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The map label for this gene is livJ [C]

Identifier: 154244000

GI number: 154244000

Start: 45014

End: 46186

Strand: Reverse

Name: livJ [C]

Synonym: Xaut_0042

Alternate gene names: 154244000

Gene position: 46186-45014 (Counterclockwise)

Preceding gene: 154244001

Following gene: 154243999

Centisome position: 0.87

GC content: 66.75

Gene sequence:

>1173_bases
ATGAAGAAAAGTCTGCTTCTTGGCGCCGGCCTCCTGGCCGCGCTGGTCACCGCCGGCACCGCGCGGGCGGAGGAAGAGCT
CGTCCTCGGCTACATGATGGCCAAGAGCGGCCCCTATGTGAGCCTTGCCAACACCAATGAGGTGGCGGTGGACATGGCGG
TCGAGGAAATCAACGCCAAGGGCGGCATCAACGGCAAGAAGATCAAGGTGGTGAAGTTCGACACCGCCGGCGATCCCAAG
CAGGCCACCACCGCCGTGCGCGCGTTCGCGCAGGATGCCGGGGCGCTGGCCATCATCGGGCCGTTCTCCTCCTCGGAAGT
GCGCACCACCTTCCCGGTGGGCGAGCGCGAGGGCATCTCGCAGATGTCCATGGCCTCCTCGGCGCCGGGCCTCACCAAGG
GCTTCACCTACGGCTTCCGCAACACCACCGACGAAGGCAAGGTGATCGACCAGGTGCTGGCGTCCATCAAGGACAAGAAG
CTGCCCATGGCCTCGGGCGCGGCGGCCTATGCCACCGACGACGTGGTCTCCAAATCCATCGGCACCGGCGTGGTGCCCAA
GATGTTCGAGAAGTATTCGATCCCCTCCAAGGGCGCCGTGGACTTCCAGCTCGCCGCCTTCGACCTGTCGCCCCAGGTGG
CGCAGTTGAAGCAGATGGCGCCCGACGTGGTCGGCCTCGGCTCGCCGCCGGAAGGCGCCATCACCATCGCCAAGGAATTG
AAGCGCCAGGGCGTCTCCACCCGCCTCATCGGCGGCACCACCATCGCCGATCCCGAGCTGCCCAAGCGCATGGATGGCGC
CGGCGAGACGCTCACCATCGGCACCACCTTCTTCCCCGAGGTGAACGACAAGACCCGCGCCTTCGCGGCGGAATTCGCCA
AGCGCACCAAGGCCGCAGGCCTCAACCGCACCGACCCCAACCAGATGGACGCCTCGGTCTACGACATCGTCTACCTCTAC
GCCGAGGCCATGAAGCGGGCCGGCACCACCGGCGACAAGGCCAAGCTCGCCGCCGAGCGCAGCGCGGTCCGCGACCAGGT
GGCCAAGCTGAAGGACGTGCCGGCGCTCGAAGGCGCCATCTCCTTCGCCGACGGCGACGCCGTGAAGCCCGTCTACATCC
TTGAGGTGAAGGGCGGGAAGTGGACCCTTCTGGATACGCGGATGCCGAACTGA

Upstream 100 bases:

>100_bases
AATGCCCACTGATCTTTTCAAGTGAAGTGAAGACCGGTTCGCGTGACGAAAACACGCGCGACACACAGAAAAACCAGAGC
AATCCAGAGGAGAATTGGAC

Downstream 100 bases:

>100_bases
CCTCGGCACACTCCGCGTACGGCCGGCGCCTTCCTCCTGCCCCTGACCGGAAGGCGCCGGCTCTTCTTCCATTCCCTCGA
AAGACACATCCATGCCGGGC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKKSLLLGAGLLAALVTAGTARAEEELVLGYMMAKSGPYVSLANTNEVAVDMAVEEINAKGGINGKKIKVVKFDTAGDPK
QATTAVRAFAQDAGALAIIGPFSSSEVRTTFPVGEREGISQMSMASSAPGLTKGFTYGFRNTTDEGKVIDQVLASIKDKK
LPMASGAAAYATDDVVSKSIGTGVVPKMFEKYSIPSKGAVDFQLAAFDLSPQVAQLKQMAPDVVGLGSPPEGAITIAKEL
KRQGVSTRLIGGTTIADPELPKRMDGAGETLTIGTTFFPEVNDKTRAFAAEFAKRTKAAGLNRTDPNQMDASVYDIVYLY
AEAMKRAGTTGDKAKLAAERSAVRDQVAKLKDVPALEGAISFADGDAVKPVYILEVKGGKWTLLDTRMPN

Sequences:

>Translated_390_residues
MKKSLLLGAGLLAALVTAGTARAEEELVLGYMMAKSGPYVSLANTNEVAVDMAVEEINAKGGINGKKIKVVKFDTAGDPK
QATTAVRAFAQDAGALAIIGPFSSSEVRTTFPVGEREGISQMSMASSAPGLTKGFTYGFRNTTDEGKVIDQVLASIKDKK
LPMASGAAAYATDDVVSKSIGTGVVPKMFEKYSIPSKGAVDFQLAAFDLSPQVAQLKQMAPDVVGLGSPPEGAITIAKEL
KRQGVSTRLIGGTTIADPELPKRMDGAGETLTIGTTFFPEVNDKTRAFAAEFAKRTKAAGLNRTDPNQMDASVYDIVYLY
AEAMKRAGTTGDKAKLAAERSAVRDQVAKLKDVPALEGAISFADGDAVKPVYILEVKGGKWTLLDTRMPN
>Mature_390_residues
MKKSLLLGAGLLAALVTAGTARAEEELVLGYMMAKSGPYVSLANTNEVAVDMAVEEINAKGGINGKKIKVVKFDTAGDPK
QATTAVRAFAQDAGALAIIGPFSSSEVRTTFPVGEREGISQMSMASSAPGLTKGFTYGFRNTTDEGKVIDQVLASIKDKK
LPMASGAAAYATDDVVSKSIGTGVVPKMFEKYSIPSKGAVDFQLAAFDLSPQVAQLKQMAPDVVGLGSPPEGAITIAKEL
KRQGVSTRLIGGTTIADPELPKRMDGAGETLTIGTTFFPEVNDKTRAFAAEFAKRTKAAGLNRTDPNQMDASVYDIVYLY
AEAMKRAGTTGDKAKLAAERSAVRDQVAKLKDVPALEGAISFADGDAVKPVYILEVKGGKWTLLDTRMPN

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=284, Percent_Identity=29.2253521126761, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789867, Length=133, Percent_Identity=33.8345864661654, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41026; Mature: 41026

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSLLLGAGLLAALVTAGTARAEEELVLGYMMAKSGPYVSLANTNEVAVDMAVEEINAK
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCCCEEEEHHHHHHCCC
GGINGKKIKVVKFDTAGDPKQATTAVRAFAQDAGALAIIGPFSSSEVRTTFPVGEREGIS
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCCCH
QMSMASSAPGLTKGFTYGFRNTTDEGKVIDQVLASIKDKKLPMASGAAAYATDDVVSKSI
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHC
GTGVVPKMFEKYSIPSKGAVDFQLAAFDLSPQVAQLKQMAPDVVGLGSPPEGAITIAKEL
CCCCHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCEECCCCCCCHHHHHHHH
KRQGVSTRLIGGTTIADPELPKRMDGAGETLTIGTTFFPEVNDKTRAFAAEFAKRTKAAG
HHCCCCEEEECCCCCCCCCCCHHHCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHHCC
LNRTDPNQMDASVYDIVYLYAEAMKRAGTTGDKAKLAAERSAVRDQVAKLKDVPALEGAI
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCEE
SFADGDAVKPVYILEVKGGKWTLLDTRMPN
EECCCCCCCCEEEEEECCCEEEEECCCCCC
>Mature Secondary Structure
MKKSLLLGAGLLAALVTAGTARAEEELVLGYMMAKSGPYVSLANTNEVAVDMAVEEINAK
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCCCEEEEHHHHHHCCC
GGINGKKIKVVKFDTAGDPKQATTAVRAFAQDAGALAIIGPFSSSEVRTTFPVGEREGIS
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCCCH
QMSMASSAPGLTKGFTYGFRNTTDEGKVIDQVLASIKDKKLPMASGAAAYATDDVVSKSI
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHC
GTGVVPKMFEKYSIPSKGAVDFQLAAFDLSPQVAQLKQMAPDVVGLGSPPEGAITIAKEL
CCCCHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCEECCCCCCCHHHHHHHH
KRQGVSTRLIGGTTIADPELPKRMDGAGETLTIGTTFFPEVNDKTRAFAAEFAKRTKAAG
HHCCCCEEEECCCCCCCCCCCHHHCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHHCC
LNRTDPNQMDASVYDIVYLYAEAMKRAGTTGDKAKLAAERSAVRDQVAKLKDVPALEGAI
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCEE
SFADGDAVKPVYILEVKGGKWTLLDTRMPN
EECCCCCCCCEEEEEECCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA