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The map label for this gene is phnJ [H]

Identifier: 154245261

GI number: 154245261

Start: 1508301

End: 1509200

Strand: Reverse

Name: phnJ [H]

Synonym: Xaut_1313

Alternate gene names: 154245261

Gene position: 1509200-1508301 (Counterclockwise)

Preceding gene: 154245262

Following gene: 154245260

Centisome position: 28.43

GC content: 65.22

Gene sequence:

>900_bases
ATGATGCTTGAATATATTTCCATGCCCGCCATCACCTACAATTTCGCCTATCTGGACGAGCAGACGAAACGGATGATCCG
CCGGGCCATCCTGAAGGCCATCGCCATCCCCGGCTATCAGGTGCCGTTCGCCTCGCGGGAGATGCCCATGCCCTATGGCT
GGGGCACCGGCGGCGTGCAGGTGACCGCCGCCATCCTCGGCGCGAAAGATGTGCTCAAGGTGATCGACCAGGGCTCGGAC
GACACCACCAACGCAGTGGCCATCCGCACGTTCTTCGAGACCACGGCGGGGGTGGCCACCACCACGCGCACGCAGGACGC
CACCATCATCCAGACCCGCCATCGCATTCCCGAGGCGCCGCTGAGGGAAGGCCAAGTCATCGTCTACCAGGTGCCCATCC
CCGAGCCGCTGCGCTACCTGGAGCCGCGCGAGACCGAGACGCGGCGCCTGCACGCCCTGTCCGACTATGGCCTGATGCAG
GTGAAGCTCTACGAGGACATCGCGAAGCATGGCCACATCGCCACCACCTACGCCTATCCGGTGAAGGTGGCGGGCCGCTA
TGTGATGGACCCCTCGCCCATCCCCAAGTTCGACAATCCGAAGATGGACGACAGCCCGGCGTTGCAGCTGTTCGGCGCGG
GGCGCGAGAAGCGCATCTATGCCATTCCGCCCTATACCTGCGTGGTGTCGCTGGATTTCGAGGACCACCCGTTCGCGGTG
CAGGCCTTCGCCGAGCCCTGCGCCCTGTGCGGCGCCAAGGGGGTCTATCTCGACGAGGTGATCCTCGACGACAAGGGCGG
CCGCATGTTCGTTTGCTCCGACACAGACCATTGCGCCACCCGGCAGGCCGAAGGCCACACCGGCTCCATGAGCGCGCCCT
ATGGGGAGGGCCAGCCATGA

Upstream 100 bases:

>100_bases
ACAATTAGGAGCATATCAACTCAAACAGGAAGAACACCACAAGAAATTGACCTTTGGCTGTATCATTATGATAAGAAAAA
TCAAGCATCAAAGGAGCGGA

Downstream 100 bases:

>100_bases
GCCAGGAGATGATCTCGCCCCCCGACGCGCCTCTCCTCGTCGCCGAGGGACTTGAGAAATATTACGGTCCCCGCCTCGGC
TGCCGCGACGTGGACCTCAC

Product: phosphonate metabolism PhnJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MMLEYISMPAITYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTAAILGAKDVLKVIDQGSD
DTTNAVAIRTFFETTAGVATTTRTQDATIIQTRHRIPEAPLREGQVIVYQVPIPEPLRYLEPRETETRRLHALSDYGLMQ
VKLYEDIAKHGHIATTYAYPVKVAGRYVMDPSPIPKFDNPKMDDSPALQLFGAGREKRIYAIPPYTCVVSLDFEDHPFAV
QAFAEPCALCGAKGVYLDEVILDDKGGRMFVCSDTDHCATRQAEGHTGSMSAPYGEGQP

Sequences:

>Translated_299_residues
MMLEYISMPAITYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTAAILGAKDVLKVIDQGSD
DTTNAVAIRTFFETTAGVATTTRTQDATIIQTRHRIPEAPLREGQVIVYQVPIPEPLRYLEPRETETRRLHALSDYGLMQ
VKLYEDIAKHGHIATTYAYPVKVAGRYVMDPSPIPKFDNPKMDDSPALQLFGAGREKRIYAIPPYTCVVSLDFEDHPFAV
QAFAEPCALCGAKGVYLDEVILDDKGGRMFVCSDTDHCATRQAEGHTGSMSAPYGEGQP
>Mature_299_residues
MMLEYISMPAITYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTAAILGAKDVLKVIDQGSD
DTTNAVAIRTFFETTAGVATTTRTQDATIIQTRHRIPEAPLREGQVIVYQVPIPEPLRYLEPRETETRRLHALSDYGLMQ
VKLYEDIAKHGHIATTYAYPVKVAGRYVMDPSPIPKFDNPKMDDSPALQLFGAGREKRIYAIPPYTCVVSLDFEDHPFAV
QAFAEPCALCGAKGVYLDEVILDDKGGRMFVCSDTDHCATRQAEGHTGSMSAPYGEGQP

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3627

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnJ family [H]

Homologues:

Organism=Escherichia coli, GI1790536, Length=266, Percent_Identity=78.5714285714286, Blast_Score=457, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010306 [H]

Pfam domain/function: PF06007 PhnJ [H]

EC number: NA

Molecular weight: Translated: 33116; Mature: 33116

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLEYISMPAITYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQ
CEEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCE
VTAAILGAKDVLKVIDQGSDDTTNAVAIRTFFETTAGVATTTRTQDATIIQTRHRIPEAP
EEHHHHCHHHHHHHHHCCCCCCCCEEEEEEEHHHHCCCEEECCCCCCEEEEHHHCCCCCC
LREGQVIVYQVPIPEPLRYLEPRETETRRLHALSDYGLMQVKLYEDIAKHGHIATTYAYP
CCCCCEEEEECCCCCHHHHCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHCCCEEEEEEEE
VKVAGRYVMDPSPIPKFDNPKMDDSPALQLFGAGREKRIYAIPPYTCVVSLDFEDHPFAV
EEECCEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEECCEEEEEEECCCCCCCHH
QAFAEPCALCGAKGVYLDEVILDDKGGRMFVCSDTDHCATRQAEGHTGSMSAPYGEGQP
HHHHHHHHHHCCCCCEEEEEEEECCCCEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMLEYISMPAITYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQ
CEEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCE
VTAAILGAKDVLKVIDQGSDDTTNAVAIRTFFETTAGVATTTRTQDATIIQTRHRIPEAP
EEHHHHCHHHHHHHHHCCCCCCCCEEEEEEEHHHHCCCEEECCCCCCEEEEHHHCCCCCC
LREGQVIVYQVPIPEPLRYLEPRETETRRLHALSDYGLMQVKLYEDIAKHGHIATTYAYP
CCCCCEEEEECCCCCHHHHCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHCCCEEEEEEEE
VKVAGRYVMDPSPIPKFDNPKMDDSPALQLFGAGREKRIYAIPPYTCVVSLDFEDHPFAV
EEECCEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEECCEEEEEEECCCCCCCHH
QAFAEPCALCGAKGVYLDEVILDDKGGRMFVCSDTDHCATRQAEGHTGSMSAPYGEGQP
HHHHHHHHHHCCCCCEEEEEEEECCCCEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]