BacMap

Definition	Xanthobacter autotrophicus Py2 chromosome, complete genome.
Accession	NC_009720
Length	5,308,934

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The map label for this gene is echA8 [H]

Identifier: 154244858

GI number: 154244858

Start: 1019609

End: 1020385

Strand: Reverse

Name: echA8 [H]

Synonym: Xaut_0908

Alternate gene names: 154244858

Gene position: 1020385-1019609 (Counterclockwise)

Preceding gene: 154244860

Following gene: 154244853

Centisome position: 19.22

GC content: 66.67

Gene sequence:

>777_bases
ATGACCTATGAAACCATCCTCGTCGCGATCGACGGACCGGTCGGCATCATCACCCTCAACCGCCCGGACGTCCTCAACGC
GCTGAACCACCAAATCACCTCGGAGCTGTCTCATGCCGCGGCCGCCCTCGACGCTGCCGGCTCGATCGGCGCCATCGTTG
TGACCGGCTCGGACAAGGCCTTCGCCGCAGGCGCCGACATCAAGGAGATCAAGGACAAGACCTTTGCCGAAGTCTATGAA
GCGCAGCTCATCACCGCCACATGGGAGGGCTTGGCACGGGTGCGCAAGCCGACCGTCGCGGCGGTGTCGGGGCATGCGAT
CGGGGGCGGTTGCGAGCTGGCGCTGATGTGCGACATCATCGTGGCGTCGCAGACCGCCCGCTTCGCCCTGCCGGAAACCA
CGATTGGCATCATCCCCGGCGCCGGCGGCACCCAGCGCCTCACGCGCATCGTTGGCAAGGCGACCGCCATGGACATGATC
CTCACCGGCCGGGCCTTGTCCGCGGTGGAGGCGCAGGCCATGGGGCTCGTCAGCCGGGTGGCGGCAGAAGGGCAACATCT
GGCGGAGGCCGTCGCCATCGCCGCACGCATCGCCTCGTCCTCCCGCCCCGTCGTGGCGCTGGCGAAGGAAGCGGTGAACA
AGGCCTACGAGACCCATCTCCAGGAGGGCCTGTATGTCGAGCGCCGACTGCTCTACGCCACCTTCGCCCTAGAGGACCGG
CGCGAGGGGATGAACGCCTTTGCGGAAAAGCGAAAGCCGGTTTTCCGAAATCGGTGA

Upstream 100 bases:

>100_bases
CGCTGGCAGAGGCGGTATCCGCGTTGGCCCGCTTCCAAGGCCCGGCCCCGTAGGCGAGATCCTCATTCGCATTTCCTGAC
CAGAACCGAGGGCCACAGAC

Downstream 100 bases:

>100_bases
GCTGGGTGCCGGTGACCTGGCTGCTCGCGAATTGAGCGGTGCAGCTCTGCTCCGGTCTCGGTGCATCGAGATCGCGGAGC
GGATCGAGCTCCAAGGCACT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MTYETILVAIDGPVGIITLNRPDVLNALNHQITSELSHAAAALDAAGSIGAIVVTGSDKAFAAGADIKEIKDKTFAEVYE
AQLITATWEGLARVRKPTVAAVSGHAIGGGCELALMCDIIVASQTARFALPETTIGIIPGAGGTQRLTRIVGKATAMDMI
LTGRALSAVEAQAMGLVSRVAAEGQHLAEAVAIAARIASSSRPVVALAKEAVNKAYETHLQEGLYVERRLLYATFALEDR
REGMNAFAEKRKPVFRNR

Sequences:

>Translated_258_residues
MTYETILVAIDGPVGIITLNRPDVLNALNHQITSELSHAAAALDAAGSIGAIVVTGSDKAFAAGADIKEIKDKTFAEVYE
AQLITATWEGLARVRKPTVAAVSGHAIGGGCELALMCDIIVASQTARFALPETTIGIIPGAGGTQRLTRIVGKATAMDMI
LTGRALSAVEAQAMGLVSRVAAEGQHLAEAVAIAARIASSSRPVVALAKEAVNKAYETHLQEGLYVERRLLYATFALEDR
REGMNAFAEKRKPVFRNR
>Mature_257_residues
TYETILVAIDGPVGIITLNRPDVLNALNHQITSELSHAAAALDAAGSIGAIVVTGSDKAFAAGADIKEIKDKTFAEVYEA
QLITATWEGLARVRKPTVAAVSGHAIGGGCELALMCDIIVASQTARFALPETTIGIIPGAGGTQRLTRIVGKATAMDMIL
TGRALSAVEAQAMGLVSRVAAEGQHLAEAVAIAARIASSSRPVVALAKEAVNKAYETHLQEGLYVERRLLYATFALEDRR
EGMNAFAEKRKPVFRNR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=245, Percent_Identity=53.0612244897959, Blast_Score=268, Evalue=5e-72,
Organism=Homo sapiens, GI31542718, Length=253, Percent_Identity=32.8063241106719, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI4502327, Length=247, Percent_Identity=37.246963562753, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI20127408, Length=189, Percent_Identity=37.5661375661376, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI150378541, Length=244, Percent_Identity=34.0163934426229, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI68989263, Length=186, Percent_Identity=34.9462365591398, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI70995211, Length=213, Percent_Identity=31.924882629108, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI213417737, Length=226, Percent_Identity=27.8761061946903, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI157694516, Length=226, Percent_Identity=27.8761061946903, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI4757968, Length=257, Percent_Identity=27.6264591439689, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI49355787, Length=257, Percent_Identity=27.6264591439689, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI25453481, Length=210, Percent_Identity=30.952380952381, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI51538573, Length=210, Percent_Identity=30.952380952381, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI4757966, Length=210, Percent_Identity=30.952380952381, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI51538581, Length=210, Percent_Identity=30.952380952381, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI221307494, Length=209, Percent_Identity=31.1004784688995, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI221136756, Length=226, Percent_Identity=29.2035398230088, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI221136753, Length=226, Percent_Identity=29.2035398230088, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI37594469, Length=159, Percent_Identity=33.9622641509434, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI37594471, Length=186, Percent_Identity=31.7204301075269, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI45643119, Length=217, Percent_Identity=27.6497695852535, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI260275230, Length=217, Percent_Identity=27.6497695852535, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI260274832, Length=217, Percent_Identity=27.6497695852535, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI116235487, Length=204, Percent_Identity=29.4117647058824, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI261878539, Length=93, Percent_Identity=41.9354838709677, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI157694520, Length=186, Percent_Identity=27.9569892473118, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=43.75, Blast_Score=201, Evalue=3e-53,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=33.4600760456274, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=32.183908045977, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1788597, Length=246, Percent_Identity=32.9268292682927, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788682, Length=209, Percent_Identity=34.9282296650718, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=28.0769230769231, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790281, Length=170, Percent_Identity=32.9411764705882, Blast_Score=95, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=245, Percent_Identity=53.469387755102, Blast_Score=265, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI25145438, Length=242, Percent_Identity=51.2396694214876, Blast_Score=238, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI17560910, Length=244, Percent_Identity=33.1967213114754, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17540714, Length=227, Percent_Identity=34.3612334801762, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17536985, Length=225, Percent_Identity=32.4444444444444, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17558304, Length=189, Percent_Identity=36.5079365079365, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17508951, Length=242, Percent_Identity=32.6446280991736, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25144276, Length=246, Percent_Identity=32.520325203252, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17540306, Length=236, Percent_Identity=27.9661016949153, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17508953, Length=242, Percent_Identity=32.6446280991736, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=26.953125, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=224, Percent_Identity=30.3571428571429, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25144160, Length=224, Percent_Identity=30.3571428571429, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=240, Percent_Identity=28.75, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25141363, Length=171, Percent_Identity=28.6549707602339, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=198, Percent_Identity=28.2828282828283, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI20129971, Length=259, Percent_Identity=52.5096525096525, Blast_Score=268, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24653477, Length=259, Percent_Identity=52.5096525096525, Blast_Score=268, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24653139, Length=262, Percent_Identity=34.7328244274809, Blast_Score=137, Evalue=8e-33,
Organism=Drosophila melanogaster, GI19922422, Length=255, Percent_Identity=26.2745098039216, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21357171, Length=259, Percent_Identity=30.1158301158301, Blast_Score=107, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24650670, Length=228, Percent_Identity=32.4561403508772, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19920382, Length=271, Percent_Identity=30.2583025830258, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24654903, Length=182, Percent_Identity=27.4725274725275, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583165, Length=221, Percent_Identity=28.9592760180996, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19921000, Length=171, Percent_Identity=34.5029239766082, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24583077, Length=171, Percent_Identity=34.5029239766082, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24583079, Length=171, Percent_Identity=34.5029239766082, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI28571730, Length=175, Percent_Identity=30.8571428571429, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI28571729, Length=175, Percent_Identity=30.8571428571429, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19921018, Length=188, Percent_Identity=29.7872340425532, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27193; Mature: 27061

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYETILVAIDGPVGIITLNRPDVLNALNHQITSELSHAAAALDAAGSIGAIVVTGSDKA
CCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
FAAGADIKEIKDKTFAEVYEAQLITATWEGLARVRKPTVAAVSGHAIGGGCELALMCDII
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHH
VASQTARFALPETTIGIIPGAGGTQRLTRIVGKATAMDMILTGRALSAVEAQAMGLVSRV
HHCCCCCEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AAEGQHLAEAVAIAARIASSSRPVVALAKEAVNKAYETHLQEGLYVERRLLYATFALEDR
HHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
REGMNAFAEKRKPVFRNR
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TYETILVAIDGPVGIITLNRPDVLNALNHQITSELSHAAAALDAAGSIGAIVVTGSDKA
CCEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
FAAGADIKEIKDKTFAEVYEAQLITATWEGLARVRKPTVAAVSGHAIGGGCELALMCDII
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHH
VASQTARFALPETTIGIIPGAGGTQRLTRIVGKATAMDMILTGRALSAVEAQAMGLVSRV
HHCCCCCEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AAEGQHLAEAVAIAARIASSSRPVVALAKEAVNKAYETHLQEGLYVERRLLYATFALEDR
HHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
REGMNAFAEKRKPVFRNR
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]