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The map label for this gene is ispE

Identifier: 154245328

GI number: 154245328

Start: 1583993

End: 1584874

Strand: Reverse

Name: ispE

Synonym: Xaut_1381

Alternate gene names: 154245328

Gene position: 1584874-1583993 (Counterclockwise)

Preceding gene: 154245332

Following gene: 154245327

Centisome position: 29.85

GC content: 73.81

Gene sequence:

>882_bases
ATGGAACTGATGCGCGCGCGCGCCCCGGCGAAGATCAACCTGACGTTGCGCATCGAAGGCCGTCGCGCCGACGGCTTTCA
CGATCTCGCGAGCCTCGTCGCCTTTGCCGGCGCCGGCGACCTCGTTTTCCTCCATCCCGATGCGCCCTTGGCGCTGGAGG
TCTGCGGCCCCACGGCCGCCGCCGCCGGCCCGGACGACGACAATCTGGTGCTGCGCGCGGCCCGTGCCTTTGCGGAACGG
GTGCCCGGCGCGCGGCTCGGCCGTTTCGTGCTGGAAAAGCGGCTGCCGGTGGCGGCGGGACTGGGGGGCGGCTCGTCCGA
TGCCGCCGCCGCGCTGCGCCTGCTCGCCGGCCTCAACGGGCTGGCGCTGGATGATCCGCGCCTGTTTGCGGCCGCCCGCG
CCACCGGATCGGACGTGCCGGTGTGCCTTGATCCACGGGCGCGGCTGATGCGCGGGGTGGGCGATGTGCTCTCGGCCCCG
CTCGCCCTGCCGCCCTTGTCGGCGGTGCTGGTGAATTGCCGGGTCGCCGTGCCCACTGCCGGCGTGTTCCGTGCGCTGGG
CCTTGCGCCGGGCGCGGCTTTGGCTGGTCCCGAGCACCCGGCCGGTCTGCCGGAGACGACGATGGGGAATGCGGCGGCCG
TGTGCGCCTTCCTGTCCGGCCTGCCGAACGATCTGGAGCCGCCGGCCCTGGCGCTGGCGCCGGAGATCGGCCGGGCCATG
GCCCTGCTTTCCGAAGCGCCCGAGGCGCGGCTGGTGCGCATGTCCGGCTCCGGCGCAACGGTGTTCGCCCTCACCGACAG
CTGCCGCACCGCCGCATCGCTGGCACGGCGCCTGCTTGCGCAGCAGCCCGGCTGGTGGGTGAAGCCCACACTTCTGCGAT
AG

Upstream 100 bases:

>100_bases
GGGCGGTGGTGCGGTGCGGGAGAGGGGCGAGGGGGAGAGGGCAAGGGGAGAAGGCGGCCCGGGCGTGACGCCACGGCCCG
ATCTGCTATAAGCCCGGCCC

Downstream 100 bases:

>100_bases
GGCTGAACCCCACCATGACGCGCCCCCCGAAGACGTTGCCCCGATGACGCTGCCCGCCTTCCTCTTCGCCGCCCTTGGGG
AAATCGCCGGCTGTTTCGCG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MELMRARAPAKINLTLRIEGRRADGFHDLASLVAFAGAGDLVFLHPDAPLALEVCGPTAAAAGPDDDNLVLRAARAFAER
VPGARLGRFVLEKRLPVAAGLGGGSSDAAAALRLLAGLNGLALDDPRLFAAARATGSDVPVCLDPRARLMRGVGDVLSAP
LALPPLSAVLVNCRVAVPTAGVFRALGLAPGAALAGPEHPAGLPETTMGNAAAVCAFLSGLPNDLEPPALALAPEIGRAM
ALLSEAPEARLVRMSGSGATVFALTDSCRTAASLARRLLAQQPGWWVKPTLLR

Sequences:

>Translated_293_residues
MELMRARAPAKINLTLRIEGRRADGFHDLASLVAFAGAGDLVFLHPDAPLALEVCGPTAAAAGPDDDNLVLRAARAFAER
VPGARLGRFVLEKRLPVAAGLGGGSSDAAAALRLLAGLNGLALDDPRLFAAARATGSDVPVCLDPRARLMRGVGDVLSAP
LALPPLSAVLVNCRVAVPTAGVFRALGLAPGAALAGPEHPAGLPETTMGNAAAVCAFLSGLPNDLEPPALALAPEIGRAM
ALLSEAPEARLVRMSGSGATVFALTDSCRTAASLARRLLAQQPGWWVKPTLLR
>Mature_293_residues
MELMRARAPAKINLTLRIEGRRADGFHDLASLVAFAGAGDLVFLHPDAPLALEVCGPTAAAAGPDDDNLVLRAARAFAER
VPGARLGRFVLEKRLPVAAGLGGGSSDAAAALRLLAGLNGLALDDPRLFAAARATGSDVPVCLDPRARLMRGVGDVLSAP
LALPPLSAVLVNCRVAVPTAGVFRALGLAPGAALAGPEHPAGLPETTMGNAAAVCAFLSGLPNDLEPPALALAPEIGRAM
ALLSEAPEARLVRMSGSGATVFALTDSCRTAASLARRLLAQQPGWWVKPTLLR

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=290, Percent_Identity=32.0689655172414, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_XANP2 (A7IF36)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001416286.1
- ProteinModelPortal:   A7IF36
- SMR:   A7IF36
- STRING:   A7IF36
- GeneID:   5420740
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_1381
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   GMGVARA
- ProtClustDB:   PRK14608
- BioCyc:   XAUT78245:XAUT_1381-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 29949; Mature: 29949

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 138-138

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELMRARAPAKINLTLRIEGRRADGFHDLASLVAFAGAGDLVFLHPDAPLALEVCGPTAA
CCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCC
AAGPDDDNLVLRAARAFAERVPGARLGRFVLEKRLPVAAGLGGGSSDAAAALRLLAGLNG
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCC
LALDDPRLFAAARATGSDVPVCLDPRARLMRGVGDVLSAPLALPPLSAVLVNCRVAVPTA
CCCCCCHHHEEHHCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHCEEECCCH
GVFRALGLAPGAALAGPEHPAGLPETTMGNAAAVCAFLSGLPNDLEPPALALAPEIGRAM
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEECCHHHHHHH
ALLSEAPEARLVRMSGSGATVFALTDSCRTAASLARRLLAQQPGWWVKPTLLR
HHHHCCCCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MELMRARAPAKINLTLRIEGRRADGFHDLASLVAFAGAGDLVFLHPDAPLALEVCGPTAA
CCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCC
AAGPDDDNLVLRAARAFAERVPGARLGRFVLEKRLPVAAGLGGGSSDAAAALRLLAGLNG
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCC
LALDDPRLFAAARATGSDVPVCLDPRARLMRGVGDVLSAPLALPPLSAVLVNCRVAVPTA
CCCCCCHHHEEHHCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHCEEECCCH
GVFRALGLAPGAALAGPEHPAGLPETTMGNAAAVCAFLSGLPNDLEPPALALAPEIGRAM
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEECCHHHHHHH
ALLSEAPEARLVRMSGSGATVFALTDSCRTAASLARRLLAQQPGWWVKPTLLR
HHHHCCCCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA