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The map label for this gene is alsT [H]

Identifier: 154245189

GI number: 154245189

Start: 1429038

End: 1430453

Strand: Direct

Name: alsT [H]

Synonym: Xaut_1241

Alternate gene names: 154245189

Gene position: 1429038-1430453 (Clockwise)

Preceding gene: 154245185

Following gene: 154245191

Centisome position: 26.92

GC content: 66.67

Gene sequence:

>1416_bases
ATGGATGCACTGATCGACGTCCTGAACACAATTTTCTGGGGCTATGTCCTCATCTACGGCCTGCTGGCGGTGGGCGTGTA
TTTCACCTTCCGCCTGCGCCTTATCCAGATCCTGCATTTTCCGGAGATGTTGCGCTCCGTGCTGCGGGCGGATGCCACCG
ACAAGTCCGGTATCACGCCGTTTCAGGCCCTGTGCACCTCGCTTGCCTCGCGCGTGGGCACGGGCAACATCGCCGGCGTC
GCCGTGGCGCTCACCCTCGGCGGGCCGGGCGCCATTTTCTGGATGTGGGTGGTGGCCGCCCTCGGCATGGCGACCGCCTT
CGCCGAAAGCACGCTGGCCCAGCTCTACAAGGTGAAGGACGATGAGGGCCGCTATCGCGGCGGGCCGGCCTTCTATATCG
CGCGCGGCCTGAAGGCGCCGTGGGCCGGCGGTGTGTTCTCGGTCTGCCTCATCCTCTCCTTCGGCCTGGTCTTCAACGCC
GTGCAGGCCAATTCCATCGCCGATGCCATGCACGGCGCCTTTGAAATCCCCAAGCTCTGGACCGGCCTGGCCGTCGCCGC
GCTCTCGGGAATCATCATCTTCGGCGGCATCGCCGCCATCGCGCGCACCGCAGAGCTGGTGGTGCCGTTCATGGCGCTCG
CCTATGTGGCCCTCGCGCTCTATGCGCTCGTGACCCATCTCGGCGCGGTGCCGGCCATGCTCGGCCATATCGTCGGCAGC
GCCTTTGGCCTTGAGCCGGCGGCAGGCGGCGTTTCGGGGGCGGTGGCCGCGGCCATGCTCAACGGGGTGAAGCGCGGCCT
GTTCTCCAACGAGGCCGGCATGGGCAGCGCGCCCAATATCGCCGCCGTGGCGACGCCCGATCCGCATCACCCCGTGAGCC
AGGGCTTCGTGCAGTCCCTGGGGGTGTTCATCGACACCATCGTGATCTGCACCGCCACCGCGCTGCTGATCCTGCTGTCG
GGCGTGGAGCTGGGGTCGGCCACCGGAACGCAGCTCACCCAGGCGGCGCTGGAAGCCCATGTGGGTTGGCTCGGGCGCTA
TTTCGTGGCCATCGCCATCTTCTTCTTCGCCTTCACCTCCATCATCGGCAACTACTCCTATGCGGAGAATGCACTGGTGT
TCCTCGGCCACGGTCATGTGTCCGGGGTGGTGCTCCTGCGCATCGGCGTGCTCGCCATGGTGGTGTGGGGCGCGGTGCAG
AGCGTCGCCACCGTGTTCAATGCGGCCGACGCCTCCATGGGGCTCATGGCCTCCATCAATCTGGTGGCCATCGTGCTGCT
GTCGGGCACGGTGGTGCGGCTAGCGCAGGATTATCTCGTCAAGCGCAAGGCCGGTGTGGAGCCGGTCTTCATCGCCACCG
ACATGCCGGACCTGCCGCCAGGCGTGGACGGGGAAATCTGGAAGCGCCGGGGCTGA

Upstream 100 bases:

>100_bases
GGCGGAACGGTACTTTTGGGTGCGGATGCGTGTGGACCCTGTGGCACGCCGTCGCTAGCCTGAGCGATGCACGTGGTCGT
TGCACGGGGGCTGGGGCGCA

Downstream 100 bases:

>100_bases
CCTGGCGCTGCCTTCCGGGAAGAGAAGGCGGAAGCCGGCGGGCCTGACCCGCCGGCAAAACTGGCTCAGGCCGCGGCGGT
GAGGGCTTCCTTCACCCAGC

Product: amino acid carrier protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MDALIDVLNTIFWGYVLIYGLLAVGVYFTFRLRLIQILHFPEMLRSVLRADATDKSGITPFQALCTSLASRVGTGNIAGV
AVALTLGGPGAIFWMWVVAALGMATAFAESTLAQLYKVKDDEGRYRGGPAFYIARGLKAPWAGGVFSVCLILSFGLVFNA
VQANSIADAMHGAFEIPKLWTGLAVAALSGIIIFGGIAAIARTAELVVPFMALAYVALALYALVTHLGAVPAMLGHIVGS
AFGLEPAAGGVSGAVAAAMLNGVKRGLFSNEAGMGSAPNIAAVATPDPHHPVSQGFVQSLGVFIDTIVICTATALLILLS
GVELGSATGTQLTQAALEAHVGWLGRYFVAIAIFFFAFTSIIGNYSYAENALVFLGHGHVSGVVLLRIGVLAMVVWGAVQ
SVATVFNAADASMGLMASINLVAIVLLSGTVVRLAQDYLVKRKAGVEPVFIATDMPDLPPGVDGEIWKRRG

