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The map label for this gene is rstB [H]

Identifier: 154244331

GI number: 154244331

Start: 388340

End: 389791

Strand: Direct

Name: rstB [H]

Synonym: Xaut_0374

Alternate gene names: 154244331

Gene position: 388340-389791 (Clockwise)

Preceding gene: 154244330

Following gene: 154244336

Centisome position: 7.31

GC content: 73.21

Gene sequence:

>1452_bases
ATGTCCGCACCCTCTGAAAAGCCCCCCGGCACCCGCTCCAGGGACCGCGCCTTGCCCCGTTTCGGTATCGCCGCGCGCAT
CGCGATCATTGTCGTCGGGGCGCTGGTGGTGGTGCAGGTGCTGATGCTGGTGGCCTACATCACCGAGCGCCGCGGCGCCG
CCCCCCTCGGTCCGTTCGTGCCCATGCTGCAGCGGGTGGCGGCCCTGGCCCAGCTGCTGGAGGAGGTGGGGCCGACCCAG
CGCGAGCTGGCCCTGCGCGCCACCACCGTTTCCGGATTTGCACCGGTCTACGTCACCGACCGCCCGCAGGTGGAGACGCC
GGCCTATCTCGGCTTCGCTGCGGCACGCATTCGCCAGCTCATGGGCGGACCGCCTGATCGCTTCGTGGCGCTGGCCCTCA
TCGGTGGCGAGACCGTGGGGCTCGGCCGGGTGGAGCGCCTGCGCGACCTTCGGGGGGCGCGGCTGAGGCTCGTGGTGGGG
CTCGAAAGCGGGGGCTATCTCGACGTGCTCGCGGGCGGCGACCTGACCGTTCGCCTCCTGGGCCTGCCGGTGGGGCTGAT
CGCCGGCATCCTCGGCTTCATGGTGGCGCTGGCCGCCTTGTTCGCCGTGCGGCGGGAGACCCGACCGCTCTCCGATCTCG
CCGAGGTGGTGGAGCGCTTCGGCGCGCGGCTGGAGCCGCTGGAGGTGCCCGAGCGCGGGGCGCCGGACGTGCGCCTCGTC
ATCCGCGCGGTGAACGCCATGCAGGCGCGCATCGCCGAACTGGTGCGCATGCGCACGCTGGTGCTCGGCGCCATCAGCCA
CGATCTGCGGACCTATCTCACCCGGCTGCGGCTACGGCTGGAATTGCTGCCGCCGGGGCCGCAGGTGGAGAAGGCGCAGG
CCGACCTTTCCGGGATGCAGGCGCTGGTGGACGACGCCCTCGCCTTCGCCCGCGCCACCTTCGCCCCGACCCAGGGCGAG
CGGGTGGACCTTTGCGCCCTTGCCCGCGCCGAAGTCGAGGCGCGCCTCGCCGAGGGGGGCGCTGTGAGCCTGTCCGGGGC
CGACGCGCCGCTGCTGGTGAATGGTGCGCGCGGGGCGCTGGCGCGGGTGCTGGCCAATCTCGTTGGCAACGCCCTCACTT
ATGGAACGTGCGCCGACATCGCGCTCATCGACCTCGGCGATGAGGTGGAGCTGCGCGTGGACGATCGCGGCCCCGGCATT
CCGGTGGCCGAGCGGGCGAGCGTGTTCGAGCCCTTCTACCGGGTCGAGCCCTCGCGCAACCGGGACAGCGGCGGGGCCGG
GCTCGGCCTCACCATCGTGCGGCAGATCGTGGAGGGGCACGGCGGCGAGGTCGTCATCGACGATCGTCCGGGCGGTGGCG
CGCGCCTGCGGGTGCTGCTGCCGAAGGCGGGCTCGGCCGATCAGGCGGAGAGCGCCGCCTCCACCCCCGCCGCGACGGCG
AAGAGGGACTGA

Upstream 100 bases:

>100_bases
GTCTCGGTGAGCCGGTTGAGGCGCAAGCTCGTCGCCGCTGCGCCCGAGGCGGCCCTGCTCATCAAGACCGTCCGCAACGG
CGGCTACCTTTTCGCCGCCG

Downstream 100 bases:

>100_bases
TCGGTGCCCGGCGCGCCGGCCAGCATCAGCCCCACGGGCGCGCCGGGGAACGGCAGCGAGATGGCGCAGCCGTCGATCAT
GTTGATGAGGGTGGGGTTGC

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MSAPSEKPPGTRSRDRALPRFGIAARIAIIVVGALVVVQVLMLVAYITERRGAAPLGPFVPMLQRVAALAQLLEEVGPTQ
RELALRATTVSGFAPVYVTDRPQVETPAYLGFAAARIRQLMGGPPDRFVALALIGGETVGLGRVERLRDLRGARLRLVVG
LESGGYLDVLAGGDLTVRLLGLPVGLIAGILGFMVALAALFAVRRETRPLSDLAEVVERFGARLEPLEVPERGAPDVRLV
IRAVNAMQARIAELVRMRTLVLGAISHDLRTYLTRLRLRLELLPPGPQVEKAQADLSGMQALVDDALAFARATFAPTQGE
RVDLCALARAEVEARLAEGGAVSLSGADAPLLVNGARGALARVLANLVGNALTYGTCADIALIDLGDEVELRVDDRGPGI
PVAERASVFEPFYRVEPSRNRDSGGAGLGLTIVRQIVEGHGGEVVIDDRPGGGARLRVLLPKAGSADQAESAASTPAATA
KRD

Sequences:

>Translated_483_residues
MSAPSEKPPGTRSRDRALPRFGIAARIAIIVVGALVVVQVLMLVAYITERRGAAPLGPFVPMLQRVAALAQLLEEVGPTQ
RELALRATTVSGFAPVYVTDRPQVETPAYLGFAAARIRQLMGGPPDRFVALALIGGETVGLGRVERLRDLRGARLRLVVG
LESGGYLDVLAGGDLTVRLLGLPVGLIAGILGFMVALAALFAVRRETRPLSDLAEVVERFGARLEPLEVPERGAPDVRLV
IRAVNAMQARIAELVRMRTLVLGAISHDLRTYLTRLRLRLELLPPGPQVEKAQADLSGMQALVDDALAFARATFAPTQGE
RVDLCALARAEVEARLAEGGAVSLSGADAPLLVNGARGALARVLANLVGNALTYGTCADIALIDLGDEVELRVDDRGPGI
PVAERASVFEPFYRVEPSRNRDSGGAGLGLTIVRQIVEGHGGEVVIDDRPGGGARLRVLLPKAGSADQAESAASTPAATA
KRD
>Mature_482_residues
SAPSEKPPGTRSRDRALPRFGIAARIAIIVVGALVVVQVLMLVAYITERRGAAPLGPFVPMLQRVAALAQLLEEVGPTQR
ELALRATTVSGFAPVYVTDRPQVETPAYLGFAAARIRQLMGGPPDRFVALALIGGETVGLGRVERLRDLRGARLRLVVGL
ESGGYLDVLAGGDLTVRLLGLPVGLIAGILGFMVALAALFAVRRETRPLSDLAEVVERFGARLEPLEVPERGAPDVRLVI
RAVNAMQARIAELVRMRTLVLGAISHDLRTYLTRLRLRLELLPPGPQVEKAQADLSGMQALVDDALAFARATFAPTQGER
VDLCALARAEVEARLAEGGAVSLSGADAPLLVNGARGALARVLANLVGNALTYGTCADIALIDLGDEVELRVDDRGPGIP
VAERASVFEPFYRVEPSRNRDSGGAGLGLTIVRQIVEGHGGEVVIDDRPGGGARLRVLLPKAGSADQAESAASTPAATAK
RD

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=315, Percent_Identity=32.6984126984127, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789808, Length=276, Percent_Identity=32.2463768115942, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1790346, Length=230, Percent_Identity=32.1739130434783, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1788393, Length=262, Percent_Identity=32.0610687022901, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1786600, Length=277, Percent_Identity=29.6028880866426, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1786912, Length=245, Percent_Identity=33.8775510204082, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1790551, Length=286, Percent_Identity=30.7692307692308, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789149, Length=315, Percent_Identity=25.7142857142857, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786783, Length=326, Percent_Identity=26.0736196319018, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI145693157, Length=227, Percent_Identity=26.431718061674, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87082128, Length=293, Percent_Identity=27.6450511945392, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788549, Length=244, Percent_Identity=25.8196721311475, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790861, Length=242, Percent_Identity=27.6859504132231, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50980; Mature: 50849

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPSEKPPGTRSRDRALPRFGIAARIAIIVVGALVVVQVLMLVAYITERRGAAPLGPFV
CCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
PMLQRVAALAQLLEEVGPTQRELALRATTVSGFAPVYVTDRPQVETPAYLGFAAARIRQL
HHHHHHHHHHHHHHHHCCCHHHHEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHH
MGGPPDRFVALALIGGETVGLGRVERLRDLRGARLRLVVGLESGGYLDVLAGGDLTVRLL
HCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCEEEEEE
GLPVGLIAGILGFMVALAALFAVRRETRPLSDLAEVVERFGARLEPLEVPERGAPDVRLV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
IRAVNAMQARIAELVRMRTLVLGAISHDLRTYLTRLRLRLELLPPGPQVEKAQADLSGMQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHH
ALVDDALAFARATFAPTQGERVDLCALARAEVEARLAEGGAVSLSGADAPLLVNGARGAL
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEECCHHHH
ARVLANLVGNALTYGTCADIALIDLGDEVELRVDDRGPGIPVAERASVFEPFYRVEPSRN
HHHHHHHHCCHHCCCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHCHHHHCCCCCC
RDSGGAGLGLTIVRQIVEGHGGEVVIDDRPGGGARLRVLLPKAGSADQAESAASTPAATA
CCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCCCHHHHHCCCCCCCC
KRD
CCC
>Mature Secondary Structure 
SAPSEKPPGTRSRDRALPRFGIAARIAIIVVGALVVVQVLMLVAYITERRGAAPLGPFV
CCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
PMLQRVAALAQLLEEVGPTQRELALRATTVSGFAPVYVTDRPQVETPAYLGFAAARIRQL
HHHHHHHHHHHHHHHHCCCHHHHEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHH
MGGPPDRFVALALIGGETVGLGRVERLRDLRGARLRLVVGLESGGYLDVLAGGDLTVRLL
HCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCEEEEEE
GLPVGLIAGILGFMVALAALFAVRRETRPLSDLAEVVERFGARLEPLEVPERGAPDVRLV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
IRAVNAMQARIAELVRMRTLVLGAISHDLRTYLTRLRLRLELLPPGPQVEKAQADLSGMQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHH
ALVDDALAFARATFAPTQGERVDLCALARAEVEARLAEGGAVSLSGADAPLLVNGARGAL
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEECCHHHH
ARVLANLVGNALTYGTCADIALIDLGDEVELRVDDRGPGIPVAERASVFEPFYRVEPSRN
HHHHHHHHCCHHCCCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHCHHHHCCCCCC
RDSGGAGLGLTIVRQIVEGHGGEVVIDDRPGGGARLRVLLPKAGSADQAESAASTPAATA
CCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCCCHHHHHCCCCCCCC
KRD
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]