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The map label for this gene is pobA [H]

Identifier: 154244994

GI number: 154244994

Start: 1196665

End: 1198029

Strand: Direct

Name: pobA [H]

Synonym: Xaut_1044

Alternate gene names: 154244994

Gene position: 1196665-1198029 (Clockwise)

Preceding gene: 154244992

Following gene: 154244995

Centisome position: 22.54

GC content: 64.76

Gene sequence:

>1365_bases
ATGGCCCAGCAAAAGCAGTTTCTCTCGCAGCCCTTCGCCGGATATCTGAACGAGCCGGCACCTGAGGAGAATGTCGAGCT
CACCCACATTGAGAAGGGCTCGGCAGCCGGCGAGTTGCTGCGACGCTATTGGCAGCCGGTGGCCCTGACCAGCGAGATCA
AGGATGTGCCGCTCGGCATCCGGATGTTTGGTGAGGACCTCGTGGTCTTCCGCAACACGGCTGGCGAATACGGCATCCTC
GACCGCCATTGTCCGCACCGCGGGACGTCGCTGGAATTCGGCCTGCCCACCGAATGCGGCCTGCGGTGCTGCTACCATGG
CTGGCTCTTCGGCATGGATGGCAAGGTTCTGGAAACGCCCGGCGACCCGCCCGGCAGCACGCTGAAGGACCGGCTCTACC
ACGCCGCCTATCCTGCACGCGAATACAAGGGCCTCGTCTTCGGATACTTCGGCCCGATCGAGGCCATGCCGGAATTTCCC
ATCTACGATTCCTACGAGCACCCCGACGACAAGCTGGTGCCGTATTGCGTCACCTATCCCTGCAACTGGCTGCAGGTGCT
GGAAAACGTGATGGACCCGGCGCATGCGGTGTTCTTGCACACCCGCGTGACCTTCTCCCACTTCTCCGACACCTGGGGCG
AGCTTCCGGTGATGGATTTCGTGGAGACGCCCACCGGCATGATCTACGTCACCACCCGCCGCTGGAAGGACAAGGTGTGG
GTGCGATCCAACGACATCCTCATGCCGAACCTCGCCCAGGTCGGCCATATCTGGGAGGATGGGCAGGAGGCCAAGCAGTT
CGGCCGCGTGGGCATCACGCGCTGGACCACGCCCATCGACAACCAGACCTGCCGGGTGATCGGCTGGCGCCATTTCCATC
CGGACGTGGATCCGCGCGGCCTGGCGAAGGAAGAGGAGTGCGGGGTCGAGAAGGTGGATTTCTACGGACAGGGCATCGGG
GCCTCCTTCGAGGAGCGCCAGCGCCTGCCGGGAGACTATGACGCCATCGTTTCCCAGCGGCCCATCGCCGTCCACGCGCT
GGAGCACCTCACCTATTGCGACAAGGGCGTGGTGATGCTGCGCAAGCTCCTGCGCCGCGACATCCGCGGCCTTGCCGCAG
GCGAAGAGCGGGCTGCATCCCCGCTGCGCTCCAACGGCCTCATCCCGACCTACTGCCACGACACGGTTCTCGACATTCCG
CCCATCGACGGCGTGGACGATCTGGAGTTGCTCGAGGAGGTGGGCCGCCGCGTCACCGAGATCATCATCGAGGGCGAGCA
CCACAGCGCCGACGACCGGATCGAGATCGTGCGGCGCAAGCTGCTCGAATACCAGCGCTCGCTGCTGCCCCGCGCGGCGG
AGTGA

Upstream 100 bases:

>100_bases
ACCTAAAGAGCCGGCGCAATGCTCGTGTCATCCAAAGCGGAGCCGCTTCCGCGAATGGAAGCACCGCCCCACCTCCGCCG
CCCCACGGAGAGCAAAACGC

Downstream 100 bases:

>100_bases
ACGCACCCGGCGGCTTGCCCAGCCGCCGCCCCACGCGGCCACACAGCAATGCCGCAAGACCAGTGAGGCGAAACGGCCTC
GCAGCCATACAGCACAAGGA

Product: phthalate 4,5-dioxygenase

Products: NAD; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol [C]

Alternate protein names: 4-carboxydiphenyl ether; phenoxybenzoate dioxygenase [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MAQQKQFLSQPFAGYLNEPAPEENVELTHIEKGSAAGELLRRYWQPVALTSEIKDVPLGIRMFGEDLVVFRNTAGEYGIL
DRHCPHRGTSLEFGLPTECGLRCCYHGWLFGMDGKVLETPGDPPGSTLKDRLYHAAYPAREYKGLVFGYFGPIEAMPEFP
IYDSYEHPDDKLVPYCVTYPCNWLQVLENVMDPAHAVFLHTRVTFSHFSDTWGELPVMDFVETPTGMIYVTTRRWKDKVW
VRSNDILMPNLAQVGHIWEDGQEAKQFGRVGITRWTTPIDNQTCRVIGWRHFHPDVDPRGLAKEEECGVEKVDFYGQGIG
ASFEERQRLPGDYDAIVSQRPIAVHALEHLTYCDKGVVMLRKLLRRDIRGLAAGEERAASPLRSNGLIPTYCHDTVLDIP
PIDGVDDLELLEEVGRRVTEIIIEGEHHSADDRIEIVRRKLLEYQRSLLPRAAE

Sequences:

>Translated_454_residues
MAQQKQFLSQPFAGYLNEPAPEENVELTHIEKGSAAGELLRRYWQPVALTSEIKDVPLGIRMFGEDLVVFRNTAGEYGIL
DRHCPHRGTSLEFGLPTECGLRCCYHGWLFGMDGKVLETPGDPPGSTLKDRLYHAAYPAREYKGLVFGYFGPIEAMPEFP
IYDSYEHPDDKLVPYCVTYPCNWLQVLENVMDPAHAVFLHTRVTFSHFSDTWGELPVMDFVETPTGMIYVTTRRWKDKVW
VRSNDILMPNLAQVGHIWEDGQEAKQFGRVGITRWTTPIDNQTCRVIGWRHFHPDVDPRGLAKEEECGVEKVDFYGQGIG
ASFEERQRLPGDYDAIVSQRPIAVHALEHLTYCDKGVVMLRKLLRRDIRGLAAGEERAASPLRSNGLIPTYCHDTVLDIP
PIDGVDDLELLEEVGRRVTEIIIEGEHHSADDRIEIVRRKLLEYQRSLLPRAAE
>Mature_453_residues
AQQKQFLSQPFAGYLNEPAPEENVELTHIEKGSAAGELLRRYWQPVALTSEIKDVPLGIRMFGEDLVVFRNTAGEYGILD
RHCPHRGTSLEFGLPTECGLRCCYHGWLFGMDGKVLETPGDPPGSTLKDRLYHAAYPAREYKGLVFGYFGPIEAMPEFPI
YDSYEHPDDKLVPYCVTYPCNWLQVLENVMDPAHAVFLHTRVTFSHFSDTWGELPVMDFVETPTGMIYVTTRRWKDKVWV
RSNDILMPNLAQVGHIWEDGQEAKQFGRVGITRWTTPIDNQTCRVIGWRHFHPDVDPRGLAKEEECGVEKVDFYGQGIGA
SFEERQRLPGDYDAIVSQRPIAVHALEHLTYCDKGVVMLRKLLRRDIRGLAAGEERAASPLRSNGLIPTYCHDTVLDIPP
IDGVDDLELLEEVGRRVTEIIIEGEHHSADDRIEIVRRKLLEYQRSLLPRAAE

Specific function: Degrades exclusively diarylether compounds having carboxyl groups in the 3- or 4-position. Yields a hemiacetal that spontaneously hydrolyzes to phenol and protocatechuate [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881 [H]

Pfam domain/function: PF00355 Rieske [H]

EC number: 1.14.1.- [C]

Molecular weight: Translated: 51688; Mature: 51557

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQQKQFLSQPFAGYLNEPAPEENVELTHIEKGSAAGELLRRYWQPVALTSEIKDVPLGI
CCCHHHHHHCCHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCC
RMFGEDLVVFRNTAGEYGILDRHCPHRGTSLEFGLPTECGLRCCYHGWLFGMDGKVLETP
EECCCCEEEEECCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCEEECCCCEEEECC
GDPPGSTLKDRLYHAAYPAREYKGLVFGYFGPIEAMPEFPIYDSYEHPDDKLVPYCVTYP
CCCCCCHHHHHHHHHHCCHHHHCCEEEEECCCHHCCCCCCCCCCCCCCCCCCCCEEECCC
CNWLQVLENVMDPAHAVFLHTRVTFSHFSDTWGELPVMDFVETPTGMIYVTTRRWKDKVW
CCHHHHHHHHHCCHHEEEEEEHHHHHHHHCCCCCCCHHHHHCCCCCEEEEEECCCCCEEE
VRSNDILMPNLAQVGHIWEDGQEAKQFGRVGITRWTTPIDNQTCRVIGWRHFHPDVDPRG
EECCCCCCCCHHHHHHHHCCCHHHHHHCCCCCEECCCCCCCCCEEEEEEEECCCCCCCCC
LAKEEECGVEKVDFYGQGIGASFEERQRLPGDYDAIVSQRPIAVHALEHLTYCDKGVVML
CCCHHHCCCEEEHHCCCCCCCCHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHCCHHHHHH
RKLLRRDIRGLAAGEERAASPLRSNGLIPTYCHDTVLDIPPIDGVDDLELLEEVGRRVTE
HHHHHHHHHHCCCCHHHHCCCHHHCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHH
IIIEGEHHSADDRIEIVRRKLLEYQRSLLPRAAE
HEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AQQKQFLSQPFAGYLNEPAPEENVELTHIEKGSAAGELLRRYWQPVALTSEIKDVPLGI
CCHHHHHHCCHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCC
RMFGEDLVVFRNTAGEYGILDRHCPHRGTSLEFGLPTECGLRCCYHGWLFGMDGKVLETP
EECCCCEEEEECCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCEEECCCCEEEECC
GDPPGSTLKDRLYHAAYPAREYKGLVFGYFGPIEAMPEFPIYDSYEHPDDKLVPYCVTYP
CCCCCCHHHHHHHHHHCCHHHHCCEEEEECCCHHCCCCCCCCCCCCCCCCCCCCEEECCC
CNWLQVLENVMDPAHAVFLHTRVTFSHFSDTWGELPVMDFVETPTGMIYVTTRRWKDKVW
CCHHHHHHHHHCCHHEEEEEEHHHHHHHHCCCCCCCHHHHHCCCCCEEEEEECCCCCEEE
VRSNDILMPNLAQVGHIWEDGQEAKQFGRVGITRWTTPIDNQTCRVIGWRHFHPDVDPRG
EECCCCCCCCHHHHHHHHCCCHHHHHHCCCCCEECCCCCCCCCEEEEEEEECCCCCCCCC
LAKEEECGVEKVDFYGQGIGASFEERQRLPGDYDAIVSQRPIAVHALEHLTYCDKGVVML
CCCHHHCCCEEEHHCCCCCCCCHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHCCHHHHHH
RKLLRRDIRGLAAGEERAASPLRSNGLIPTYCHDTVLDIPPIDGVDDLELLEEVGRRVTE
HHHHHHHHHHCCCCHHHHCCCHHHCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHH
IIIEGEHHSADDRIEIVRRKLLEYQRSLLPRAAE
HEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2Fe-2S. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; Proton; O2; 3-phenylpropionate [C]

Specific reaction: 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD 3-phenylpropionate + NADH + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7710319 [H]