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The map label for this gene is lepA

Identifier: 154245110

GI number: 154245110

Start: 1337057

End: 1338862

Strand: Direct

Name: lepA

Synonym: Xaut_1162

Alternate gene names: 154245110

Gene position: 1337057-1338862 (Clockwise)

Preceding gene: 154245109

Following gene: 154245111

Centisome position: 25.19

GC content: 64.51

Gene sequence:

>1806_bases
ATGACCGCCAGCGCCCAGAAGAACATTCGCAACTTCTCCATCGTCGCCCACATCGATCATGGGAAATCGACCCTCGCCGA
CCGCCTGATCCAGATCACCGGCGGTCTCACCGAGCGGGAGATGACGGATCAGGTGCTCGACAGCATGGATATCGAGCGCG
AGCGCGGCATCACCATCAAGGCCCAGACGGTGCGCCTGTCCTACAAGGCCAAGGACGGCGAGACTTACATCCTCAACCTC
ATCGACACCCCCGGGCACGTGGACTTCGCCTACGAGGTGAACCGGTCGCTCGCCGCCGTGGAGGGTTCGCTGCTGGTGGT
GGATGCCTCCCAGGGCGTGGAGGCGCAGACGCTGGCCAACGTCTACCAGGCCATCGACAACAACCACGACATCGTCCCTG
TCCTGAACAAGGTGGACCTGCCTGCCGCCGAGCCGGAGAAGGTGAAGGCGCAGATCGAGGACGTGATCGGCCTCGACGCC
TCCGATGCCATCGGCATTTCCGCCAAGACCGGCCTCAACGTGGACCAGGTGCTGGAAGCCATCGTCACCCGCCTGCCGCC
GCCCATGGGCGACCGCGCCGCGCCGCTGAAGGCGCTGCTGGTGGATTCTTGGTACGACACCTACCTCGGCGTGGTGGTGC
TGGTGCGCATCATCGACGGCGTGCTGAAGAAGGGCCAGCGCATCAAGATGATGGGCTCGGGCGCTGTGAACGACGTGGAT
CGCGTGGGCGTGTTCACCCCGAAGATGGTGGCCATGGCCGAGCTTGGCCCCGGCGAGATCGGCTTCCTCACCGGCTCCAT
CAAGGAAGTGGCCGACACCCGCGTCGGCGACACCATCACCGAAGACAAGCGCCCCTGCGCCGACATGCTGCCGGGCTTCA
AGCCGGCGCAGCCGGTGGTGTTCTGCGGCCTGTTCCCGGTGGATGCGGCGCAGTTCGAGGATCTGCGCGCCGCCATGGGC
AAGCTGCGCCTCAACGACGCCAGCTTCTCCTACGAGATGGAAACCTCCGCCGCGCTGGGCTTCGGCTTCCGCTGCGGCTT
CCTCGGGTTGCTGCACCTGGAGATCATCCAGGAGCGGCTGGAGCGCGAGTTCAACCTCGACCTCATAGCCACCGCGCCCT
CGGTCATCTACGAGATCCAGATGACCGATGGTTCGGTCATCGACCTGCACAATCCGGCGGACATGCCGGACGTGGTGAAG
ATCGAGGAGATCCGCGAGCCGTGGATTCGCGCCACCATCCTCACGCCGGACGAGTATCTCGGCTCGGTGCTGAAGCTGTG
CCAGGACCGGCGCGGCACCCAGATCGAGCTCACCTATGTGGGCGCGCGGGCCATGGTGACCTATGACCTGCCCCTCAACG
AGGTGGTGTTCGATTTCTACGACCGGCTCAAATCCATCTCCAAGGGCTACGCCTCCTTCGACTACCAGATCACCGAGTAC
CGCGCCGGCGACCTGGTGAAGATGTCCATCCTGGTGAATTCGGAGCCGGTGGACGCCCTCTCCATGCTGGTGCACCGCGC
GCGCGCCGACGCGCGCGGCCGGGTGATGTGCGAGAAGCTGAAGGACCTGATCCCCCAGCATCTGTTCAACATCCCGATCC
AGGCCGCCATCGGCGCCAAGATCATTGCCCGCGAGACCATCAAGGCCCTGCGCAAGGACGTGACCGCCAAGTGCTACGGC
GGCGACATCTCCCGCAAGCGCAAGCTTCTGGACAAGCAGAAGGAAGGCAAGAAGAAGATGCGCCAGTTCGGCAAGGTGGA
GATCCCGCAGGAGGCCTTCATCGCCGCGCTGAAGATGGACGAATAG

Upstream 100 bases:

>100_bases
GGGCGCAGGCCGTCCCGGCGGGTCTCGCGCTATCCCTTCTCCTGCCCCGCGCCGGCCCTGCCGCCTTGTGGCGAACCCGC
GCCGATGCTATGCGGCGCGG

Downstream 100 bases:

>100_bases
CGCGCGCGCACTGGTCCTGTGTTGGCCTGAACGCGGTCCCGCCGCGCCGCCGCCGGCCCGTCTCTTGCATGATATTCAGG
CACAAGCCGAATCGGACCTG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 601; Mature: 600

Protein sequence:

>601_residues
MTASAQKNIRNFSIVAHIDHGKSTLADRLIQITGGLTEREMTDQVLDSMDIERERGITIKAQTVRLSYKAKDGETYILNL
IDTPGHVDFAYEVNRSLAAVEGSLLVVDASQGVEAQTLANVYQAIDNNHDIVPVLNKVDLPAAEPEKVKAQIEDVIGLDA
SDAIGISAKTGLNVDQVLEAIVTRLPPPMGDRAAPLKALLVDSWYDTYLGVVVLVRIIDGVLKKGQRIKMMGSGAVNDVD
RVGVFTPKMVAMAELGPGEIGFLTGSIKEVADTRVGDTITEDKRPCADMLPGFKPAQPVVFCGLFPVDAAQFEDLRAAMG
KLRLNDASFSYEMETSAALGFGFRCGFLGLLHLEIIQERLEREFNLDLIATAPSVIYEIQMTDGSVIDLHNPADMPDVVK
IEEIREPWIRATILTPDEYLGSVLKLCQDRRGTQIELTYVGARAMVTYDLPLNEVVFDFYDRLKSISKGYASFDYQITEY
RAGDLVKMSILVNSEPVDALSMLVHRARADARGRVMCEKLKDLIPQHLFNIPIQAAIGAKIIARETIKALRKDVTAKCYG
GDISRKRKLLDKQKEGKKKMRQFGKVEIPQEAFIAALKMDE

Sequences:

>Translated_601_residues
MTASAQKNIRNFSIVAHIDHGKSTLADRLIQITGGLTEREMTDQVLDSMDIERERGITIKAQTVRLSYKAKDGETYILNL
IDTPGHVDFAYEVNRSLAAVEGSLLVVDASQGVEAQTLANVYQAIDNNHDIVPVLNKVDLPAAEPEKVKAQIEDVIGLDA
SDAIGISAKTGLNVDQVLEAIVTRLPPPMGDRAAPLKALLVDSWYDTYLGVVVLVRIIDGVLKKGQRIKMMGSGAVNDVD
RVGVFTPKMVAMAELGPGEIGFLTGSIKEVADTRVGDTITEDKRPCADMLPGFKPAQPVVFCGLFPVDAAQFEDLRAAMG
KLRLNDASFSYEMETSAALGFGFRCGFLGLLHLEIIQERLEREFNLDLIATAPSVIYEIQMTDGSVIDLHNPADMPDVVK
IEEIREPWIRATILTPDEYLGSVLKLCQDRRGTQIELTYVGARAMVTYDLPLNEVVFDFYDRLKSISKGYASFDYQITEY
RAGDLVKMSILVNSEPVDALSMLVHRARADARGRVMCEKLKDLIPQHLFNIPIQAAIGAKIIARETIKALRKDVTAKCYG
GDISRKRKLLDKQKEGKKKMRQFGKVEIPQEAFIAALKMDE
>Mature_600_residues
TASAQKNIRNFSIVAHIDHGKSTLADRLIQITGGLTEREMTDQVLDSMDIERERGITIKAQTVRLSYKAKDGETYILNLI
DTPGHVDFAYEVNRSLAAVEGSLLVVDASQGVEAQTLANVYQAIDNNHDIVPVLNKVDLPAAEPEKVKAQIEDVIGLDAS
DAIGISAKTGLNVDQVLEAIVTRLPPPMGDRAAPLKALLVDSWYDTYLGVVVLVRIIDGVLKKGQRIKMMGSGAVNDVDR
VGVFTPKMVAMAELGPGEIGFLTGSIKEVADTRVGDTITEDKRPCADMLPGFKPAQPVVFCGLFPVDAAQFEDLRAAMGK
LRLNDASFSYEMETSAALGFGFRCGFLGLLHLEIIQERLEREFNLDLIATAPSVIYEIQMTDGSVIDLHNPADMPDVVKI
EEIREPWIRATILTPDEYLGSVLKLCQDRRGTQIELTYVGARAMVTYDLPLNEVVFDFYDRLKSISKGYASFDYQITEYR
AGDLVKMSILVNSEPVDALSMLVHRARADARGRVMCEKLKDLIPQHLFNIPIQAAIGAKIIARETIKALRKDVTAKCYGG
DISRKRKLLDKQKEGKKKMRQFGKVEIPQEAFIAALKMDE

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=601, Percent_Identity=49.9168053244592, Blast_Score=625, Evalue=1e-179,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=45.8646616541353, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI4503483, Length=146, Percent_Identity=41.0958904109589, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI25306283, Length=134, Percent_Identity=47.0149253731343, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI19923640, Length=134, Percent_Identity=47.0149253731343, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI25306287, Length=134, Percent_Identity=47.0149253731343, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI18390331, Length=162, Percent_Identity=35.1851851851852, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=42.3423423423423, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=42.3423423423423, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=42.3423423423423, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI217272894, Length=134, Percent_Identity=35.8208955223881, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI217272892, Length=134, Percent_Identity=35.8208955223881, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI53729339, Length=226, Percent_Identity=30.0884955752212, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI53729337, Length=226, Percent_Identity=30.0884955752212, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI34147630, Length=244, Percent_Identity=29.5081967213115, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1788922, Length=593, Percent_Identity=60.2023608768971, Blast_Score=725, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=502, Percent_Identity=27.8884462151394, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=38.7096774193548, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1790835, Length=154, Percent_Identity=32.4675324675325, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1789559, Length=228, Percent_Identity=28.0701754385965, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=39.672131147541, Blast_Score=469, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17556745, Length=155, Percent_Identity=38.0645161290323, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=38.0597014925373, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=38.5185185185185, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=158, Percent_Identity=36.0759493670886, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=145, Percent_Identity=37.9310344827586, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=33.8345864661654, Blast_Score=84, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=601, Percent_Identity=46.7554076539101, Blast_Score=550, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=40.9722222222222, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=40.9722222222222, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6323098, Length=159, Percent_Identity=38.9937106918239, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=112, Percent_Identity=39.2857142857143, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=170, Percent_Identity=33.5294117647059, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=248, Percent_Identity=28.2258064516129, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI78706572, Length=601, Percent_Identity=44.0931780366057, Blast_Score=539, Evalue=1e-153,
Organism=Drosophila melanogaster, GI24582462, Length=183, Percent_Identity=35.5191256830601, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585711, Length=150, Percent_Identity=36, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585713, Length=150, Percent_Identity=36, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585709, Length=150, Percent_Identity=36, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=40.2877697841727, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI221458488, Length=154, Percent_Identity=40.2597402597403, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357743, Length=162, Percent_Identity=34.5679012345679, Blast_Score=90, Evalue=6e-18,
Organism=Drosophila melanogaster, GI28572034, Length=261, Percent_Identity=29.8850574712644, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_XANP2 (A7IEG8)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001416068.1
- ProteinModelPortal:   A7IEG8
- SMR:   A7IEG8
- STRING:   A7IEG8
- GeneID:   5421840
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_1162
- eggNOG:   COG0481
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- BioCyc:   XAUT78245:XAUT_1162-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 66330; Mature: 66198

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASAQKNIRNFSIVAHIDHGKSTLADRLIQITGGLTEREMTDQVLDSMDIERERGITIK
CCCCCHHCCCEEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEE
AQTVRLSYKAKDGETYILNLIDTPGHVDFAYEVNRSLAAVEGSLLVVDASQGVEAQTLAN
EEEEEEEEEECCCCEEEEEEECCCCCEEEEEECCCHHEEECCCEEEEECCCCCCHHHHHH
VYQAIDNNHDIVPVLNKVDLPAAEPEKVKAQIEDVIGLDASDAIGISAKTGLNVDQVLEA
HHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
IVTRLPPPMGDRAAPLKALLVDSWYDTYLGVVVLVRIIDGVLKKGQRIKMMGSGAVNDVD
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHH
RVGVFTPKMVAMAELGPGEIGFLTGSIKEVADTRVGDTITEDKRPCADMLPGFKPAQPVV
HCCCCCCHHHHHCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCEE
FCGLFPVDAAQFEDLRAAMGKLRLNDASFSYEMETSAALGFGFRCGFLGLLHLEIIQERL
EEECCCCCHHHHHHHHHHHHHEEECCCCCEEEECCCHHHCCCHHHHHHHHHHHHHHHHHH
EREFNLDLIATAPSVIYEIQMTDGSVIDLHNPADMPDVVKIEEIREPWIRATILTPDEYL
HHHCCCEEEEECCEEEEEEEECCCCEEECCCCCCCCCCEEHHHHCCCHHEEEEECCHHHH
GSVLKLCQDRRGTQIELTYVGARAMVTYDLPLNEVVFDFYDRLKSISKGYASFDYQITEY
HHHHHHHHHCCCCEEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
RAGDLVKMSILVNSEPVDALSMLVHRARADARGRVMCEKLKDLIPQHLFNIPIQAAIGAK
CCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCHH
IIARETIKALRKDVTAKCYGGDISRKRKLLDKQKEGKKKMRQFGKVEIPQEAFIAALKMD
HHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
E
C
>Mature Secondary Structure 
TASAQKNIRNFSIVAHIDHGKSTLADRLIQITGGLTEREMTDQVLDSMDIERERGITIK
CCCCHHCCCEEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEE
AQTVRLSYKAKDGETYILNLIDTPGHVDFAYEVNRSLAAVEGSLLVVDASQGVEAQTLAN
EEEEEEEEEECCCCEEEEEEECCCCCEEEEEECCCHHEEECCCEEEEECCCCCCHHHHHH
VYQAIDNNHDIVPVLNKVDLPAAEPEKVKAQIEDVIGLDASDAIGISAKTGLNVDQVLEA
HHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
IVTRLPPPMGDRAAPLKALLVDSWYDTYLGVVVLVRIIDGVLKKGQRIKMMGSGAVNDVD
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHH
RVGVFTPKMVAMAELGPGEIGFLTGSIKEVADTRVGDTITEDKRPCADMLPGFKPAQPVV
HCCCCCCHHHHHCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCEE
FCGLFPVDAAQFEDLRAAMGKLRLNDASFSYEMETSAALGFGFRCGFLGLLHLEIIQERL
EEECCCCCHHHHHHHHHHHHHEEECCCCCEEEECCCHHHCCCHHHHHHHHHHHHHHHHHH
EREFNLDLIATAPSVIYEIQMTDGSVIDLHNPADMPDVVKIEEIREPWIRATILTPDEYL
HHHCCCEEEEECCEEEEEEEECCCCEEECCCCCCCCCCEEHHHHCCCHHEEEEECCHHHH
GSVLKLCQDRRGTQIELTYVGARAMVTYDLPLNEVVFDFYDRLKSISKGYASFDYQITEY
HHHHHHHHHCCCCEEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
RAGDLVKMSILVNSEPVDALSMLVHRARADARGRVMCEKLKDLIPQHLFNIPIQAAIGAK
CCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCHH
IIARETIKALRKDVTAKCYGGDISRKRKLLDKQKEGKKKMRQFGKVEIPQEAFIAALKMD
HHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA