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The map label for this gene is smeIM [H]

Identifier: 154245121

GI number: 154245121

Start: 1349195

End: 1350364

Strand: Reverse

Name: smeIM [H]

Synonym: Xaut_1173

Alternate gene names: 154245121

Gene position: 1350364-1349195 (Counterclockwise)

Preceding gene: 154245124

Following gene: 154245119

Centisome position: 25.44

GC content: 70.51

Gene sequence:

>1170_bases
GTGCCCGCGGCGGCACCCCGTGCCGCGCGCGCCATGTCTTCGGCGAAGCGGATGCGGGAGGCGGCCGGCGCCGCGCCGGG
CGACCTGCCCCTCGACCAGATCCTCCTCGGCGACAGCATCCAGATGATGGCCGGCCTGCCCGCCGGCAGCGTGGACCTCG
TGTTCGCCGATCCGCCCTACAATCTCCAGCTCGGCGGTGAGCTGAAGCGGCCCGACGAGAGCCTCGTCGATGCCGTGGAC
GACGACTGGGACCGCTTCGACAGCTTCGCCACCTATGATGCCTTCACCCGCGCCTGGCTTCTGGCCGCGCGGCGGGCGCT
GAAGCCCAACGGCACGCTGTTCGTCATCGGTTCCTATCACAACATCTTCCGCGTCGGCGCCATGATGCAGGATCTGGGCT
TCTGGATCCTCAACGACATCGTGTGGCGCAAGGCCAACCCCATGCCCAACTTCCGGGGCAGGCGCTTCACCAATGCCCAC
GAGACCCTGATCTGGGCCGCCCGCAGCGCGGATGCCAAATACACCTTCAATTACGAGGCGCTGAAAGCCGGCAACGAAGA
CGTGCAGGTGCGCTCGGACTGGCTGTTTCCCCTCTGCACCGGCCAGGAGCGGCTGAAGGACGGGGCGGGAAAGAAGCTGC
ACCCCACCCAGAAGCCCGAGGCCCTGCTCGCCCGCGTCCTGCTCTCGGCGACGAAGCCCGGCGACGTGGTGCTCGACCCC
TTCTTCGGCTCCGGCACCACCGGCGCTGTGGCGAAGCGCCTGCGCCGCTCCTTCATCGGCATCGAGCGGGAGGAGGCCTA
TGCGCAGGCCGCGCGCGCCCGCATCGACGCGGTGGAGCCGCTGCCGGAAGATGCCCTCGTGGCCCCGCCCAGCGCGCGGG
AAGCCCCGCGCGTCGCCTTCTCGGTGCTGGTGGAGCGCGGGCTGGTGACGCCCGGCGCCGAGCTGACCGACGCCAAGGGC
CGCTTCCGCGCCGTGGTGCGGGCCGATGGCACATTGGCACTGGCCGGCGGGCAGAATGTGGGCTCCATCCACCGCGTCGG
CGCGCTGGCGCAGGGGGCGGAAGCCTGCAACGGCTGGACCTTCTGGCACGTGGAAGAACAGGGCCGCCGCCATCCCATCG
ACACCCTGCGCGCTCGCCTCAGGGCCGAGATGGGCATCGCGGCGGAATAG

Upstream 100 bases:

>100_bases
GGCGCGCCAGTTGGGCGTTTGTCCGGAGTATGCCGAGCCTGCCCCCTTGGCGGCCCCGCGGGCCGCGAACGCGGTCGCTT
CGGCCCCGCGGGCCGTCGAT

Downstream 100 bases:

>100_bases
GCCAGCCGGGACGCTCCACTTACGCACGCCGGTCACGCCCGCCTATCCCGGCCGCAGCATCCCCTCTTCAAAGCCCGGCC
ACGGATGCCTGATGGACCTC

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.SmeI; Adenine-specific methyltransferase SmeIP; M.CcrMI ortholog; Modification methylase SmeIP; M.SmeIP [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MPAAAPRAARAMSSAKRMREAAGAAPGDLPLDQILLGDSIQMMAGLPAGSVDLVFADPPYNLQLGGELKRPDESLVDAVD
DDWDRFDSFATYDAFTRAWLLAARRALKPNGTLFVIGSYHNIFRVGAMMQDLGFWILNDIVWRKANPMPNFRGRRFTNAH
ETLIWAARSADAKYTFNYEALKAGNEDVQVRSDWLFPLCTGQERLKDGAGKKLHPTQKPEALLARVLLSATKPGDVVLDP
FFGSGTTGAVAKRLRRSFIGIEREEAYAQAARARIDAVEPLPEDALVAPPSAREAPRVAFSVLVERGLVTPGAELTDAKG
RFRAVVRADGTLALAGGQNVGSIHRVGALAQGAEACNGWTFWHVEEQGRRHPIDTLRARLRAEMGIAAE

Sequences:

>Translated_389_residues
MPAAAPRAARAMSSAKRMREAAGAAPGDLPLDQILLGDSIQMMAGLPAGSVDLVFADPPYNLQLGGELKRPDESLVDAVD
DDWDRFDSFATYDAFTRAWLLAARRALKPNGTLFVIGSYHNIFRVGAMMQDLGFWILNDIVWRKANPMPNFRGRRFTNAH
ETLIWAARSADAKYTFNYEALKAGNEDVQVRSDWLFPLCTGQERLKDGAGKKLHPTQKPEALLARVLLSATKPGDVVLDP
FFGSGTTGAVAKRLRRSFIGIEREEAYAQAARARIDAVEPLPEDALVAPPSAREAPRVAFSVLVERGLVTPGAELTDAKG
RFRAVVRADGTLALAGGQNVGSIHRVGALAQGAEACNGWTFWHVEEQGRRHPIDTLRARLRAEMGIAAE
>Mature_388_residues
PAAAPRAARAMSSAKRMREAAGAAPGDLPLDQILLGDSIQMMAGLPAGSVDLVFADPPYNLQLGGELKRPDESLVDAVDD
DWDRFDSFATYDAFTRAWLLAARRALKPNGTLFVIGSYHNIFRVGAMMQDLGFWILNDIVWRKANPMPNFRGRRFTNAHE
TLIWAARSADAKYTFNYEALKAGNEDVQVRSDWLFPLCTGQERLKDGAGKKLHPTQKPEALLARVLLSATKPGDVVLDPF
FGSGTTGAVAKRLRRSFIGIEREEAYAQAARARIDAVEPLPEDALVAPPSAREAPRVAFSVLVERGLVTPGAELTDAKGR
FRAVVRADGTLALAGGQNVGSIHRVGALAQGAEACNGWTFWHVEEQGRRHPIDTLRARLRAEMGIAAE

Specific function: This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication an

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=264, Percent_Identity=32.5757575757576, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 42383; Mature: 42252

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAAAPRAARAMSSAKRMREAAGAAPGDLPLDQILLGDSIQMMAGLPAGSVDLVFADPPY
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHCCCCCCEEEEECCCCC
NLQLGGELKRPDESLVDAVDDDWDRFDSFATYDAFTRAWLLAARRALKPNGTLFVIGSYH
CEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCH
NIFRVGAMMQDLGFWILNDIVWRKANPMPNFRGRRFTNAHETLIWAARSADAKYTFNYEA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHEEEEEECCCCCEEEECHHH
LKAGNEDVQVRSDWLFPLCTGQERLKDGAGKKLHPTQKPEALLARVLLSATKPGDVVLDP
HHCCCCCEEEECCCCCEECCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEC
FFGSGTTGAVAKRLRRSFIGIEREEAYAQAARARIDAVEPLPEDALVAPPSAREAPRVAF
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHH
SVLVERGLVTPGAELTDAKGRFRAVVRADGTLALAGGQNVGSIHRVGALAQGAEACNGWT
HHHHHHCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHCCCCE
FWHVEEQGRRHPIDTLRARLRAEMGIAAE
EEEECCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PAAAPRAARAMSSAKRMREAAGAAPGDLPLDQILLGDSIQMMAGLPAGSVDLVFADPPY
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHCCCCCCEEEEECCCCC
NLQLGGELKRPDESLVDAVDDDWDRFDSFATYDAFTRAWLLAARRALKPNGTLFVIGSYH
CEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCH
NIFRVGAMMQDLGFWILNDIVWRKANPMPNFRGRRFTNAHETLIWAARSADAKYTFNYEA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHEEEEEECCCCCEEEECHHH
LKAGNEDVQVRSDWLFPLCTGQERLKDGAGKKLHPTQKPEALLARVLLSATKPGDVVLDP
HHCCCCCEEEECCCCCEECCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEC
FFGSGTTGAVAKRLRRSFIGIEREEAYAQAARARIDAVEPLPEDALVAPPSAREAPRVAF
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHH
SVLVERGLVTPGAELTDAKGRFRAVVRADGTLALAGGQNVGSIHRVGALAQGAEACNGWT
HHHHHHCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHCCCCE
FWHVEEQGRRHPIDTLRARLRAEMGIAAE
EEEECCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9294447; 11481430 [H]