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The map label for this gene is nfdA [H]

Identifier: 154245222

GI number: 154245222

Start: 1467365

End: 1469341

Strand: Reverse

Name: nfdA [H]

Synonym: Xaut_1274

Alternate gene names: 154245222

Gene position: 1469341-1467365 (Counterclockwise)

Preceding gene: 154245223

Following gene: 154245221

Centisome position: 27.68

GC content: 68.39

Gene sequence:

>1977_bases
ATGCCCGACCGAAAGCTGCCCGACCGAAAGCTGCCCGATCGCCGCCAGTTCATCGCCGCGGGGCTCATGGCCGCCGCCGG
CCTTGTCCCCACCTCCCCGAAGGCGCAGACCATGAGCGAGACCGCCGACCTCATCCTCTTCAACGGCAAGGTCACCACCC
TCGACCGCACCAATCCGCAGGCTGAGGCGGTGGCGGTGAAGGACGGGCGCATCCTGTTCGCCGGCACCACCGCCGACGCC
ATGCGCCACGCCGGGACCCTGACGAAAATCATCGACGTGGGCGGCCGGCGGATCATTCCCGGCCTCATCGACAGCCACAT
GCACATCATCCGCGGCGGGCTGAACTACAATATGGAGCTGCGCTGGGACGGGGTGCGCTCCCTCGACCACGCCATGGAGA
TGCTGAGGAAACAGGTGGCCGTGACCCCGCCGCCGCAATGGGTGCGCGTGGTGGGCGGCTTCACCGAGCACCAGTTCGCC
GAGAAGCGCCTGCCCACGCTGGAGGAGCTGAACGCCGCCGCCCCCGACACGCCGGTGTTCATCCTCCACCTCTACGACCG
CGCTTTGCTCAATGCGGCGGCCCTGCGCGTGGTAGGCTACACCAAGGACACGCCCAACCCGCCCGGCGGCGAGATCGTGC
GCGATGCGGCCGGCAATCCCACCGGCCTGCTTTTGGCCCAGCCCAACGCCACCATCCTCTATTCGACGCTGGCCAAGGGT
CCCAAGCTGCCCGCCGAATACCAGAAGAACTCCACCCGCCACTTCATGCGCGAAGTGAACCGGCTGGGCGTCACCAGCGT
CATCGACGCCGGCGGCGGCTTCCAGAACTATCCGGACGATTATGCGGTGATCGAGGAGTTGCACCGCGACGGGCAGCTTT
CCGTGCGCATCGCCTACAATCTCTTCACCCAGAAGCCGAAGCAGGAGCTGGCGGATTTCTCCGGCTGGTCGAAACAGGTC
AAGCCCGGCCAGGGCGACGACCTCTACCGCAACAACGGCGCCGGCGAGATGCTGGTCTATTCCGCCGCCGACTTCGAGGA
CTTCCAGGTGGCCCGGCCCGACATGCCGCCCAACATGGAAGGCGACCTGGAACCGGTCATCCGCCTGCTGGCCGAGAACC
GCTGGCCGTGGCGGCTGCACGCCACCTATGACGAGACCATCTCGCGCGCCCTCGACGTGTTCGAGAAGGTGAACAAGGAC
GTGCCGCTCAAGGGCCTCAACTGGTTCTTCGACCATGCCGAGACCATCACCGACCGCAACATCGACCGCATCGCGGCGCT
GGGGGGCGGCATCGCCGTGCAGCATCGCATGGCGTTCCAGGGCGAATATTTCGTGGAGCGCTACGGCGCCGGCAGGGCGG
AGCGCTCCCCGCCCATCCGCCGCATGCTGGACGCCGGCATTCCGGTGGGCGCCGGCACCGACGCCACCCGCGTCGCCTCC
TACAATCCCTGGGTGTCCCTGTCCTGGCTGGTGACGGGCAAGACTTTGGGCGGCCTCTCCCTCTACCCGCCGTCCAACCT
GCTCGACCGCGAGAGTGCGCTGCGGCTGTGGACTGAGGCCAATACCTGGTTCTCCTCCGAGCCCGACAAGAAGGGGCAGG
TGAAGGCCGGCCAGCTCGCCGACCTCGTGGTGCTGTCGGACGATTATTTCTCCGTGCCCGAGGACAGGATCCAGGACATC
ACCAGCGTGCTCACGCTGCTGGGCGGCACGCCGGTGCATGGCGACGGCGACTTCAAGACGCTCGCGCCCGACCTGCCGCC
GCCCATGCCCGACTGGTCGCCGGTGCGCACCTTCGGCGGCTACCAGAAGCGGGCGGAAACCGGCGAGCGCAAATACGCCT
TCGCCGCCGCCTGCGGCTGCGCCACCGCCTGCGGCGTCCACGGCCACGCCCACGCCAGCGCGCTGGCGGCGCAGGTGCCC
GCCGCCGACGAACGCTCCTTCTGGGGCGCGCTCGGCTGCGCCTGCTGGGCGTTCTGA

Upstream 100 bases:

>100_bases
CAACTGCCGGGCCGGCAGAGCGCCGCAGCGGAGCCGCCGCAAGACGCGCATCTCAACACGCCGCCTCACACCCCTCCCGG
AACCTGAGCGGAAGAGCGCC

Downstream 100 bases:

>100_bases
TGGGGGCGGAGGTGGTGGCGCCTTGAGATGCTTGCACACCGTCGCCGTCCGGCTTGACCGGACGGCCCATCGCCCCGCCG
GACCGACGCCTCCGGGACAA

Product: amidohydrolase 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 658; Mature: 657

Protein sequence:

>658_residues
MPDRKLPDRKLPDRRQFIAAGLMAAAGLVPTSPKAQTMSETADLILFNGKVTTLDRTNPQAEAVAVKDGRILFAGTTADA
MRHAGTLTKIIDVGGRRIIPGLIDSHMHIIRGGLNYNMELRWDGVRSLDHAMEMLRKQVAVTPPPQWVRVVGGFTEHQFA
EKRLPTLEELNAAAPDTPVFILHLYDRALLNAAALRVVGYTKDTPNPPGGEIVRDAAGNPTGLLLAQPNATILYSTLAKG
PKLPAEYQKNSTRHFMREVNRLGVTSVIDAGGGFQNYPDDYAVIEELHRDGQLSVRIAYNLFTQKPKQELADFSGWSKQV
KPGQGDDLYRNNGAGEMLVYSAADFEDFQVARPDMPPNMEGDLEPVIRLLAENRWPWRLHATYDETISRALDVFEKVNKD
VPLKGLNWFFDHAETITDRNIDRIAALGGGIAVQHRMAFQGEYFVERYGAGRAERSPPIRRMLDAGIPVGAGTDATRVAS
YNPWVSLSWLVTGKTLGGLSLYPPSNLLDRESALRLWTEANTWFSSEPDKKGQVKAGQLADLVVLSDDYFSVPEDRIQDI
TSVLTLLGGTPVHGDGDFKTLAPDLPPPMPDWSPVRTFGGYQKRAETGERKYAFAAACGCATACGVHGHAHASALAAQVP
AADERSFWGALGCACWAF

Sequences:

>Translated_658_residues
MPDRKLPDRKLPDRRQFIAAGLMAAAGLVPTSPKAQTMSETADLILFNGKVTTLDRTNPQAEAVAVKDGRILFAGTTADA
MRHAGTLTKIIDVGGRRIIPGLIDSHMHIIRGGLNYNMELRWDGVRSLDHAMEMLRKQVAVTPPPQWVRVVGGFTEHQFA
EKRLPTLEELNAAAPDTPVFILHLYDRALLNAAALRVVGYTKDTPNPPGGEIVRDAAGNPTGLLLAQPNATILYSTLAKG
PKLPAEYQKNSTRHFMREVNRLGVTSVIDAGGGFQNYPDDYAVIEELHRDGQLSVRIAYNLFTQKPKQELADFSGWSKQV
KPGQGDDLYRNNGAGEMLVYSAADFEDFQVARPDMPPNMEGDLEPVIRLLAENRWPWRLHATYDETISRALDVFEKVNKD
VPLKGLNWFFDHAETITDRNIDRIAALGGGIAVQHRMAFQGEYFVERYGAGRAERSPPIRRMLDAGIPVGAGTDATRVAS
YNPWVSLSWLVTGKTLGGLSLYPPSNLLDRESALRLWTEANTWFSSEPDKKGQVKAGQLADLVVLSDDYFSVPEDRIQDI
TSVLTLLGGTPVHGDGDFKTLAPDLPPPMPDWSPVRTFGGYQKRAETGERKYAFAAACGCATACGVHGHAHASALAAQVP
AADERSFWGALGCACWAF
>Mature_657_residues
PDRKLPDRKLPDRRQFIAAGLMAAAGLVPTSPKAQTMSETADLILFNGKVTTLDRTNPQAEAVAVKDGRILFAGTTADAM
RHAGTLTKIIDVGGRRIIPGLIDSHMHIIRGGLNYNMELRWDGVRSLDHAMEMLRKQVAVTPPPQWVRVVGGFTEHQFAE
KRLPTLEELNAAAPDTPVFILHLYDRALLNAAALRVVGYTKDTPNPPGGEIVRDAAGNPTGLLLAQPNATILYSTLAKGP
KLPAEYQKNSTRHFMREVNRLGVTSVIDAGGGFQNYPDDYAVIEELHRDGQLSVRIAYNLFTQKPKQELADFSGWSKQVK
PGQGDDLYRNNGAGEMLVYSAADFEDFQVARPDMPPNMEGDLEPVIRLLAENRWPWRLHATYDETISRALDVFEKVNKDV
PLKGLNWFFDHAETITDRNIDRIAALGGGIAVQHRMAFQGEYFVERYGAGRAERSPPIRRMLDAGIPVGAGTDATRVASY
NPWVSLSWLVTGKTLGGLSLYPPSNLLDRESALRLWTEANTWFSSEPDKKGQVKAGQLADLVVLSDDYFSVPEDRIQDIT
SVLTLLGGTPVHGDGDFKTLAPDLPPPMPDWSPVRTFGGYQKRAETGERKYAFAAACGCATACGVHGHAHASALAAQVPA
ADERSFWGALGCACWAF

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 72148; Mature: 72016

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDRKLPDRKLPDRRQFIAAGLMAAAGLVPTSPKAQTMSETADLILFNGKVTTLDRTNPQ
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCEEEECCEEEEEECCCCC
AEAVAVKDGRILFAGTTADAMRHAGTLTKIIDVGGRRIIPGLIDSHMHIIRGGLNYNMEL
CEEEEEECCEEEEEECCHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHCCCCCCCEEE
RWDGVRSLDHAMEMLRKQVAVTPPPQWVRVVGGFTEHQFAEKRLPTLEELNAAAPDTPVF
EECHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHCCCCCCCCEE
ILHLYDRALLNAAALRVVGYTKDTPNPPGGEIVRDAAGNPTGLLLAQPNATILYSTLAKG
EEEHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHCCCCCCEEEEECCCCEEEEHHHHCC
PKLPAEYQKNSTRHFMREVNRLGVTSVIDAGGGFQNYPDDYAVIEELHRDGQLSVRIAYN
CCCCHHHHCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEEEE
LFTQKPKQELADFSGWSKQVKPGQGDDLYRNNGAGEMLVYSAADFEDFQVARPDMPPNME
HHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCC
GDLEPVIRLLAENRWPWRLHATYDETISRALDVFEKVNKDVPLKGLNWFFDHAETITDRN
CCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
IDRIAALGGGIAVQHRMAFQGEYFVERYGAGRAERSPPIRRMLDAGIPVGAGTDATRVAS
HHHHHHHCCCEEEEEHHHHCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEC
YNPWVSLSWLVTGKTLGGLSLYPPSNLLDRESALRLWTEANTWFSSEPDKKGQVKAGQLA
CCCCEEEEEEEECCCCCCEEECCCCCHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
DLVVLSDDYFSVPEDRIQDITSVLTLLGGTPVHGDGDFKTLAPDLPPPMPDWSPVRTFGG
EEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCC
YQKRAETGERKYAFAAACGCATACGVHGHAHASALAAQVPAADERSFWGALGCACWAF
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PDRKLPDRKLPDRRQFIAAGLMAAAGLVPTSPKAQTMSETADLILFNGKVTTLDRTNPQ
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCEEEECCEEEEEECCCCC
AEAVAVKDGRILFAGTTADAMRHAGTLTKIIDVGGRRIIPGLIDSHMHIIRGGLNYNMEL
CEEEEEECCEEEEEECCHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHCCCCCCCEEE
RWDGVRSLDHAMEMLRKQVAVTPPPQWVRVVGGFTEHQFAEKRLPTLEELNAAAPDTPVF
EECHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHCCCCCCCCEE
ILHLYDRALLNAAALRVVGYTKDTPNPPGGEIVRDAAGNPTGLLLAQPNATILYSTLAKG
EEEHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHCCCCCCEEEEECCCCEEEEHHHHCC
PKLPAEYQKNSTRHFMREVNRLGVTSVIDAGGGFQNYPDDYAVIEELHRDGQLSVRIAYN
CCCCHHHHCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEEEE
LFTQKPKQELADFSGWSKQVKPGQGDDLYRNNGAGEMLVYSAADFEDFQVARPDMPPNME
HHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCC
GDLEPVIRLLAENRWPWRLHATYDETISRALDVFEKVNKDVPLKGLNWFFDHAETITDRN
CCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
IDRIAALGGGIAVQHRMAFQGEYFVERYGAGRAERSPPIRRMLDAGIPVGAGTDATRVAS
HHHHHHHCCCEEEEEHHHHCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEC
YNPWVSLSWLVTGKTLGGLSLYPPSNLLDRESALRLWTEANTWFSSEPDKKGQVKAGQLA
CCCCEEEEEEEECCCCCCEEECCCCCHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
DLVVLSDDYFSVPEDRIQDITSVLTLLGGTPVHGDGDFKTLAPDLPPPMPDWSPVRTFGG
EEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCC
YQKRAETGERKYAFAAACGCATACGVHGHAHASALAAQVPAADERSFWGALGCACWAF
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA