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The map label for this gene is est [H]

Identifier: 154244278

GI number: 154244278

Start: 331758

End: 332588

Strand: Reverse

Name: est [H]

Synonym: Xaut_0321

Alternate gene names: 154244278

Gene position: 332588-331758 (Counterclockwise)

Preceding gene: 154244280

Following gene: 154244277

Centisome position: 6.26

GC content: 72.08

Gene sequence:

>831_bases
ATGGCCCCCCACTGGCAAGAGACATCCCTGCAGACGCAAGCCGGCCCCATGCCGGCGCGGCTGTATGGTGTCCACCCCAG
CAAGGGCGCAGTGCCACTCATTTTACACCTGCACGGCGGCGCTTTCACCGGCGGCACGCTGGAGTGCGGCGCGTATGTCG
CCGCCCTGCTGGCGGAGGCCGGTGCTGTGGTGGTCTCGGCCGATTATCCGCTGGCCTGCGAGCGTCCGTTCCCCTTCGCC
CTGACCGCCGCCTTCGGCGCCCTGGCTGCGCTCTACGGCCGCCGGGCCGAGTTCGCCGGCCGCAACGCCCCGCTCTACGT
GGCCGGGGAGGAATCGGGTGGCAACCTTGCCGCCGGCCTCGCCATGATGGCCCGTGACCAGCAAGCGCCGCCCCTCGCCG
GCCAGATCCTCATCTCGCCCATGCTCGACCCCAGCCTCGCCACCGCCTCGCTGCGCGCCGCCGAAGCCGGCACCTGCGGC
TGCAAGTGGGCTGACGGCTGGCAGACCTATCTGGGCTCCCCCGAGAAGGCCGCCCACCCCTATGCCGCGCCCCTCGCCTC
CTCTCGCCTGCAGGGCCTGCCGCCAGCCCTGGTGGTGAGCGCGCAGGACTGCCCCATGCGCGACGAGAGCGCGCGCTATG
CCTGCGCGCTGGAGAAGGCCGGTGTCGCCACCGAGCTTCACATCCTGCCCGGCCCGACCCACTGGCCCGAGGGCATTTCG
GCACCGCCGGAAAAGCCCGAATCCTGGGCCGGCACCATGCGCACCCTGTTCTCCCGCTTCTTCGAAACGTCCCGACCGCG
TCGGCGCAAGCCGGCCCCGGCCGTCCTCTGA

Upstream 100 bases:

>100_bases
ATGATACCATATTTCGCGGCTTTATCGGTATAATCTAGATCAATATCGTTCTCGCACTGCACAAGGCGGACGGTCCCGCC
GGCATCGAGGACGGTTCGCG

Downstream 100 bases:

>100_bases
CCGCCGAACATCCAGAACTCCCCCAGGATCTGCCCCATGACCGCCCCGATCAGCACCGCACCCCGCACCGCCCGCCTGCG
CAACCGCCTCCTCCTCGGCA

Product: alpha/beta hydrolase domain-containing protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MAPHWQETSLQTQAGPMPARLYGVHPSKGAVPLILHLHGGAFTGGTLECGAYVAALLAEAGAVVVSADYPLACERPFPFA
LTAAFGALAALYGRRAEFAGRNAPLYVAGEESGGNLAAGLAMMARDQQAPPLAGQILISPMLDPSLATASLRAAEAGTCG
CKWADGWQTYLGSPEKAAHPYAAPLASSRLQGLPPALVVSAQDCPMRDESARYACALEKAGVATELHILPGPTHWPEGIS
APPEKPESWAGTMRTLFSRFFETSRPRRRKPAPAVL

Sequences:

>Translated_276_residues
MAPHWQETSLQTQAGPMPARLYGVHPSKGAVPLILHLHGGAFTGGTLECGAYVAALLAEAGAVVVSADYPLACERPFPFA
LTAAFGALAALYGRRAEFAGRNAPLYVAGEESGGNLAAGLAMMARDQQAPPLAGQILISPMLDPSLATASLRAAEAGTCG
CKWADGWQTYLGSPEKAAHPYAAPLASSRLQGLPPALVVSAQDCPMRDESARYACALEKAGVATELHILPGPTHWPEGIS
APPEKPESWAGTMRTLFSRFFETSRPRRRKPAPAVL
>Mature_275_residues
APHWQETSLQTQAGPMPARLYGVHPSKGAVPLILHLHGGAFTGGTLECGAYVAALLAEAGAVVVSADYPLACERPFPFAL
TAAFGALAALYGRRAEFAGRNAPLYVAGEESGGNLAAGLAMMARDQQAPPLAGQILISPMLDPSLATASLRAAEAGTCGC
KWADGWQTYLGSPEKAAHPYAAPLASSRLQGLPPALVVSAQDCPMRDESARYACALEKAGVATELHILPGPTHWPEGISA
PPEKPESWAGTMRTLFSRFFETSRPRRRKPAPAVL

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 28921; Mature: 28790

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPHWQETSLQTQAGPMPARLYGVHPSKGAVPLILHLHGGAFTGGTLECGAYVAALLAEA
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHC
GAVVVSADYPLACERPFPFALTAAFGALAALYGRRAEFAGRNAPLYVAGEESGGNLAAGL
CEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHH
AMMARDQQAPPLAGQILISPMLDPSLATASLRAAEAGTCGCKWADGWQTYLGSPEKAAHP
HHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCHHHCCC
YAAPLASSRLQGLPPALVVSAQDCPMRDESARYACALEKAGVATELHILPGPTHWPEGIS
CHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEHHHCCCEEEEEECCCCCCCCCCCC
APPEKPESWAGTMRTLFSRFFETSRPRRRKPAPAVL
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
APHWQETSLQTQAGPMPARLYGVHPSKGAVPLILHLHGGAFTGGTLECGAYVAALLAEA
CCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHC
GAVVVSADYPLACERPFPFALTAAFGALAALYGRRAEFAGRNAPLYVAGEESGGNLAAGL
CEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHH
AMMARDQQAPPLAGQILISPMLDPSLATASLRAAEAGTCGCKWADGWQTYLGSPEKAAHP
HHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCHHHCCC
YAAPLASSRLQGLPPALVVSAQDCPMRDESARYACALEKAGVATELHILPGPTHWPEGIS
CHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEHHHCCCEEEEEECCCCCCCCCCCC
APPEKPESWAGTMRTLFSRFFETSRPRRRKPAPAVL
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]