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The map label for this gene is truB [H]

Identifier: 154244248

GI number: 154244248

Start: 296547

End: 297638

Strand: Reverse

Name: truB [H]

Synonym: Xaut_0291

Alternate gene names: 154244248

Gene position: 297638-296547 (Counterclockwise)

Preceding gene: 154244249

Following gene: 154244247

Centisome position: 5.61

GC content: 73.17

Gene sequence:

>1092_bases
ATGACCGCCCCCTTTGCTGCCGACGCCGCCGCCCCGATCCTCGCCGCCGAGGCTGACGCCCCCGTCGCGGCCGATGCGAC
CGCCGCTCAGGCCCTGCCGGACGGCGCCGAAGCCCCCCGCGCTTCCCGCGAGCAGGACAACCGCCCGCGCGCGCCCAAGC
GCGACCTGGACGGCTGGGTGCTGCTGGACAAGCCCACGGGCATGACCTCCACCCAGGCGGTGGCGGTGGTGAAGCGCATC
TTCGGCGCCCGCAAGGCCGGCCACGCCGGCACCCTGGACCCGCTGGCCTCCGGCTGCCTGCCCATCGCCTTCGGCGAGGC
CACCAAGACCGTCCCTTACGTGATGGACGGCCGCAAGACCTACCGCTTCACGGTGCGCTTCGGCGTCGAGACCGACACCG
ACGACAGCGAGGGCAAGGCCGTGGTCACCTCCGACCATCGCCCCACCGACGCGGACATCGTCGCCGCGCTCGCCGCCTTC
CGGGGCGAGATCATGCAGGTGCCGCCGGCCTATTCGGCCCTCAAGATCGGCGGCGAGCGCGCCTATGACCTCGCCCGCGA
GGGCGAGGAGGTGGTGCTCCAGCCCCGCCCGGTCACCATCTTCCGCATCGAGCTGGTGGAGCGGCCCGACGCCGACCATG
CGGTGCTGGAAGCCGAATGCGGCAAGGGTACCTATGTGCGCGCCATCGCCCGCGACCTCGGCCGCATGCTGGACGCGCGC
GGCCACGTCTCGGCCCTGCGCCGGGCCTGCGTCGGGCCGTTCCTGGAAGAGGACCTGGTGCCCCTCGACGAGATGCGCGA
GCGCGCCGAGGCCGGCGAGGAAGCCCTGATGGACGCCCTCGACCCGGTGGCGCTGGCGCTGGAGGAGATCCCCCAGATCG
CCGTCACCCCACCCGACGCCCATCGCCTGCGCTGCGGCCAGAGCGTGATCCTGCGCGGGCGCGACGCGCCCATCGTGCAG
GGCCATGCGGCCATTACCTGCCAGGGCGCCCTCATCGCCATCGGCGACGTGGACGCAGGCGAGATCTTCCCCCACCGCGT
CTTCAACTGGGGCCAGAAGGACAAGAAGCCCCCGCGCCGCGGCAAGCGCTGA

Upstream 100 bases:

>100_bases
TGCTGCGCTCGCCCAAGGTGGTGCGCGACCTCGATGACACCTCCTCGGATGACACGTCCCCGGACGCCAACACCGACACC
GACAAAGAGACCGACGCCGA

Downstream 100 bases:

>100_bases
AGCCTGTTTGGCAATTCAAAAAGGGGCATCCCTCACCCCTGTCATGCCCGGCCTTGTGCCGGGCATCCACGCGTGCCGCT
GGTCGCTCCGTTCGGAGCCT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTAPFAADAAAPILAAEADAPVAADATAAQALPDGAEAPRASREQDNRPRAPKRDLDGWVLLDKPTGMTSTQAVAVVKRI
FGARKAGHAGTLDPLASGCLPIAFGEATKTVPYVMDGRKTYRFTVRFGVETDTDDSEGKAVVTSDHRPTDADIVAALAAF
RGEIMQVPPAYSALKIGGERAYDLAREGEEVVLQPRPVTIFRIELVERPDADHAVLEAECGKGTYVRAIARDLGRMLDAR
GHVSALRRACVGPFLEEDLVPLDEMRERAEAGEEALMDALDPVALALEEIPQIAVTPPDAHRLRCGQSVILRGRDAPIVQ
GHAAITCQGALIAIGDVDAGEIFPHRVFNWGQKDKKPPRRGKR

Sequences:

>Translated_363_residues
MTAPFAADAAAPILAAEADAPVAADATAAQALPDGAEAPRASREQDNRPRAPKRDLDGWVLLDKPTGMTSTQAVAVVKRI
FGARKAGHAGTLDPLASGCLPIAFGEATKTVPYVMDGRKTYRFTVRFGVETDTDDSEGKAVVTSDHRPTDADIVAALAAF
RGEIMQVPPAYSALKIGGERAYDLAREGEEVVLQPRPVTIFRIELVERPDADHAVLEAECGKGTYVRAIARDLGRMLDAR
GHVSALRRACVGPFLEEDLVPLDEMRERAEAGEEALMDALDPVALALEEIPQIAVTPPDAHRLRCGQSVILRGRDAPIVQ
GHAAITCQGALIAIGDVDAGEIFPHRVFNWGQKDKKPPRRGKR
>Mature_362_residues
TAPFAADAAAPILAAEADAPVAADATAAQALPDGAEAPRASREQDNRPRAPKRDLDGWVLLDKPTGMTSTQAVAVVKRIF
GARKAGHAGTLDPLASGCLPIAFGEATKTVPYVMDGRKTYRFTVRFGVETDTDDSEGKAVVTSDHRPTDADIVAALAAFR
GEIMQVPPAYSALKIGGERAYDLAREGEEVVLQPRPVTIFRIELVERPDADHAVLEAECGKGTYVRAIARDLGRMLDARG
HVSALRRACVGPFLEEDLVPLDEMRERAEAGEEALMDALDPVALALEEIPQIAVTPPDAHRLRCGQSVILRGRDAPIVQG
HAAITCQGALIAIGDVDAGEIFPHRVFNWGQKDKKPPRRGKR

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=219, Percent_Identity=37.8995433789954, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI215599015, Length=204, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4503337, Length=204, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI2367200, Length=314, Percent_Identity=39.171974522293, Blast_Score=202, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17553978, Length=220, Percent_Identity=28.6363636363636, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323204, Length=212, Percent_Identity=31.1320754716981, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324037, Length=139, Percent_Identity=35.9712230215827, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI281364189, Length=204, Percent_Identity=28.4313725490196, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281364187, Length=204, Percent_Identity=28.4313725490196, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281364185, Length=204, Percent_Identity=28.4313725490196, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281364183, Length=204, Percent_Identity=28.4313725490196, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI62471759, Length=204, Percent_Identity=28.4313725490196, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI17975520, Length=204, Percent_Identity=28.4313725490196, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 38896; Mature: 38764

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPFAADAAAPILAAEADAPVAADATAAQALPDGAEAPRASREQDNRPRAPKRDLDGWV
CCCCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
LLDKPTGMTSTQAVAVVKRIFGARKAGHAGTLDPLASGCLPIAFGEATKTVPYVMDGRKT
EEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCEEEEECCCCCCCCEEECCCEE
YRFTVRFGVETDTDDSEGKAVVTSDHRPTDADIVAALAAFRGEIMQVPPAYSALKIGGER
EEEEEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCEEECCCCHHHHHCCCHH
AYDLAREGEEVVLQPRPVTIFRIELVERPDADHAVLEAECGKGTYVRAIARDLGRMLDAR
HHHHHHCCCEEEECCCCEEEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCC
GHVSALRRACVGPFLEEDLVPLDEMRERAEAGEEALMDALDPVALALEEIPQIAVTPPDA
HHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCH
HRLRCGQSVILRGRDAPIVQGHAAITCQGALIAIGDVDAGEIFPHRVFNWGQKDKKPPRR
HHHHCCCEEEEECCCCCEEECCEEEEECCEEEEEECCCCCCCCHHHHHCCCCCCCCCCCC
GKR
CCC
>Mature Secondary Structure 
TAPFAADAAAPILAAEADAPVAADATAAQALPDGAEAPRASREQDNRPRAPKRDLDGWV
CCCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
LLDKPTGMTSTQAVAVVKRIFGARKAGHAGTLDPLASGCLPIAFGEATKTVPYVMDGRKT
EEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCEEEEECCCCCCCCEEECCCEE
YRFTVRFGVETDTDDSEGKAVVTSDHRPTDADIVAALAAFRGEIMQVPPAYSALKIGGER
EEEEEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCEEECCCCHHHHHCCCHH
AYDLAREGEEVVLQPRPVTIFRIELVERPDADHAVLEAECGKGTYVRAIARDLGRMLDAR
HHHHHHCCCEEEECCCCEEEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCC
GHVSALRRACVGPFLEEDLVPLDEMRERAEAGEEALMDALDPVALALEEIPQIAVTPPDA
HHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCH
HRLRCGQSVILRGRDAPIVQGHAAITCQGALIAIGDVDAGEIFPHRVFNWGQKDKKPPRR
HHHHCCCEEEEECCCCCEEECCEEEEECCEEEEEECCCCCCCCHHHHHCCCCCCCCCCCC
GKR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA