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The map label for this gene is pnp

Identifier: 154244245

GI number: 154244245

Start: 291508

End: 293700

Strand: Reverse

Name: pnp

Synonym: Xaut_0288

Alternate gene names: 154244245

Gene position: 293700-291508 (Counterclockwise)

Preceding gene: 154244246

Following gene: 154244244

Centisome position: 5.53

GC content: 67.17

Gene sequence:

>2193_bases
ATGTTCGACATCCATCGCGAAGAGCTGGACTGGGGTGGCCGCACCCTCACCCTCGAGACCGGCAAGATGGCCCGCCAGGC
CGACGGCTCGGTGCTCGCCACCTACGGCGACACCAAGGTGCTCGCCACCGTAGTTTCCGCCCGCGAGCCCAAGCCCGGCC
AGGACTTCTTCCCCCTGACCGTGAACTACCAGGAAAAGACCTACGCCGCGGGCCGCATCCCCGGCGGCTATTTCAAGCGC
GAGGGCCGTCCCTCCGAGAAGGAGACCCTGGTCTCCCGCCTCATCGACCGCCCGATCCGCCCGCTGTTCCCCGAAGGCTA
CAAGTGCGACACCCAGGTGGTCATCACCGTGCTGGCGCATGACCTGGAGAACGACCCCGACGTGGTCGCCATGGTCGCCG
CCTCGGCCGCCCTCACCCTCTCCGGCGTGCCCTTCATGGGCCCGGTGGGCGCTGCGCGCGTGGGCTTCATTGACAACGAG
TACGTGCTCAACCCCACCGTCGACGAGGTGAAGGAAAGCGCGCTTGAGCTCGTGGTCGCCGGCACGGGCGACGCCGTGCT
CATGGTGGAATCGGAAGCCAAGGAGCTGCCCGAGGAGATCATGCTCGGCGCCGTGATGTTCGGCCACCGCCACTTCCAGC
CGGTGATCGAGGCCATCATCAAGCTGGCCGAGAAGGCCGCCAAGGAGCCGCGCAACTTCCAGCCGGCCGATGTCTCCGAA
GTGGAGTCCAAGGTCCGCGAGATCGCCGAGGCCGACCTGCGCGCCGCCTACAAGATCAAGCAGAAGCAGGACCGCTACGC
CGCCGTCGGCGCCGCCAAGTCCAAGGTGAAGAAGTATTACGAGGAACTGGCGCTCGACGGCACCAAGGTGCCGACCGCCC
AGGTCGTCTCCGACGTGCTCAAGGCGCTCGAAGCCAAGATCGTGCGCTGGAACATCCTCGACGACGGCATCCGCATCGAC
GGCCGCGACGTCTACACCGTGCGTCCCATCGTGTCGGAAGTGGGCATCCTGCCCCGCGCCCACGGCTCCGCTTTGTTCAC
CCGCGGCGAGACGCAGGCCCTGGTCGTCGCCACGCTGGGCACCGGCGAGGACGAGCAGTTCATCGACAGCCTGGAAGGCA
CCTACAAGGAGCACTTCCTGCTGCATTACAACTTCCCGCCCTTCTCCGTGGGCGAGACCGGCCGCATGGGCTCCCCCGGC
CGCCGCGAGATCGGCCACGGCAAGCTCGCCTGGCGCGCCATCCACCCCATGCTGCCGGCCAAGCACGAGTTCCCCTACAC
CCTGCGCGTGGTGTCGGAGATCCTCGAGTCCAACGGCTCGTCCTCCATGGCCACCGTCTGCGGCACCTCGCTGGCGCTGA
TGGATGCGGGCGTGCCGCTGCGCCGCCCGGTGGCGGGCATCGCCATGGGCCTCATCCTCGAGGGTGAGAAGTTCGCCGTG
CTCTCCGACATCCTCGGCGACGAGGATCACCTCGGCGACATGGACTTCAAGGTGGCCGGCACCGAGCAGGGCGTGACCTC
GCTCCAGATGGACATCAAGATCGCCGGCATCACCGAGGAGATCATGAAGGTCGCCCTCACCCAGGCGAAGGACGGCCGCG
TCCACATCCTCGGCGAGATGGCCAAGGCCCTCACCACCGCCCGCGCGGAACTCGGCGAGCACGCCCCGCGCATCGAGGTG
ATGAAGATCGCCGTGGACAAGATCCGCGAAGTGATCGGCTCCGGCGGCAAGGTGATCCGCGAGATCGTCGAGAAGACCGG
CGCCAAGATCAACATCGAGGACGACGGCACCATCAAGATCGCCTCGGCCTCGGGCGACGCCATCAAGGCCGCCATCAACT
GGATCAAGTCCATCGCTTCCGAGCCGGAAGTGGGACAGATCTATGAAGGCACCGTCGTGAAGGTGGTGGACTTCGGCGCC
TTCGTGAACTTCTTCGGCTCCAAGGACGGCCTCGTCCACGTGTCGCAGATGGCCAACGAGCGCGTGGCCAAGCCCTCCGA
CGTGGTCAAGGAAGGCGACAAGGTCAAGGTCAAGCTCATGGGCTTCGACGAGCGCGGCAAGACCCGCCTGTCCATGAAGG
TGGTGGACCAGACCACCGGCGAGGACCTGGAAGCCAAGGCCAAGGCCGAGCGCGACGCCGCCCGTGCCGCCGCCCCGGCG
GCCACCGGCGACGAGGCCGGCGCTGCCGAGTGA

Upstream 100 bases:

>100_bases
ACCGTCGGAGCCTCCTCTTGAGGGAGCAGCTTCGCGCGGCGCGTCCGGCCGTCTTGCTCATGGCTTGCCTGCGTCGCGCC
TGAATCAAGAAAGAACACTC

Downstream 100 bases:

>100_bases
TCCGGCACTGATCTGAAGCAAGCTGAAGGGCGGCCGAGAGGCCGCCCTTTTTGCTGGGTGCAGCCATGGCCGCGAGAATC
GGAACGTCCCGGCCAAGGGT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 730; Mature: 730

Protein sequence:

>730_residues
MFDIHREELDWGGRTLTLETGKMARQADGSVLATYGDTKVLATVVSAREPKPGQDFFPLTVNYQEKTYAAGRIPGGYFKR
EGRPSEKETLVSRLIDRPIRPLFPEGYKCDTQVVITVLAHDLENDPDVVAMVAASAALTLSGVPFMGPVGAARVGFIDNE
YVLNPTVDEVKESALELVVAGTGDAVLMVESEAKELPEEIMLGAVMFGHRHFQPVIEAIIKLAEKAAKEPRNFQPADVSE
VESKVREIAEADLRAAYKIKQKQDRYAAVGAAKSKVKKYYEELALDGTKVPTAQVVSDVLKALEAKIVRWNILDDGIRID
GRDVYTVRPIVSEVGILPRAHGSALFTRGETQALVVATLGTGEDEQFIDSLEGTYKEHFLLHYNFPPFSVGETGRMGSPG
RREIGHGKLAWRAIHPMLPAKHEFPYTLRVVSEILESNGSSSMATVCGTSLALMDAGVPLRRPVAGIAMGLILEGEKFAV
LSDILGDEDHLGDMDFKVAGTEQGVTSLQMDIKIAGITEEIMKVALTQAKDGRVHILGEMAKALTTARAELGEHAPRIEV
MKIAVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTIKIASASGDAIKAAINWIKSIASEPEVGQIYEGTVVKVVDFGA
FVNFFGSKDGLVHVSQMANERVAKPSDVVKEGDKVKVKLMGFDERGKTRLSMKVVDQTTGEDLEAKAKAERDAARAAAPA
ATGDEAGAAE

Sequences:

>Translated_730_residues
MFDIHREELDWGGRTLTLETGKMARQADGSVLATYGDTKVLATVVSAREPKPGQDFFPLTVNYQEKTYAAGRIPGGYFKR
EGRPSEKETLVSRLIDRPIRPLFPEGYKCDTQVVITVLAHDLENDPDVVAMVAASAALTLSGVPFMGPVGAARVGFIDNE
YVLNPTVDEVKESALELVVAGTGDAVLMVESEAKELPEEIMLGAVMFGHRHFQPVIEAIIKLAEKAAKEPRNFQPADVSE
VESKVREIAEADLRAAYKIKQKQDRYAAVGAAKSKVKKYYEELALDGTKVPTAQVVSDVLKALEAKIVRWNILDDGIRID
GRDVYTVRPIVSEVGILPRAHGSALFTRGETQALVVATLGTGEDEQFIDSLEGTYKEHFLLHYNFPPFSVGETGRMGSPG
RREIGHGKLAWRAIHPMLPAKHEFPYTLRVVSEILESNGSSSMATVCGTSLALMDAGVPLRRPVAGIAMGLILEGEKFAV
LSDILGDEDHLGDMDFKVAGTEQGVTSLQMDIKIAGITEEIMKVALTQAKDGRVHILGEMAKALTTARAELGEHAPRIEV
MKIAVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTIKIASASGDAIKAAINWIKSIASEPEVGQIYEGTVVKVVDFGA
FVNFFGSKDGLVHVSQMANERVAKPSDVVKEGDKVKVKLMGFDERGKTRLSMKVVDQTTGEDLEAKAKAERDAARAAAPA
ATGDEAGAAE
>Mature_730_residues
MFDIHREELDWGGRTLTLETGKMARQADGSVLATYGDTKVLATVVSAREPKPGQDFFPLTVNYQEKTYAAGRIPGGYFKR
EGRPSEKETLVSRLIDRPIRPLFPEGYKCDTQVVITVLAHDLENDPDVVAMVAASAALTLSGVPFMGPVGAARVGFIDNE
YVLNPTVDEVKESALELVVAGTGDAVLMVESEAKELPEEIMLGAVMFGHRHFQPVIEAIIKLAEKAAKEPRNFQPADVSE
VESKVREIAEADLRAAYKIKQKQDRYAAVGAAKSKVKKYYEELALDGTKVPTAQVVSDVLKALEAKIVRWNILDDGIRID
GRDVYTVRPIVSEVGILPRAHGSALFTRGETQALVVATLGTGEDEQFIDSLEGTYKEHFLLHYNFPPFSVGETGRMGSPG
RREIGHGKLAWRAIHPMLPAKHEFPYTLRVVSEILESNGSSSMATVCGTSLALMDAGVPLRRPVAGIAMGLILEGEKFAV
LSDILGDEDHLGDMDFKVAGTEQGVTSLQMDIKIAGITEEIMKVALTQAKDGRVHILGEMAKALTTARAELGEHAPRIEV
MKIAVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTIKIASASGDAIKAAINWIKSIASEPEVGQIYEGTVVKVVDFGA
FVNFFGSKDGLVHVSQMANERVAKPSDVVKEGDKVKVKLMGFDERGKTRLSMKVVDQTTGEDLEAKAKAERDAARAAAPA
ATGDEAGAAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=715, Percent_Identity=38.4615384615385, Blast_Score=465, Evalue=1e-131,
Organism=Homo sapiens, GI4826690, Length=92, Percent_Identity=47.8260869565217, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI21361576, Length=93, Percent_Identity=46.2365591397849, Blast_Score=77, Evalue=7e-14,
Organism=Escherichia coli, GI145693187, Length=689, Percent_Identity=57.6197387518142, Blast_Score=787, Evalue=0.0,
Organism=Escherichia coli, GI87082262, Length=78, Percent_Identity=46.1538461538462, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI115534063, Length=709, Percent_Identity=33.9915373765867, Blast_Score=359, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI17535281, Length=87, Percent_Identity=49.4252873563218, Blast_Score=82, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320850, Length=124, Percent_Identity=33.8709677419355, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281362905, Length=713, Percent_Identity=38.4291725105189, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651641, Length=713, Percent_Identity=38.4291725105189, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651643, Length=713, Percent_Identity=38.4291725105189, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI161079377, Length=663, Percent_Identity=37.8582202111614, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI20129977, Length=100, Percent_Identity=42, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_XANP2 (A7IC03)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001415203.1
- ProteinModelPortal:   A7IC03
- SMR:   A7IC03
- STRING:   A7IC03
- GeneID:   5422788
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_0288
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   XAUT78245:XAUT_0288-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 78914; Mature: 78914

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDIHREELDWGGRTLTLETGKMARQADGSVLATYGDTKVLATVVSAREPKPGQDFFPLT
CCCCCCHHCCCCCEEEEEECCCHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCCEEEE
VNYQEKTYAAGRIPGGYFKREGRPSEKETLVSRLIDRPIRPLFPEGYKCDTQVVITVLAH
EECCHHEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
DLENDPDVVAMVAASAALTLSGVPFMGPVGAARVGFIDNEYVLNPTVDEVKESALELVVA
HCCCCCCHHHHHHHHHHHEECCCCCCCCCCCCEEEECCCCEEECCCHHHHHHHHHEEEEE
GTGDAVLMVESEAKELPEEIMLGAVMFGHRHFQPVIEAIIKLAEKAAKEPRNFQPADVSE
CCCCEEEEECCHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VESKVREIAEADLRAAYKIKQKQDRYAAVGAAKSKVKKYYEELALDGTKVPTAQVVSDVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
KALEAKIVRWNILDDGIRIDGRDVYTVRPIVSEVGILPRAHGSALFTRGETQALVVATLG
HHHHHHEEEEEEECCCEEECCCEEEEECHHHHHHCCCCCCCCCEEEECCCCCEEEEEEEC
TGEDEQFIDSLEGTYKEHFLLHYNFPPFSVGETGRMGSPGRREIGHGKLAWRAIHPMLPA
CCCHHHHHHHHCCCCHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCC
KHEFPYTLRVVSEILESNGSSSMATVCGTSLALMDAGVPLRRPVAGIAMGLILEGEKFAV
CCCCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHCCCCCCCCHHHHHHHHEECCCCHHH
LSDILGDEDHLGDMDFKVAGTEQGVTSLQMDIKIAGITEEIMKVALTQAKDGRVHILGEM
HHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHCCCCCCEEEHHHH
AKALTTARAELGEHAPRIEVMKIAVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTIKI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEE
ASASGDAIKAAINWIKSIASEPEVGQIYEGTVVKVVDFGAFVNFFGSKDGLVHVSQMANE
EECCCHHHHHHHHHHHHHHCCCCCCCEECCCEEEEEHHHHHHHHHCCCCCEEEHHHHHHH
RVAKPSDVVKEGDKVKVKLMGFDERGKTRLSMKVVDQTTGEDLEAKAKAERDAARAAAPA
HCCCCHHHHCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
ATGDEAGAAE
CCCCCCCCCC
>Mature Secondary Structure
MFDIHREELDWGGRTLTLETGKMARQADGSVLATYGDTKVLATVVSAREPKPGQDFFPLT
CCCCCCHHCCCCCEEEEEECCCHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCCEEEE
VNYQEKTYAAGRIPGGYFKREGRPSEKETLVSRLIDRPIRPLFPEGYKCDTQVVITVLAH
EECCHHEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
DLENDPDVVAMVAASAALTLSGVPFMGPVGAARVGFIDNEYVLNPTVDEVKESALELVVA
HCCCCCCHHHHHHHHHHHEECCCCCCCCCCCCEEEECCCCEEECCCHHHHHHHHHEEEEE
GTGDAVLMVESEAKELPEEIMLGAVMFGHRHFQPVIEAIIKLAEKAAKEPRNFQPADVSE
CCCCEEEEECCHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VESKVREIAEADLRAAYKIKQKQDRYAAVGAAKSKVKKYYEELALDGTKVPTAQVVSDVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
KALEAKIVRWNILDDGIRIDGRDVYTVRPIVSEVGILPRAHGSALFTRGETQALVVATLG
HHHHHHEEEEEEECCCEEECCCEEEEECHHHHHHCCCCCCCCCEEEECCCCCEEEEEEEC
TGEDEQFIDSLEGTYKEHFLLHYNFPPFSVGETGRMGSPGRREIGHGKLAWRAIHPMLPA
CCCHHHHHHHHCCCCHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCC
KHEFPYTLRVVSEILESNGSSSMATVCGTSLALMDAGVPLRRPVAGIAMGLILEGEKFAV
CCCCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHCCCCCCCCHHHHHHHHEECCCCHHH
LSDILGDEDHLGDMDFKVAGTEQGVTSLQMDIKIAGITEEIMKVALTQAKDGRVHILGEM
HHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHCCCCCCEEEHHHH
AKALTTARAELGEHAPRIEVMKIAVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTIKI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEE
ASASGDAIKAAINWIKSIASEPEVGQIYEGTVVKVVDFGAFVNFFGSKDGLVHVSQMANE
EECCCHHHHHHHHHHHHHHCCCCCCCEECCCEEEEEHHHHHHHHHCCCCCEEEHHHHHHH
RVAKPSDVVKEGDKVKVKLMGFDERGKTRLSMKVVDQTTGEDLEAKAKAERDAARAAAPA
HCCCCHHHHCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
ATGDEAGAAE
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA