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The map label for this gene is def [C]

Identifier: 154244580

GI number: 154244580

Start: 706291

End: 706797

Strand: Direct

Name: def [C]

Synonym: Xaut_0628

Alternate gene names: 154244580

Gene position: 706291-706797 (Clockwise)

Preceding gene: 154244578

Following gene: 154244582

Centisome position: 13.3

GC content: 68.05

Gene sequence:

>507_bases
GTGACGGCAAGGCAGATCATCACCTTCCCGGATGCGCGCCTGCGCAGCGTCGCCGCGCCTGTGACGGTCTTCGATGATGC
GCTGGCAAATTTGGTCGCCGACCTGCTCGATACCCTGCGTGCGGCGCCGGGGATCGGGATCACGGCACCGCATATCGGCA
TCGGCAAGCGGGTGGTGGTGCTGGAGCTTTCGGCGGAGGACGGCGTGCGCGCCTATATCAATCCGCAGATCGTATGGGCG
TCGGCGGAGCTGATCCGGCATGCGGAAGGTAGCGTGTCCATGCCCGGCGTGACGGAGGAGGTGGAGCGCCCGGCGCGCGT
CCACGTGCGCTACCAGGACCTGAGCGGCGCCGAACACCTCGAGGAGGCCGATGGCCTGCGGGCGGTCTGCCACCAGCACG
AGATCGACCAGCTCGACGGCGTGTTCTGGATCCAGAGGCTCTCGCGACTGAAGCGGGAGCGCGTGATCAAGCGCTATGAG
AAGGCCCGGGGCGCGACGCGGGGCTGA

Upstream 100 bases:

>100_bases
CGAAGACGGGCTTCGCCGCCGTCGGCCGGTGGTCGAGGCACTCGGACCGTCAGGACTTCCACCGACGTGCCGGAGAAGCG
GCAGAGCGAGGCGGCGGATC

Downstream 100 bases:

>100_bases
ACCCGCGCCGATCCTTCCGGGCTTCAAGCCTCACATTCACTTGGCGAACAGCTGGCCCAGGTCTTCGAACGCCTTGATCT
CGATGGCGTTGCCGCTCGGG

Product: peptide deformylase

Products: Formate; L-Methionine. [C]

Alternate protein names: Polypeptide deformylase-like [H]

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MTARQIITFPDARLRSVAAPVTVFDDALANLVADLLDTLRAAPGIGITAPHIGIGKRVVVLELSAEDGVRAYINPQIVWA
SAELIRHAEGSVSMPGVTEEVERPARVHVRYQDLSGAEHLEEADGLRAVCHQHEIDQLDGVFWIQRLSRLKRERVIKRYE
KARGATRG

Sequences:

>Translated_168_residues
MTARQIITFPDARLRSVAAPVTVFDDALANLVADLLDTLRAAPGIGITAPHIGIGKRVVVLELSAEDGVRAYINPQIVWA
SAELIRHAEGSVSMPGVTEEVERPARVHVRYQDLSGAEHLEEADGLRAVCHQHEIDQLDGVFWIQRLSRLKRERVIKRYE
KARGATRG
>Mature_167_residues
TARQIITFPDARLRSVAAPVTVFDDALANLVADLLDTLRAAPGIGITAPHIGIGKRVVVLELSAEDGVRAYINPQIVWAS
AELIRHAEGSVSMPGVTEEVERPARVHVRYQDLSGAEHLEEADGLRAVCHQHEIDQLDGVFWIQRLSRLKRERVIKRYEK
ARGATRG

Specific function: Removes The Formyl Group From The N-Terminal Met Of Newly Synthesized Proteins. Requires At Least A Dipeptide For An Efficient Rate Of Reaction. N-Terminal L-Methionine Is A Prerequisite For Activity But The Enzyme Has Broad Specificity At Other Positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=162, Percent_Identity=36.4197530864198, Blast_Score=100, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24645728, Length=156, Percent_Identity=27.5641025641026, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: 3.5.1.88 [C]

Molecular weight: Translated: 18576; Mature: 18444

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARQIITFPDARLRSVAAPVTVFDDALANLVADLLDTLRAAPGIGITAPHIGIGKRVVV
CCCCCEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
LELSAEDGVRAYINPQIVWASAELIRHAEGSVSMPGVTEEVERPARVHVRYQDLSGAEHL
EEECCCCCCEEEECCEEEEHHHHHHHHCCCCCCCCCCHHHHCCCCEEEEEECCCCCHHHH
EEADGLRAVCHQHEIDQLDGVFWIQRLSRLKRERVIKRYEKARGATRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TARQIITFPDARLRSVAAPVTVFDDALANLVADLLDTLRAAPGIGITAPHIGIGKRVVV
CCCCEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
LELSAEDGVRAYINPQIVWASAELIRHAEGSVSMPGVTEEVERPARVHVRYQDLSGAEHL
EEECCCCCCEEEECCEEEEHHHHHHHHCCCCCCCCCCHHHHCCCCEEEEEECCCCCHHHH
EEADGLRAVCHQHEIDQLDGVFWIQRLSRLKRERVIKRYEKARGATRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Formyl-L-Methionine; H2O [C]

Specific reaction: N-Formyl-L-Methionine + H2O = Formate + L-Methionine. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: Dithiothreitol; EDTA; o-Phenantroline [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]