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The map label for this gene is fixL [H]

Identifier: 154244584

GI number: 154244584

Start: 711187

End: 713274

Strand: Direct

Name: fixL [H]

Synonym: Xaut_0632

Alternate gene names: 154244584

Gene position: 711187-713274 (Clockwise)

Preceding gene: 154244582

Following gene: 154244585

Centisome position: 13.4

GC content: 70.26

Gene sequence:

>2088_bases
ATGACATCGGCTCTCGACACGCAGGAGATCGTGCCGGACGGCGGCGGCGATGCGAGCCTTGTCCGGCTGTCCGACCTCGG
CCTGTTCACGGCGCTGTTCCAGTCCATCCCCGGCCGCGCGGTGCTGCTGGACAGCTCCGGCGTCTGCCGCTTCGCCAACC
AGGAGTTCATGGACTTTCTCGGGCGCAGCCGGGACGAGGTGATCGACCGGCCCGTGCAGGACTTCCTCGAGCCCAGCGTG
TTCGAGTCCTACGCCCCGCTGATGGAGAGGGTGTTCGGCGGCGAGCAGGTGCGGCGTGTGGCCTGGGTGGATTACGGCCG
CCTCGGCAAGCGATACCTGGAGGAGGTGATCTCGCCCTACCGGGTGGACCTCACCGGCCCGGTGGTCGGCTGCTTCGGCG
TCGGCCGCGACCTCACCGACATGAAACACCAGGAGATGGAGATCGTCCGGCGCACGCAGGCGCAGATGGAGGCCGAGGCC
TATCACGCCGCCATCGTCGGCACCGCCCTCGACGGCATCGTGGTGGTGGACGAAGAGGGCTCCGTGGTGGATTTCAACCC
GGCTGCCGAACAGATCTTCGGCTACCGGAAGGAGGAGGCCCTCGGCCGCTCCATCGCCGAGCTGCTGGTGCCGCCGGAAT
TGCGCGCCGCCCACGCGGCAGGCATGCGATCCTACCTCGCCACCGGCATCACCCGGCTGATCGGCCGGCGGCTGGAGCTG
CCGGCGCTGCTGGCTGACGGCACGCGCATCCCCATCGAGCTGGCCATCACCGACGTGACCCGTGGCGAGCGCCGGCTGTT
TGCCGCGCACATGCGCGACCTGCGCGCCGCCAAGCGGGCGGAGGAGGAGATCAAGCGGCAGCGCAACGCGCTCCACCAGA
AGGAGAAGCTTGCGGCGCTGGGCTCCCTGCTGGCCGGCGTCGCCCATGAACTGAACAACCCGCTGTCGGTGGTCACCGGC
CAGACCATGCTGCTGCGCGAGCAGGTGCTGAAGGAGGCCAATGTGCGATCCGGGGGCCTGCCCACCCTGGACAAGGTGAT
CCAGCGCTGCGACCGCATCGAGACGGCAACGCACCGTTGCGCGCGGATTGTCCGCAGCTTCCTCGACATGGCGCGCCAGC
GCGAGGCGGAGCGCCGGTCCACCAATCTCGCGGACGTGGCGCGTGAGGCCGTGGAACTGCTGGGCTACAACATGCGGGCC
TCGGGGGTGAAGGTGTCCCTCGACATCGCGCCCGGCCTGCCGCGCCTGATGCTGGACGCCGGGCAGATCCACCAGGTTCT
GCTCAACCTGCTGGTCAATGCCCAGCAGGCGCTGGAAGAGAAGGCCGGCGAGCGGCGCGTCGCCGTCTCGGTGGCGGTCG
ACGCGGACCGCAATGCGGTGCTGATCCGGGTCGCAGACAATGGCCCGGGCATCGCGGAGAACATCCGCTCGCGCATCTTC
GATCCGTTCTTCACCACCAAGCCCCAGGGGGTCGGCACCGGGATCGGGCTTGCCGTCTCGCGCGGGCTGATCGAGGCCCA
TGGCGGCACGCTGGAGCTGGGCACGGGCCCCGATGGCGGCGCCCTGTTCACCATCCAGCTGCCGCTGGAGGAGGTCGCCG
CCGAAGCGCCGCCAGCCGGCCCGCAGGAGCATGCGCATGACGCAGCCGGCGCGCATCTCGGATCGGTGCTGATCATCGAC
GACGAGCCGGACATTGCCGCCCTGCTCGCGGAGATCGCCGAAGGCAGCGGCTATGTGGCCGTGGTGGCGCACAGCGGCCG
GGAGGCGCAGGCGCTGCTGGCGCGGAGCGTGCCGGGCGGCCGCCCGGCCGATTTCGTCGGCATCCTGTGCGACATCCGCA
TGCCCGATGGCGATGGCCCCAGCCTCTACAACTGGCTGCTCTCCCACCGGCCCGACCTGTCGACGCGGATCGGGTTCATT
TCCGGCGACACGCTGGGGCCCTCGGCCGGGCGCTTCCTGGCCCGCTCGGGCTGCCCGCTCATCGAGAAGCCGTTCACCCC
CGCCGATGTGCGCGCCTTCCTCGGGGCTCTCGTCCGCGACGCGGCCCCGCCGCCTGAAACAACTGAAACACGGCGGACCG
AGAGCTGA

Upstream 100 bases:

>100_bases
GGCCTACTCAACCTTGTCGAAGCGAGCGTGGCACGGCTCCCGGCCCCGTCGCGCAAGTCCAAGCCGGCGGTGCCGCAAGA
CGGCGGTGCAGGAGGACGCG

Downstream 100 bases:

>100_bases
CCGCCTGCAGCAACACATGCCGGTCAAAGTACGCCCCGTTCACCGCAAGGACGGCCATGCACGACGACCAGATCGGGCTT
CTCGAAACCAGCTTCGCCGA

Product: PAS/PAC sensor hybrid histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 695; Mature: 694

Protein sequence:

>695_residues
MTSALDTQEIVPDGGGDASLVRLSDLGLFTALFQSIPGRAVLLDSSGVCRFANQEFMDFLGRSRDEVIDRPVQDFLEPSV
FESYAPLMERVFGGEQVRRVAWVDYGRLGKRYLEEVISPYRVDLTGPVVGCFGVGRDLTDMKHQEMEIVRRTQAQMEAEA
YHAAIVGTALDGIVVVDEEGSVVDFNPAAEQIFGYRKEEALGRSIAELLVPPELRAAHAAGMRSYLATGITRLIGRRLEL
PALLADGTRIPIELAITDVTRGERRLFAAHMRDLRAAKRAEEEIKRQRNALHQKEKLAALGSLLAGVAHELNNPLSVVTG
QTMLLREQVLKEANVRSGGLPTLDKVIQRCDRIETATHRCARIVRSFLDMARQREAERRSTNLADVAREAVELLGYNMRA
SGVKVSLDIAPGLPRLMLDAGQIHQVLLNLLVNAQQALEEKAGERRVAVSVAVDADRNAVLIRVADNGPGIAENIRSRIF
DPFFTTKPQGVGTGIGLAVSRGLIEAHGGTLELGTGPDGGALFTIQLPLEEVAAEAPPAGPQEHAHDAAGAHLGSVLIID
DEPDIAALLAEIAEGSGYVAVVAHSGREAQALLARSVPGGRPADFVGILCDIRMPDGDGPSLYNWLLSHRPDLSTRIGFI
SGDTLGPSAGRFLARSGCPLIEKPFTPADVRAFLGALVRDAAPPPETTETRRTES

Sequences:

>Translated_695_residues
MTSALDTQEIVPDGGGDASLVRLSDLGLFTALFQSIPGRAVLLDSSGVCRFANQEFMDFLGRSRDEVIDRPVQDFLEPSV
FESYAPLMERVFGGEQVRRVAWVDYGRLGKRYLEEVISPYRVDLTGPVVGCFGVGRDLTDMKHQEMEIVRRTQAQMEAEA
YHAAIVGTALDGIVVVDEEGSVVDFNPAAEQIFGYRKEEALGRSIAELLVPPELRAAHAAGMRSYLATGITRLIGRRLEL
PALLADGTRIPIELAITDVTRGERRLFAAHMRDLRAAKRAEEEIKRQRNALHQKEKLAALGSLLAGVAHELNNPLSVVTG
QTMLLREQVLKEANVRSGGLPTLDKVIQRCDRIETATHRCARIVRSFLDMARQREAERRSTNLADVAREAVELLGYNMRA
SGVKVSLDIAPGLPRLMLDAGQIHQVLLNLLVNAQQALEEKAGERRVAVSVAVDADRNAVLIRVADNGPGIAENIRSRIF
DPFFTTKPQGVGTGIGLAVSRGLIEAHGGTLELGTGPDGGALFTIQLPLEEVAAEAPPAGPQEHAHDAAGAHLGSVLIID
DEPDIAALLAEIAEGSGYVAVVAHSGREAQALLARSVPGGRPADFVGILCDIRMPDGDGPSLYNWLLSHRPDLSTRIGFI
SGDTLGPSAGRFLARSGCPLIEKPFTPADVRAFLGALVRDAAPPPETTETRRTES
>Mature_694_residues
TSALDTQEIVPDGGGDASLVRLSDLGLFTALFQSIPGRAVLLDSSGVCRFANQEFMDFLGRSRDEVIDRPVQDFLEPSVF
ESYAPLMERVFGGEQVRRVAWVDYGRLGKRYLEEVISPYRVDLTGPVVGCFGVGRDLTDMKHQEMEIVRRTQAQMEAEAY
HAAIVGTALDGIVVVDEEGSVVDFNPAAEQIFGYRKEEALGRSIAELLVPPELRAAHAAGMRSYLATGITRLIGRRLELP
ALLADGTRIPIELAITDVTRGERRLFAAHMRDLRAAKRAEEEIKRQRNALHQKEKLAALGSLLAGVAHELNNPLSVVTGQ
TMLLREQVLKEANVRSGGLPTLDKVIQRCDRIETATHRCARIVRSFLDMARQREAERRSTNLADVAREAVELLGYNMRAS
GVKVSLDIAPGLPRLMLDAGQIHQVLLNLLVNAQQALEEKAGERRVAVSVAVDADRNAVLIRVADNGPGIAENIRSRIFD
PFFTTKPQGVGTGIGLAVSRGLIEAHGGTLELGTGPDGGALFTIQLPLEEVAAEAPPAGPQEHAHDAAGAHLGSVLIIDD
EPDIAALLAEIAEGSGYVAVVAHSGREAQALLARSVPGGRPADFVGILCDIRMPDGDGPSLYNWLLSHRPDLSTRIGFIS
GDTLGPSAGRFLARSGCPLIEKPFTPADVRAFLGALVRDAAPPPETTETRRTES

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=386, Percent_Identity=30.5699481865285, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1790436, Length=264, Percent_Identity=31.0606060606061, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1790300, Length=381, Percent_Identity=27.5590551181102, Blast_Score=106, Evalue=6e-24,
Organism=Escherichia coli, GI87081816, Length=398, Percent_Identity=26.6331658291457, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1786912, Length=234, Percent_Identity=26.4957264957265, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790346, Length=234, Percent_Identity=27.7777777777778, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI48994928, Length=366, Percent_Identity=24.8633879781421, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI145693157, Length=270, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789808, Length=231, Percent_Identity=27.7056277056277, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788713, Length=344, Percent_Identity=25, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI87081921, Length=116, Percent_Identity=34.4827586206897, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1789149, Length=241, Percent_Identity=25.7261410788382, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 75038; Mature: 74906

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50112 PAS ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSALDTQEIVPDGGGDASLVRLSDLGLFTALFQSIPGRAVLLDSSGVCRFANQEFMDFL
CCCCCCCHHHCCCCCCCCEEEEEHHHHHHHHHHHHCCCCEEEECCCCCEECCCHHHHHHH
GRSRDEVIDRPVQDFLEPSVFESYAPLMERVFGGEQVRRVAWVDYGRLGKRYLEEVISPY
CCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCE
RVDLTGPVVGCFGVGRDLTDMKHQEMEIVRRTQAQMEAEAYHAAIVGTALDGIVVVDEEG
EEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
SVVDFNPAAEQIFGYRKEEALGRSIAELLVPPELRAAHAAGMRSYLATGITRLIGRRLEL
CEEECCHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PALLADGTRIPIELAITDVTRGERRLFAAHMRDLRAAKRAEEEIKRQRNALHQKEKLAAL
CHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSLLAGVAHELNNPLSVVTGQTMLLREQVLKEANVRSGGLPTLDKVIQRCDRIETATHRC
HHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ARIVRSFLDMARQREAERRSTNLADVAREAVELLGYNMRASGVKVSLDIAPGLPRLMLDA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEECCCEEEEEEECCCCHHHHHCH
GQIHQVLLNLLVNAQQALEEKAGERRVAVSVAVDADRNAVLIRVADNGPGIAENIRSRIF
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHC
DPFFTTKPQGVGTGIGLAVSRGLIEAHGGTLELGTGPDGGALFTIQLPLEEVAAEAPPAG
CCHHCCCCCCCCCCHHHHHHCCHHEECCCEEEECCCCCCCEEEEEECCHHHHHHCCCCCC
PQEHAHDAAGAHLGSVLIIDDEPDIAALLAEIAEGSGYVAVVAHSGREAQALLARSVPGG
CHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCC
RPADFVGILCDIRMPDGDGPSLYNWLLSHRPDLSTRIGFISGDTLGPSAGRFLARSGCPL
CCCHHEEEEEEEECCCCCCHHHHHHHHHCCCCCHHEEEEEECCCCCCCHHHHHHHCCCCC
IEKPFTPADVRAFLGALVRDAAPPPETTETRRTES
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSALDTQEIVPDGGGDASLVRLSDLGLFTALFQSIPGRAVLLDSSGVCRFANQEFMDFL
CCCCCCHHHCCCCCCCCEEEEEHHHHHHHHHHHHCCCCEEEECCCCCEECCCHHHHHHH
GRSRDEVIDRPVQDFLEPSVFESYAPLMERVFGGEQVRRVAWVDYGRLGKRYLEEVISPY
CCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCE
RVDLTGPVVGCFGVGRDLTDMKHQEMEIVRRTQAQMEAEAYHAAIVGTALDGIVVVDEEG
EEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
SVVDFNPAAEQIFGYRKEEALGRSIAELLVPPELRAAHAAGMRSYLATGITRLIGRRLEL
CEEECCHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PALLADGTRIPIELAITDVTRGERRLFAAHMRDLRAAKRAEEEIKRQRNALHQKEKLAAL
CHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSLLAGVAHELNNPLSVVTGQTMLLREQVLKEANVRSGGLPTLDKVIQRCDRIETATHRC
HHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ARIVRSFLDMARQREAERRSTNLADVAREAVELLGYNMRASGVKVSLDIAPGLPRLMLDA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEECCCEEEEEEECCCCHHHHHCH
GQIHQVLLNLLVNAQQALEEKAGERRVAVSVAVDADRNAVLIRVADNGPGIAENIRSRIF
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHC
DPFFTTKPQGVGTGIGLAVSRGLIEAHGGTLELGTGPDGGALFTIQLPLEEVAAEAPPAG
CCHHCCCCCCCCCCHHHHHHCCHHEECCCEEEECCCCCCCEEEEEECCHHHHHHCCCCCC
PQEHAHDAAGAHLGSVLIIDDEPDIAALLAEIAEGSGYVAVVAHSGREAQALLARSVPGG
CHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCC
RPADFVGILCDIRMPDGDGPSLYNWLLSHRPDLSTRIGFISGDTLGPSAGRFLARSGCPL
CCCHHEEEEEEEECCCCCCHHHHHHHHHCCCCCHHEEEEEECCCCCCCHHHHHHHCCCCC
IEKPFTPADVRAFLGALVRDAAPPPETTETRRTES
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2046550; 1766374 [H]