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The map label for this gene is purR [H]

Identifier: 154245125

GI number: 154245125

Start: 1352965

End: 1354047

Strand: Direct

Name: purR [H]

Synonym: Xaut_1177

Alternate gene names: 154245125

Gene position: 1352965-1354047 (Clockwise)

Preceding gene: 154245123

Following gene: 154245126

Centisome position: 25.48

GC content: 70.73

Gene sequence:

>1083_bases
ATGGATGACAATATCCCCGCTCAGGCCCCAGCCGGCCAGCTGCCCGCTTCCATCGGCAAGGTGACGCTCCAGGGCCTCGC
CCGCGAGCTTGGATTGTCCACTGCCACCATTTCGCTCGCCCTGCGCGACAGCCCCATGGTGGCGGAGGCGACGCGCCTCA
AGGTGCAGGAAGTGGCCCGCGCCCGTGGCTATGTGGCCAATCGCGGCGCCGCGGCCCTGCGCACGGCGCGCACCAACATC
GTGGCGCTGGGCCTGCACGACATCGTCAACCCCTCCTTCACCGAGCTGCTCGCGGCGGTGGAGGAGGCGCTGACCGCCGC
TGGCAAGACCGTGCTGCTCGGGGTCAGCCAGGAGGATGTGGCGCGCCAGACCCGCACCCTGGGGACCCTTGCCGAATATC
GCCCCGACGCCTTCCTGGTCTCGCCCGCCGCTTATTCCACGGTCGCGGACCTGAAGGCGCTCGCCTCCACCGGCATCGCC
GTGGTGCAGGTGACGCGGGAGATCGAGGGCTCCGGTTTCGATTTCGCCGGCTCGGACGATGTGACCGGGGTGGCGCTGGG
CGTCGCCCATCTGGTGGCGCTGGGCCATACCCGCATCGGCATGCTGGGCGGCTTCGGCGCCATCTCCACCGGCCGCAAGC
GGCTCGCTGGATATAAGGCGGGTCTCGCCGCCGCCGGCCTCACCTTCGATCCCACCCTGGTGATGGAAGGGCCGGGCCTG
CGGGAAGAAGGCCGCCGCCAGCTGGCGCGCCTGCTGGCGCTGGAGGAGCCGCCCACCGCCGCCGTCAGCTTCAACGACCT
CTCCGCCTTCGGTGCCCTTATGGAGCTGCAGGCGGCCGGGCTCACGGTGGGCCGCGACTTCTCGCTGGTGGGCTATGACG
ATATCGGCGAGGCCAAGGTCTGGCATCCGGGGCTGACCTCGGTGCACACCTTCATCCCCGAATATGGCCGGGCCGCCGCC
GACCTCGCCCTGAAGCGCATCGCTGATCCGTCGCGCCCGGTGGAACGGGTGGAGATGCCCCCCAAGCTGGTGGTGCGCGC
CACCACAGCCCCGCCGGTGCCGGTGAAGCGCCGGCCCCTATGA

Upstream 100 bases:

>100_bases
TCGTCCCCGTGCCAATTACCATTGGTCGTTGCCGTTAAATCGTTTAACATGAACGAAAGGACGGCGCGAGACGCGACAGA
TCCGGTTATAGGGAAGGCCC

Downstream 100 bases:

>100_bases
TCCTTCCGATTGCTGAGGCCGACGATCCGCGCATCGCGGATTTCCGGGACGTGCGGGAGCGCGACCTCGTGGGCCGGCAG
GGCGCCTTCATGGCCGAGGG

Product: periplasmic binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MDDNIPAQAPAGQLPASIGKVTLQGLARELGLSTATISLALRDSPMVAEATRLKVQEVARARGYVANRGAAALRTARTNI
VALGLHDIVNPSFTELLAAVEEALTAAGKTVLLGVSQEDVARQTRTLGTLAEYRPDAFLVSPAAYSTVADLKALASTGIA
VVQVTREIEGSGFDFAGSDDVTGVALGVAHLVALGHTRIGMLGGFGAISTGRKRLAGYKAGLAAAGLTFDPTLVMEGPGL
REEGRRQLARLLALEEPPTAAVSFNDLSAFGALMELQAAGLTVGRDFSLVGYDDIGEAKVWHPGLTSVHTFIPEYGRAAA
DLALKRIADPSRPVERVEMPPKLVVRATTAPPVPVKRRPL

