Click here to switch to the map view.

The map label for this gene is ispB [H]

Identifier: 154245320

GI number: 154245320

Start: 1575483

End: 1576490

Strand: Reverse

Name: ispB [H]

Synonym: Xaut_1373

Alternate gene names: 154245320

Gene position: 1576490-1575483 (Counterclockwise)

Preceding gene: 154245325

Following gene: 154245318

Centisome position: 29.7

GC content: 68.35

Gene sequence:

>1008_bases
TTGGCCCTCGTCCTACCGTTCGAGCCCAACGAGCCGTCCATCGAGGCCCTGGTCGCCCTGGTGAAAGCGGACATGGAACG
CGTGAACGCCACCATCCTGGCGCGCACCGGCTCCGACGTGGCGATGATTCCCGAGGTGGCGAACCACCTCATCTCCTCCG
GCGGCAAGCGCTTGCGGCCGATGCTGACGCTCGCCTGCGCGTCGCTCTCCGGCTATCGGGGCGACGGGCATGTGAAGCTC
GCGGCGTCCGTGGAGTTCATGCACACCGCCACCCTCCTCCACGACGACGTGGTGGACGAGAGCGACATGCGCCGGGGCAA
GCTCGCCGCCCGCAAGCTGTGGGGCAACGAGGCCAGCGTGCTGGTGGGCGACTTCCTGCTCGGCCAGGCGTTCAAGATGA
TGGTGGAGGTGGGCTCCATGACCTGCCTCGACATCCTCGCCACCGCCGCCTGCGTCATCGCCGAGGGTGAGGTGATGCAG
CTCGCCGCCGCCAAGAACACCGCCACCTCGGAAGACGATTATCTCGCCGTCATCCGCGGCAAGACCGCCGAATTGTTCGC
CGCCGCCTGCGAGGTGGGGGCGGTGCTGGGCCAAAGGCCCAAGGCCGAGCAGGCCGCCTGCCGCTCCTACGGCATGAATC
TCGGCATCGCCTTCCAGCTGGTGGACGACGCCCTCGACTATGGTGGCAGCCAGGCCAAGCTCGGCAAAAATGTGGGCGAC
GACTTCCGCGAGGGGAAGATCACCCTGCCGGTGCTGCTCGCCTTCCGCCGCGGCGACGCCAGTGAGCGCGCCTTCTGGCA
GCGCTGCCTGGAGAAGGGCGAGGGCAACGACGCCGATCTGGATCAGGCCATCGGGCTGATGACCCGCCACCGCGCCATCG
CCGACACGGTGGAGCGCGCCCGCCATTACGGCGCCATCGCCAAGGATGCGCTGGCCCTGTTCCCCAACGGGCAGGCCAAG
GACGCGCTGATGGAAGCGGTGGATTTCTGCCTGTCCCGCGCGCACTGA

Upstream 100 bases:

>100_bases
GTGCGCACCGGCGCGACGCGAAGAAAACCGCCCGGCGCTTGCACGGAGCCCGTCAGCATCCCTATGGTGCGCCCCAAAGA
ATGACAAGCGGGAGCGTTTC

Downstream 100 bases:

>100_bases
CGCCACCGGTCTCGGTCGTTGACCGAGACCGGACGGGCACACTCAGACGCCGCGCGGGCTTGACGAACGGCCAATGACCG
CGACACGCATCCCGAACAGC

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MALVLPFEPNEPSIEALVALVKADMERVNATILARTGSDVAMIPEVANHLISSGGKRLRPMLTLACASLSGYRGDGHVKL
AASVEFMHTATLLHDDVVDESDMRRGKLAARKLWGNEASVLVGDFLLGQAFKMMVEVGSMTCLDILATAACVIAEGEVMQ
LAAAKNTATSEDDYLAVIRGKTAELFAAACEVGAVLGQRPKAEQAACRSYGMNLGIAFQLVDDALDYGGSQAKLGKNVGD
DFREGKITLPVLLAFRRGDASERAFWQRCLEKGEGNDADLDQAIGLMTRHRAIADTVERARHYGAIAKDALALFPNGQAK
DALMEAVDFCLSRAH

