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The map label for this gene is purA [H]

Identifier: 154243995

GI number: 154243995

Start: 38706

End: 40052

Strand: Reverse

Name: purA [H]

Synonym: Xaut_0037

Alternate gene names: 154243995

Gene position: 40052-38706 (Counterclockwise)

Preceding gene: 154243998

Following gene: 154243994

Centisome position: 0.75

GC content: 68.75

Gene sequence:

>1347_bases
GTGCGCGCGTGCGTTGCGATGCACCCGCGCCGGACCGGGAGGCGCCTTAAGGTGGCCAACGTGGTGGTAGTCGGCTCCCA
ATGGGGCGACGAAGGCAAGGGCAAGATCGTCGATTGGCTGTCGGAGCAGGCGGACGTGGTGGTCCGCTTCCAGGGCGGCC
ACAATGCGGGCCATACGCTGGTCGTCGGCGGCGTCACCTACAAGCTCTCCCTTTTGCCTTCGGGCGTGGTGCGCGGCGGC
AAGCTCTCCGTGATCGGCAATGGCGTGGTGCTGGACCCGCAGGCGCTGGTGGACGAACTCGCCCGCCTCGCCGCCCAGGG
GGTCGAGATCGGTCCCGACCGGCTGCGCATCGCCGAAACCGTTCCGCTCATCCTGCCGCTGCACCGCGAGCTGGACGCCC
TGCGCGAGAGCGCCACCGCCGAGGGTGCGCGTATCGGCACCACCAAGCGCGGCATCGGCCCGGCCTATGAGGACAAGGTA
GGCCGTCGCGCCATCCGCCTCGTGGACCTCTCCGACCCGTCCACCCTGCCGGCCAAGGTGGATCGGCTGCTGACCCACCA
CAACCTCATCCGCCGCGGCCTCGGCATGGAAGAGGTGAACCGCGACGCCCTGGTGGCCGAGCTGCTCGCCATCGCCCCCA
AGGTCCTGCCCTTCATGGACCGGGTGTGGGAGCTGCTCGACAAGGCGCGGCGCGACGGCAAGAAGATCCTGTTCGAGGGC
GCGCAGGGCGCGCTGCTCGACATCGACCACGGCACCTATCCTTACGTCACCTCGTCCAACACGGTGGCGGGATCGGCGGC
GAGCGGCACCGGCATCGGCCCGGGCGCTCTGGATTATGTGCTCGGCATCACCAAGGCCTACACCACCCGCGTGGGCGAAG
GCCCCTTCCCCACCGAGCTGACCGACGAAGTGGGCCAGACCCTGGGCAGCAAGGGCCGCGAGTTCGGCGTGGTCACCGGC
CGTGCCCGCCGCTGCGGCTGGTTCGACGCGGTACTGGTGCGCCAGACGGTGCGCACCTGTGGCATCCACGGCATCGCCCT
CACCAAGCTCGACGTGCTGGACGGGTTTACCGAACTGAAGGTATGCGTCGGCTACAGGGTTGACGGTAAGGAAATCGATT
ACCTGCCGGCGGAGAGCGGGGCGCAGGCGCGCGCCGAGCCCATCTACGAGACCATGGAAGGCTGGCAGGATTCCACCGCT
GGCGCCCGCTCGTGGGCGGATCTGCCGGCGGAGGCGGTAAAGTATGTGCGACGGGTGGAGGAGCTCATCGGCTGTCCGGT
GGCCGTGCTCTCCACCAGCCCGGAGCGCGATGATACCATTCTGGTCCACAATCCGTTTCAGGCCTGA

Upstream 100 bases:

>100_bases
CCTTCCGCACGCGCGCTGCGCCTCGTTTACCTTGCCTCGCCAGCGCCGATCCCGTAAGACGCATTCGTAACGCCCCGGAC
CTGAGCCGGGGCTTTCCTTT

Downstream 100 bases:

>100_bases
TCTTCGGGCGTGACGCGGATCCGGAGCATTTCACGCGGCGCGAACTGCGGTTCGCGTCCAGGAAATGCAGCATATAAATG
CGATAGGGCGTCTTTCGTGA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MRACVAMHPRRTGRRLKVANVVVVGSQWGDEGKGKIVDWLSEQADVVVRFQGGHNAGHTLVVGGVTYKLSLLPSGVVRGG
KLSVIGNGVVLDPQALVDELARLAAQGVEIGPDRLRIAETVPLILPLHRELDALRESATAEGARIGTTKRGIGPAYEDKV
GRRAIRLVDLSDPSTLPAKVDRLLTHHNLIRRGLGMEEVNRDALVAELLAIAPKVLPFMDRVWELLDKARRDGKKILFEG
AQGALLDIDHGTYPYVTSSNTVAGSAASGTGIGPGALDYVLGITKAYTTRVGEGPFPTELTDEVGQTLGSKGREFGVVTG
RARRCGWFDAVLVRQTVRTCGIHGIALTKLDVLDGFTELKVCVGYRVDGKEIDYLPAESGAQARAEPIYETMEGWQDSTA
GARSWADLPAEAVKYVRRVEELIGCPVAVLSTSPERDDTILVHNPFQA

Sequences:

>Translated_448_residues
MRACVAMHPRRTGRRLKVANVVVVGSQWGDEGKGKIVDWLSEQADVVVRFQGGHNAGHTLVVGGVTYKLSLLPSGVVRGG
KLSVIGNGVVLDPQALVDELARLAAQGVEIGPDRLRIAETVPLILPLHRELDALRESATAEGARIGTTKRGIGPAYEDKV
GRRAIRLVDLSDPSTLPAKVDRLLTHHNLIRRGLGMEEVNRDALVAELLAIAPKVLPFMDRVWELLDKARRDGKKILFEG
AQGALLDIDHGTYPYVTSSNTVAGSAASGTGIGPGALDYVLGITKAYTTRVGEGPFPTELTDEVGQTLGSKGREFGVVTG
RARRCGWFDAVLVRQTVRTCGIHGIALTKLDVLDGFTELKVCVGYRVDGKEIDYLPAESGAQARAEPIYETMEGWQDSTA
GARSWADLPAEAVKYVRRVEELIGCPVAVLSTSPERDDTILVHNPFQA
>Mature_448_residues
MRACVAMHPRRTGRRLKVANVVVVGSQWGDEGKGKIVDWLSEQADVVVRFQGGHNAGHTLVVGGVTYKLSLLPSGVVRGG
KLSVIGNGVVLDPQALVDELARLAAQGVEIGPDRLRIAETVPLILPLHRELDALRESATAEGARIGTTKRGIGPAYEDKV
GRRAIRLVDLSDPSTLPAKVDRLLTHHNLIRRGLGMEEVNRDALVAELLAIAPKVLPFMDRVWELLDKARRDGKKILFEG
AQGALLDIDHGTYPYVTSSNTVAGSAASGTGIGPGALDYVLGITKAYTTRVGEGPFPTELTDEVGQTLGSKGREFGVVTG
RARRCGWFDAVLVRQTVRTCGIHGIALTKLDVLDGFTELKVCVGYRVDGKEIDYLPAESGAQARAEPIYETMEGWQDSTA
GARSWADLPAEAVKYVRRVEELIGCPVAVLSTSPERDDTILVHNPFQA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=433, Percent_Identity=42.9561200923788, Blast_Score=338, Evalue=6e-93,
Organism=Homo sapiens, GI34577063, Length=425, Percent_Identity=42.3529411764706, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI40316944, Length=394, Percent_Identity=41.6243654822335, Blast_Score=296, Evalue=2e-80,
Organism=Escherichia coli, GI1790620, Length=431, Percent_Identity=52.2041763341067, Blast_Score=441, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI25146553, Length=443, Percent_Identity=42.2121896162528, Blast_Score=340, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI25146555, Length=428, Percent_Identity=42.9906542056075, Blast_Score=339, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6324109, Length=428, Percent_Identity=41.1214953271028, Blast_Score=325, Evalue=1e-89,
Organism=Drosophila melanogaster, GI21356033, Length=439, Percent_Identity=42.369020501139, Blast_Score=328, Evalue=4e-90,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 48227; Mature: 48227

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRACVAMHPRRTGRRLKVANVVVVGSQWGDEGKGKIVDWLSEQADVVVRFQGGHNAGHTL
CCCCEECCCCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCEE
VVGGVTYKLSLLPSGVVRGGKLSVIGNGVVLDPQALVDELARLAAQGVEIGPDRLRIAET
EEECEEEEEEECCCCCCCCCEEEEEECCEEECHHHHHHHHHHHHHCCCCCCHHHEEHHHH
VPLILPLHRELDALRESATAEGARIGTTKRGIGPAYEDKVGRRAIRLVDLSDPSTLPAKV
CCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCEEEEEEECCCCCCCHHHH
DRLLTHHNLIRRGLGMEEVNRDALVAELLAIAPKVLPFMDRVWELLDKARRDGKKILFEG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
AQGALLDIDHGTYPYVTSSNTVAGSAASGTGIGPGALDYVLGITKAYTTRVGEGPFPTEL
CCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHH
TDEVGQTLGSKGREFGVVTGRARRCGWFDAVLVRQTVRTCGIHGIALTKLDVLDGFTELK
HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHCCHHHEE
VCVGYRVDGKEIDYLPAESGAQARAEPIYETMEGWQDSTAGARSWADLPAEAVKYVRRVE
EEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHH
ELIGCPVAVLSTSPERDDTILVHNPFQA
HHHCCCEEEECCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MRACVAMHPRRTGRRLKVANVVVVGSQWGDEGKGKIVDWLSEQADVVVRFQGGHNAGHTL
CCCCEECCCCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCEE
VVGGVTYKLSLLPSGVVRGGKLSVIGNGVVLDPQALVDELARLAAQGVEIGPDRLRIAET
EEECEEEEEEECCCCCCCCCEEEEEECCEEECHHHHHHHHHHHHHCCCCCCHHHEEHHHH
VPLILPLHRELDALRESATAEGARIGTTKRGIGPAYEDKVGRRAIRLVDLSDPSTLPAKV
CCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCEEEEEEECCCCCCCHHHH
DRLLTHHNLIRRGLGMEEVNRDALVAELLAIAPKVLPFMDRVWELLDKARRDGKKILFEG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
AQGALLDIDHGTYPYVTSSNTVAGSAASGTGIGPGALDYVLGITKAYTTRVGEGPFPTEL
CCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHH
TDEVGQTLGSKGREFGVVTGRARRCGWFDAVLVRQTVRTCGIHGIALTKLDVLDGFTELK
HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHCCHHHEE
VCVGYRVDGKEIDYLPAESGAQARAEPIYETMEGWQDSTAGARSWADLPAEAVKYVRRVE
EEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHH
ELIGCPVAVLSTSPERDDTILVHNPFQA
HHHCCCEEEECCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA