Click here to switch to the map view.

The map label for this gene is carA [H]

Identifier: 154245191

GI number: 154245191

Start: 1431209

End: 1432444

Strand: Direct

Name: carA [H]

Synonym: Xaut_1243

Alternate gene names: 154245191

Gene position: 1431209-1432444 (Clockwise)

Preceding gene: 154245189

Following gene: 154245193

Centisome position: 26.96

GC content: 68.2

Gene sequence:

>1236_bases
ATGACCAACGAACAAGACCACGCCCCGACCGCTCCGGCCGAAGGCCGGGATGTGCGCCCCTCCGCCGAAGGCTGGAGCGA
GCCCAAGCCCACCGCCGTTCTTGTCCTCGCGGACGGCACCGTCCTCGAAGGCTTTGGCATCGGCGCCGAAGCTCGCCTCG
CCGGCGAGGTGTGCTTCAACACCGCTATCACCGGCTATGAGGAGATCCTCACCGATCCCTCCTACGCTGGCCAGATCATC
ACCTTCACGTTCCCGCACATCGGCAACGTGGGGACCAACGAGGAAGACATCGAGACGGTGTCCATGGCCGCCGCCTCGGG
CGTCCGCGGCGTGGTGCTGCGCACCGCCGTCACCGAGCCGTCCAACTATCGCGCCACCCGCCACCTGAATGAATGGCTGA
AGGCGCGCGGCATCCCCGGCATCACCGGCATCGACACCCGCGCCCTCACCGCCCTCATCCGAGAGGCGGGCATGCCCAAC
GCGGTCATCGCCCATTCCGCGGACGGCGTCTTCGACATCGAGGCGCTGAAGGCCGAGGCGCGGGCCTGGCCAGGCCTCGT
CGGCATGGACCTTGTGCCCGGCGTCACCTCGGCCCAGCGTTTCGGCTGGGACGAGACCACCTGGGAATGGGAAAAGGGCT
ACGGGCACCAGGACGCGCCGCGTCACCACGTGGTGGCGGTGGACTATGGCGTGAAGCGCAACATTCTGCGCCTGCTCGCC
CGCGCCGGCTGCAAGGTGACGGTGGTTCCGGCCACCACCTCGGCCGACGACATCCTGGCCCTGAAGCCGGACGGCGTGTT
CCTCTCCAACGGCCCGGGCGATCCGGCGGCGACGGGGGAATATGCGGTGCCGGTGATCCGCCGGATCATCGAGACCGGGG
TGCCCACCTTCGGCATCTGCCTCGGCCACCAGATGATCGGCCTCGCGGTGGGCGGCCGCACGGTGAAGATGCACCAGGGC
CACCACGGCGCGAACCATCCGGTCAAGGACCTCACCACCGGCAAGGTGGAGATCACCTCCATGAACCACGGCTTCGCGGT
GGATCGCGAGACCCTGCCCAAGAATGCGGTGGAGACCCATGTCTCCCTGTTCGACGGCTCGAATGCCGGCATCGCGCTGG
CCGACCGGCCGGTGTTCTCGGTGCAGTACCACCCCGAGGCGAGCCCCGGCCCGCAGGACAGCCACTATCTGTTCGACCGC
TTCATCGAGATGATCGGCAAGCACAAGGCGGCCTGA

Upstream 100 bases:

>100_bases
GCACTTTCCACTCCGGTCCCTCGACCGTCCGGCGCCGCGCCGCACAAATTCGGTGCGGAACGCTGGAAACGCGACGCGCG
ACGCTCTATCTAGGCGATCC

Downstream 100 bases:

>100_bases
GAGCCTCTGAGACAAGAAAAAGGCCGGCGCATGCGCCGGCCTTTTTTGTTGCGCGCGTCGCCGGGCGCTCAGTCCAGGAA
ATTACCGTGCTCTGCCACAT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MTNEQDHAPTAPAEGRDVRPSAEGWSEPKPTAVLVLADGTVLEGFGIGAEARLAGEVCFNTAITGYEEILTDPSYAGQII
TFTFPHIGNVGTNEEDIETVSMAAASGVRGVVLRTAVTEPSNYRATRHLNEWLKARGIPGITGIDTRALTALIREAGMPN
AVIAHSADGVFDIEALKAEARAWPGLVGMDLVPGVTSAQRFGWDETTWEWEKGYGHQDAPRHHVVAVDYGVKRNILRLLA
RAGCKVTVVPATTSADDILALKPDGVFLSNGPGDPAATGEYAVPVIRRIIETGVPTFGICLGHQMIGLAVGGRTVKMHQG
HHGANHPVKDLTTGKVEITSMNHGFAVDRETLPKNAVETHVSLFDGSNAGIALADRPVFSVQYHPEASPGPQDSHYLFDR
FIEMIGKHKAA

Sequences:

>Translated_411_residues
MTNEQDHAPTAPAEGRDVRPSAEGWSEPKPTAVLVLADGTVLEGFGIGAEARLAGEVCFNTAITGYEEILTDPSYAGQII
TFTFPHIGNVGTNEEDIETVSMAAASGVRGVVLRTAVTEPSNYRATRHLNEWLKARGIPGITGIDTRALTALIREAGMPN
AVIAHSADGVFDIEALKAEARAWPGLVGMDLVPGVTSAQRFGWDETTWEWEKGYGHQDAPRHHVVAVDYGVKRNILRLLA
RAGCKVTVVPATTSADDILALKPDGVFLSNGPGDPAATGEYAVPVIRRIIETGVPTFGICLGHQMIGLAVGGRTVKMHQG
HHGANHPVKDLTTGKVEITSMNHGFAVDRETLPKNAVETHVSLFDGSNAGIALADRPVFSVQYHPEASPGPQDSHYLFDR
FIEMIGKHKAA
>Mature_410_residues
TNEQDHAPTAPAEGRDVRPSAEGWSEPKPTAVLVLADGTVLEGFGIGAEARLAGEVCFNTAITGYEEILTDPSYAGQIIT
FTFPHIGNVGTNEEDIETVSMAAASGVRGVVLRTAVTEPSNYRATRHLNEWLKARGIPGITGIDTRALTALIREAGMPNA
VIAHSADGVFDIEALKAEARAWPGLVGMDLVPGVTSAQRFGWDETTWEWEKGYGHQDAPRHHVVAVDYGVKRNILRLLAR
AGCKVTVVPATTSADDILALKPDGVFLSNGPGDPAATGEYAVPVIRRIIETGVPTFGICLGHQMIGLAVGGRTVKMHQGH
HGANHPVKDLTTGKVEITSMNHGFAVDRETLPKNAVETHVSLFDGSNAGIALADRPVFSVQYHPEASPGPQDSHYLFDRF
IEMIGKHKAA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=385, Percent_Identity=38.7012987012987, Blast_Score=248, Evalue=8e-66,
Organism=Homo sapiens, GI21361331, Length=409, Percent_Identity=34.9633251833741, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI169790915, Length=409, Percent_Identity=34.9633251833741, Blast_Score=226, Evalue=2e-59,
Organism=Escherichia coli, GI1786215, Length=384, Percent_Identity=51.8229166666667, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI193204318, Length=393, Percent_Identity=36.1323155216285, Blast_Score=222, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6322331, Length=420, Percent_Identity=35.4761904761905, Blast_Score=237, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6324878, Length=393, Percent_Identity=37.4045801526718, Blast_Score=236, Evalue=5e-63,
Organism=Drosophila melanogaster, GI45555749, Length=389, Percent_Identity=38.0462724935733, Blast_Score=235, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24642586, Length=389, Percent_Identity=38.0462724935733, Blast_Score=235, Evalue=5e-62,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 43879; Mature: 43748

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNEQDHAPTAPAEGRDVRPSAEGWSEPKPTAVLVLADGTVLEGFGIGAEARLAGEVCFN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEECCCCCCCHHHHHHHHHH
TAITGYEEILTDPSYAGQIITFTFPHIGNVGTNEEDIETVSMAAASGVRGVVLRTAVTEP
HHHHCHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCC
SNYRATRHLNEWLKARGIPGITGIDTRALTALIREAGMPNAVIAHSADGVFDIEALKAEA
CCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHH
RAWPGLVGMDLVPGVTSAQRFGWDETTWEWEKGYGHQDAPRHHVVAVDYGVKRNILRLLA
HHCCCCCCCHHCCCCCHHHHCCCCCCCEEHHCCCCCCCCCCCEEEEEECCHHHHHHHHHH
RAGCKVTVVPATTSADDILALKPDGVFLSNGPGDPAATGEYAVPVIRRIIETGVPTFGIC
HCCCEEEEEECCCCCCCEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHH
LGHQMIGLAVGGRTVKMHQGHHGANHPVKDLTTGKVEITSMNHGFAVDRETLPKNAVETH
HHHHEEEEEECCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEECHHHCCCHHHHHE
VSLFDGSNAGIALADRPVFSVQYHPEASPGPQDSHYLFDRFIEMIGKHKAA
EEEEECCCCCEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TNEQDHAPTAPAEGRDVRPSAEGWSEPKPTAVLVLADGTVLEGFGIGAEARLAGEVCFN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEECCCCCCCHHHHHHHHHH
TAITGYEEILTDPSYAGQIITFTFPHIGNVGTNEEDIETVSMAAASGVRGVVLRTAVTEP
HHHHCHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCC
SNYRATRHLNEWLKARGIPGITGIDTRALTALIREAGMPNAVIAHSADGVFDIEALKAEA
CCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHH
RAWPGLVGMDLVPGVTSAQRFGWDETTWEWEKGYGHQDAPRHHVVAVDYGVKRNILRLLA
HHCCCCCCCHHCCCCCHHHHCCCCCCCEEHHCCCCCCCCCCCEEEEEECCHHHHHHHHHH
RAGCKVTVVPATTSADDILALKPDGVFLSNGPGDPAATGEYAVPVIRRIIETGVPTFGIC
HCCCEEEEEECCCCCCCEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHH
LGHQMIGLAVGGRTVKMHQGHHGANHPVKDLTTGKVEITSMNHGFAVDRETLPKNAVETH
HHHHEEEEEECCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEECHHHCCCHHHHHE
VSLFDGSNAGIALADRPVFSVQYHPEASPGPQDSHYLFDRFIEMIGKHKAA
EEEEECCCCCEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]