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The map label for this gene is pncA [H]

Identifier: 154244306

GI number: 154244306

Start: 363003

End: 363749

Strand: Reverse

Name: pncA [H]

Synonym: Xaut_0349

Alternate gene names: 154244306

Gene position: 363749-363003 (Counterclockwise)

Preceding gene: 154244310

Following gene: 154244303

Centisome position: 6.85

GC content: 69.61

Gene sequence:

>747_bases
TTGGCGCCGCAAGGCGTGCCGGCTCGCGGTCTGTTCCTTTTTCACGTCCGCGCGAGCCTGCGCGTCATCGCAAAGGACCA
TACGATGCCCGCCCCCTCCCCGGCCCCGCTCGCAACCGCCGGCAACAGGACCCGCTCCGCGCTCGTCGCGGTGGACCTGC
AGGTGGACTTCCTGCCCGGCGGCCGGCTGGCGGTACCGGGCGGGCATGAGGTGGTGCCGCTGGCAAACCTCGCGGCGCGG
CATTTCCCCCATGTGGTGCTGACACAGGACTGGCACCCGCCGGGCCATGTCTCGTTCGCCTCGAGCCACCCGGGCAAGGC
GCCCTTCGACGAGATCGACCTGCCCTATGGGCGACAGATCCTGTGGCCGGACCATTGCGTGCAGGACAGCGAGGGCGCCC
GCCTGTCCGCCGAACTCGACGTTCCCCACGCCCAAGTGGTCATCCGCAAGGGGCACCACCGCACCATCGACAGCTATTCC
ACCTTCTACGAGGCGGACCGCACCACTCCCACCGGCCTCTCCGGATACCTGCGGGACCGGGGTATCGATACGGTGTATCT
GCTCGGGCTGGCGACGGACTTCTGCGTGTCCTGGTCGGCGGTGGACGCCGCCCGGCACGGGTTCGTCACCCATGTGATCG
AGGATGCCTGCCGGGCGCTGGACGTGGACGGATCCCTGGCCAAGGCCTACGCCGACATGGCCTCCGCCGGGGTGCAACGG
GTGCGGCTGGCCGATGTGATCGCCTGA

Upstream 100 bases:

>100_bases
GGCCGCAGGCCCCTCCCGCAACCGATCCGCCGGCCCCCTGAACTTTCGAATTGGGGTTCGTCGCGACAACGACGATCGAG
GCGGATATAGGGGGGGTGTT

Downstream 100 bases:

>100_bases
CGCACGGTGACCTGCGCCGCCCCCGGCTCATGCCAACGGCCGATAAGCCAGGCTCATCGTCCATCGGCGAGCCCGCGCTG
CACATGAGAGAACGCGCCTC

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MAPQGVPARGLFLFHVRASLRVIAKDHTMPAPSPAPLATAGNRTRSALVAVDLQVDFLPGGRLAVPGGHEVVPLANLAAR
HFPHVVLTQDWHPPGHVSFASSHPGKAPFDEIDLPYGRQILWPDHCVQDSEGARLSAELDVPHAQVVIRKGHHRTIDSYS
TFYEADRTTPTGLSGYLRDRGIDTVYLLGLATDFCVSWSAVDAARHGFVTHVIEDACRALDVDGSLAKAYADMASAGVQR
VRLADVIA

Sequences:

>Translated_248_residues
MAPQGVPARGLFLFHVRASLRVIAKDHTMPAPSPAPLATAGNRTRSALVAVDLQVDFLPGGRLAVPGGHEVVPLANLAAR
HFPHVVLTQDWHPPGHVSFASSHPGKAPFDEIDLPYGRQILWPDHCVQDSEGARLSAELDVPHAQVVIRKGHHRTIDSYS
TFYEADRTTPTGLSGYLRDRGIDTVYLLGLATDFCVSWSAVDAARHGFVTHVIEDACRALDVDGSLAKAYADMASAGVQR
VRLADVIA
>Mature_247_residues
APQGVPARGLFLFHVRASLRVIAKDHTMPAPSPAPLATAGNRTRSALVAVDLQVDFLPGGRLAVPGGHEVVPLANLAARH
FPHVVLTQDWHPPGHVSFASSHPGKAPFDEIDLPYGRQILWPDHCVQDSEGARLSAELDVPHAQVVIRKGHHRTIDSYST
FYEADRTTPTGLSGYLRDRGIDTVYLLGLATDFCVSWSAVDAARHGFVTHVIEDACRALDVDGSLAKAYADMASAGVQRV
RLADVIA

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=204, Percent_Identity=42.156862745098, Blast_Score=150, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI193206735, Length=203, Percent_Identity=33.4975369458128, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17543130, Length=203, Percent_Identity=33.4975369458128, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71999339, Length=204, Percent_Identity=31.8627450980392, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321401, Length=197, Percent_Identity=34.5177664974619, Blast_Score=108, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24648247, Length=204, Percent_Identity=35.7843137254902, Blast_Score=113, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 26713; Mature: 26581

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPQGVPARGLFLFHVRASLRVIAKDHTMPAPSPAPLATAGNRTRSALVAVDLQVDFLPG
CCCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCC
GRLAVPGGHEVVPLANLAARHFPHVVLTQDWHPPGHVSFASSHPGKAPFDEIDLPYGRQI
CEEECCCCCCEEEHHHHHHHHCCEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCCEE
LWPDHCVQDSEGARLSAELDVPHAQVVIRKGHHRTIDSYSTFYEADRTTPTGLSGYLRDR
ECCHHHCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHHC
GIDTVYLLGLATDFCVSWSAVDAARHGFVTHVIEDACRALDVDGSLAKAYADMASAGVQR
CCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VRLADVIA
HHHHHHCC
>Mature Secondary Structure 
APQGVPARGLFLFHVRASLRVIAKDHTMPAPSPAPLATAGNRTRSALVAVDLQVDFLPG
CCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCC
GRLAVPGGHEVVPLANLAARHFPHVVLTQDWHPPGHVSFASSHPGKAPFDEIDLPYGRQI
CEEECCCCCCEEEHHHHHHHHCCEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCCEE
LWPDHCVQDSEGARLSAELDVPHAQVVIRKGHHRTIDSYSTFYEADRTTPTGLSGYLRDR
ECCHHHCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHHC
GIDTVYLLGLATDFCVSWSAVDAARHGFVTHVIEDACRALDVDGSLAKAYADMASAGVQR
CCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VRLADVIA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]