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The map label for this gene is yafC [C]

Identifier: 154244831

GI number: 154244831

Start: 981832

End: 982782

Strand: Direct

Name: yafC [C]

Synonym: Xaut_0881

Alternate gene names: 154244831

Gene position: 981832-982782 (Clockwise)

Preceding gene: 154244829

Following gene: 154244833

Centisome position: 18.49

GC content: 68.35

Gene sequence:

>951_bases
ATGGTTGAATCGCCACATGGTGCGTGCAAAAAATCGCAGACCATGCGCGACCTCGACTGGAACGACCTGCAAGCCTTCCT
GGCCGTGGTGCGGGCCGGGCGGCTCACTGCCGCGGCCCAGGCCATGCGCGTGGATCATTCCACCCTGAGCCGGCGCATCA
CCGGGCTGGAGGCGGCGCTGAAGACCCGCCTGTTCGAGCGGCGCCCGGCCGGCTACGTGCCCACCGCCGCCGGGGAGCGG
CTGGTGGCGGAAGCGGAGGCGGTGGAAAGCATCGTCATCGGCATGCGCGCCAAGCTCACCGACCAGGTGCTGGGCCTCGC
GGGATCGGTGCGCATCGGCACCCCGGAGGGTTTCGGCACCTATTTCCTGACCCCGCAGATGATCCATTTCTGCGCCCGTC
ATCGCGAGCTGCAGGTGGAACTGGTGGCCAATCCGCGCACCGTCAGCCTGTCCAAGCGGGAGGCGGACATCGGCATCATG
ATGGCCCGCCCGGAAGAGGGGCGCCTGCATGCCACGCGGCTGACGGACTACGAGATGGGGCTCTATGCCTCCCGGCACTA
TCTGGCGCAGCACGCGCCCATCGCCAGCCGGGCCGACATCCGCCAGCACGCCTTCATCGGCTATATCGACGACCTGCTGC
CCACGGCCGCCCACGGCTATGTGGCTGAGCTGGACAAGGCGCTGAACCCGCAGATGCGCATGTCCAACATCATCACCCAG
CTGGTGGCGGCCACCAACGGGGGCGGGCTGTGCATGCTGCCATGCTTCATGGCCGGCACGTCGGCCGACCTCGTCCGGCT
GCTTCCGGAGGAGATCGCCATCACCCGGTCCTACTGGCTGGTGATCCATTCCGACGTGCGGGAGCTGGCGCGGGTGCGGG
CGGTCATCGACTTCATCGCCGAGACCGTGGCGGAGCAGCGGCGGCTGTTCCTGCCGTCCGGCTCCGCTTGA

Upstream 100 bases:

>100_bases
TGGCATAGAGATTCATGGCTTTCTCCCGTGTCGTTGGCATCGCGGTCTTTGGGCCGCGCGATCCGTCGCACAGGTGTAAG
GCGGGGTTTTCCCAATATAA

Downstream 100 bases:

>100_bases
AGATGAGCAGGGCGGGCGAGCGCGCGAGCGGGCTGTGAAACCCTGTTGGATATTCGCTCAGATGGGTGCCTGAAGCCGTC
CCAGGCGCATGATCGTCGTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MVESPHGACKKSQTMRDLDWNDLQAFLAVVRAGRLTAAAQAMRVDHSTLSRRITGLEAALKTRLFERRPAGYVPTAAGER
LVAEAEAVESIVIGMRAKLTDQVLGLAGSVRIGTPEGFGTYFLTPQMIHFCARHRELQVELVANPRTVSLSKREADIGIM
MARPEEGRLHATRLTDYEMGLYASRHYLAQHAPIASRADIRQHAFIGYIDDLLPTAAHGYVAELDKALNPQMRMSNIITQ
LVAATNGGGLCMLPCFMAGTSADLVRLLPEEIAITRSYWLVIHSDVRELARVRAVIDFIAETVAEQRRLFLPSGSA

