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The map label for this gene is leuA [H]

Identifier: 154245277

GI number: 154245277

Start: 1524798

End: 1526381

Strand: Direct

Name: leuA [H]

Synonym: Xaut_1329

Alternate gene names: 154245277

Gene position: 1524798-1526381 (Clockwise)

Preceding gene: 154245276

Following gene: 154245278

Centisome position: 28.72

GC content: 67.55

Gene sequence:

>1584_bases
ATGACCGACACTTCCTCTGCCCCCGTCTCCGGGAAGCCCGTATCCGACCGCGACCGCGTGGTCATCTTCGACACCACCCT
GCGCGACGGCGAGCAGTGCCCCGGCGCCTCCATGACCTTCGAGGAGAAGCTGGAAGTGGCCGCGCTCCTCGACGAGATGG
GCGTGGACGTGATCGAGGCCGGCTTCCCCATCGCCTCCATCGGCGATTTCGAGAGCGTGGCCGAGATCGCGCGGCGCACG
CAGAACGCCACCGTCGCCGGCCTGTCGCGCGCCGCGCTGAACGACATCGACCGCTGCGCCGAGGCGGTGAAGCACGCCCG
CCGCGGCCGCATCCACACCTTCCTGTCCACCTCTCCGGTGCACATGAAGTACAAGCTCCAGAAGCAGCCCCACGAGGTGC
TGGAGATGGTGGTGGCGTCCGTCACCCGCGCCCGCAACCATGTGGAGGACGTGGAGTGGTCGTCCGAGGACGGCACCCGC
ACCGAGATCGACTTCCTGTGCCGCTGCGTGGAAGCCGCCATCAAGGCCGGCGCCACCACCATCAACATCCCCGACACCGT
GGGCTACACCACCCCGGCCGAGTATGAGGCCCTGTTCCGCACCGTGATCGAGCGCGTGCCGAACTCGGACAAGGCCATTT
TCTCGGTGCATTGCCATGACGACCTCGGCATGGCGGTGGCGAACTCGCTGGCCGGCCTCGCCGGCGGCGCGCGGCAGATC
GAGTGCACCGTCAACGGCATCGGCGAGCGCGCGGGCAATGCGGCGCTCGAAGAAGTGGTGATGGCCATCGAGACCCGCTC
GGACGTGCTGCCCTATCGCTCCGGCATCAACACCAAGATGCTCACCCGCGCCTCCAAGCTGGTATCGGCGGTGACCTCGT
TCCCAGTGCAGTACAACAAGGCCATCGTGGGCCGGAACGCCTTCGCCCATGAGAGCGGCATCCACCAGGACGGCATGCTC
AAGCACACCCAGACCTATGAGATCATGACGCCGGAGAGCGTCGGCGTCTCCAAGACCTCGCTGGTCATGGGCAAGCATTC
GGGCCGCGCCGCCTTCCGCGACAAGCTCAAGACCATGGGCTACGAGCTGGGCGAGAACGCCCTGAACGACGCCTTCACCC
GCTTCAAGGACCTCGCCGACCGCAAGAAGGTGGTCTACGACGAGGACATCGAGGCGCTGGTGGACCAGGGCATCGCCGCC
GCCTACGACCGCATCAAGCTCATCTCCCTGTCGGTCATCGCCGGCACCCGCGGGCCGCAGCGCGCCACCATGCGGCTGGA
GGTGGACGGCCAGGTGAAGACCGAGGAAGCCGAGGGCAACGGCCCGGTGGACGCCACCTTCAACGCCATCAAGGCGCTGG
TGCCCCACCAGGTGAAGCTCGACCTCTACCAGGTCCATGCCGTGACCGAGGGCACCGACGCCCAGGCCGAGGTCTCGGTA
CGGCTGGAAGAGAACGGCAAGGCCGTGACCGCCCGCGCGGCGGACCCGGACACGCTGGTCGCCTCGGCGCAGGCCTACAT
CACCGCGCTCAACAAGCTGCACATGAAGCGCCAGACCATGAACCCGCAGAGCGCCGTGGGCTGA