Sequences:

>Translated_335_residues
MALVLPFEPNEPSIEALVALVKADMERVNATILARTGSDVAMIPEVANHLISSGGKRLRPMLTLACASLSGYRGDGHVKL
AASVEFMHTATLLHDDVVDESDMRRGKLAARKLWGNEASVLVGDFLLGQAFKMMVEVGSMTCLDILATAACVIAEGEVMQ
LAAAKNTATSEDDYLAVIRGKTAELFAAACEVGAVLGQRPKAEQAACRSYGMNLGIAFQLVDDALDYGGSQAKLGKNVGD
DFREGKITLPVLLAFRRGDASERAFWQRCLEKGEGNDADLDQAIGLMTRHRAIADTVERARHYGAIAKDALALFPNGQAK
DALMEAVDFCLSRAH
>Mature_334_residues
ALVLPFEPNEPSIEALVALVKADMERVNATILARTGSDVAMIPEVANHLISSGGKRLRPMLTLACASLSGYRGDGHVKLA
ASVEFMHTATLLHDDVVDESDMRRGKLAARKLWGNEASVLVGDFLLGQAFKMMVEVGSMTCLDILATAACVIAEGEVMQL
AAAKNTATSEDDYLAVIRGKTAELFAAACEVGAVLGQRPKAEQAACRSYGMNLGIAFQLVDDALDYGGSQAKLGKNVGDD
FREGKITLPVLLAFRRGDASERAFWQRCLEKGEGNDADLDQAIGLMTRHRAIADTVERARHYGAIAKDALALFPNGQAKD
ALMEAVDFCLSRAH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=296, Percent_Identity=30.0675675675676, Blast_Score=134, Evalue=9e-32,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=42.6791277258567, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI1786623, Length=223, Percent_Identity=32.2869955156951, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17505681, Length=240, Percent_Identity=35.8333333333333, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319475, Length=307, Percent_Identity=30.6188925081433, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6325188, Length=233, Percent_Identity=22.3175965665236, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24651612, Length=272, Percent_Identity=30.8823529411765, Blast_Score=131, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35869; Mature: 35737

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVLPFEPNEPSIEALVALVKADMERVNATILARTGSDVAMIPEVANHLISSGGKRLRP
CEEEEECCCCCCHHHHHHHHHHHHHHHHCEEEEEECCCCEEHHHHHHHHHHHCCCHHHHH
MLTLACASLSGYRGDGHVKLAASVEFMHTATLLHDDVVDESDMRRGKLAARKLWGNEASV
HHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHH
LVGDFLLGQAFKMMVEVGSMTCLDILATAACVIAEGEVMQLAAAKNTATSEDDYLAVIRG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCEEEEEEC
KTAELFAAACEVGAVLGQRPKAEQAACRSYGMNLGIAFQLVDDALDYGGSQAKLGKNVGD
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCC
DFREGKITLPVLLAFRRGDASERAFWQRCLEKGEGNDADLDQAIGLMTRHRAIADTVERA
HHHCCCEEHHHHHEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RHYGAIAKDALALFPNGQAKDALMEAVDFCLSRAH
HHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALVLPFEPNEPSIEALVALVKADMERVNATILARTGSDVAMIPEVANHLISSGGKRLRP
EEEEECCCCCCHHHHHHHHHHHHHHHHCEEEEEECCCCEEHHHHHHHHHHHCCCHHHHH
MLTLACASLSGYRGDGHVKLAASVEFMHTATLLHDDVVDESDMRRGKLAARKLWGNEASV
HHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHH
LVGDFLLGQAFKMMVEVGSMTCLDILATAACVIAEGEVMQLAAAKNTATSEDDYLAVIRG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCEEEEEEC
KTAELFAAACEVGAVLGQRPKAEQAACRSYGMNLGIAFQLVDDALDYGGSQAKLGKNVGD
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCC
DFREGKITLPVLLAFRRGDASERAFWQRCLEKGEGNDADLDQAIGLMTRHRAIADTVERA
HHHCCCEEHHHHHEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RHYGAIAKDALALFPNGQAKDALMEAVDFCLSRAH
HHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]