Sequences:

>Translated_316_residues
MVESPHGACKKSQTMRDLDWNDLQAFLAVVRAGRLTAAAQAMRVDHSTLSRRITGLEAALKTRLFERRPAGYVPTAAGER
LVAEAEAVESIVIGMRAKLTDQVLGLAGSVRIGTPEGFGTYFLTPQMIHFCARHRELQVELVANPRTVSLSKREADIGIM
MARPEEGRLHATRLTDYEMGLYASRHYLAQHAPIASRADIRQHAFIGYIDDLLPTAAHGYVAELDKALNPQMRMSNIITQ
LVAATNGGGLCMLPCFMAGTSADLVRLLPEEIAITRSYWLVIHSDVRELARVRAVIDFIAETVAEQRRLFLPSGSA
>Mature_316_residues
MVESPHGACKKSQTMRDLDWNDLQAFLAVVRAGRLTAAAQAMRVDHSTLSRRITGLEAALKTRLFERRPAGYVPTAAGER
LVAEAEAVESIVIGMRAKLTDQVLGLAGSVRIGTPEGFGTYFLTPQMIHFCARHRELQVELVANPRTVSLSKREADIGIM
MARPEEGRLHATRLTDYEMGLYASRHYLAQHAPIASRADIRQHAFIGYIDDLLPTAAHGYVAELDKALNPQMRMSNIITQ
LVAATNGGGLCMLPCFMAGTSADLVRLLPEEIAITRSYWLVIHSDVRELARVRAVIDFIAETVAEQRRLFLPSGSA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=23.7113402061856, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789440, Length=314, Percent_Identity=25.4777070063694, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787589, Length=248, Percent_Identity=25.4032258064516, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI157672245, Length=175, Percent_Identity=33.1428571428571, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34805; Mature: 34805

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVESPHGACKKSQTMRDLDWNDLQAFLAVVRAGRLTAAAQAMRVDHSTLSRRITGLEAAL
CCCCCCCCHHHCCHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KTRLFERRPAGYVPTAAGERLVAEAEAVESIVIGMRAKLTDQVLGLAGSVRIGTPEGFGT
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCE
YFLTPQMIHFCARHRELQVELVANPRTVSLSKREADIGIMMARPEEGRLHATRLTDYEMG
EEECHHHHHHHHHCCCEEEEEEECCCEEEECCCCCCEEEEEECCCCCCEEEEEECCHHHH
LYASRHYLAQHAPIASRADIRQHAFIGYIDDLLPTAAHGYVAELDKALNPQMRMSNIITQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHH
LVAATNGGGLCMLPCFMAGTSADLVRLLPEEIAITRSYWLVIHSDVRELARVRAVIDFIA
HHHHCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHH
ETVAEQRRLFLPSGSA
HHHHHHHEECCCCCCC
>Mature Secondary Structure
MVESPHGACKKSQTMRDLDWNDLQAFLAVVRAGRLTAAAQAMRVDHSTLSRRITGLEAAL
CCCCCCCCHHHCCHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KTRLFERRPAGYVPTAAGERLVAEAEAVESIVIGMRAKLTDQVLGLAGSVRIGTPEGFGT
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCE
YFLTPQMIHFCARHRELQVELVANPRTVSLSKREADIGIMMARPEEGRLHATRLTDYEMG
EEECHHHHHHHHHCCCEEEEEEECCCEEEECCCCCCEEEEEECCCCCCEEEEEECCHHHH
LYASRHYLAQHAPIASRADIRQHAFIGYIDDLLPTAAHGYVAELDKALNPQMRMSNIITQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHH
LVAATNGGGLCMLPCFMAGTSADLVRLLPEEIAITRSYWLVIHSDVRELARVRAVIDFIA
HHHHCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHH
ETVAEQRRLFLPSGSA
HHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]