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The map label for this gene is recF

Identifier: 154243961

GI number: 154243961

Start: 3580

End: 4716

Strand: Direct

Name: recF

Synonym: Xaut_0003

Alternate gene names: 154243961

Gene position: 3580-4716 (Clockwise)

Preceding gene: 154243960

Following gene: 154243962

Centisome position: 0.07

GC content: 72.56

Gene sequence:

>1137_bases
ATGCCCCACGCCGCCATCCGCAAGCTCACCCTCACGGCCTTCCGATCCTATCCATCGGCGCAGGTGAGCGTTGAGGACGG
GCCGGTGGTGCTCACCGGGCCGAACGGCGCGGGCAAGACCAATATTCTGGAAGCGCTCTCCTTCCTCTCTCCCGGCCGGG
GGCTGCGGCGTGCCCAGCTCGGCGAGATCGGCCATCGCGCGCCGGGTGCCGCCGGCGAGCCACCCTGGGCGGTCTCGGCC
CTCGTGGAGGGCGCTCTGGGCGAGGTCCGCCTTGGCACGGGCTACGATCCGGTGCAGGAAGGCGGGGTGCGGCGCTGCCG
CATCGACGGCGAGCCTGCGCCTTCCGCCAACGCCTTCCTCGACCATCTGAAAGTGCTCTGGCTGACGCCGGAGATGGATG
GCCTGTTCCTGGGGCCGCCCGGCGACCGGCGGCGCTATCTCGACCGCCTCGTGTTGGCGGTGGACGGCGCCCACGGCACA
AGGGTCAACGGCCTGGAACGGGCGCTGCGCTCGCGCAACCGGCTTCTGGAGGAGAACGGCTCGGCGCGCTTCCTCGATGC
GGTGGAGCACGAGGTGGCGGAACTCGCCGTGGCGGTGGCGGCGGCGCGGCTGGAGACGGTGGCCCGGCTCGGCGCCGAGA
TCGCCGCCCACCGCGACGATGCTTCCCTCTTTCCCTTCGCGGAGCTGGCCCTGGATGGGGCGGTGGAGCGGCTCATCGCC
GTCCATCCCGCGCTGGAGGTGGAGGACCGTTACCGTGCCCTCCTGCGCGACAACCGCCCCCGCGACCGGGCGGCGGGCCG
CACGCTGGAGGGGCCGCACCTCACCGATCTGTCGGTGAGCCATGGCGAGAAGCAACTGCCCGCCGCGCGCTGCTCCACGG
GGGAGCAGAAATCCCTGCTCATCGGCCTCACCTTGTCCCATGCGCGGCTGGTGGCCTCCATGCAGGGCTTCTCGCCCATC
CTGCTTTTGGACGACGTGGTGGCCTATCTCGACGCCGCGCGCCGCACCGGCCTGTTCGAGGCCCTGGCCCGCCTCGGCGC
GCAGGCCTGGATGACCGGCGCCGACCCCACCGCCTTCTCCGCGCTGGACGGCGCCGAGCGCTTCGAGGTGGCGCCGGGGA
CGATCGGGCGGGCGTAA

Upstream 100 bases:

>100_bases
GAAGACCGGGGCCGTTGGGGTGAGGCGGAAGCATATGTCGATCGCCGTCGCGTATCCTGCGGGAACGGCCGCATCTGATT
GAAGACCGGCCCGCAGGTCC

Downstream 100 bases:

>100_bases
GGCGCGGGCTCCTCTTCTGAAAATGCACTGCGCTGTCGCTGCAGTGGGTCGGGGCACGATGTGAGGCCCCCGAACAACCT
CGCTTGACTCCCTCCTCACT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MPHAAIRKLTLTAFRSYPSAQVSVEDGPVVLTGPNGAGKTNILEALSFLSPGRGLRRAQLGEIGHRAPGAAGEPPWAVSA
LVEGALGEVRLGTGYDPVQEGGVRRCRIDGEPAPSANAFLDHLKVLWLTPEMDGLFLGPPGDRRRYLDRLVLAVDGAHGT
RVNGLERALRSRNRLLEENGSARFLDAVEHEVAELAVAVAAARLETVARLGAEIAAHRDDASLFPFAELALDGAVERLIA
VHPALEVEDRYRALLRDNRPRDRAAGRTLEGPHLTDLSVSHGEKQLPAARCSTGEQKSLLIGLTLSHARLVASMQGFSPI
LLLDDVVAYLDAARRTGLFEALARLGAQAWMTGADPTAFSALDGAERFEVAPGTIGRA

Sequences:

>Translated_378_residues
MPHAAIRKLTLTAFRSYPSAQVSVEDGPVVLTGPNGAGKTNILEALSFLSPGRGLRRAQLGEIGHRAPGAAGEPPWAVSA
LVEGALGEVRLGTGYDPVQEGGVRRCRIDGEPAPSANAFLDHLKVLWLTPEMDGLFLGPPGDRRRYLDRLVLAVDGAHGT
RVNGLERALRSRNRLLEENGSARFLDAVEHEVAELAVAVAAARLETVARLGAEIAAHRDDASLFPFAELALDGAVERLIA
VHPALEVEDRYRALLRDNRPRDRAAGRTLEGPHLTDLSVSHGEKQLPAARCSTGEQKSLLIGLTLSHARLVASMQGFSPI
LLLDDVVAYLDAARRTGLFEALARLGAQAWMTGADPTAFSALDGAERFEVAPGTIGRA
>Mature_377_residues
PHAAIRKLTLTAFRSYPSAQVSVEDGPVVLTGPNGAGKTNILEALSFLSPGRGLRRAQLGEIGHRAPGAAGEPPWAVSAL
VEGALGEVRLGTGYDPVQEGGVRRCRIDGEPAPSANAFLDHLKVLWLTPEMDGLFLGPPGDRRRYLDRLVLAVDGAHGTR
VNGLERALRSRNRLLEENGSARFLDAVEHEVAELAVAVAAARLETVARLGAEIAAHRDDASLFPFAELALDGAVERLIAV
HPALEVEDRYRALLRDNRPRDRAAGRTLEGPHLTDLSVSHGEKQLPAARCSTGEQKSLLIGLTLSHARLVASMQGFSPIL
LLDDVVAYLDAARRTGLFEALARLGAQAWMTGADPTAFSALDGAERFEVAPGTIGRA

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=373, Percent_Identity=26.2734584450402, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- InterPro:   IPR007406 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 40310; Mature: 40179

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00617 RECF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHAAIRKLTLTAFRSYPSAQVSVEDGPVVLTGPNGAGKTNILEALSFLSPGRGLRRAQL
CCCHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHH
GEIGHRAPGAAGEPPWAVSALVEGALGEVRLGTGYDPVQEGGVRRCRIDGEPAPSANAFL
HHHCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHCCCEEEEECCCCCCCHHHHH
DHLKVLWLTPEMDGLFLGPPGDRRRYLDRLVLAVDGAHGTRVNGLERALRSRNRLLEENG
HHHEEEEECCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC
SARFLDAVEHEVAELAVAVAAARLETVARLGAEIAAHRDDASLFPFAELALDGAVERLIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
VHPALEVEDRYRALLRDNRPRDRAAGRTLEGPHLTDLSVSHGEKQLPAARCSTGEQKSLL
HCCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCHHCCHHHCCCCCCCEEE
IGLTLSHARLVASMQGFSPILLLDDVVAYLDAARRTGLFEALARLGAQAWMTGADPTAFS
EEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHEECCCCCHHHH
ALDGAERFEVAPGTIGRA
HCCCCHHEEECCCCCCCC
>Mature Secondary Structure 
PHAAIRKLTLTAFRSYPSAQVSVEDGPVVLTGPNGAGKTNILEALSFLSPGRGLRRAQL
CCHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHH
GEIGHRAPGAAGEPPWAVSALVEGALGEVRLGTGYDPVQEGGVRRCRIDGEPAPSANAFL
HHHCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHCCCEEEEECCCCCCCHHHHH
DHLKVLWLTPEMDGLFLGPPGDRRRYLDRLVLAVDGAHGTRVNGLERALRSRNRLLEENG
HHHEEEEECCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC
SARFLDAVEHEVAELAVAVAAARLETVARLGAEIAAHRDDASLFPFAELALDGAVERLIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
VHPALEVEDRYRALLRDNRPRDRAAGRTLEGPHLTDLSVSHGEKQLPAARCSTGEQKSLL
HCCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCHHCCHHHCCCCCCCEEE
IGLTLSHARLVASMQGFSPILLLDDVVAYLDAARRTGLFEALARLGAQAWMTGADPTAFS
EEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHEECCCCCHHHH
ALDGAERFEVAPGTIGRA
HCCCCHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA