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The map label for this gene is hsdM [H]

Identifier: 154244736

GI number: 154244736

Start: 877832

End: 879964

Strand: Direct

Name: hsdM [H]

Synonym: Xaut_0786

Alternate gene names: 154244736

Gene position: 877832-879964 (Clockwise)

Preceding gene: 154244735

Following gene: 154244737

Centisome position: 16.53

GC content: 57.81

Gene sequence:

>2133_bases
ATGCTGCCACGTGAAAAGAAGAAGGATAAGGTGCCGAAGACAACGGCTCAGCGACTCGACAGCGTCATCAAGTCTGCGCG
CAACAAGATGCGCAAAGACCGCGAGCTGAAGACGGACCTCACGCGGCTTCCTACCCTGACGTGGCTGATGTTCCTGAAGT
TCGTGGACGATATGGAACGGGTCCACGAGGACGAGGCCGTTCTCGCCGGCGAGACCTATCGAAGTTTGATCGACCCTCCC
TATCGGTGGCGCGACTGGGCCTCCGATTCCGCAGGCATCACGGGACCGGATCTGCTCTCGTTCATCAATGGGGAGCAGAC
CGTCCGAGCCGACGGAACAAAGGGGCCCGGCTTGTTCACGTATCTGCGGAGTCTTCGCGGGAATGGGGAAGGGCGACAGC
GCCGTGACGTTGTTTCAGCCGTGTTCCGCGACCTGCGCAACTACGCGACGAGCGGCTACGTGCTGCGGGACGTCATCAAC
CTCGTAAACGACATCCACTTCGACAGCACCGAAGACATCCAGACGCTTGGCCGGATGTATGAAACGCTGCTGCGGGAAAT
GCGGGACGCGGCGGGTCAGAACGGAGAGTTCTATACCCCGCGACCTGTCGTGCGCTTCATGGTCCAGGTCATCGACCCGA
AGCTGAGCGAAACGGTTCTCGATCCGGCTTGCGGCACCGGAGGGTTTCTCGCCGCAGCCTTCGATCACATGAAGCCCAGC
GCCGACACGGTGGAGAAGCGCGAGATCCTTCAGCGCTCGACGTTGCGCGGCGGCGAGGACTCGTCGCTACCGTTTCTGTT
GGCACAGATGAATCTGCTGCTGCACGGGTTGGAGGCGCCCGACATCGAGTTCGGAAACGCGCTGCGTTTCAAGCTCACAG
AGATCGGTGAGCGTGACCGCGTCGAGGTGATCCTGACCAATCCTCCGTTCGGCGGAGAGGAGGAAGCAGGCATCCTCACG
AACTTCCCGGATGATCGCCGGACGGCCGAGACCGCTCTGCTGTTTCTGCAACTCATTATGCGACGGCTGAAGCGAGGAGG
CCACGGGCGAGCCGGCGTAGTCGTTCCGAATGGGATATTGTTTGGCGATGGCATCGCGGCGCGCATCAAGGCGGACCTGC
TAGAGCAGTTCAATTTGCACACTATCGTCCGGCTGCCGGAAGGAACGTTTGCCCCCTATACCGATATTCCGACCAACATC
ATTTTCTTCGACACTTCAGGCCCGACGGGTGACATTTGGTATTGGGAACAGCCGCTCCCAGGGGGGCGGCGCAAGTATTC
AAAAACGCAGCCATTACTGTACGAAGAACTCACTGACTGCCTTGAGTGGTGGTCCGCGCGCAAGGAAGGACCACAAGCAT
GGAAAGTCTTTGGGCCTAGTCTCATCAAGCGCGATACAGAAGGTCGCACAATCGCGGTGGATTTGGACCTCAAGAATCCT
CATGCCAAGGAGGCGAACCACCGTCGACCTGTTGAGATCATCGAAAGCGCAGTCGCCAAGCAGCGTGAGTTGCTAGCTGT
GCTTGACGAGTTGAGAGCTCTGGTAGGAGCATTCGACAAGATCACTTCCAACTCACCTCGCGCAAAACTCGGCGACATCG
CGCCACTTGTTCGACGTCATGTTCAAATCGATCCTGAGAAGACCTATACTGAAATTGGTGTGCGCAGCTTCTACAAGGGG
ATATTCCATCGCCGGACAATACCAGGGGCGGAGTTCACTTGGCAGAAGTTATTCCGCATCGCCACCGGTGACCTCGTATT
CAGTAATTTGATGGCGTGGGAACAGGCAATAGCGCTGGCGTCAACTGCTGATGATGGCTGCGTCGGAAACCATCGGATGC
TTACGTGTGAAGCCGATCGAACGAGATGTCTCCCGATGTTTCTCTGGTACTATTTCCGTACTCCTGAAGGTTTTGCGCAG
GTTGTCGCGGCGTCACCGGGATCGATCGCACGAAATAAGACGCTGTCAGCCGAACTCTTGCCCAACATCACCGTTCCGGT
CCCGTCGCTGGACGCGCAGGAGTGGTTTGAAGCCCTTCATGCGAAAGCTCATGCAGTCGAACTTGGTCAAGCCGAGGTTG
ATCGAGTGCTAAGCGGGCTGATGCCATCCTTACTGCACCATACGTTCGGTTGA

Upstream 100 bases:

>100_bases
CCAGTGAAATCGCGCGACGCTTCGGAGGTCCGGACAAGCTTCGCGCCGCCGTGAATGAAATGTCCGGCCTCTTGTACGCC
GCCTAGGAAAGCACCTCTGA

Downstream 100 bases:

>100_bases
CGCGTGGCGCGTTCGGTCGTGGATGGCGAGGTGCAGCGACGCTCGGGAGAGATATGCGCGCGGTGCAGACGATATCGGGA
GCTGAAGCGAGGGCGCTGAT

Product: N-6 DNA methylase

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 710; Mature: 710

Protein sequence:

>710_residues
MLPREKKKDKVPKTTAQRLDSVIKSARNKMRKDRELKTDLTRLPTLTWLMFLKFVDDMERVHEDEAVLAGETYRSLIDPP
YRWRDWASDSAGITGPDLLSFINGEQTVRADGTKGPGLFTYLRSLRGNGEGRQRRDVVSAVFRDLRNYATSGYVLRDVIN
LVNDIHFDSTEDIQTLGRMYETLLREMRDAAGQNGEFYTPRPVVRFMVQVIDPKLSETVLDPACGTGGFLAAAFDHMKPS
ADTVEKREILQRSTLRGGEDSSLPFLLAQMNLLLHGLEAPDIEFGNALRFKLTEIGERDRVEVILTNPPFGGEEEAGILT
NFPDDRRTAETALLFLQLIMRRLKRGGHGRAGVVVPNGILFGDGIAARIKADLLEQFNLHTIVRLPEGTFAPYTDIPTNI
IFFDTSGPTGDIWYWEQPLPGGRRKYSKTQPLLYEELTDCLEWWSARKEGPQAWKVFGPSLIKRDTEGRTIAVDLDLKNP
HAKEANHRRPVEIIESAVAKQRELLAVLDELRALVGAFDKITSNSPRAKLGDIAPLVRRHVQIDPEKTYTEIGVRSFYKG
IFHRRTIPGAEFTWQKLFRIATGDLVFSNLMAWEQAIALASTADDGCVGNHRMLTCEADRTRCLPMFLWYYFRTPEGFAQ
VVAASPGSIARNKTLSAELLPNITVPVPSLDAQEWFEALHAKAHAVELGQAEVDRVLSGLMPSLLHHTFG

Sequences:

>Translated_710_residues
MLPREKKKDKVPKTTAQRLDSVIKSARNKMRKDRELKTDLTRLPTLTWLMFLKFVDDMERVHEDEAVLAGETYRSLIDPP
YRWRDWASDSAGITGPDLLSFINGEQTVRADGTKGPGLFTYLRSLRGNGEGRQRRDVVSAVFRDLRNYATSGYVLRDVIN
LVNDIHFDSTEDIQTLGRMYETLLREMRDAAGQNGEFYTPRPVVRFMVQVIDPKLSETVLDPACGTGGFLAAAFDHMKPS
ADTVEKREILQRSTLRGGEDSSLPFLLAQMNLLLHGLEAPDIEFGNALRFKLTEIGERDRVEVILTNPPFGGEEEAGILT
NFPDDRRTAETALLFLQLIMRRLKRGGHGRAGVVVPNGILFGDGIAARIKADLLEQFNLHTIVRLPEGTFAPYTDIPTNI
IFFDTSGPTGDIWYWEQPLPGGRRKYSKTQPLLYEELTDCLEWWSARKEGPQAWKVFGPSLIKRDTEGRTIAVDLDLKNP
HAKEANHRRPVEIIESAVAKQRELLAVLDELRALVGAFDKITSNSPRAKLGDIAPLVRRHVQIDPEKTYTEIGVRSFYKG
IFHRRTIPGAEFTWQKLFRIATGDLVFSNLMAWEQAIALASTADDGCVGNHRMLTCEADRTRCLPMFLWYYFRTPEGFAQ
VVAASPGSIARNKTLSAELLPNITVPVPSLDAQEWFEALHAKAHAVELGQAEVDRVLSGLMPSLLHHTFG
>Mature_710_residues
MLPREKKKDKVPKTTAQRLDSVIKSARNKMRKDRELKTDLTRLPTLTWLMFLKFVDDMERVHEDEAVLAGETYRSLIDPP
YRWRDWASDSAGITGPDLLSFINGEQTVRADGTKGPGLFTYLRSLRGNGEGRQRRDVVSAVFRDLRNYATSGYVLRDVIN
LVNDIHFDSTEDIQTLGRMYETLLREMRDAAGQNGEFYTPRPVVRFMVQVIDPKLSETVLDPACGTGGFLAAAFDHMKPS
ADTVEKREILQRSTLRGGEDSSLPFLLAQMNLLLHGLEAPDIEFGNALRFKLTEIGERDRVEVILTNPPFGGEEEAGILT
NFPDDRRTAETALLFLQLIMRRLKRGGHGRAGVVVPNGILFGDGIAARIKADLLEQFNLHTIVRLPEGTFAPYTDIPTNI
IFFDTSGPTGDIWYWEQPLPGGRRKYSKTQPLLYEELTDCLEWWSARKEGPQAWKVFGPSLIKRDTEGRTIAVDLDLKNP
HAKEANHRRPVEIIESAVAKQRELLAVLDELRALVGAFDKITSNSPRAKLGDIAPLVRRHVQIDPEKTYTEIGVRSFYKG
IFHRRTIPGAEFTWQKLFRIATGDLVFSNLMAWEQAIALASTADDGCVGNHRMLTCEADRTRCLPMFLWYYFRTPEGFAQ
VVAASPGSIARNKTLSAELLPNITVPVPSLDAQEWFEALHAKAHAVELGQAEVDRVLSGLMPSLLHHTFG

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=335, Percent_Identity=31.3432835820896, Blast_Score=128, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 79895; Mature: 79895

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPREKKKDKVPKTTAQRLDSVIKSARNKMRKDRELKTDLTRLPTLTWLMFLKFVDDMER
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHEDEAVLAGETYRSLIDPPYRWRDWASDSAGITGPDLLSFINGEQTVRADGTKGPGLFT
HCCCCHHHCCHHHHHHCCCCCCHHCCCCCCCCCCCHHHHHHHCCCCEEECCCCCCCHHHH
YLRSLRGNGEGRQRRDVVSAVFRDLRNYATSGYVLRDVINLVNDIHFDSTEDIQTLGRMY
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ETLLREMRDAAGQNGEFYTPRPVVRFMVQVIDPKLSETVLDPACGTGGFLAAAFDHMKPS
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHCCCC
ADTVEKREILQRSTLRGGEDSSLPFLLAQMNLLLHGLEAPDIEFGNALRFKLTEIGERDR
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHCCCCCC
VEVILTNPPFGGEEEAGILTNFPDDRRTAETALLFLQLIMRRLKRGGHGRAGVVVPNGIL
EEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCEE
FGDGIAARIKADLLEQFNLHTIVRLPEGTFAPYTDIPTNIIFFDTSGPTGDIWYWEQPLP
ECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCC
GGRRKYSKTQPLLYEELTDCLEWWSARKEGPQAWKVFGPSLIKRDTEGRTIAVDLDLKNP
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCHHHHHCCCCCCEEEEEEECCCC
HAKEANHRRPVEIIESAVAKQRELLAVLDELRALVGAFDKITSNSPRAKLGDIAPLVRRH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VQIDPEKTYTEIGVRSFYKGIFHRRTIPGAEFTWQKLFRIATGDLVFSNLMAWEQAIALA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STADDGCVGNHRMLTCEADRTRCLPMFLWYYFRTPEGFAQVVAASPGSIARNKTLSAELL
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHCCCCCHHHC
PNITVPVPSLDAQEWFEALHAKAHAVELGQAEVDRVLSGLMPSLLHHTFG
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLPREKKKDKVPKTTAQRLDSVIKSARNKMRKDRELKTDLTRLPTLTWLMFLKFVDDMER
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHEDEAVLAGETYRSLIDPPYRWRDWASDSAGITGPDLLSFINGEQTVRADGTKGPGLFT
HCCCCHHHCCHHHHHHCCCCCCHHCCCCCCCCCCCHHHHHHHCCCCEEECCCCCCCHHHH
YLRSLRGNGEGRQRRDVVSAVFRDLRNYATSGYVLRDVINLVNDIHFDSTEDIQTLGRMY
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ETLLREMRDAAGQNGEFYTPRPVVRFMVQVIDPKLSETVLDPACGTGGFLAAAFDHMKPS
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHCCCC
ADTVEKREILQRSTLRGGEDSSLPFLLAQMNLLLHGLEAPDIEFGNALRFKLTEIGERDR
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHCCCCCC
VEVILTNPPFGGEEEAGILTNFPDDRRTAETALLFLQLIMRRLKRGGHGRAGVVVPNGIL
EEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCEE
FGDGIAARIKADLLEQFNLHTIVRLPEGTFAPYTDIPTNIIFFDTSGPTGDIWYWEQPLP
ECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCC
GGRRKYSKTQPLLYEELTDCLEWWSARKEGPQAWKVFGPSLIKRDTEGRTIAVDLDLKNP
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCHHHHHCCCCCCEEEEEEECCCC
HAKEANHRRPVEIIESAVAKQRELLAVLDELRALVGAFDKITSNSPRAKLGDIAPLVRRH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VQIDPEKTYTEIGVRSFYKGIFHRRTIPGAEFTWQKLFRIATGDLVFSNLMAWEQAIALA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STADDGCVGNHRMLTCEADRTRCLPMFLWYYFRTPEGFAQVVAASPGSIARNKTLSAELL
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHCCCCCHHHC
PNITVPVPSLDAQEWFEALHAKAHAVELGQAEVDRVLSGLMPSLLHHTFG
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]