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The map label for this gene is pcaK [H]

Identifier: 154245085

GI number: 154245085

Start: 1308378

End: 1309751

Strand: Direct

Name: pcaK [H]

Synonym: Xaut_1137

Alternate gene names: 154245085

Gene position: 1308378-1309751 (Clockwise)

Preceding gene: 154245084

Following gene: 154245086

Centisome position: 24.64

GC content: 68.78

Gene sequence:

>1374_bases
ATGTCTGCCCCAGCTCAGGCGGGCCCAAGGCCCGTGAACATCCAGCAGCTTATCGACGAGAGCCCGGTGTCGCGGTTCCA
GCTGCTTATCATCGTGCTGTGCTTTCTCATCGTCGCGGTGGACGGCTTCGACACGGCGGCCGCGGCCTTCATCGCGCCGG
CCATCCGCGCCGAATGGCAGCTCACCCCGGCCGTGCTCGGTCCCATGTTCGGCGCCGGCCTGTTCGGGCTGATGGTGGGG
GCGCTGGTCATCGGCCCGTTTGCCGACCGCTTCGGGCGCAAGCCGCTCCTGATCTTCTCGGTGGTCTTCTTCGGCCTCGC
CTCACTCGGGGCGGCCTTCTCCCATTCCATCACCGAACTGACCTGGTGGCGGTTCGTCACCGGACTCGGGCTCGGCGCCG
CCATGCCCAGCGCCATCACCCTCACCTCGGAATATTGCCCCGAGCGCAGGCGCTCCTTCCTCGTCACCATCATGTTCTGC
GGCTTCACTTTGGGCTCGGCCATGGGCGGCCTGCTGGCGGCGCAGCTGGTGGCGGAACACGGCTGGCGCTCGGTGCTGGT
GGTGGGCGGCGCGGTGCCGCTGGTGGTGGCTGTTCTGCTGTGGGCGCTCTTGCCCGAATCCATCCGCTTCCTGGTGCTGA
AGGATCCCCGCTCGGCCGCCGCCGCGCGCAACCTTGCCCGCATCGCCCCGAAGGCGGACCTCGCCGGCGCGGTCTTCGTC
GGGCCGAAGAAGGCCAAGGGGCTGCCGGTGCAGCAATTGTTCTCGCGTAATCTCGTGGGCGGAACGCTGTTGCTGTGGCT
CGCCTTCTTCATGAGCCTGCTGGTCTATTATCTCCTCACCAACTGGCTGCCCACCTTCATCAATGCCGGCGGCGCCTCGA
TCCAGCAGGCGGCGCTGGTCACCATGATGCTGCAGCTCGGCGGCACCCTCGGCGGCGTGGTGCTGGGCCTGTTCATGGAC
AAGGTGAACCCCCACGCCCTGCTCGCCTCCGCCTATGTGGGCGGCGGACTGTGCGTCACCGCCATCGGCAATTCCACCGG
AGTACCCGGCCTGCTGCCGGTGGCGGTGTTCGGCGCCGGCTTCTGCATCGCCGGCTCGCAGATCGGCATCAATGCGCTGG
CGGCGGCCTATTATCCCACCGCGAGCCGGGCCACCGGCGTCAGCTGGGCCAACGGCATCGGCCGCTCGGGCTCGGTGGTG
GGCTCCATGGTGGGCGGGCTGCTGCTCTCCTTCCACTGGGGCTTCGAGACCATCTTCGCCATCGCCGCCGCGCCCGCGCT
GGTGGCGGGCCTCGCCATTTTCCTGAAGGGCCGGCTCCAGTCCGGGGCGACGCTGGATGCAGCTCCGGTGGCGCAGGTCG
CGCAGGAAGGCTGA

Upstream 100 bases:

>100_bases
CTGCGAAAGGCCCGCCCGCCCCGCAACCCAAGGCCATCGCGGCCCAAGACCAGCACGGCAAAGCCGGCACACCCTTCAAT
TTCAGAGAGGAAAAACGCTC

Downstream 100 bases:

>100_bases
TCCGAACGACGGTCAATCGGGCGGCTCCTCTCTCACGGGCGGGGCCGCCCGAGGCCGTTCCGGGAGCGCGCGCGGCCGGC
GCATGTGGGATGATCCGACC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSAPAQAGPRPVNIQQLIDESPVSRFQLLIIVLCFLIVAVDGFDTAAAAFIAPAIRAEWQLTPAVLGPMFGAGLFGLMVG
ALVIGPFADRFGRKPLLIFSVVFFGLASLGAAFSHSITELTWWRFVTGLGLGAAMPSAITLTSEYCPERRRSFLVTIMFC
GFTLGSAMGGLLAAQLVAEHGWRSVLVVGGAVPLVVAVLLWALLPESIRFLVLKDPRSAAAARNLARIAPKADLAGAVFV
GPKKAKGLPVQQLFSRNLVGGTLLLWLAFFMSLLVYYLLTNWLPTFINAGGASIQQAALVTMMLQLGGTLGGVVLGLFMD
KVNPHALLASAYVGGGLCVTAIGNSTGVPGLLPVAVFGAGFCIAGSQIGINALAAAYYPTASRATGVSWANGIGRSGSVV
GSMVGGLLLSFHWGFETIFAIAAAPALVAGLAIFLKGRLQSGATLDAAPVAQVAQEG

Sequences:

>Translated_457_residues
MSAPAQAGPRPVNIQQLIDESPVSRFQLLIIVLCFLIVAVDGFDTAAAAFIAPAIRAEWQLTPAVLGPMFGAGLFGLMVG
ALVIGPFADRFGRKPLLIFSVVFFGLASLGAAFSHSITELTWWRFVTGLGLGAAMPSAITLTSEYCPERRRSFLVTIMFC
GFTLGSAMGGLLAAQLVAEHGWRSVLVVGGAVPLVVAVLLWALLPESIRFLVLKDPRSAAAARNLARIAPKADLAGAVFV
GPKKAKGLPVQQLFSRNLVGGTLLLWLAFFMSLLVYYLLTNWLPTFINAGGASIQQAALVTMMLQLGGTLGGVVLGLFMD
KVNPHALLASAYVGGGLCVTAIGNSTGVPGLLPVAVFGAGFCIAGSQIGINALAAAYYPTASRATGVSWANGIGRSGSVV
GSMVGGLLLSFHWGFETIFAIAAAPALVAGLAIFLKGRLQSGATLDAAPVAQVAQEG
>Mature_456_residues
SAPAQAGPRPVNIQQLIDESPVSRFQLLIIVLCFLIVAVDGFDTAAAAFIAPAIRAEWQLTPAVLGPMFGAGLFGLMVGA
LVIGPFADRFGRKPLLIFSVVFFGLASLGAAFSHSITELTWWRFVTGLGLGAAMPSAITLTSEYCPERRRSFLVTIMFCG
FTLGSAMGGLLAAQLVAEHGWRSVLVVGGAVPLVVAVLLWALLPESIRFLVLKDPRSAAAARNLARIAPKADLAGAVFVG
PKKAKGLPVQQLFSRNLVGGTLLLWLAFFMSLLVYYLLTNWLPTFINAGGASIQQAALVTMMLQLGGTLGGVVLGLFMDK
VNPHALLASAYVGGGLCVTAIGNSTGVPGLLPVAVFGAGFCIAGSQIGINALAAAYYPTASRATGVSWANGIGRSGSVVG
SMVGGLLLSFHWGFETIFAIAAAPALVAGLAIFLKGRLQSGATLDAAPVAQVAQEG

Specific function: Transporter for 4-hydroxybenzoate. Also required for chemotaxis to aromatic acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=409, Percent_Identity=25.6723716381418, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI213021148, Length=437, Percent_Identity=21.5102974828375, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI8923870, Length=395, Percent_Identity=26.3291139240506, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI296080734, Length=180, Percent_Identity=32.2222222222222, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI296080721, Length=180, Percent_Identity=32.2222222222222, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI296080719, Length=173, Percent_Identity=33.5260115606936, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI24497499, Length=173, Percent_Identity=33.5260115606936, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI7662270, Length=212, Percent_Identity=27.8301886792453, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI170650643, Length=200, Percent_Identity=29.5, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI87081723, Length=417, Percent_Identity=37.8896882494005, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI87082159, Length=390, Percent_Identity=26.1538461538462, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788074, Length=352, Percent_Identity=25.5681818181818, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788068, Length=469, Percent_Identity=23.8805970149254, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI87082231, Length=168, Percent_Identity=25.5952380952381, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789515, Length=378, Percent_Identity=24.0740740740741, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786229, Length=162, Percent_Identity=29.6296296296296, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789207, Length=341, Percent_Identity=26.099706744868, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI87082404, Length=371, Percent_Identity=20.7547169811321, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=276, Percent_Identity=25.3623188405797, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71980582, Length=340, Percent_Identity=23.5294117647059, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71980584, Length=340, Percent_Identity=23.8235294117647, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24640198, Length=479, Percent_Identity=22.1294363256785, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24640196, Length=479, Percent_Identity=22.1294363256785, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24640200, Length=479, Percent_Identity=22.1294363256785, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19922874, Length=437, Percent_Identity=23.3409610983982, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI62484254, Length=365, Percent_Identity=24.6575342465753, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47299; Mature: 47167

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPAQAGPRPVNIQQLIDESPVSRFQLLIIVLCFLIVAVDGFDTAAAAFIAPAIRAEWQ
CCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
LTPAVLGPMFGAGLFGLMVGALVIGPFADRFGRKPLLIFSVVFFGLASLGAAFSHSITEL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TWWRFVTGLGLGAAMPSAITLTSEYCPERRRSFLVTIMFCGFTLGSAMGGLLAAQLVAEH
HHHHHHHHCCHHHCCCCCEEECHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GWRSVLVVGGAVPLVVAVLLWALLPESIRFLVLKDPRSAAAARNLARIAPKADLAGAVFV
CCCEEEEECCHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHCCCCCCCCEEEE
GPKKAKGLPVQQLFSRNLVGGTLLLWLAFFMSLLVYYLLTNWLPTFINAGGASIQQAALV
CCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
TMMLQLGGTLGGVVLGLFMDKVNPHALLASAYVGGGLCVTAIGNSTGVPGLLPVAVFGAG
HHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCC
FCIAGSQIGINALAAAYYPTASRATGVSWANGIGRSGSVVGSMVGGLLLSFHWGFETIFA
HHCCCCCCCHHHHHHHHCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IAAAPALVAGLAIFLKGRLQSGATLDAAPVAQVAQEG
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
SAPAQAGPRPVNIQQLIDESPVSRFQLLIIVLCFLIVAVDGFDTAAAAFIAPAIRAEWQ
CCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
LTPAVLGPMFGAGLFGLMVGALVIGPFADRFGRKPLLIFSVVFFGLASLGAAFSHSITEL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TWWRFVTGLGLGAAMPSAITLTSEYCPERRRSFLVTIMFCGFTLGSAMGGLLAAQLVAEH
HHHHHHHHCCHHHCCCCCEEECHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GWRSVLVVGGAVPLVVAVLLWALLPESIRFLVLKDPRSAAAARNLARIAPKADLAGAVFV
CCCEEEEECCHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHCCCCCCCCEEEE
GPKKAKGLPVQQLFSRNLVGGTLLLWLAFFMSLLVYYLLTNWLPTFINAGGASIQQAALV
CCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
TMMLQLGGTLGGVVLGLFMDKVNPHALLASAYVGGGLCVTAIGNSTGVPGLLPVAVFGAG
HHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCC
FCIAGSQIGINALAAAYYPTASRATGVSWANGIGRSGSVVGSMVGGLLLSFHWGFETIFA
HHCCCCCCCHHHHHHHHCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IAAAPALVAGLAIFLKGRLQSGATLDAAPVAQVAQEG
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961399 [H]