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The map label for this gene is hsdM [H]

Identifier: 154245044

GI number: 154245044

Start: 1264446

End: 1265906

Strand: Reverse

Name: hsdM [H]

Synonym: Xaut_1096

Alternate gene names: 154245044

Gene position: 1265906-1264446 (Counterclockwise)

Preceding gene: 154245045

Following gene: 154245043

Centisome position: 23.84

GC content: 65.64

Gene sequence:

>1461_bases
ATGACCTCCTCCACCACCGACATCGTGGCCAAGCTCTGGAGCCTGTGCCATGTGCTCCGCGACGACGGCGTCACCTACAA
CGAGTATGTGACCGAGCTGACCTTCCTCCTATTCCTCAAGATGCTGGAGGAGACCGGCCACGAGGAGCGGTTGCCGGAGG
GCTGGCGCTGGGGCCTCCTCGCGAAGAAGGAGGGCATGGACCAGCTCGACTATTACAAGGCCATGCTGCTCGCCCTCGGC
AAGGCGCCCGACAAGCTGGTCGGCGCCATCTTCACCGATGCGCAGACCAAGCTGCGCAAGCCCACGAACCTCAAGGCGCT
CACCTCCAGCATCGACCTGCTGGATTGGTTCTCCGCCCGAGAGGAGGGGCTCGGCACCCTCTACGAAGGCCTGCTGGAGA
AGAACGCGGCCGACAAGAAATCCGGTGCCGGGCAATATTTCACGCCCCGCCCCCTCATCGATTGCATCGTGCGGCTGATG
AAGCCGCAGCCGGGCGAGATCGTGCAGGACCCGGCAGCGGGCACGGCGGGCTTCCTTGTGGCGGCCGATCGCTACATCAA
GGACCGCACCGACGACCTCTTCGAGCTGACAGAGGCGCAAGGCTTTTTCCAGCGCAACAATGCCTTCGTCGGCGCCGAAC
TGGTGCCGGACACCCACCGCCTCTGCCTCATGAACCTGCTTCTGCATGGCATCGAGGGCGGAGTGGAGAGCATGGACACC
CTCTCCCCCGATGGCGAGGGGCTGCCGAAGGCGCACCTCATCCTCACCAATCCGCCCTTCGGCACCAAGAAGGGCGGCGG
GCGGCCCACGCGCACCGACTTTTCCGTAACGGCGGACACATCCAACAAGCAGCTCGCCTTCGTGGAACACATCGTGCGAA
GCCTTCGCCCCGGCGGACGCGCCGCCGTGGTGGTGCCGGACAATGTGCTGTTCGAGGACAACACCGGCCGGCGCCTGCGC
TCATGGCTCATGGAGCTGTGCGATCTGCACACCATCCTGCGTCTGCCCACCGGCATCTTCTATGCGCAGGGCGTGAAGAC
CAACGTGCTGTTCTTCCGGCGCGGTACAAATGACAAGGCCAACATCAAGGCCGTATGGGTTTACGACATGCGGGCCAACA
TGCCCGCCTTCGGCAAGACGCGACCCCTCACCATCGCCGATTTCGCCGCCTTCGAGGCCGCCTATGGGGATGACCCAAAC
GGCGGGGCGACGCGGACGGACGAGGGGGAGGAGGGCCGCTGGCGCTGCTTCTCCCGCGCCGCCATCGCCGCACGCAACGA
CAATCTCGACATCTCCTGGCTGCGCGACACCGAGGCGGAGGCCGAGGAAACCCTGACCGAGCCGGAAGACATCGCCGCCG
CCCTCATCGGGCACCTGAAGGCGGCGCTGGAGGAGATCGAGGCTCTGGCGGAAGAGCTGGAGCCGGAGGATGAAGCGGCG
GTGCCGGAGGCGGCGGAATGA

Upstream 100 bases:

>100_bases
GTCATATGAAGGATTTTGACATCGAGGACGAAGATGCCGCGGCGTACATCGACACAGCGCTCTCCTTGGCTGCCCAACTG
CATCGCCTGAAAGAAAAACA

Downstream 100 bases:

>100_bases
GTGAAGCGCGGTGGCAAGTTCCCCATTCGTGGCTCTGGGCATCGTTTGGTGAAGTTGCAGACATCGTCGGCGGGGGAACG
CCGCCTACCGGAGATGAGGC

Product: N-6 DNA methylase

Products: NA

Alternate protein names: M.EcoKI [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTSSTTDIVAKLWSLCHVLRDDGVTYNEYVTELTFLLFLKMLEETGHEERLPEGWRWGLLAKKEGMDQLDYYKAMLLALG
KAPDKLVGAIFTDAQTKLRKPTNLKALTSSIDLLDWFSAREEGLGTLYEGLLEKNAADKKSGAGQYFTPRPLIDCIVRLM
KPQPGEIVQDPAAGTAGFLVAADRYIKDRTDDLFELTEAQGFFQRNNAFVGAELVPDTHRLCLMNLLLHGIEGGVESMDT
LSPDGEGLPKAHLILTNPPFGTKKGGGRPTRTDFSVTADTSNKQLAFVEHIVRSLRPGGRAAVVVPDNVLFEDNTGRRLR
SWLMELCDLHTILRLPTGIFYAQGVKTNVLFFRRGTNDKANIKAVWVYDMRANMPAFGKTRPLTIADFAAFEAAYGDDPN
GGATRTDEGEEGRWRCFSRAAIAARNDNLDISWLRDTEAEAEETLTEPEDIAAALIGHLKAALEEIEALAEELEPEDEAA
VPEAAE

Sequences:

>Translated_486_residues
MTSSTTDIVAKLWSLCHVLRDDGVTYNEYVTELTFLLFLKMLEETGHEERLPEGWRWGLLAKKEGMDQLDYYKAMLLALG
KAPDKLVGAIFTDAQTKLRKPTNLKALTSSIDLLDWFSAREEGLGTLYEGLLEKNAADKKSGAGQYFTPRPLIDCIVRLM
KPQPGEIVQDPAAGTAGFLVAADRYIKDRTDDLFELTEAQGFFQRNNAFVGAELVPDTHRLCLMNLLLHGIEGGVESMDT
LSPDGEGLPKAHLILTNPPFGTKKGGGRPTRTDFSVTADTSNKQLAFVEHIVRSLRPGGRAAVVVPDNVLFEDNTGRRLR
SWLMELCDLHTILRLPTGIFYAQGVKTNVLFFRRGTNDKANIKAVWVYDMRANMPAFGKTRPLTIADFAAFEAAYGDDPN
GGATRTDEGEEGRWRCFSRAAIAARNDNLDISWLRDTEAEAEETLTEPEDIAAALIGHLKAALEEIEALAEELEPEDEAA
VPEAAE
>Mature_485_residues
TSSTTDIVAKLWSLCHVLRDDGVTYNEYVTELTFLLFLKMLEETGHEERLPEGWRWGLLAKKEGMDQLDYYKAMLLALGK
APDKLVGAIFTDAQTKLRKPTNLKALTSSIDLLDWFSAREEGLGTLYEGLLEKNAADKKSGAGQYFTPRPLIDCIVRLMK
PQPGEIVQDPAAGTAGFLVAADRYIKDRTDDLFELTEAQGFFQRNNAFVGAELVPDTHRLCLMNLLLHGIEGGVESMDTL
SPDGEGLPKAHLILTNPPFGTKKGGGRPTRTDFSVTADTSNKQLAFVEHIVRSLRPGGRAAVVVPDNVLFEDNTGRRLRS
WLMELCDLHTILRLPTGIFYAQGVKTNVLFFRRGTNDKANIKAVWVYDMRANMPAFGKTRPLTIADFAAFEAAYGDDPNG
GATRTDEGEEGRWRCFSRAAIAARNDNLDISWLRDTEAEAEETLTEPEDIAAALIGHLKAALEEIEALAEELEPEDEAAV
PEAAE

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=517, Percent_Identity=51.4506769825919, Blast_Score=494, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 53739; Mature: 53608

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSTTDIVAKLWSLCHVLRDDGVTYNEYVTELTFLLFLKMLEETGHEERLPEGWRWGLL
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCE
AKKEGMDQLDYYKAMLLALGKAPDKLVGAIFTDAQTKLRKPTNLKALTSSIDLLDWFSAR
ECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
EEGLGTLYEGLLEKNAADKKSGAGQYFTPRPLIDCIVRLMKPQPGEIVQDPAAGTAGFLV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHCCCCCCCCHHHH
AADRYIKDRTDDLFELTEAQGFFQRNNAFVGAELVPDTHRLCLMNLLLHGIEGGVESMDT
HHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHC
LSPDGEGLPKAHLILTNPPFGTKKGGGRPTRTDFSVTADTSNKQLAFVEHIVRSLRPGGR
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCC
AAVVVPDNVLFEDNTGRRLRSWLMELCDLHTILRLPTGIFYAQGVKTNVLFFRRGTNDKA
EEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCCC
NIKAVWVYDMRANMPAFGKTRPLTIADFAAFEAAYGDDPNGGATRTDEGEEGRWRCFSRA
CEEEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
AIAARNDNLDISWLRDTEAEAEETLTEPEDIAAALIGHLKAALEEIEALAEELEPEDEAA
HHEECCCCCEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VPEAAE
CCCCCC
>Mature Secondary Structure 
TSSTTDIVAKLWSLCHVLRDDGVTYNEYVTELTFLLFLKMLEETGHEERLPEGWRWGLL
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCE
AKKEGMDQLDYYKAMLLALGKAPDKLVGAIFTDAQTKLRKPTNLKALTSSIDLLDWFSAR
ECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
EEGLGTLYEGLLEKNAADKKSGAGQYFTPRPLIDCIVRLMKPQPGEIVQDPAAGTAGFLV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHCCCCCCCCHHHH
AADRYIKDRTDDLFELTEAQGFFQRNNAFVGAELVPDTHRLCLMNLLLHGIEGGVESMDT
HHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHC
LSPDGEGLPKAHLILTNPPFGTKKGGGRPTRTDFSVTADTSNKQLAFVEHIVRSLRPGGR
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCC
AAVVVPDNVLFEDNTGRRLRSWLMELCDLHTILRLPTGIFYAQGVKTNVLFFRRGTNDKA
EEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCCC
NIKAVWVYDMRANMPAFGKTRPLTIADFAAFEAAYGDDPNGGATRTDEGEEGRWRCFSRA
CEEEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
AIAARNDNLDISWLRDTEAEAEETLTEPEDIAAALIGHLKAALEEIEALAEELEPEDEAA
HHEECCCCCEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VPEAAE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3323532; 7610040; 9278503 [H]