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The map label for this gene is yfcG [H]

Identifier: 154244930

GI number: 154244930

Start: 1095787

End: 1096500

Strand: Reverse

Name: yfcG [H]

Synonym: Xaut_0980

Alternate gene names: 154244930

Gene position: 1096500-1095787 (Counterclockwise)

Preceding gene: 154244933

Following gene: 154244929

Centisome position: 20.65

GC content: 63.03

Gene sequence:

>714_bases
ATGATCGATCTTTATTATTGGCCGACACCGAACGGCCACAAGATCACGCTTTTCCTGGAAGAGGCAGGGCTCGACTATCG
GATCGTTCCGGTGGACATCAGCGCGGGCGACCAGTTCAAGCCGGACTTTCTTGCGATTGCGCCGAACAACCGTATGCCGG
CCATCGTCGATCACACCCCGACTGACAAGGGCGCGCCGATCTCGGTGTTCGAATCCGGTGCCATCCTGCTTTATCTCGCC
GAGAAGACGGGGCGTTTCATCCCGTCCGACCTGCGCGGCCGCACCAGCGTGACGGAATGGCTGTTCTGGCAGATGGGCGG
GCTTGGCCCCATGGCCGGGCAGAACCACCATTTCAGCCAGTATGCACCGGAGAAGATCCCCTACTCCATCGAGCGCTACG
TGAAGGAAACCAACCGCCTCTATGGCGTGCTCGACCGTCGGCTGGCAGCGAATGCCGAGACCGGGAGCGCGTTTGTCGGT
GGCGCGGACTATTCCATCGCCGACATGGCCATCTATCCCTGGATCGTGCCCCACCAGCGCCAGAGCCAGGATCTTGCCGA
TTTCCCCCATCTCAAGCGCTGGTTCGAGGCGGTGGCGGCGCGACCGGCGACCATCGCCGCCTATGCCCGGGCCGAGCCTT
ATTCCAGCCGGCCGGCGGTGACCGAGGAAGGCAAGAAAATCCTCTTTGGCCAGACCGCTCGCCGCGCCGGGTGA

Upstream 100 bases:

>100_bases
ATAGAATGCACGGCAACGCGGGGACCGCAAGCTTCCTTCTGCGATTTTTGCGCGGTGTGAATGTTCTCGCGCGGCGCCCT
GCGCATCAACGAGGCAGACG

Downstream 100 bases:

>100_bases
CGACGCCGGGTGACGACAAGGCGGGCGGGCGTTAGGCTCGCCCACCAGTTCCAACCGCAACGAAAGTCCCCTTCACACAT
GGCACGGCCACGGGCGTTCG

Product: glutathione S-transferase domain-containing protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MIDLYYWPTPNGHKITLFLEEAGLDYRIVPVDISAGDQFKPDFLAIAPNNRMPAIVDHTPTDKGAPISVFESGAILLYLA
EKTGRFIPSDLRGRTSVTEWLFWQMGGLGPMAGQNHHFSQYAPEKIPYSIERYVKETNRLYGVLDRRLAANAETGSAFVG
GADYSIADMAIYPWIVPHQRQSQDLADFPHLKRWFEAVAARPATIAAYARAEPYSSRPAVTEEGKKILFGQTARRAG

Sequences:

>Translated_237_residues
MIDLYYWPTPNGHKITLFLEEAGLDYRIVPVDISAGDQFKPDFLAIAPNNRMPAIVDHTPTDKGAPISVFESGAILLYLA
EKTGRFIPSDLRGRTSVTEWLFWQMGGLGPMAGQNHHFSQYAPEKIPYSIERYVKETNRLYGVLDRRLAANAETGSAFVG
GADYSIADMAIYPWIVPHQRQSQDLADFPHLKRWFEAVAARPATIAAYARAEPYSSRPAVTEEGKKILFGQTARRAG
>Mature_237_residues
MIDLYYWPTPNGHKITLFLEEAGLDYRIVPVDISAGDQFKPDFLAIAPNNRMPAIVDHTPTDKGAPISVFESGAILLYLA
EKTGRFIPSDLRGRTSVTEWLFWQMGGLGPMAGQNHHFSQYAPEKIPYSIERYVKETNRLYGVLDRRLAANAETGSAFVG
GADYSIADMAIYPWIVPHQRQSQDLADFPHLKRWFEAVAARPATIAAYARAEPYSSRPAVTEEGKKILFGQTARRAG

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=212, Percent_Identity=59.4339622641509, Blast_Score=268, Evalue=3e-73,
Organism=Escherichia coli, GI87082195, Length=216, Percent_Identity=43.0555555555556, Blast_Score=166, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6324100, Length=232, Percent_Identity=32.7586206896552, Blast_Score=123, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17933730, Length=200, Percent_Identity=28, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17864592, Length=199, Percent_Identity=26.6331658291457, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28571670, Length=179, Percent_Identity=25.6983240223464, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI17864598, Length=207, Percent_Identity=27.0531400966184, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17864594, Length=202, Percent_Identity=26.7326732673267, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24646249, Length=204, Percent_Identity=26.9607843137255, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26461; Mature: 26461

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLYYWPTPNGHKITLFLEEAGLDYRIVPVDISAGDQFKPDFLAIAPNNRMPAIVDHTP
CEEEEECCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCEEEECCC
TDKGAPISVFESGAILLYLAEKTGRFIPSDLRGRTSVTEWLFWQMGGLGPMAGQNHHFSQ
CCCCCCEEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHH
YAPEKIPYSIERYVKETNRLYGVLDRRLAANAETGSAFVGGADYSIADMAIYPWIVPHQR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEECEECCCCC
QSQDLADFPHLKRWFEAVAARPATIAAYARAEPYSSRPAVTEEGKKILFGQTARRAG
CCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCHHCCC
>Mature Secondary Structure
MIDLYYWPTPNGHKITLFLEEAGLDYRIVPVDISAGDQFKPDFLAIAPNNRMPAIVDHTP
CEEEEECCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCEEEECCC
TDKGAPISVFESGAILLYLAEKTGRFIPSDLRGRTSVTEWLFWQMGGLGPMAGQNHHFSQ
CCCCCCEEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHH
YAPEKIPYSIERYVKETNRLYGVLDRRLAANAETGSAFVGGADYSIADMAIYPWIVPHQR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEECEECCCCC
QSQDLADFPHLKRWFEAVAARPATIAAYARAEPYSSRPAVTEEGKKILFGQTARRAG
CCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]