Sequences:

>Translated_360_residues
MDDNIPAQAPAGQLPASIGKVTLQGLARELGLSTATISLALRDSPMVAEATRLKVQEVARARGYVANRGAAALRTARTNI
VALGLHDIVNPSFTELLAAVEEALTAAGKTVLLGVSQEDVARQTRTLGTLAEYRPDAFLVSPAAYSTVADLKALASTGIA
VVQVTREIEGSGFDFAGSDDVTGVALGVAHLVALGHTRIGMLGGFGAISTGRKRLAGYKAGLAAAGLTFDPTLVMEGPGL
REEGRRQLARLLALEEPPTAAVSFNDLSAFGALMELQAAGLTVGRDFSLVGYDDIGEAKVWHPGLTSVHTFIPEYGRAAA
DLALKRIADPSRPVERVEMPPKLVVRATTAPPVPVKRRPL
>Mature_360_residues
MDDNIPAQAPAGQLPASIGKVTLQGLARELGLSTATISLALRDSPMVAEATRLKVQEVARARGYVANRGAAALRTARTNI
VALGLHDIVNPSFTELLAAVEEALTAAGKTVLLGVSQEDVARQTRTLGTLAEYRPDAFLVSPAAYSTVADLKALASTGIA
VVQVTREIEGSGFDFAGSDDVTGVALGVAHLVALGHTRIGMLGGFGAISTGRKRLAGYKAGLAAAGLTFDPTLVMEGPGL
REEGRRQLARLLALEEPPTAAVSFNDLSAFGALMELQAAGLTVGRDFSLVGYDDIGEAKVWHPGLTSVHTFIPEYGRAAA
DLALKRIADPSRPVERVEMPPKLVVRATTAPPVPVKRRPL

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=30.3303303303303, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790194, Length=328, Percent_Identity=28.0487804878049, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1787948, Length=331, Percent_Identity=27.190332326284, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790369, Length=312, Percent_Identity=28.2051282051282, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1788474, Length=330, Percent_Identity=27.2727272727273, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787906, Length=339, Percent_Identity=27.4336283185841, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=26.865671641791, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1789068, Length=297, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI48994940, Length=309, Percent_Identity=28.1553398058252, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37596; Mature: 37596

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDNIPAQAPAGQLPASIGKVTLQGLARELGLSTATISLALRDSPMVAEATRLKVQEVAR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
ARGYVANRGAAALRTARTNIVALGLHDIVNPSFTELLAAVEEALTAAGKTVLLGVSQEDV
HHCCCCCCCHHHHHHHCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHH
ARQTRTLGTLAEYRPDAFLVSPAAYSTVADLKALASTGIAVVQVTREIEGSGFDFAGSDD
HHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHCCEEEEEEEHHHCCCCCCCCCCCC
VTGVALGVAHLVALGHTRIGMLGGFGAISTGRKRLAGYKAGLAAAGLTFDPTLVMEGPGL
CHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
REEGRRQLARLLALEEPPTAAVSFNDLSAFGALMELQAAGLTVGRDFSLVGYDDIGEAKV
CHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHCCEEECCCEEEECCCCCCCCEE
WHPGLTSVHTFIPEYGRAAADLALKRIADPSRPVERVEMPPKLVVRATTAPPVPVKRRPL
CCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MDDNIPAQAPAGQLPASIGKVTLQGLARELGLSTATISLALRDSPMVAEATRLKVQEVAR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
ARGYVANRGAAALRTARTNIVALGLHDIVNPSFTELLAAVEEALTAAGKTVLLGVSQEDV
HHCCCCCCCHHHHHHHCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHH
ARQTRTLGTLAEYRPDAFLVSPAAYSTVADLKALASTGIAVVQVTREIEGSGFDFAGSDD
HHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHCCEEEEEEEHHHCCCCCCCCCCCC
VTGVALGVAHLVALGHTRIGMLGGFGAISTGRKRLAGYKAGLAAAGLTFDPTLVMEGPGL
CHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
REEGRRQLARLLALEEPPTAAVSFNDLSAFGALMELQAAGLTVGRDFSLVGYDDIGEAKV
CHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHCCEEECCCEEEECCCCCCCCEE
WHPGLTSVHTFIPEYGRAAADLALKRIADPSRPVERVEMPPKLVVRATTAPPVPVKRRPL
CCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA