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The map label for this gene is purD [H]

Identifier: 154245359

GI number: 154245359

Start: 1615255

End: 1616523

Strand: Reverse

Name: purD [H]

Synonym: Xaut_1412

Alternate gene names: 154245359

Gene position: 1616523-1615255 (Counterclockwise)

Preceding gene: 154245362

Following gene: 154245358

Centisome position: 30.45

GC content: 69.74

Gene sequence:

>1269_bases
ATGAACATCCTGCTGCTCGGATCGGGAGGGCGCGAGCACGCCCTCGCCTGGAAGCTGGCGCAGAGCCCAAGTATGGGCCA
GTTCTACGCCATACCGGGCAATCCCGGCATTGCCGAGCACGCGGAGCTGATCGAGGGCATCCCCCTCTCGGCCCACGACA
CCATCGTGAAATGGTGCCAGGACCACGGCATAGACTTCGTGGTGGTGGGTCCCGAGGCGCCGCTGGTGGCGGGGCTGGTG
GACCGGCTGGAAGCCGTCGGCATTCCCGCCTTCGGCCCCACGGCCGCCGCCGCCCGGCTGGAGGGCTCCAAGCTTTTCAC
CAAGGAATTGTGCAAGGCCAACTCCATTCCCACCGCCGCCTTCGGCCGCTTCACCTCGGCCGCCGAGGGCGCCGCCTATG
TGCGCCAGCAGGGCGCGCCCATCGTGGTGAAGGCGGACGGCCTCATGGCCGGCAAAGGCGTCGTGGTCGCCCTTTCCGAG
GAGGAGGCGGTGGACGCGGTGGAGCAGGTGTTCGCCGGCGGCGGCACCGAGGTGCTCATCGAGGAATTCATGGAGGGCGA
AGAGGCCAGCCTGTTCGCTTTGGTGGATGGCGAGACGGTGGTGCTCTTCGGCAGCGCCCAGGACCACAAGCGGGCCTTCG
ACGGCGACCTCGGCCCCAACACCGGCGGCATGGGCGCCTATTCGCCCGCCCCCGTCCTGACGCCCGAGCTGGAGCGCCGG
GCTCTGGACGAAATCCTCATCCCCACCGCCCGCGCCATGGTGGCGGCCGGCGCGCCCTATCGCGGCGTGCTCTATGCCGG
ACTCATGCTGACGGCCGACGGGCCGAAGCTGGTGGAATACAATTGCCGCTTCGGCGATCCCGAATGCCAGGTGCTGATGC
CCCGCTTTAAGGGTGACCTGGTGCAGGTGCTGCACGCCACCGCCACTGGCCAACTCGCGCAGGTGACGCCGGAGTGGTCG
CAGGATGCGGCCATGACGGTGGTCTATGCCTCGCGCGGCTATCCCGGCCCGCATCAGGTGGGCAGCCCCATCGGCGGGAT
CGAGGCGGCGGCGGAACTCGACGGCGTGCTGGTGTTCCAGGCCGGCACACGGCGCAAGGGCGTCGCGCTGGTTTCGAACG
GCGGGCGCGTGCTGAACGTCACCGCCACCGGCGCCACCCTCGGCGAGGCCCGTGACCGGGCCTATGCTGGGATCGCCCGC
ATCGATTGGCCGGACGGCTTCTACCGCAGCGATATCGGCTGGCGCGCGCTCGGCAAGGGGGCCCGATGA

Upstream 100 bases:

>100_bases
CGGCCCTGAGCCGATAGGCGATTGTGACAAGAGACTCTCGCAAGAGACGCCAGTCAGAGGCGTACGAGCGAGACATGGGG
CAACGCACGGGGGAGGCCGC

Downstream 100 bases:

>100_bases
CCGCCCGCGAGAAGCCGCCCCTGCCCACCCTGGACGATTTTCCGGTCAAGGCGAGCGACACCATCCGCTACGGCGACACC
GACAAGCTCGGCCATGTGAA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MNILLLGSGGREHALAWKLAQSPSMGQFYAIPGNPGIAEHAELIEGIPLSAHDTIVKWCQDHGIDFVVVGPEAPLVAGLV
DRLEAVGIPAFGPTAAAARLEGSKLFTKELCKANSIPTAAFGRFTSAAEGAAYVRQQGAPIVVKADGLMAGKGVVVALSE
EEAVDAVEQVFAGGGTEVLIEEFMEGEEASLFALVDGETVVLFGSAQDHKRAFDGDLGPNTGGMGAYSPAPVLTPELERR
ALDEILIPTARAMVAAGAPYRGVLYAGLMLTADGPKLVEYNCRFGDPECQVLMPRFKGDLVQVLHATATGQLAQVTPEWS
QDAAMTVVYASRGYPGPHQVGSPIGGIEAAAELDGVLVFQAGTRRKGVALVSNGGRVLNVTATGATLGEARDRAYAGIAR
IDWPDGFYRSDIGWRALGKGAR

Sequences:

>Translated_422_residues
MNILLLGSGGREHALAWKLAQSPSMGQFYAIPGNPGIAEHAELIEGIPLSAHDTIVKWCQDHGIDFVVVGPEAPLVAGLV
DRLEAVGIPAFGPTAAAARLEGSKLFTKELCKANSIPTAAFGRFTSAAEGAAYVRQQGAPIVVKADGLMAGKGVVVALSE
EEAVDAVEQVFAGGGTEVLIEEFMEGEEASLFALVDGETVVLFGSAQDHKRAFDGDLGPNTGGMGAYSPAPVLTPELERR
ALDEILIPTARAMVAAGAPYRGVLYAGLMLTADGPKLVEYNCRFGDPECQVLMPRFKGDLVQVLHATATGQLAQVTPEWS
QDAAMTVVYASRGYPGPHQVGSPIGGIEAAAELDGVLVFQAGTRRKGVALVSNGGRVLNVTATGATLGEARDRAYAGIAR
IDWPDGFYRSDIGWRALGKGAR
>Mature_422_residues
MNILLLGSGGREHALAWKLAQSPSMGQFYAIPGNPGIAEHAELIEGIPLSAHDTIVKWCQDHGIDFVVVGPEAPLVAGLV
DRLEAVGIPAFGPTAAAARLEGSKLFTKELCKANSIPTAAFGRFTSAAEGAAYVRQQGAPIVVKADGLMAGKGVVVALSE
EEAVDAVEQVFAGGGTEVLIEEFMEGEEASLFALVDGETVVLFGSAQDHKRAFDGDLGPNTGGMGAYSPAPVLTPELERR
ALDEILIPTARAMVAAGAPYRGVLYAGLMLTADGPKLVEYNCRFGDPECQVLMPRFKGDLVQVLHATATGQLAQVTPEWS
QDAAMTVVYASRGYPGPHQVGSPIGGIEAAAELDGVLVFQAGTRRKGVALVSNGGRVLNVTATGATLGEARDRAYAGIAR
IDWPDGFYRSDIGWRALGKGAR

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=47.5177304964539, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=47.5177304964539, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=47.5177304964539, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI28416899, Length=423, Percent_Identity=47.5177304964539, Blast_Score=377, Evalue=1e-104,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=49.5327102803738, Blast_Score=400, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17567511, Length=424, Percent_Identity=40.3301886792453, Blast_Score=280, Evalue=9e-76,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=40.4545454545455, Blast_Score=301, Evalue=1e-82,
Organism=Drosophila melanogaster, GI62471619, Length=429, Percent_Identity=41.4918414918415, Blast_Score=288, Evalue=5e-78,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=41.8604651162791, Blast_Score=288, Evalue=7e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44093; Mature: 44093

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILLLGSGGREHALAWKLAQSPSMGQFYAIPGNPGIAEHAELIEGIPLSAHDTIVKWCQ
CEEEEECCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHH
DHGIDFVVVGPEAPLVAGLVDRLEAVGIPAFGPTAAAARLEGSKLFTKELCKANSIPTAA
HCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCCCHH
FGRFTSAAEGAAYVRQQGAPIVVKADGLMAGKGVVVALSEEEAVDAVEQVFAGGGTEVLI
HHCCHHHCCCHHHHHHCCCCEEEEECCEEECCCEEEEECCHHHHHHHHHHHCCCCHHHHH
EEFMEGEEASLFALVDGETVVLFGSAQDHKRAFDGDLGPNTGGMGAYSPAPVLTPELERR
HHHHCCCCCEEEEEECCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
ALDEILIPTARAMVAAGAPYRGVLYAGLMLTADGPKLVEYNCRFGDPECQVLMPRFKGDL
HHHHHHCCHHHHHHHCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCEEECCHHCCHH
VQVLHATATGQLAQVTPEWSQDAAMTVVYASRGYPGPHQVGSPIGGIEAAAELDGVLVFQ
HHHHHHHCCCCEEECCCCCCCCCEEEEEEECCCCCCHHHHCCCCCCHHHHHHCCCEEEEE
AGTRRKGVALVSNGGRVLNVTATGATLGEARDRAYAGIARIDWPDGFYRSDIGWRALGKG
CCCCCCCEEEEECCCEEEEEEECCCCHHHHHHHHHCCEEEEECCCCCEECCCCHHHHCCC
AR
CC
>Mature Secondary Structure
MNILLLGSGGREHALAWKLAQSPSMGQFYAIPGNPGIAEHAELIEGIPLSAHDTIVKWCQ
CEEEEECCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHH
DHGIDFVVVGPEAPLVAGLVDRLEAVGIPAFGPTAAAARLEGSKLFTKELCKANSIPTAA
HCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCCCHH
FGRFTSAAEGAAYVRQQGAPIVVKADGLMAGKGVVVALSEEEAVDAVEQVFAGGGTEVLI
HHCCHHHCCCHHHHHHCCCCEEEEECCEEECCCEEEEECCHHHHHHHHHHHCCCCHHHHH
EEFMEGEEASLFALVDGETVVLFGSAQDHKRAFDGDLGPNTGGMGAYSPAPVLTPELERR
HHHHCCCCCEEEEEECCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
ALDEILIPTARAMVAAGAPYRGVLYAGLMLTADGPKLVEYNCRFGDPECQVLMPRFKGDL
HHHHHHCCHHHHHHHCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCEEECCHHCCHH
VQVLHATATGQLAQVTPEWSQDAAMTVVYASRGYPGPHQVGSPIGGIEAAAELDGVLVFQ
HHHHHHHCCCCEEECCCCCCCCCEEEEEEECCCCCCHHHHCCCCCCHHHHHHCCCEEEEE
AGTRRKGVALVSNGGRVLNVTATGATLGEARDRAYAGIARIDWPDGFYRSDIGWRALGKG
CCCCCCCEEEEECCCEEEEEEECCCCHHHHHHHHHCCEEEEECCCCCEECCCCHHHHCCC
AR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]