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The map label for this gene is fabI2 [H]

Identifier: 154244010

GI number: 154244010

Start: 55577

End: 56425

Strand: Reverse

Name: fabI2 [H]

Synonym: Xaut_0052

Alternate gene names: 154244010

Gene position: 56425-55577 (Counterclockwise)

Preceding gene: 154244011

Following gene: 154244009

Centisome position: 1.06

GC content: 66.2

Gene sequence:

>849_bases
ATGCAGGACCTGATGAAGGGCAAGCGTGGCTTGGTGATGGGGGTCGCGAACGACCATTCCATCGCCTGGGGCATCGCCAA
AGCCTTGGCCGGCCAGGGCGCCGAGATCGCCTTCACCTACCAGGGCGATTCCGTGGCCAAGCGCGTGAAGCCGCTGGCCG
AGAGCCTCGGCTCCTCCATCCTCCTGCCGTGCGACGTGGAAGACCTCGCCAGCGTGGATGCCGTGTTCGCGGCGCTGAAG
GAGCGCTGGGGCACCATCGATTTCCTGGTGCATGCGGTGGCCTTCTCCGACAAGTCGGAGCTGAAGGGCCGCTACGCTGA
TACCAGCCGAGAGAATTTCTCCCGCACCATGGTCATCTCCTGCTTCTCCTTCACGGAGATCGCCAAGCGCGCCGCCGCGC
TGATGCCCAATGGCGGGTCGCTCGTCACCCTGACCTATGGCGGCTCCACCCGCGTCATGCCCAATTACAACGTGATGGGC
GTGGCCAAGGCGGCGCTGGAAGCTTCGGTGCGCTACCTCGCCGCCGATTACGGGCCGGCCGGCATCCGCGTGAACGCCAT
TTCGGCGGGGCCCGTACGCACGCTCGCCGGCGCCGGCATCGCTGATGCCCGCCTGATGTTCAACTACCAGAAGCGCCACG
CCCCCCTGCGCCGCACCGTCTCCATCGAGGAAGTGGGCGGCTCCGCCCTCTACCTGCTGTCCGAGCTGTCGGGTGGCGTG
ACGGGCGAGATCCATTTCGTGGATTCCGGCTACAACATCATGTCCATGCCGCATCCCGACGTGCTCAAGCAGCAGGAAGA
CGCCGAGGCGAAGCTTGCGGCCGACGCGCCGGTGAAGGCTGCGGAATAG

Upstream 100 bases:

>100_bases
AGAGGCATGCGTCAGGGTCGGTCATGAAAGGTGAAGGGCCGTGCCGTGGTGCCGGCCCGCGCGGTCTGCTATCGGCGTTT
CAGGCGCGAGAGGGTGGGTG

Downstream 100 bases:

>100_bases
CAGCAGCGCCCCGGGCCTGCGCCGGTGATCGACCCGACCTGACGCAGCGGCGGGCGCGATTGCCTCTTTACCTCCCGCGC
CGGAGCGCTACCTATCGGCG

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 2 [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MQDLMKGKRGLVMGVANDHSIAWGIAKALAGQGAEIAFTYQGDSVAKRVKPLAESLGSSILLPCDVEDLASVDAVFAALK
ERWGTIDFLVHAVAFSDKSELKGRYADTSRENFSRTMVISCFSFTEIAKRAAALMPNGGSLVTLTYGGSTRVMPNYNVMG
VAKAALEASVRYLAADYGPAGIRVNAISAGPVRTLAGAGIADARLMFNYQKRHAPLRRTVSIEEVGGSALYLLSELSGGV
TGEIHFVDSGYNIMSMPHPDVLKQQEDAEAKLAADAPVKAAE

Sequences:

>Translated_282_residues
MQDLMKGKRGLVMGVANDHSIAWGIAKALAGQGAEIAFTYQGDSVAKRVKPLAESLGSSILLPCDVEDLASVDAVFAALK
ERWGTIDFLVHAVAFSDKSELKGRYADTSRENFSRTMVISCFSFTEIAKRAAALMPNGGSLVTLTYGGSTRVMPNYNVMG
VAKAALEASVRYLAADYGPAGIRVNAISAGPVRTLAGAGIADARLMFNYQKRHAPLRRTVSIEEVGGSALYLLSELSGGV
TGEIHFVDSGYNIMSMPHPDVLKQQEDAEAKLAADAPVKAAE
>Mature_282_residues
MQDLMKGKRGLVMGVANDHSIAWGIAKALAGQGAEIAFTYQGDSVAKRVKPLAESLGSSILLPCDVEDLASVDAVFAALK
ERWGTIDFLVHAVAFSDKSELKGRYADTSRENFSRTMVISCFSFTEIAKRAAALMPNGGSLVTLTYGGSTRVMPNYNVMG
VAKAALEASVRYLAADYGPAGIRVNAISAGPVRTLAGAGIADARLMFNYQKRHAPLRRTVSIEEVGGSALYLLSELSGGV
TGEIHFVDSGYNIMSMPHPDVLKQQEDAEAKLAADAPVKAAE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=254, Percent_Identity=49.6062992125984, Blast_Score=248, Evalue=2e-67,
Organism=Escherichia coli, GI1789378, Length=259, Percent_Identity=28.1853281853282, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI87082100, Length=261, Percent_Identity=27.2030651340996, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI2367365, Length=249, Percent_Identity=26.9076305220884, Blast_Score=66, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324126, Length=261, Percent_Identity=26.0536398467433, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24644337, Length=191, Percent_Identity=30.8900523560209, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 29918; Mature: 29918

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDLMKGKRGLVMGVANDHSIAWGIAKALAGQGAEIAFTYQGDSVAKRVKPLAESLGSSI
CCHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCCE
LLPCDVEDLASVDAVFAALKERWGTIDFLVHAVAFSDKSELKGRYADTSRENFSRTMVIS
EEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHCCCCCCCCHHCCCEEEEEE
CFSFTEIAKRAAALMPNGGSLVTLTYGGSTRVMPNYNVMGVAKAALEASVRYLAADYGPA
HHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHEECCCCC
GIRVNAISAGPVRTLAGAGIADARLMFNYQKRHAPLRRTVSIEEVGGSALYLLSELSGGV
CEEEEEECCCCHHHHHCCCCCHHHHEEEHHHHCCCHHHEEEHHHCCCHHEEHHHHHCCCC
TGEIHFVDSGYNIMSMPHPDVLKQQEDAEAKLAADAPVKAAE
EEEEEEEECCCEEEECCCHHHHCCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure
MQDLMKGKRGLVMGVANDHSIAWGIAKALAGQGAEIAFTYQGDSVAKRVKPLAESLGSSI
CCHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCCE
LLPCDVEDLASVDAVFAALKERWGTIDFLVHAVAFSDKSELKGRYADTSRENFSRTMVIS
EEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHCCCCCCCCHHCCCEEEEEE
CFSFTEIAKRAAALMPNGGSLVTLTYGGSTRVMPNYNVMGVAKAALEASVRYLAADYGPA
HHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHEECCCCC
GIRVNAISAGPVRTLAGAGIADARLMFNYQKRHAPLRRTVSIEEVGGSALYLLSELSGGV
CEEEEEECCCCHHHHHCCCCCHHHHEEEHHHHCCCHHHEEEHHHCCCHHEEHHHHHCCCC
TGEIHFVDSGYNIMSMPHPDVLKQQEDAEAKLAADAPVKAAE
EEEEEEEECCCEEEECCCHHHHCCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]