Sequences:

>Translated_471_residues
MDALIDVLNTIFWGYVLIYGLLAVGVYFTFRLRLIQILHFPEMLRSVLRADATDKSGITPFQALCTSLASRVGTGNIAGV
AVALTLGGPGAIFWMWVVAALGMATAFAESTLAQLYKVKDDEGRYRGGPAFYIARGLKAPWAGGVFSVCLILSFGLVFNA
VQANSIADAMHGAFEIPKLWTGLAVAALSGIIIFGGIAAIARTAELVVPFMALAYVALALYALVTHLGAVPAMLGHIVGS
AFGLEPAAGGVSGAVAAAMLNGVKRGLFSNEAGMGSAPNIAAVATPDPHHPVSQGFVQSLGVFIDTIVICTATALLILLS
GVELGSATGTQLTQAALEAHVGWLGRYFVAIAIFFFAFTSIIGNYSYAENALVFLGHGHVSGVVLLRIGVLAMVVWGAVQ
SVATVFNAADASMGLMASINLVAIVLLSGTVVRLAQDYLVKRKAGVEPVFIATDMPDLPPGVDGEIWKRRG
>Mature_471_residues
MDALIDVLNTIFWGYVLIYGLLAVGVYFTFRLRLIQILHFPEMLRSVLRADATDKSGITPFQALCTSLASRVGTGNIAGV
AVALTLGGPGAIFWMWVVAALGMATAFAESTLAQLYKVKDDEGRYRGGPAFYIARGLKAPWAGGVFSVCLILSFGLVFNA
VQANSIADAMHGAFEIPKLWTGLAVAALSGIIIFGGIAAIARTAELVVPFMALAYVALALYALVTHLGAVPAMLGHIVGS
AFGLEPAAGGVSGAVAAAMLNGVKRGLFSNEAGMGSAPNIAAVATPDPHHPVSQGFVQSLGVFIDTIVICTATALLILLS
GVELGSATGTQLTQAALEAHVGWLGRYFVAIAIFFFAFTSIIGNYSYAENALVFLGHGHVSGVVLLRIGVLAMVVWGAVQ
SVATVFNAADASMGLMASINLVAIVLLSGTVVRLAQDYLVKRKAGVEPVFIATDMPDLPPGVDGEIWKRRG

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=471, Percent_Identity=45.0106157112527, Blast_Score=375, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 49071; Mature: 49071

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALIDVLNTIFWGYVLIYGLLAVGVYFTFRLRLIQILHFPEMLRSVLRADATDKSGITP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
FQALCTSLASRVGTGNIAGVAVALTLGGPGAIFWMWVVAALGMATAFAESTLAQLYKVKD
HHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DEGRYRGGPAFYIARGLKAPWAGGVFSVCLILSFGLVFNAVQANSIADAMHGAFEIPKLW
CCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
TGLAVAALSGIIIFGGIAAIARTAELVVPFMALAYVALALYALVTHLGAVPAMLGHIVGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGLEPAAGGVSGAVAAAMLNGVKRGLFSNEAGMGSAPNIAAVATPDPHHPVSQGFVQSL
HCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHH
GVFIDTIVICTATALLILLSGVELGSATGTQLTQAALEAHVGWLGRYFVAIAIFFFAFTS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGNYSYAENALVFLGHGHVSGVVLLRIGVLAMVVWGAVQSVATVFNAADASMGLMASIN
HHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVAIVLLSGTVVRLAQDYLVKRKAGVEPVFIATDMPDLPPGVDGEIWKRRG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHCCCC
>Mature Secondary Structure
MDALIDVLNTIFWGYVLIYGLLAVGVYFTFRLRLIQILHFPEMLRSVLRADATDKSGITP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
FQALCTSLASRVGTGNIAGVAVALTLGGPGAIFWMWVVAALGMATAFAESTLAQLYKVKD
HHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DEGRYRGGPAFYIARGLKAPWAGGVFSVCLILSFGLVFNAVQANSIADAMHGAFEIPKLW
CCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
TGLAVAALSGIIIFGGIAAIARTAELVVPFMALAYVALALYALVTHLGAVPAMLGHIVGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGLEPAAGGVSGAVAAAMLNGVKRGLFSNEAGMGSAPNIAAVATPDPHHPVSQGFVQSL
HCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHH
GVFIDTIVICTATALLILLSGVELGSATGTQLTQAALEAHVGWLGRYFVAIAIFFFAFTS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGNYSYAENALVFLGHGHVSGVVLLRIGVLAMVVWGAVQSVATVFNAADASMGLMASIN
HHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVAIVLLSGTVVRLAQDYLVKRKAGVEPVFIATDMPDLPPGVDGEIWKRRG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969507; 9384377; 7710280 [H]