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The map label for this gene is hsdS [H]

Identifier: 154245043

GI number: 154245043

Start: 1263097

End: 1264449

Strand: Reverse

Name: hsdS [H]

Synonym: Xaut_1095

Alternate gene names: 154245043

Gene position: 1264449-1263097 (Counterclockwise)

Preceding gene: 154245044

Following gene: 154245042

Centisome position: 23.82

GC content: 58.68

Gene sequence:

>1353_bases
ATGAGTGAAGCGCGGTGGCAAGTTCCCCATTCGTGGCTCTGGGCATCGTTTGGTGAAGTTGCAGACATCGTCGGCGGGGG
AACGCCGCCTACCGGAGATGAGGCCAACTTCACCAAGCAAGGAGTTCCGTGGCTCACCCCAGCAGACTTGACGGGATATC
GCGAGACCTACATCTCGCGCGGGAGGCGTGATCTTAGCGAAAAGGGTTATCGCGAATCAGCTGCTCGCCTACTACCTAAA
GGCACGGTGCTCTTTTCGTCGCGAGCGCCGGTCGGCTATTGCGCCATTGCATCGGAGAATGTCAGCACAAACCAGGGCTT
CAAAAGCTTTATTCTGAAAGGCGATATCTCCCCCGAATACGTCCGGCACTACCTTCTGGGATCGACCGAGTACGCAGAAA
GCAAAGCGAGCGGGACAACCTTCAAGGAGCTTTCAGGCAGCCGAGCCACAGAGCTTGCGCTGCCTCTCCCACCCCTGCCC
GAACAGCGGCGGATCGTGGCGAAGATCGACAGCCTGACGGCCAAGTCCAGACGCGCCCGCGACCACCTCGAGCACATCCC
CCGCCTCGTCGAGAAGTACAAACAGGCCATCCTCGCCGCCGCATTTGATGGAAGGCTCACCGAGCTTAGTCCACATGATA
TTGTTCATCCGGAGCTTGGCGAGCTGATCGAATTTGGCCCCCAGAATGGGTTGTACTTGCCGAAAGATCGATATGGTGAA
GGCACCCCAATATTACGCATCCAGAACTATGGATTTAATTTTATAGATGAGCCGACGAATTGGCATAGGGTGACGGTATC
TGACGCAATCGCCGCGCAGTTCGCCATGTCTGACGGAGATTTGATTATTAATCGCGTCAACAGCCCGTCGCATCTTGGAA
AATCCATGGTCGTGACCAAAGCGATGGCTGGCGCGATATTTGAATCAAACATGATGAGGATCAGGCTCAATGCACTTGCG
GAGCCGAAATTTGTGCAGCTTTACCTATCATCATCTCAGGGCCGAGGCAGCCTCACGAAAGACGCGAAATGGGCGGTGAA
CCAAGCGAGCATCAATCAGGGAGACGTTTCGCGAACCCCTGTGCCACTACCTGGGCTATCAGACCAAATTGCTGTTCTAG
ATCGCATCGAAACCGCTTTCGCCTGGATCGACCGCCTCGCCGCCGAGGCCACCAGCGCCCGCACCCTCATCGACCGGCTG
GATCAGGCCGTGCTCGCAAAAGCCTTCCGAGGCGAGCTGGTGCCGCAGGACCCCGCCGACGAGCCGGCGAGCGTGCTGCT
TGAGCGCATCCGGGCAGAGCGCGGCGCGGCTCCCAAGGCCCGGCGCGGGCGGCGGCCGGCGGCGGAGGCGTGA

Upstream 100 bases:

>100_bases
CCGCCCTCATCGGGCACCTGAAGGCGGCGCTGGAGGAGATCGAGGCTCTGGCGGAAGAGCTGGAGCCGGAGGATGAAGCG
GCGGTGCCGGAGGCGGCGGA

Downstream 100 bases:

>100_bases
GATGCCCATTCCCGATTATCAGACCCTCATGCTGCCTCTTCTGAAGATCGCTGCCGAGGGCGAGACGCGCATTCCCGAAC
TGGAAGAGCGCATCTGCAAC

Product: restriction modification system DNA specificity subunit

Products: NA

Alternate protein names: S.EcoKI; Type I restriction enzyme EcoKI specificity protein; S protein [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSEARWQVPHSWLWASFGEVADIVGGGTPPTGDEANFTKQGVPWLTPADLTGYRETYISRGRRDLSEKGYRESAARLLPK
GTVLFSSRAPVGYCAIASENVSTNQGFKSFILKGDISPEYVRHYLLGSTEYAESKASGTTFKELSGSRATELALPLPPLP
EQRRIVAKIDSLTAKSRRARDHLEHIPRLVEKYKQAILAAAFDGRLTELSPHDIVHPELGELIEFGPQNGLYLPKDRYGE
GTPILRIQNYGFNFIDEPTNWHRVTVSDAIAAQFAMSDGDLIINRVNSPSHLGKSMVVTKAMAGAIFESNMMRIRLNALA
EPKFVQLYLSSSQGRGSLTKDAKWAVNQASINQGDVSRTPVPLPGLSDQIAVLDRIETAFAWIDRLAAEATSARTLIDRL
DQAVLAKAFRGELVPQDPADEPASVLLERIRAERGAAPKARRGRRPAAEA

Sequences:

>Translated_450_residues
MSEARWQVPHSWLWASFGEVADIVGGGTPPTGDEANFTKQGVPWLTPADLTGYRETYISRGRRDLSEKGYRESAARLLPK
GTVLFSSRAPVGYCAIASENVSTNQGFKSFILKGDISPEYVRHYLLGSTEYAESKASGTTFKELSGSRATELALPLPPLP
EQRRIVAKIDSLTAKSRRARDHLEHIPRLVEKYKQAILAAAFDGRLTELSPHDIVHPELGELIEFGPQNGLYLPKDRYGE
GTPILRIQNYGFNFIDEPTNWHRVTVSDAIAAQFAMSDGDLIINRVNSPSHLGKSMVVTKAMAGAIFESNMMRIRLNALA
EPKFVQLYLSSSQGRGSLTKDAKWAVNQASINQGDVSRTPVPLPGLSDQIAVLDRIETAFAWIDRLAAEATSARTLIDRL
DQAVLAKAFRGELVPQDPADEPASVLLERIRAERGAAPKARRGRRPAAEA
>Mature_449_residues
SEARWQVPHSWLWASFGEVADIVGGGTPPTGDEANFTKQGVPWLTPADLTGYRETYISRGRRDLSEKGYRESAARLLPKG
TVLFSSRAPVGYCAIASENVSTNQGFKSFILKGDISPEYVRHYLLGSTEYAESKASGTTFKELSGSRATELALPLPPLPE
QRRIVAKIDSLTAKSRRARDHLEHIPRLVEKYKQAILAAAFDGRLTELSPHDIVHPELGELIEFGPQNGLYLPKDRYGEG
TPILRIQNYGFNFIDEPTNWHRVTVSDAIAAQFAMSDGDLIINRVNSPSHLGKSMVVTKAMAGAIFESNMMRIRLNALAE
PKFVQLYLSSSQGRGSLTKDAKWAVNQASINQGDVSRTPVPLPGLSDQIAVLDRIETAFAWIDRLAAEATSARTLIDRLD
QAVLAKAFRGELVPQDPADEPASVLLERIRAERGAAPKARRGRRPAAEA

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=472, Percent_Identity=26.0593220338983, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 49458; Mature: 49326

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEARWQVPHSWLWASFGEVADIVGGGTPPTGDEANFTKQGVPWLTPADLTGYRETYISR
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
GRRDLSEKGYRESAARLLPKGTVLFSSRAPVGYCAIASENVSTNQGFKSFILKGDISPEY
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHHEEECCCCHHH
VRHYLLGSTEYAESKASGTTFKELSGSRATELALPLPPLPEQRRIVAKIDSLTAKSRRAR
HHHHHHCCCHHHHHCCCCCCHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
DHLEHIPRLVEKYKQAILAAAFDGRLTELSPHDIVHPELGELIEFGPQNGLYLPKDRYGE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCEECCCCCCCC
GTPILRIQNYGFNFIDEPTNWHRVTVSDAIAAQFAMSDGDLIINRVNSPSHLGKSMVVTK
CCCEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
AMAGAIFESNMMRIRLNALAEPKFVQLYLSSSQGRGSLTKDAKWAVNQASINQGDVSRTP
HHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCC
VPLPGLSDQIAVLDRIETAFAWIDRLAAEATSARTLIDRLDQAVLAKAFRGELVPQDPAD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EPASVLLERIRAERGAAPKARRGRRPAAEA
CHHHHHHHHHHHHCCCCCCHHCCCCCCCCC
>Mature Secondary Structure 
SEARWQVPHSWLWASFGEVADIVGGGTPPTGDEANFTKQGVPWLTPADLTGYRETYISR
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
GRRDLSEKGYRESAARLLPKGTVLFSSRAPVGYCAIASENVSTNQGFKSFILKGDISPEY
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHHEEECCCCHHH
VRHYLLGSTEYAESKASGTTFKELSGSRATELALPLPPLPEQRRIVAKIDSLTAKSRRAR
HHHHHHCCCHHHHHCCCCCCHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
DHLEHIPRLVEKYKQAILAAAFDGRLTELSPHDIVHPELGELIEFGPQNGLYLPKDRYGE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCEECCCCCCCC
GTPILRIQNYGFNFIDEPTNWHRVTVSDAIAAQFAMSDGDLIINRVNSPSHLGKSMVVTK
CCCEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
AMAGAIFESNMMRIRLNALAEPKFVQLYLSSSQGRGSLTKDAKWAVNQASINQGDVSRTP
HHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCC
VPLPGLSDQIAVLDRIETAFAWIDRLAAEATSARTLIDRLDQAVLAKAFRGELVPQDPAD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EPASVLLERIRAERGAAPKARRGRRPAAEA
CHHHHHHHHHHHHCCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6304321; 7610040; 9278503; 7739896 [H]