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The map label for this gene is potH [H]

Identifier: 154244814

GI number: 154244814

Start: 962566

End: 963492

Strand: Direct

Name: potH [H]

Synonym: Xaut_0864

Alternate gene names: 154244814

Gene position: 962566-963492 (Clockwise)

Preceding gene: 154244813

Following gene: 154244815

Centisome position: 18.13

GC content: 67.31

Gene sequence:

>927_bases
ATGGCGAGCGATGCCTCTCTCCCATCCTCCCTCGCGGATCCACGGGGCAAGTGGATGTCATTGAAAAACCTCGGTCGCCA
GGGTTTCGGCCGGCTCGGATGGTGGGGGCTTCTGGCCCCCGCCATGGCGTTGTACGCCCTCGTCTTCTTCATGCCGCTGG
GCGTGCTGGTGGGCGAGAGCGTGCACCCCTCCGGCGCGCCGCAGCTGTCGCTTGCCAATTATGCCGCCTTCTTCACCGAT
GGCGTCACCTTCTCCATCTTCGCGCGCACGGTGCGGCTTGCCTTCTTCGTCACCCTCACCTGCCTCGTGTTCGGCTATCC
GCTGGCCCTGTTCATGCGGCGGGCGGGGCCGGTGCTGCGGCTGGTGGCGCTCACCATCGTGGTGTCGCCGCTGCTCACCT
CGGTCATCGTGCGCAATGTGGCCTGGCTGCTGGTCCTGGGGCGCGAGGGCATGGTGAACACCCTGCTGCGGCAGGCCGGC
CTCATCGATGCGCCGCTGCCACTGCTCTACAACACCTTCGGCGTGGTGGTGGGCGTCACCCACGTCTATCTCGCCTTCCT
GGTGCTGCCGGTGTTCTCCTCGCTGCTCGCCATCGATCCCTCGGTGGAACAGGCGGCGTCGAGCCTTGGCGCCTCGCGCT
TCACCGTGTTCCGCAAGGTGACGGTGCCGCTCTCTTTGCCGGGGGTGATCGCGGGGTCCACCCTCGTGTTCGTGCTCACC
ATGGGGGTGTATCTCACGCCGGTCATCATGGGCGGCAGCTTCGTCACCACGCTGCCCATGGTGATGACGGACCTGGTACG
CACCCAGTTCGACTGGAGCCGCGCGGCGGCCTTCGCCATCGTGCTCCTGGCCTTCATCGCCGTGGTGATGGTGGCGTCCA
GCCGCGCCGAGCGGCGCATCGAGCGCATGCGCGGGGAGCTGTCGTGA

Upstream 100 bases:

>100_bases
GGAGGATTTCGAGGTGATCGTGGACAATCTGCCCGATTGGCGCCGGCGCTGGGATGCCTGGAAGCAGAGCTGAGCGAAAG
CCGGTCGCGGAGCCCTCGTC

Downstream 100 bases:

>100_bases
TGAGCGAGAACCGGATCGGCCTCCTCGTGGGTACCATCGCCGTGACGGTGATGGTGTTCCTCATCCTGCCCCAGCTCATC
CTGCTGGTGCAGTCGTTCAC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MASDASLPSSLADPRGKWMSLKNLGRQGFGRLGWWGLLAPAMALYALVFFMPLGVLVGESVHPSGAPQLSLANYAAFFTD
GVTFSIFARTVRLAFFVTLTCLVFGYPLALFMRRAGPVLRLVALTIVVSPLLTSVIVRNVAWLLVLGREGMVNTLLRQAG
LIDAPLPLLYNTFGVVVGVTHVYLAFLVLPVFSSLLAIDPSVEQAASSLGASRFTVFRKVTVPLSLPGVIAGSTLVFVLT
MGVYLTPVIMGGSFVTTLPMVMTDLVRTQFDWSRAAAFAIVLLAFIAVVMVASSRAERRIERMRGELS

Sequences:

>Translated_308_residues
MASDASLPSSLADPRGKWMSLKNLGRQGFGRLGWWGLLAPAMALYALVFFMPLGVLVGESVHPSGAPQLSLANYAAFFTD
GVTFSIFARTVRLAFFVTLTCLVFGYPLALFMRRAGPVLRLVALTIVVSPLLTSVIVRNVAWLLVLGREGMVNTLLRQAG
LIDAPLPLLYNTFGVVVGVTHVYLAFLVLPVFSSLLAIDPSVEQAASSLGASRFTVFRKVTVPLSLPGVIAGSTLVFVLT
MGVYLTPVIMGGSFVTTLPMVMTDLVRTQFDWSRAAAFAIVLLAFIAVVMVASSRAERRIERMRGELS
>Mature_307_residues
ASDASLPSSLADPRGKWMSLKNLGRQGFGRLGWWGLLAPAMALYALVFFMPLGVLVGESVHPSGAPQLSLANYAAFFTDG
VTFSIFARTVRLAFFVTLTCLVFGYPLALFMRRAGPVLRLVALTIVVSPLLTSVIVRNVAWLLVLGREGMVNTLLRQAGL
IDAPLPLLYNTFGVVVGVTHVYLAFLVLPVFSSLLAIDPSVEQAASSLGASRFTVFRKVTVPLSLPGVIAGSTLVFVLTM
GVYLTPVIMGGSFVTTLPMVMTDLVRTQFDWSRAAAFAIVLLAFIAVVMVASSRAERRIERMRGELS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=231, Percent_Identity=32.9004329004329, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1787369, Length=237, Percent_Identity=29.535864978903, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787713, Length=245, Percent_Identity=30.6122448979592, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33176; Mature: 33045

Theoretical pI: Translated: 11.05; Mature: 11.05

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDASLPSSLADPRGKWMSLKNLGRQGFGRLGWWGLLAPAMALYALVFFMPLGVLVGES
CCCCCCCCCHHCCCCHHHHHHHHHCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VHPSGAPQLSLANYAAFFTDGVTFSIFARTVRLAFFVTLTCLVFGYPLALFMRRAGPVLR
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LVALTIVVSPLLTSVIVRNVAWLLVLGREGMVNTLLRQAGLIDAPLPLLYNTFGVVVGVT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
HVYLAFLVLPVFSSLLAIDPSVEQAASSLGASRFTVFRKVTVPLSLPGVIAGSTLVFVLT
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHEECCCCCCCHHHHHHHHHHHH
MGVYLTPVIMGGSFVTTLPMVMTDLVRTQFDWSRAAAFAIVLLAFIAVVMVASSRAERRI
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERMRGELS
HHHHCCCC
>Mature Secondary Structure 
ASDASLPSSLADPRGKWMSLKNLGRQGFGRLGWWGLLAPAMALYALVFFMPLGVLVGES
CCCCCCCCHHCCCCHHHHHHHHHCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VHPSGAPQLSLANYAAFFTDGVTFSIFARTVRLAFFVTLTCLVFGYPLALFMRRAGPVLR
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LVALTIVVSPLLTSVIVRNVAWLLVLGREGMVNTLLRQAGLIDAPLPLLYNTFGVVVGVT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
HVYLAFLVLPVFSSLLAIDPSVEQAASSLGASRFTVFRKVTVPLSLPGVIAGSTLVFVLT
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHEECCCCCCCHHHHHHHHHHHH
MGVYLTPVIMGGSFVTTLPMVMTDLVRTQFDWSRAAAFAIVLLAFIAVVMVASSRAERRI
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERMRGELS
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]