCGCAACCGGCTGGTGGAAGTGCTGTTCGACACCCCCC
TCGCCACCGGGCAGGAGATCACCGCCGGCGACAAGAGCGTAGGGCAGGTGCTGTCGGTGACGGACGGGCGCGGCATCGCC
ACGGTGCGCCTCGATCGCGCCAACGACGCCAAGACCGACGGCGTGCCGCTGCTCGCCGGGGAGGTTCCGGTGGAGCTGCG
CCGTCCCGATTGGGCCGTCTTTTCCATGGAGTGGGAAAAGAAGGTGAGCGAGCTGGACCGCAAGTTCAAGGGAAGCTGA

Upstream 100 bases:

>100_bases
GCAGATCGAAAGTCACGGGCATGGGAGGAAGCTCGCTCTTGATGGGGACAGGCAAGCGGCCTTAAATCTGGTTTTTATTT
TTCTGACAAGGACGGCCCCC

Downstream 100 bases:

>100_bases
GAGGGGCGATGGCGGAGCCGAAGGCGGGAAACGCGAACAAGAGTGCGCCGGAGGCGGAAAGCGCGAACGGGATCGATCTG
GCGGTGAAGGCGGAACGCGC

Product: glycine cleavage T protein (aminomethyl transferase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MPVVYLTDRVLIRATGPEASKFLHGVITCNVQTMATGDARYGALLTPQGKIISDFLFYAEGDDAFLFDVPAERAEDLLKR
LTFHRLRAKVTFTKADDLAVAAVFGDAAEVPEGALYPDPRLAALGQRLVLPLTAAQALSSDPALYEAHRIALGIPKGGPD
FTYGDTFPHEADMDQLGGVDFKKGCYVGQEVVSRMEHRSTPRNRLVEVLFDTPLATGQEITAGDKSVGQVLSVTDGRGIA
TVRLDRANDAKTDGVPLLAGEVPVELRRPDWAVFSMEWEKKVSELDRKFKGS

Sequences:

>Translated_292_residues
MPVVYLTDRVLIRATGPEASKFLHGVITCNVQTMATGDARYGALLTPQGKIISDFLFYAEGDDAFLFDVPAERAEDLLKR
LTFHRLRAKVTFTKADDLAVAAVFGDAAEVPEGALYPDPRLAALGQRLVLPLTAAQALSSDPALYEAHRIALGIPKGGPD
FTYGDTFPHEADMDQLGGVDFKKGCYVGQEVVSRMEHRSTPRNRLVEVLFDTPLATGQEITAGDKSVGQVLSVTDGRGIA
TVRLDRANDAKTDGVPLLAGEVPVELRRPDWAVFSMEWEKKVSELDRKFKGS
>Mature_291_residues
PVVYLTDRVLIRATGPEASKFLHGVITCNVQTMATGDARYGALLTPQGKIISDFLFYAEGDDAFLFDVPAERAEDLLKRL
TFHRLRAKVTFTKADDLAVAAVFGDAAEVPEGALYPDPRLAALGQRLVLPLTAAQALSSDPALYEAHRIALGIPKGGPDF
TYGDTFPHEADMDQLGGVDFKKGCYVGQEVVSRMEHRSTPRNRLVEVLFDTPLATGQEITAGDKSVGQVLSVTDGRGIAT
VRLDRANDAKTDGVPLLAGEVPVELRRPDWAVFSMEWEKKVSELDRKFKGS

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Homo sapiens, GI58197556, Length=268, Percent_Identity=29.8507462686567, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1789265, Length=237, Percent_Identity=26.1603375527426, Blast_Score=64, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI71985951, Length=272, Percent_Identity=27.9411764705882, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24645123, Length=296, Percent_Identity=30.0675675675676, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 31792; Mature: 31660

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVVYLTDRVLIRATGPEASKFLHGVITCNVQTMATGDARYGALLTPQGKIISDFLFYAE
CCEEEEECEEEEEECCCCHHHHEEEEEEEEEEEEECCCCCCCEEECCCCHHEEEEEEEEC
GDDAFLFDVPAERAEDLLKRLTFHRLRAKVTFTKADDLAVAAVFGDAAEVPEGALYPDPR
CCCEEEEECCHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEEEECCHHHCCCCCCCCCCH
LAALGQRLVLPLTAAQALSSDPALYEAHRIALGIPKGGPDFTYGDTFPHEADMDQLGGVD
HHHCCCCEEEEEHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCC
FKKGCYVGQEVVSRMEHRSTPRNRLVEVLFDTPLATGQEITAGDKSVGQVLSVTDGRGIA
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEECCCCHHHHHEEEECCCCCEE
TVRLDRANDAKTDGVPLLAGEVPVELRRPDWAVFSMEWEKKVSELDRKFKGS
EEEEECCCCCCCCCEEEEECCCCEEEECCCEEEEEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVVYLTDRVLIRATGPEASKFLHGVITCNVQTMATGDARYGALLTPQGKIISDFLFYAE
CEEEEECEEEEEECCCCHHHHEEEEEEEEEEEEECCCCCCCEEECCCCHHEEEEEEEEC
GDDAFLFDVPAERAEDLLKRLTFHRLRAKVTFTKADDLAVAAVFGDAAEVPEGALYPDPR
CCCEEEEECCHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEEEECCHHHCCCCCCCCCCH
LAALGQRLVLPLTAAQALSSDPALYEAHRIALGIPKGGPDFTYGDTFPHEADMDQLGGVD
HHHCCCCEEEEEHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCC
FKKGCYVGQEVVSRMEHRSTPRNRLVEVLFDTPLATGQEITAGDKSVGQVLSVTDGRGIA
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEECCCCHHHHHEEEECCCCCEE
TVRLDRANDAKTDGVPLLAGEVPVELRRPDWAVFSMEWEKKVSELDRKFKGS
EEEEECCCCCCCCCEEEEECCCCEEEECCCEEEEEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA