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The map label for this gene is alkB [H]

Identifier: 154244303

GI number: 154244303

Start: 360942

End: 361598

Strand: Reverse

Name: alkB [H]

Synonym: Xaut_0346

Alternate gene names: 154244303

Gene position: 361598-360942 (Counterclockwise)

Preceding gene: 154244306

Following gene: 154244302

Centisome position: 6.81

GC content: 70.32

Gene sequence:

>657_bases
ATGACGGCAACGCCCGCTTCCGCGCCGGCCCAAATGCCAGCCCATCTGGAGGTGGCCCCCGGCGCCCTGTGGTTTCGCGA
GGCGCTGGACCGGGACGCGCAGGCCGCCCTGCTTGCGGACATCCGCGCGGTGCTGGACAAAGCCCCGCTGTTCACGCCGG
CCATGCCGCGGACCGGAAAGCCGTTTTCCGTCCGCATGAGCAATTGCGGGCCGCTCGGCTGGGTCTCGGACGCTGCGGGA
TATCGCTACCAACCGTTCCATCCCGAGACCCGCGCGCCCTGGCCCGCCATGCCGCCGGCGCTGGCCGCGCTGTGGACCAC
CTATGCGGCCTTCAAGGCCCCGGCCGAGGCCTGCCTCATCAATTATTACGGGCCGGATGCGCGCATGAGCCTGCATCAGG
ACCGCGACGAAGCCGACTTCACCGCGCCTGTCCTCTCCCTCTCGCTGGGGGACACCGCCCTGTTCCGCATCGGCGGTGAG
ACGCGGGGCGGCGCCACCCGTTCGATCCGCCTCTCCTCCGGCGACATCTTCCTCTTGTCCGGACCGAGCCGGCTCGCGTT
CCACGGCATCGACCGCATCCTGCCCGGCACCTCCACGCTGCTGCCCGAGGGCGGACGCTTCAACCTCACGCTGCGGCGCG
TCAATCCAGCTACCTGA

Upstream 100 bases:

>100_bases
TATGGTTACTTCCCCATTAAAGCCATGGTCACGCCAAGGGAAAGCTGCCATCGAGTACCTCTGTTTTCTTTGAAATGCCG
GTTTCGGAGCGCCTGAAGAC

Downstream 100 bases:

>100_bases
AGCATCGCACCTGTGGCAACGCCCCACAGAAAAGCCCGAATGTCGCCCGAAACAGGTCCTTCGATCCGGTACCAGGGAGC
GGAGGCCCTGCCGCCGGGTC

Product: 2OG-Fe(II) oxygenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTATPASAPAQMPAHLEVAPGALWFREALDRDAQAALLADIRAVLDKAPLFTPAMPRTGKPFSVRMSNCGPLGWVSDAAG
YRYQPFHPETRAPWPAMPPALAALWTTYAAFKAPAEACLINYYGPDARMSLHQDRDEADFTAPVLSLSLGDTALFRIGGE
TRGGATRSIRLSSGDIFLLSGPSRLAFHGIDRILPGTSTLLPEGGRFNLTLRRVNPAT

Sequences:

>Translated_218_residues
MTATPASAPAQMPAHLEVAPGALWFREALDRDAQAALLADIRAVLDKAPLFTPAMPRTGKPFSVRMSNCGPLGWVSDAAG
YRYQPFHPETRAPWPAMPPALAALWTTYAAFKAPAEACLINYYGPDARMSLHQDRDEADFTAPVLSLSLGDTALFRIGGE
TRGGATRSIRLSSGDIFLLSGPSRLAFHGIDRILPGTSTLLPEGGRFNLTLRRVNPAT
>Mature_217_residues
TATPASAPAQMPAHLEVAPGALWFREALDRDAQAALLADIRAVLDKAPLFTPAMPRTGKPFSVRMSNCGPLGWVSDAAGY
RYQPFHPETRAPWPAMPPALAALWTTYAAFKAPAEACLINYYGPDARMSLHQDRDEADFTAPVLSLSLGDTALFRIGGET
RGGATRSIRLSSGDIFLLSGPSRLAFHGIDRILPGTSTLLPEGGRFNLTLRRVNPAT

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=138, Percent_Identity=35.5072463768116, Blast_Score=78, Evalue=5e-15,
Organism=Escherichia coli, GI1788541, Length=202, Percent_Identity=40.0990099009901, Blast_Score=136, Evalue=1e-33,
Organism=Drosophila melanogaster, GI45555401, Length=112, Percent_Identity=37.5, Blast_Score=77, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23430; Mature: 23299

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATPASAPAQMPAHLEVAPGALWFREALDRDAQAALLADIRAVLDKAPLFTPAMPRTGK
CCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PFSVRMSNCGPLGWVSDAAGYRYQPFHPETRAPWPAMPPALAALWTTYAAFKAPAEACLI
CEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
NYYGPDARMSLHQDRDEADFTAPVLSLSLGDTALFRIGGETRGGATRSIRLSSGDIFLLS
EEECCCCCCHHCCCCCCCCHHCCEEEEECCCEEEEEECCCCCCCCEEEEEECCCCEEEEE
GPSRLAFHGIDRILPGTSTLLPEGGRFNLTLRRVNPAT
CCCCHHHHHHHHHCCCCHHCCCCCCEEEEEEEECCCCC
>Mature Secondary Structure 
TATPASAPAQMPAHLEVAPGALWFREALDRDAQAALLADIRAVLDKAPLFTPAMPRTGK
CCCCCCCCCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PFSVRMSNCGPLGWVSDAAGYRYQPFHPETRAPWPAMPPALAALWTTYAAFKAPAEACLI
CEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
NYYGPDARMSLHQDRDEADFTAPVLSLSLGDTALFRIGGETRGGATRSIRLSSGDIFLLS
EEECCCCCCHHCCCCCCCCHHCCEEEEECCCEEEEEECCCCCCCCEEEEEECCCCEEEEE
GPSRLAFHGIDRILPGTSTLLPEGGRFNLTLRRVNPAT
CCCCHHHHHHHHHCCCCHHCCCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9150207 [H]