Upstream 100 bases:

>100_bases
CCGTCGGGTTGAAGACCCGGCGCCCGTTTCGGCTTATGATCCGGCAAAACCGCGCCCGACCGATGACGGGCTCCGCCGGA
CCGCCAGAAGGACCGTTCCG

Downstream 100 bases:

>100_bases
CCCCTCGCGCCGGTCCCGATGCGAAAGGCCGCCCCGTTGGGCGGCCTTTTTCTTTGGGCGGATGTCTTTGGGCGGCGGTG
TGGCGCGGGCGGCTCCATCC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MTDTSSAPVSGKPVSDRDRVVIFDTTLRDGEQCPGASMTFEEKLEVAALLDEMGVDVIEAGFPIASIGDFESVAEIARRT
QNATVAGLSRAALNDIDRCAEAVKHARRGRIHTFLSTSPVHMKYKLQKQPHEVLEMVVASVTRARNHVEDVEWSSEDGTR
TEIDFLCRCVEAAIKAGATTINIPDTVGYTTPAEYEALFRTVIERVPNSDKAIFSVHCHDDLGMAVANSLAGLAGGARQI
ECTVNGIGERAGNAALEEVVMAIETRSDVLPYRSGINTKMLTRASKLVSAVTSFPVQYNKAIVGRNAFAHESGIHQDGML
KHTQTYEIMTPESVGVSKTSLVMGKHSGRAAFRDKLKTMGYELGENALNDAFTRFKDLADRKKVVYDEDIEALVDQGIAA
AYDRIKLISLSVIAGTRGPQRATMRLEVDGQVKTEEAEGNGPVDATFNAIKALVPHQVKLDLYQVHAVTEGTDAQAEVSV
RLEENGKAVTARAADPDTLVASAQAYITALNKLHMKRQTMNPQSAVG

Sequences:

>Translated_527_residues
MTDTSSAPVSGKPVSDRDRVVIFDTTLRDGEQCPGASMTFEEKLEVAALLDEMGVDVIEAGFPIASIGDFESVAEIARRT
QNATVAGLSRAALNDIDRCAEAVKHARRGRIHTFLSTSPVHMKYKLQKQPHEVLEMVVASVTRARNHVEDVEWSSEDGTR
TEIDFLCRCVEAAIKAGATTINIPDTVGYTTPAEYEALFRTVIERVPNSDKAIFSVHCHDDLGMAVANSLAGLAGGARQI
ECTVNGIGERAGNAALEEVVMAIETRSDVLPYRSGINTKMLTRASKLVSAVTSFPVQYNKAIVGRNAFAHESGIHQDGML
KHTQTYEIMTPESVGVSKTSLVMGKHSGRAAFRDKLKTMGYELGENALNDAFTRFKDLADRKKVVYDEDIEALVDQGIAA
AYDRIKLISLSVIAGTRGPQRATMRLEVDGQVKTEEAEGNGPVDATFNAIKALVPHQVKLDLYQVHAVTEGTDAQAEVSV
RLEENGKAVTARAADPDTLVASAQAYITALNKLHMKRQTMNPQSAVG
>Mature_526_residues
TDTSSAPVSGKPVSDRDRVVIFDTTLRDGEQCPGASMTFEEKLEVAALLDEMGVDVIEAGFPIASIGDFESVAEIARRTQ
NATVAGLSRAALNDIDRCAEAVKHARRGRIHTFLSTSPVHMKYKLQKQPHEVLEMVVASVTRARNHVEDVEWSSEDGTRT
EIDFLCRCVEAAIKAGATTINIPDTVGYTTPAEYEALFRTVIERVPNSDKAIFSVHCHDDLGMAVANSLAGLAGGARQIE
CTVNGIGERAGNAALEEVVMAIETRSDVLPYRSGINTKMLTRASKLVSAVTSFPVQYNKAIVGRNAFAHESGIHQDGMLK
HTQTYEIMTPESVGVSKTSLVMGKHSGRAAFRDKLKTMGYELGENALNDAFTRFKDLADRKKVVYDEDIEALVDQGIAAA
YDRIKLISLSVIAGTRGPQRATMRLEVDGQVKTEEAEGNGPVDATFNAIKALVPHQVKLDLYQVHAVTEGTDAQAEVSVR
LEENGKAVTARAADPDTLVASAQAYITALNKLHMKRQTMNPQSAVG

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=496, Percent_Identity=48.7903225806452, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6320071, Length=403, Percent_Identity=27.0471464019851, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320019, Length=398, Percent_Identity=27.3869346733668, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6324225, Length=496, Percent_Identity=25.4032258064516, Blast_Score=117, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6324682, Length=500, Percent_Identity=24.2, Blast_Score=110, Evalue=4e-25,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 57059; Mature: 56928

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTSSAPVSGKPVSDRDRVVIFDTTLRDGEQCPGASMTFEEKLEVAALLDEMGVDVIEA
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHC
GFPIASIGDFESVAEIARRTQNATVAGLSRAALNDIDRCAEAVKHARRGRIHTFLSTSPV
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCE
HMKYKLQKQPHEVLEMVVASVTRARNHVEDVEWSSEDGTRTEIDFLCRCVEAAIKAGATT
EEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
INIPDTVGYTTPAEYEALFRTVIERVPNSDKAIFSVHCHDDLGMAVANSLAGLAGGARQI
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEE
ECTVNGIGERAGNAALEEVVMAIETRSDVLPYRSGINTKMLTRASKLVSAVTSFPVQYNK
EEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHCCHHHCC
AIVGRNAFAHESGIHQDGMLKHTQTYEIMTPESVGVSKTSLVMGKHSGRAAFRDKLKTMG
EEECCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHEEEEECCCCHHHHHHHHHHHH
YELGENALNDAFTRFKDLADRKKVVYDEDIEALVDQGIAAAYDRIKLISLSVIAGTRGPQ
HHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHEEEEEEEEEECCCCCC
RATMRLEVDGQVKTEEAEGNGPVDATFNAIKALVPHQVKLDLYQVHAVTEGTDAQAEVSV
CEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCEEEEE
RLEENGKAVTARAADPDTLVASAQAYITALNKLHMKRQTMNPQSAVG
EEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure 
TDTSSAPVSGKPVSDRDRVVIFDTTLRDGEQCPGASMTFEEKLEVAALLDEMGVDVIEA
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHC
GFPIASIGDFESVAEIARRTQNATVAGLSRAALNDIDRCAEAVKHARRGRIHTFLSTSPV
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCE
HMKYKLQKQPHEVLEMVVASVTRARNHVEDVEWSSEDGTRTEIDFLCRCVEAAIKAGATT
EEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
INIPDTVGYTTPAEYEALFRTVIERVPNSDKAIFSVHCHDDLGMAVANSLAGLAGGARQI
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEE
ECTVNGIGERAGNAALEEVVMAIETRSDVLPYRSGINTKMLTRASKLVSAVTSFPVQYNK
EEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHCCHHHCC
AIVGRNAFAHESGIHQDGMLKHTQTYEIMTPESVGVSKTSLVMGKHSGRAAFRDKLKTMG
EEECCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHEEEEECCCCHHHHHHHHHHHH
YELGENALNDAFTRFKDLADRKKVVYDEDIEALVDQGIAAAYDRIKLISLSVIAGTRGPQ
HHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHEEEEEEEEEECCCCCC
RATMRLEVDGQVKTEEAEGNGPVDATFNAIKALVPHQVKLDLYQVHAVTEGTDAQAEVSV
CEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCEEEEE
RLEENGKAVTARAADPDTLVASAQAYITALNKLHMKRQTMNPQSAVG
EEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA