Click here to switch to the map view.

The map label for this gene is ddl

Identifier: 154244282

GI number: 154244282

Start: 335585

End: 336505

Strand: Direct

Name: ddl

Synonym: Xaut_0325

Alternate gene names: 154244282

Gene position: 335585-336505 (Clockwise)

Preceding gene: 154244281

Following gene: 154244283

Centisome position: 6.32

GC content: 64.93

Gene sequence:

>921_bases
ATGGCGAAACACGTGGCGGTGCTCATGGGCGGCTGGTCGTCGGAGCGCGAAGTCTCCCTGCGCTCCGGCGCCGCCTGCGC
CGGTGCGCTGGAGGCGGCAGGCTACCGGGTCACCCGCGTGGACGTGGGCCGCGACGTGGCCGAGGTGCTGACGCACCTGA
AGCCGGACGTGGCGTTCAACGTGCTGCACGGCCAGCCCGGCGAGGACGGCACCATCCAGGGCATCCTCGAAATCCTGCGC
ATCCCCTATTCCCATTCCGGCGTACTGGCCTCGGCCCTCGCCATGGACAAGGCGCAGGCGCGAATCATGCTCGCCGCCGC
TGGTGTCCCGGTGGCGAAAGGCGGCCTCGTCTCCCGCGCCGAGGCGGCAAAGGCGCACATCATGCCCACGCCCTACGTGC
TGAAGCCCAATGCGGGAGGGTCCAGCGTCGGCGTCTTCATCGTGCGCGAAGACCAGGCCCATCCGCCGCAGGAATTGACC
CGTGAGGATTGGCCGCACGGAGAAAATCTTCTCGCCGAAGAATTTATTCCCGGCCTCGAACTCACCTGCGCGGTGATGGG
CGACAAGGTGCTCGACGTGATCGAGATCGAGTCCACACAGAAGTTTTACGATTACGAAAGCAAATATGCACCGGGCGGCT
CGCAGCACATTCTTCCGGCCCGGATTTTACCCGAAATTTACCAACGGGTGCAGATGTTAAGCCTCACGGCGCACCGAGCT
CTCGGATGCCGGGGGGTGTCGCGGAGCGACTTTCGGTTCGACCCTTCGAGAGGGGATGGCTCCGGCCTGATCTGCCTCGA
GGTGAACACCCAGCCCGGGATGACTGAGACGTCTCTGGTGCCGGAACTGGCCGCCCACGCGGGCATCTCATTCGGTGAGC
TTGTGACATGGATGGTGGAGGACGCTTCGCTGGAGCGCTAA

Upstream 100 bases:

>100_bases
GCAAGGCAATGGCGCCATCTTGAGGCGCCATTTGGGGCACTGGGCAGGAGCGTCATCGGGCGCGCGGCCGAGCCGTGGAT
AGAGACCGGGAATTCAGATC

Downstream 100 bases:

>100_bases
GACCCCGCCCCGAGGCGGGGCGGAAACCGCCGCAGCGTCCGGTTCCCCTGCCGCCGCCGGGCCGGCACAAGCGCCTGCCG
GTGGAACCGCGCCCCACCAG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MAKHVAVLMGGWSSEREVSLRSGAACAGALEAAGYRVTRVDVGRDVAEVLTHLKPDVAFNVLHGQPGEDGTIQGILEILR
IPYSHSGVLASALAMDKAQARIMLAAAGVPVAKGGLVSRAEAAKAHIMPTPYVLKPNAGGSSVGVFIVREDQAHPPQELT
REDWPHGENLLAEEFIPGLELTCAVMGDKVLDVIEIESTQKFYDYESKYAPGGSQHILPARILPEIYQRVQMLSLTAHRA
LGCRGVSRSDFRFDPSRGDGSGLICLEVNTQPGMTETSLVPELAAHAGISFGELVTWMVEDASLER

Sequences:

>Translated_306_residues
MAKHVAVLMGGWSSEREVSLRSGAACAGALEAAGYRVTRVDVGRDVAEVLTHLKPDVAFNVLHGQPGEDGTIQGILEILR
IPYSHSGVLASALAMDKAQARIMLAAAGVPVAKGGLVSRAEAAKAHIMPTPYVLKPNAGGSSVGVFIVREDQAHPPQELT
REDWPHGENLLAEEFIPGLELTCAVMGDKVLDVIEIESTQKFYDYESKYAPGGSQHILPARILPEIYQRVQMLSLTAHRA
LGCRGVSRSDFRFDPSRGDGSGLICLEVNTQPGMTETSLVPELAAHAGISFGELVTWMVEDASLER
>Mature_305_residues
AKHVAVLMGGWSSEREVSLRSGAACAGALEAAGYRVTRVDVGRDVAEVLTHLKPDVAFNVLHGQPGEDGTIQGILEILRI
PYSHSGVLASALAMDKAQARIMLAAAGVPVAKGGLVSRAEAAKAHIMPTPYVLKPNAGGSSVGVFIVREDQAHPPQELTR
EDWPHGENLLAEEFIPGLELTCAVMGDKVLDVIEIESTQKFYDYESKYAPGGSQHILPARILPEIYQRVQMLSLTAHRAL
GCRGVSRSDFRFDPSRGDGSGLICLEVNTQPGMTETSLVPELAAHAGISFGELVTWMVEDASLER

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=311, Percent_Identity=39.871382636656, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI1786579, Length=356, Percent_Identity=30.0561797752809, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_XANP2 (A7IC40)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001415240.1
- ProteinModelPortal:   A7IC40
- SMR:   A7IC40
- STRING:   A7IC40
- GeneID:   5424689
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_0325
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   PLSIVPE
- ProtClustDB:   PRK01372
- BioCyc:   XAUT78245:XAUT_0325-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 32788; Mature: 32657

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHVAVLMGGWSSEREVSLRSGAACAGALEAAGYRVTRVDVGRDVAEVLTHLKPDVAFN
CCCEEEEEEECCCCCCEEEHHCCCCHHHHHHHCCEEEEEEECCCHHHHHHHHCCCCCEEE
VLHGQPGEDGTIQGILEILRIPYSHSGVLASALAMDKAQARIMLAAAGVPVAKGGLVSRA
EECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEEEEECCCCEECCCCCHHH
EAAKAHIMPTPYVLKPNAGGSSVGVFIVREDQAHPPQELTREDWPHGENLLAEEFIPGLE
HHHHHCCCCCCEEECCCCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCHHHHHCCCCCE
LTCAVMGDKVLDVIEIESTQKFYDYESKYAPGGSQHILPARILPEIYQRVQMLSLTAHRA
EEEEECCCHHEEEEEECCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LGCRGVSRSDFRFDPSRGDGSGLICLEVNTQPGMTETSLVPELAAHAGISFGELVTWMVE
HCCCCCCCCCCEECCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
DASLER
CCCCCC
>Mature Secondary Structure 
AKHVAVLMGGWSSEREVSLRSGAACAGALEAAGYRVTRVDVGRDVAEVLTHLKPDVAFN
CCEEEEEEECCCCCCEEEHHCCCCHHHHHHHCCEEEEEEECCCHHHHHHHHCCCCCEEE
VLHGQPGEDGTIQGILEILRIPYSHSGVLASALAMDKAQARIMLAAAGVPVAKGGLVSRA
EECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEEEEECCCCEECCCCCHHH
EAAKAHIMPTPYVLKPNAGGSSVGVFIVREDQAHPPQELTREDWPHGENLLAEEFIPGLE
HHHHHCCCCCCEEECCCCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCHHHHHCCCCCE
LTCAVMGDKVLDVIEIESTQKFYDYESKYAPGGSQHILPARILPEIYQRVQMLSLTAHRA
EEEEECCCHHEEEEEECCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LGCRGVSRSDFRFDPSRGDGSGLICLEVNTQPGMTETSLVPELAAHAGISFGELVTWMVE
HCCCCCCCCCCEECCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
DASLER
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA