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The map label for this gene is nadB [H]

Identifier: 154245130

GI number: 154245130

Start: 1358485

End: 1360026

Strand: Reverse

Name: nadB [H]

Synonym: Xaut_1182

Alternate gene names: 154245130

Gene position: 1360026-1358485 (Counterclockwise)

Preceding gene: 154245131

Following gene: 154245129

Centisome position: 25.62

GC content: 76.72

Gene sequence:

>1542_bases
GTGACCGGCTTCGAGCGGCTGGAGGGCCGCCCCGTCATCGTTGGCGGCGGCATCGCCGGGCTTGCCGCCGCCCTGTTCCT
CTCGCCCGAGCCGGTGGTGCTGGTCACCCGCGCCCCCCTCGGCGCGGACGGCTCCAGCCCGCTGGCCCAGGGCGGCATGG
CCGCCAGCGTCGGCACAGACGACGATGCCGACCTCCACCTCGCCGACACGCTCATGGCCGGCGACGGCCTGTGCGACCCC
GAGGTCGCCCGCCGCATCCTCGCCGATGGCCCTGCCGCCGTGGCGGAGCTGGAACGTCTCGGCGTCGCCTTCGACCGGGC
CGCCGACGGCAGCCTCGCACTCGGCCTTGAGGCCGCCCACAGCCGGCGCCGCATCGTCCATGCGGACGGCGACGCCACCG
GCCGCGCGCTGGTGCAGGCCCTCACCATCGCCGCCCGCGCCTGCCCGTCCATCACCGTGGTGGAGGCCGAAGCCCGGCGC
ATCCTGATGGAGGATGGCGCAGTAGCCGGGCTGCTGGTGTCGGGGCGAGACGGCGGCGCCGTGCTGCGCACCCGCCGCCT
CATCCTGGCGACGGGGGGCGTGGGCGCCCTGTTCGAGCACACCACCAATCCCGCCTCCTCCTTCGGCATGGGCCTCGCGC
TGGCCGCCGAGGCCGGGGCGGAGCTGGCGGACCTCGAATTCGTGCAGTTCCACCCCACCGCGCTCGCCACCTCGGCCCGC
CCGCTCTCCCTCATCAGCGAGGCGGTGCGCGGCGAAGGCGCCCTCATCGTGGATCGCGCCGGCCGCCGCGTGCTGGAAGC
CGTGCCGGGCGCCGAGCTCGCCCCGCGCGACGTGGTCGCCCGCGCCGTGTGGCAGGAGCTGGTTGCGGGGCGCGAGGTGT
TCCTCGATGCCCGTGCCAGCCTCGGCGCGCGCTTTGCCACACGCTTTCCCTCGGTCGCCGCCGGCTGCCGGGCGGCGGGC
ATCGATCCGGCTACCGACCTCGTGCCGATCCGCCCGGCGGCGCACTACCACATGGGCGGCATCGGCGTGGATGCGGACGG
CCGCAGCTCCGTTCCCGGCCTGTGGGCCGCGGGCGAGGTGGCGGCCACCGGCCTCCACGGCGCCAACCGGCTCGCCAGCA
ATTCGCTTCTGGAGGCGGTGGTCTGCGGACGCGCGGCGGCCCTGAGCGTGGCCGGAACGCCGGCCCGCGCCCCCGCCCGC
CGAATGGCCGTTCATTTGCCGGCGGCGCCGGACGCGACCTCAGTCCGCCCCATCGTCTCGCGCCATCTCGGCGTCACCCG
CGATGCCGCCGGCCTTGCCACCGCCATCGCCCGCCTGCTGCCGCTGGCAGAGGGCGCGGGGCCGGCCTGCGCCCCCGCCC
GGGTGGGGCTCATGATGGCGGTGTCGGCGCTGGCCCGCGAAGAAAGCCGGGGCGCCCATGCGCGCACGGATTTCCCCGCG
CCCGCCGCCGTGGCCCGGCGCTCCCGCACCACCCTTGAGCGCACCCTGGCCGAGGCCCGCCGCCATGCCGCGCCGGCCTT
CGCCCTCGCCTCATCCAGCTGA

Upstream 100 bases:

>100_bases
CCAACATCCGCAAGGCGCTGGAAGGCAACGTGCACGAAGTGACCATCGCGGCGGACGTGGCGGATCGCGCCCGCGCCTCG
GTGGAGCGGATGCTGGCGCT

Downstream 100 bases:

>100_bases
CGGATCGTTCCATGCCCCTCGACCCGCTCCCCGAGCTGATGGTGGAGCCCATCGTGCGCGCCGCGCTGCTGGAGGATCTC
GGCCGGGCCGGCGACATCAC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MTGFERLEGRPVIVGGGIAGLAAALFLSPEPVVLVTRAPLGADGSSPLAQGGMAASVGTDDDADLHLADTLMAGDGLCDP
EVARRILADGPAAVAELERLGVAFDRAADGSLALGLEAAHSRRRIVHADGDATGRALVQALTIAARACPSITVVEAEARR
ILMEDGAVAGLLVSGRDGGAVLRTRRLILATGGVGALFEHTTNPASSFGMGLALAAEAGAELADLEFVQFHPTALATSAR
PLSLISEAVRGEGALIVDRAGRRVLEAVPGAELAPRDVVARAVWQELVAGREVFLDARASLGARFATRFPSVAAGCRAAG
IDPATDLVPIRPAAHYHMGGIGVDADGRSSVPGLWAAGEVAATGLHGANRLASNSLLEAVVCGRAAALSVAGTPARAPAR
RMAVHLPAAPDATSVRPIVSRHLGVTRDAAGLATAIARLLPLAEGAGPACAPARVGLMMAVSALAREESRGAHARTDFPA
PAAVARRSRTTLERTLAEARRHAAPAFALASSS

Sequences:

>Translated_513_residues
MTGFERLEGRPVIVGGGIAGLAAALFLSPEPVVLVTRAPLGADGSSPLAQGGMAASVGTDDDADLHLADTLMAGDGLCDP
EVARRILADGPAAVAELERLGVAFDRAADGSLALGLEAAHSRRRIVHADGDATGRALVQALTIAARACPSITVVEAEARR
ILMEDGAVAGLLVSGRDGGAVLRTRRLILATGGVGALFEHTTNPASSFGMGLALAAEAGAELADLEFVQFHPTALATSAR
PLSLISEAVRGEGALIVDRAGRRVLEAVPGAELAPRDVVARAVWQELVAGREVFLDARASLGARFATRFPSVAAGCRAAG
IDPATDLVPIRPAAHYHMGGIGVDADGRSSVPGLWAAGEVAATGLHGANRLASNSLLEAVVCGRAAALSVAGTPARAPAR
RMAVHLPAAPDATSVRPIVSRHLGVTRDAAGLATAIARLLPLAEGAGPACAPARVGLMMAVSALAREESRGAHARTDFPA
PAAVARRSRTTLERTLAEARRHAAPAFALASSS
>Mature_512_residues
TGFERLEGRPVIVGGGIAGLAAALFLSPEPVVLVTRAPLGADGSSPLAQGGMAASVGTDDDADLHLADTLMAGDGLCDPE
VARRILADGPAAVAELERLGVAFDRAADGSLALGLEAAHSRRRIVHADGDATGRALVQALTIAARACPSITVVEAEARRI
LMEDGAVAGLLVSGRDGGAVLRTRRLILATGGVGALFEHTTNPASSFGMGLALAAEAGAELADLEFVQFHPTALATSARP
LSLISEAVRGEGALIVDRAGRRVLEAVPGAELAPRDVVARAVWQELVAGREVFLDARASLGARFATRFPSVAAGCRAAGI
DPATDLVPIRPAAHYHMGGIGVDADGRSSVPGLWAAGEVAATGLHGANRLASNSLLEAVVCGRAAALSVAGTPARAPARR
MAVHLPAAPDATSVRPIVSRHLGVTRDAAGLATAIARLLPLAEGAGPACAPARVGLMMAVSALAREESRGAHARTDFPAP
AAVARRSRTTLERTLAEARRHAAPAFALASSS

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=375, Percent_Identity=35.2, Blast_Score=192, Evalue=8e-49,
Organism=Escherichia coli, GI1788928, Length=514, Percent_Identity=36.1867704280156, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI1790597, Length=518, Percent_Identity=31.6602316602317, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1786942, Length=504, Percent_Identity=31.3492063492063, Blast_Score=181, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17550100, Length=526, Percent_Identity=30.4182509505703, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17505833, Length=502, Percent_Identity=29.6812749003984, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71986328, Length=438, Percent_Identity=28.9954337899543, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322701, Length=529, Percent_Identity=30.2457466918715, Blast_Score=190, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6322416, Length=425, Percent_Identity=32.7058823529412, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6320788, Length=475, Percent_Identity=27.3684210526316, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322511, Length=475, Percent_Identity=25.2631578947368, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI17137288, Length=500, Percent_Identity=31.6, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24655642, Length=500, Percent_Identity=31.6, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24655647, Length=500, Percent_Identity=31.6, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24663005, Length=504, Percent_Identity=29.5634920634921, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 51995; Mature: 51864

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGFERLEGRPVIVGGGIAGLAAALFLSPEPVVLVTRAPLGADGSSPLAQGGMAASVGTD
CCCCHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHCCCEEEECCCC
DDADLHLADTLMAGDGLCDPEVARRILADGPAAVAELERLGVAFDRAADGSLALGLEAAH
CCCCEEHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHCHHHCCCCCCCEEEEHHHHH
SRRRIVHADGDATGRALVQALTIAARACPSITVVEAEARRILMEDGAVAGLLVSGRDGGA
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCEEEEEEECCCCCH
VLRTRRLILATGGVGALFEHTTNPASSFGMGLALAAEAGAELADLEFVQFHPTALATSAR
HHEEEEEEEEECCCHHHHHCCCCCHHHHCCCEEEHHHCCCCHHCCEEEEECCHHHHCCCC
PLSLISEAVRGEGALIVDRAGRRVLEAVPGAELAPRDVVARAVWQELVAGREVFLDARAS
HHHHHHHHHCCCCEEEEEHHCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHEEHHHHH
LGARFATRFPSVAAGCRAAGIDPATDLVPIRPAAHYHMGGIGVDADGRSSVPGLWAAGEV
HHHHHHHHCCHHHHCCHHCCCCCCCCEEECCCCCCEECCCEEECCCCCCCCCCCEECCHH
AATGLHGANRLASNSLLEAVVCGRAAALSVAGTPARAPARRMAVHLPAAPDATSVRPIVS
HHHCCCHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCHHHEEEECCCCCCCCCHHHHHH
RHLGVTRDAAGLATAIARLLPLAEGAGPACAPARVGLMMAVSALAREESRGAHARTDFPA
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PAAVARRSRTTLERTLAEARRHAAPAFALASSS
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
>Mature Secondary Structure 
TGFERLEGRPVIVGGGIAGLAAALFLSPEPVVLVTRAPLGADGSSPLAQGGMAASVGTD
CCCHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHCCCEEEECCCC
DDADLHLADTLMAGDGLCDPEVARRILADGPAAVAELERLGVAFDRAADGSLALGLEAAH
CCCCEEHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHCHHHCCCCCCCEEEEHHHHH
SRRRIVHADGDATGRALVQALTIAARACPSITVVEAEARRILMEDGAVAGLLVSGRDGGA
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCEEEEEEECCCCCH
VLRTRRLILATGGVGALFEHTTNPASSFGMGLALAAEAGAELADLEFVQFHPTALATSAR
HHEEEEEEEEECCCHHHHHCCCCCHHHHCCCEEEHHHCCCCHHCCEEEEECCHHHHCCCC
PLSLISEAVRGEGALIVDRAGRRVLEAVPGAELAPRDVVARAVWQELVAGREVFLDARAS
HHHHHHHHHCCCCEEEEEHHCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHEEHHHHH
LGARFATRFPSVAAGCRAAGIDPATDLVPIRPAAHYHMGGIGVDADGRSSVPGLWAAGEV
HHHHHHHHCCHHHHCCHHCCCCCCCCEEECCCCCCEECCCEEECCCCCCCCCCCEECCHH
AATGLHGANRLASNSLLEAVVCGRAAALSVAGTPARAPARRMAVHLPAAPDATSVRPIVS
HHHCCCHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCHHHEEEECCCCCCCCCHHHHHH
RHLGVTRDAAGLATAIARLLPLAEGAGPACAPARVGLMMAVSALAREESRGAHARTDFPA
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PAAVARRSRTTLERTLAEARRHAAPAFALASSS
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]